Peixin He

Zhengzhou University of Light Industry · School of Food and Biological Engineering

The prominent problem of continuous cropping obstacle has been frustrating the morel farming. To deepen the understanding on morel continuous cropping obstacle, the allelopathic effects of phenolic acid extracts from morel continuous cropping soils on growth and development of Morchella sextelata, M. eximia, M. importuna, pathogenic fungus Fusarium...

Verpa spp. are potentially important economic fungi within Morchellaceae. However, fundamental research on their mating systems, the key aspects of their life cycle, remains scarce. Fungal sexual reproduction is chiefly governed by mating-type genes, where the configuration of these genes plays a pivotal role in facilitating the reproductive proces...

Morels are one of the most highly prized edible and medicinal mushrooms worldwide. Therefore, historically, there has been a large international interest in their cultivation. Numerous ecological, physiological, genetic, taxonomic, and mycochemical studies have been previously developed. At the beginning of this century, China finally achieved arti...

Morels, which belong to the Ascomycete genus Morchella, are highly valued edible fungi treasured by gourmet chefs worldwide. Some species are saprotrophic and others are able to form facultative mycorrhizal-like associations with plant roots without establishing true ectomycorrhizal symbioses. In general, it is considered that the formation of asex...

羊肚菌早期的驯化栽培因受制于基础研究不足而稳定性差。近年来，得益于羊肚菌人工栽培的快速发展，我国在羊肚菌种质鉴定与选育、交配型基因与生活史、菌种老化与质控体系、营养代谢与组学等研究领域取得了系列进展，并在一定程度上支撑了羊肚菌栽培区域和面积的快速扩张。然而，目前羊肚菌栽培仍然存在着优良种质匮乏、整体产量不稳定、病害与连作障碍凸显等问题，相关基础研究亟待开展与加强。

Morel mushrooms are iconic ascomycetes that are economically valuable and have attracted the attention of a large number of scientific researchers around the world. However, the tendency of morel fungi to undergo heterokaryosis through plasmogamy is not well understood. In this study, three populations of stipe tissue isolates were isolated from as...

Strain aging has been mainly contributing to the "uncertainty" of Morchella farming. The situation calls for urgent quantitative assessment of strain aging in cultivated Morchella mushrooms. In this paper, systemic senescence of the productive strains of M. eximia, M. importuna, and M. sextelata was achieved through successive subculturing to provi...

Our previous study found that isolates from morel stipes showed a large proportion of mating type deletion, which implied the nuclear evolution and spatial heterogeneity. In this paper, the specific mating type genes of Mat1-1-1 and Mat1-2-1 in Morchella importuna were analyzed using the relative quantitative method of qRT-PCR to study the uneven n...

Fermented grain (FG), a complex and unique ecosystem, is the main microbial habitats, biochemical reaction system and direct source of flavor compounds for the Chinese strong-flavor Baijiu (CSFB) production. However, the dynamics of physicochemical properties, prokaryotic community and flavor compounds of FGs during the long-term fermentation proce...

Background Chinese strong-flavor baijiu (CSFB) accounts for more than 70% of all Chinese liquor markets. Diverse microbes in pit mud found in the fermentation pit play a key role in CSFB production. However, the effect of spatial location on the diversity and structure of the microbial community in pit mud is still poorly understood Results Prokary...

It is widely believed that the quality and characteristics of Chinese Strong-Flavour Liquor (CSFL) are closely related with the age of the pit mud, and CSFL produced from older pit mud tastes better. This study aims to investigate alteration and interaction of prokaryotic communities across an age gradient in pit mud. Prokaryotic microbes in differ...

The commercial production of Morchella mushrooms calls for urgent breeding of excellent varieties or strains with appropriate tools, such as protoplast fusion. However, the protoplast fusion in morels has not been studied. In this paper, interspecific hybridization between cultivated morels of M. importuna and M. sextelata by PEG-induced protoplast...

Production of a thermostable pullulanase by DO‐stat fed‐batch fermentation of recombinant Escherichia coli BL 21 was investigated in a 5 L of fermentor. The effect of three oxygen control strategies, glucose feedback, shifting fermentor pressure, and adding oxygen‐enriched air, on cell growth and pullulanase expression were examined. The oxygen‐tra...

Medium‐chain fatty acids (MCFAs) and their derivatives are important chemicals that can be used in lubricants, detergents, and cosmetics. MCFAs can be produced in several microbes, although production is not high. Dynamic regulation by synthetic biology is a good method of improving production of chemicals that avoids toxic intermediates, but chemi...

True morels (Morchella spp.) are edible, medicinal mushrooms which have recently been artificially cultivated in China but stable production remains a problem. Here, we describe complete and comprehensive transcriptome of Morchella importuna at the stages of vegetative mycelium (VM), initial sclerotium (IS) and mature sclerotium (MS) by deep transc...

The prominent problem of "uncertainty" has been frustrating morel farming since the commercial production of Morchella mushrooms was realized in 2012 in China. Spawn aging may be the main underlying reason. In this paper, aging in cultivated strains of M. importuna T4 and M. sextelata T6 was achieved by successive subculturing. Oxidative stress, my...

In March 2013, pileus rot disease was first observed on cultivated Morchella importuna. Infected ascomata were covered by white, velvety mycelia mainly on the pileus, and the infection resulted in malformed fruiting bodies. The causal pathogen was identified as Diploöspora longispora based on its morphology and the internal transcribed spacer of it...

The effect on different three carbon source (i.e. glucose, fructose and sucrose) on production, chemical characterization and antioxidant activity of exopolysaccharide (EPS) produced by Phellinus vaninii Ljup was investigated in this study. Amongst carbon sources examined, glucose and sucrose were favorable for the mycelia growth, while the maximum...

The commercial production of Morchella mushrooms, especially M. importuna, has been realized since 2012 in China, which facilitates the fundamental studies of Morchella spp. In this paper, the cytological characteristics at three stages of ascocarpic development and the nuclear behavior during meiosis and ascosporogenesis in cultivated strain M. im...

用4μg/mL 4′,6-二脒基-2-苯基吲哚（DAPI）对细胞核染色,采用激光共聚焦显微成像技术观察粗腿羊肚菌（Morchella crassipes）子囊孢子的萌发过程。新鲜的粗腿羊肚菌子囊孢子呈椭圆状,大小13.015.3μm×21.524.0μm,成熟子囊孢子的细胞核数量为1832个;CYM液体培养基,23℃培养6h后子囊孢子体积逐渐膨大至初始体积的1.72.9倍,泡囊化现象明显;612h开始形成芽管,泡囊逐渐消失,出芽点在椭圆形细胞的一端形成或两端先后形成,数量不等的细胞核由子囊孢子内迁至芽管中,形成多核丝状;芽管生长至2030μm后,在出芽点与孢子连接处形成隔膜,1224h后,随着芽管的持续生长,形成新的隔膜和菌丝细胞,菌丝细胞多核;芽管及初生菌丝直径68μm,约为子囊孢子直径的...

In this study, a high yield of crude polysaccharide (16.73 ± 0.756%) was extracted from the spent mushroom substrate of Lentinus edodes using a hot alkali extraction method. Two groups of polysaccharides (designated as LSMS-1 and LSMS-2) were obtained from the crude extract by size exclusion chromatography (SEC), and their molecular characteristics...

Pleurotus eryngii has been widely cultivated in China and massive spent mushroom substrate (SMS) has been discarded. To utilize spent substrate of P. eryngii effectively, SMS was extracted by hot alkali liquor and a high yield (12.18%) of crude polysaccharide (designated as CPS) was obtained, then a polysaccharide-protein complex (RPS) was purified...

In this study, the effects of supplementary of Tween 80 and acetone on secretion, structure and antioxidant activities of exopolysaccharide (EPS) of Lentinus tigrinus were investigated. It was found that Tween 80 and acetone displayed a stimulatory effect on EPS secretion. The EPSs obtained by the addition of Tween 80 (EPS-T), acetone (EPS-A) and c...

In recent years, the artificial cultivation of Morchella mushrooms that belong to Elata Clade, including Morchella elata, has been developed rapidly in China. However, the prominent problem of spawn aging has been frustrating the morel farming. In this paper, aging in M. elata was achieved from 12 to 17 subcultures and lifespan of 1536-2256h by suc...

The effects of carbon source on properties and antioxidant potential of exopolysaccharides (EPS) produced by Trametes robiniophila were investigated in this study. The results indicated that the EPS production varied with five different carbon sources. The predominant carbohydrate compositions in EPSs identified were glucose and mannose. Then, FT-I...

Ultrasound-assisted extraction (UAE) of polysaccharide from Maryland tobacco leaves was studied by response surface methodology. Furthermore, the crude polysaccharide was purified and two components (Fr-I and Fr-II) were obtained. FT-IR spectral analysis of the purified polysaccharide revealed prominent characteristic groups. The monosaccharide com...

The optimal culture conditions of laccase productions in submerged culture by Abortiporus biennis in a pilotscale bioreactor (300 L) were determined using the rotating simplex method. The optimal culture conditions were C/N ratio 25, agitation speed 200 rpm, aeration rate 1.5. In the optimal culture medium, the maximum laccase production was 1361 U...

A novel gene ST2026 encoding a putative carboxylesterase from the thermophilic crenarchaeota Sulfolobus tokodaii (named EstSt7) was cloned and functionally overexpressed in Escherichia coli. The recombinant enzyme was purified to homogeneity after heat treatment, Ni-NTA affinity and Superdex-200 gel filtration chromatography. EstSt7 showed maximum...

The optimal culture conditions of exopolysaccharides (EPS) production in submerged culture medium by Pleurotus geesteranus 5 (#) were determined using an orthogonal matrix method. The optimal defined medium (per liter) was 60.0 g maltose, 5.0 g tryptone, 1 mM NaCl, 5 mM KH(2)PO(4), and initial pH 6.0 at 28 °C. In the optimal culture medium, the max...

An extracellular polysaccharide (EPS) from Phellinus igniarius was purified and characterized and its production investigated. The preferred culture conditions for EPS production were pH 6, temperature 28°C, with sucrose (35.40 g/L) and soy extract (2.58 g/L) as best carbon and nitrogen sources, respectively. The maximum EPS concentration was 1.549...

Optimization of culture conditions for exopolysaccharide (EPS) by Funalia trogii in submerged culture was investigated using one-factor-at-a-time method and uniform design (UD). Under the optimized conditions, the maximum concentration of EPS was 8.68 g/l. After EPS was deproteinized by Sevag method, two groups of EPSs (designated as Fr-I and Fr-II...

The aim of this work was to investigate the fermentation optimization, molecular characterization, and antioxidant activity in vitro of exopolysaccharides (EPS) from Morchella crassipes in submerged culture. Firstly, an optimal medium for EPS production was obtained by single-factor experiment and central composite design as follows: maltose 44.79...

The optimal culture conditions of exopolysaccharides (EPS) production in submerged culture medium by Stropharia rugosoannulata 2# were determined using the orthogonal matrix method. The optimal defined medium (per liter) was 60.0 g sucrose, 6.0 g tryptone, 5 mM KH2PO4, and initial pH 7.0 at 28°C. In the optimal culture medium, the maximum EPS produ...

Phellinus mushrooms can be easily identified to genus level by morphological characters; however, the taxonomy of species is very confused. The sclerotium producing mushroom, Hypholoma tuberosum is not recorded occurring in China. In this paper, the internal transcribed sequence (ITS) of two wild occurring mushrooms, P. igniarius CBL and H. tuberos...

The Se-tolerance of different strains of Auricularia auricular-judaer (wooden ear) was assayed on agar plates, the effects of selenium and Se-enrichment were studied in submerged culture and cultivation by adding different amounts of sodium selenite into the media. The results suggested that although the mycelial growth of tested strains was restra...

Using stem and leaf of Flos Lonicerae (SLFL) as main substrates to cultivate Pleurotus ostreatus and Lentinula edodes were studied. The main components of SLFL were analysed, the suitable media and strains of both mushrooms were selceted. The results suggested that SLFL may be suitable for mushroom cultivation. Media with mixture of SLFL and cotton...

Interactions between Psilocybe fasciata and its companion fungus Acremonium strictum were analysed. The conidia of A. strictum were observed on stipe, flesh and gill of P. fasciata, which suggested that A. strictum is the fungicolous fungi or mycophilic fungi of P. fasciata. The microscopic observations of the interacting hyphae of P. fasciata and...