Olivier Haeberlé

Université de Haute-Alsace | UHA · Institut de Recherche en Informatique Mathématique Automatique et Signal (IRIMAS)

Fluorescence microscopy is a powerful technique in biology, because of the immense variety of markers now available. Compared to other methods, its resolution is however limited. In wide-field microscopy, the technique of structured illumination permits to improve the lateral resolution by a factor of two, even surpassing confocal microscopy, which...

We have developed a tomographic diffractive microscope, equipped with a fluorescence confocal scanner. We measure experimentally the lateral resolution using an edge method and by comparing tomographic images of the same samples with wide-field and laser scanning confocal microscopy images; a scanning electron microscope image serves as a reference...

Optical microscopy techniques are among the most used methods in biomedical sample characterization. In their more advanced realization, optical microscopes demonstrate resolution down to the nanometric scale. These methods rely on the use of fluorescent sample labeling in order to break the diffraction limit. However, fluorescent molecules’ photot...

In article number 2200029, Vasily Astratov and colleagues representing 27 research teams worldwide created a roadmap on label-free super-resolution imaging. Its scope spans from diffraction-limited interference detection techniques to methods allowing to overcome classical diffraction limit without using fluorescent markers, which are based on info...

Label‐free super‐resolution (LFSR) imaging relies on light‐scattering processes in nanoscale objects without a need for fluorescent (FL) staining required in super‐resolved FL microscopy. The objectives of this Roadmap are to present a comprehensive vision of the developments, the state‐of‐the‐art in this field, and to discuss the resolution bounda...

Tomographic Diffraction Microscopy (TDM) is an invaluable tool in mark-erless 3D imaging of biosamples. However, TDM lacks from information selectivity compared to other techniques. This presentation will discuss extensions of TDM to other imaging contrasts, which, combined with the refractive index information, allows bringing back selectivity.

Tomographic diffractive microscopy (TDM) based on scalar light-field approximation is widely implemented. Samples exhibiting anisotropic structures, however, necessitate accounting for the vectorial nature of light, leading to 3-D quantitative polarimetric imaging. In this work, we have developed a high-numerical aperture (at both illumination and...

In this work, we numerically study the behaviour of the single-mode homogeneously broadened laser versus a large perturbation of the steady state. Periodic behaviour develops for suitable values of the ratio of the population decay rates ℘ \wp , which has been analytically predicted by the analytical approach developed in the previous works, and fo...

Aims: Pre-exercise cold-water immersion affects physical performance under ambient environment, however the mechanisms leading to this decrease remains to be elucidated. The purpose was to determine whether short-term lower-body immersion in cold water could induce acute changes in the development of neuromuscular fatigue after high-intensity exer...

L’imagerie optique des systèmes biologiques a connu ces dernières années des développements spectaculaires, délivrant une quantité et une qualité d’informations qui, il y a une vingtaine d’années encore, relevaient du rêve pour les physiciens, les biologistes ou les médecins.Les systèmes d’imageries non conventionnelles permettent d’accéder à des g...

L’imagerie optique des systèmes biologiques a connu ces dernières années des développements spectaculaires, délivrant une quantité et une qualité d’informations qui, il y a une vingtaine d’années encore, relevaient du rêve pour les physiciens, les biologistes ou les médecins.Les systèmes d’imageries non conventionnelles permettent d’accéder à des g...

Tomographic Diffraction Microscopy (TDM) is a generalisation of Digital Holographic Microscopy (DHM), for which the illumination angle onto the sample is fully controlled, which has become a tool of choice for 3‐D, high‐resolution imaging of unlabelled samples. TDM makes it possible to obtain the optical field in both amplitude and phase for each i...

Freeform optical designs deal with off-axis field curvature, which can be corrected by using curved microdisplays, with a biconic shape. In this work, we first show using simulations how curved microdisplays permit increasing image quality, but may also improve the distortion, the system’s cumbersomeness and luminous flux in visual systems. Focusin...

Tomographic Diffraction Microscopy (TDM) is a tool of choice for high‐resolution, marker‐less 3D imaging of biological samples. Based on a generalization of Digital Holographic Microscopy (DHM) with full control of the sample's illumination, TDM measures, from many illumination directions, the diffracted fields in both phase and amplitude. Photon b...

Our team works on an original concept of Near to Eye Display for Augmented Reality applications. This device requires mathematical research to optimize the diffraction phenomenon that forms the images onto the retina. Diffraction is an optical effect strongly related to periodicity and very well formulated by mathematics in particular through Fouri...

We propose an unsupervised regularized inversion method for reconstruction of the 3D refractive index map of a sample in tomographic diffractive microscopy. It is based on the minimization of the generalized Stein’s unbiased risk estimator (GSURE) to automatically estimate optimal values for the hyperparameters of one or several regularization term...

Tomographic diffractive microscopy (TDM) is increasingly gaining attention, owing to its high-resolution, label-free imaging capability. Fast acquisitions necessitate limiting the number of holograms to be recorded. Reconstructions then rely on optimal Fourier space filling to retain image quality and resolution, that is, they rely on optimal scann...

Tomographic diffractive microscopy (TDM) is a holographic-based imaging technique, which provides the three dimensional images of unstained samples' complex refractive index distribution (RI), by numerically recombining sets of diffracted fields recorded at various illumination angles . Practically, tens to hundreds of interferograms are acquired,...

Our team works on a new concept of smart glasses for augmented reality applications. Our retinal projector consists of a dense waveguide network to transport light on the surface of a glass and an electrode network to extract light. The laser beams are extracted through holographic elements that direct light into the eye. The images are directly cr...

Due to the sequential nature of data acquisition, it is preferable to limit the number of illuminations to be used in tomographic diffractive microscopy experiments, especially if fast imaging is foreseen. On the other hand, for high-quality, high-resolution imaging, the Fourier space has to be optimally filled. Up to now, the problem of optimal Fo...

We present design considerations for the development of a retinal projection display based on pixelated holograms. Herein, we focused on the writing and the characterization of pixels, and a simulation of expected results is provided.

This special issue of the Journal of the Optical Society of America A (JOSA A) is devoted to the wide array of French researchers from universities and state research organisms, offering them the opportunity to share and showcase their current research in the fields of optics and imaging sciences to the global community.

Tomographic diffractive microscopy (TDM) has gained interest in recent years due to its ability to deliver high-resolution, three-dimensional images of unlabeled samples. It has been applied to transparent samples in transmission mode, as well as to surface studies in reflection mode. Mudry et al. [Opt. Lett. 35, 1857 (2010)OPLEDP0146-959210.1364/O...

Tomographic Diffractive Microscopy (TDM) is an advanced digital microscopic imaging technique, extending the capabilities of Digital Holographic Microscopy (DHM), which delivers 3D quantitative images of the index of refraction distribution within the observed sample. It is a two-step imaging approach based first on recording of multiple holograms...

The augmented reality (AR) industry requires both aesthetic designs and high performances of AR devices. This complex dilemma challenges R&D groups from all over the world to improve existing systems or propose new, breakthrough designs. The unconventional concept of direct retinal projection display may be one. It is based on see-through holograph...

Tomographic diffractive microscopy exhibits intrinsic features making it a method of choice for 3D high-resolution label-free imaging. However, these results are achieved at the cost of a heavy data acquisition/reconstruction process. This drawback can be circumvented for certain classes of samples. For example, axisymmetric samples, like optical o...

We propose a novel technique for spatial demodulation of mirror-assisted tomographic diffractive microscopy images. Demodulation takes into account symmetry introduced by this mirror-effect, to properly reassign information in Fourier space.

Tomographic diffractive microscopy allows for imaging unlabeled specimens, with a better resolution than conventional microscopes, giving access to the index of refraction distribution within the specimen, and possibly at high speed. Principles of image formation and reconstruction are presented, and progresses towards real-time, three-dimensional...

Microscopy techniques allowing observation of unlabeled samples have recently experienced a regain of interest. In particular, approaches based on recording of the optical field diffracted by the specimen, in amplitude and phase, have proven their capacities for biological investigations. When combined with variations of specimen illumination, tomo...

Fluorescence microscopy has become the reference technique in cellular imaging, thanks to the specificity of fluorescence labeling, its high resolution, and its ability to image living specimens. However, the use of fluorophores can induce side effects, marking can be difficult, and speed of acquisition is often limited, especially for three-dimens...

We propose a solution to produce an intraocular image without any optical system between a near-eye display and the human eye. We simulate projected image and discuss influence of display characteristics on this image. The comparison between projected images of our concept and an ordinary microdisplay highlights the interest of the concept.

A nanoscale layer of amorphized silicon is obtained by implantations with silicon ions through a P-doped FZ-silicon wafer material few nanometers below the wafer surface. After a controlled annealing, the amorphized silicon material is sandwiched between two layers of recrystallized silicon. Defects remain at the interface c-Si/a-Si/c-Si. Photolumi...

Tomographic diffractive microscopy allows for imaging unlabeled specimens, with a better resolution than conventional microscopes, giving access to the index of refraction distribution within the specimen, and possibly at high speed. Principles of image formation and reconstruction are presented, and progresses towards realtime, three-dimensional a...

The development of new nanomaterials, devices and systems is very much dependent on the availability of new techniques for nanometrology. There now exists many advanced optical imaging techniques capable of subwavelength resolution and detection, recently brought to the forefront through the 2014 Nobel Prize for chemistry for fluorescent STED and s...

Abstract. Far field optical nanoscopy has been brought to the forefront with the 2014 Nobel Prize for chemistry in fluorescent nanoscopy for revealing intra-cellular details of tens of nm. In this review, we present an improved classification scheme that summarizes the many optical nanoscopy techniques that exist. We place particular emphasis on un...

In order to evaluate a concept of intraocular projection display, we present the first results of our experiments on a multiple beam diffractive setup. The optical setup is described and the simulations of the diffraction for various aperture distribution are discussed.

We present a concept of lens-free near-eye display based on the association of waveguides, grating couplers and pixelated holograms. The imaging behavior uses multiple beam interference to produce an image on the retina. A first validation of the diffractive phenomenon is proposed.

We propose a model of depth camera based on a four-lens device. This device is used for validating alternate approaches for calibrating multiview cameras and also for computing disparity or depth images. The calibration method arises from previous works, where principles of variable homography were extended for three-dimensional (3-D) measurement....

In computational neuroscience, receptors, channels and more generally signaling pathways are often modeled with Markov state models to represent biochemical reactions, which are then implemented with bilinear equations. One of the goals of these models, once calibrated with experimental results is to predict the dynamics of the biological system th...

Recently, long n-alkyltrimethoxysilanes (H3C(CH2)nSi(OCH3)3) have proven to self-assemble into mesoscopically ordered passive lamellar films through an efficient solvent-free photoacid-catalysed sol–gel process. By using an analogue precursor architecture presenting a terminal ester group (H3COC(O)(CH2)10Si(OCH3)3), both functional and tuneable nan...

In previous works, we have extended the principles of homography€, defined by Zhang and Greenspan, for measuring height of emergent fibers on glass and non-woven fabrics. This method has been defined for working with fabric samples progressing on a conveyor belt. Triggered acquisition of two successive images was needed to perform the 3D measuremen...

The authors have developed a tomographic diffractive microscope that combines microholography with illumination from an angular synthetic aperture. It images specimens relative to their complex index of refraction distribution (index and absorption) and permits imaging of unlabelled specimens, with high lateral resolution. The authors now study its...

Tomographic diffractive microscopy allows for 3-D imaging of unlabelled specimens, giving access to their index of refraction distribution. Principles of the technique, and recent progress towards real-time acquisition/reconstruction/display of the images will be presented.

We have applied harmonic expansion to derive an analytical solution for the Lorenz-Haken equations. This method is used to describe the regular and periodic self-pulsing regime of the single mode homogeneously broadened laser. These periodic solutions emerge when the ratio of the population decay rate ℘ is smaller than 0:11. We have also demonstrat...

We have developed a tomographic diffractive microscope in reflection, which permits observation of sample surfaces with an improved lateral resolution, compared to a conventional holographic microscope. From the same set of data, high-precision measurements can be performed on the shape of the reflective surface by reconstructing the phase of the d...

We have developed a tomographic diffractive microscope in reflection, which permits to observe sample surfaces with an improved lateral resolution, compared to a conventional holographic microscope. From the same set of data, high precision measurements can be performed on the shape of the reflective surface, by reconstructing the phase of the diff...

Résumé : Le microscope optique, en particulier de fluorescence, est devenu un instrument-clé pour la compréhension des mécanismes biologiques, grâce à ces possibilités uniques d'imagerie en 3D, sur des spécimens vivants. Sa résolution reste cependant limitée quand on la compare aux résultats obtenus en microscopie électronique, ou par les technique...

Résumé : Le microscope optique est devenu un instrument-clé pour la compréhension des mécanismes biologiques, grâce à ces possibilités uniques d'imagerie en 3D, sur des spécimens vivants. Son champ d'utilisation s'étend aussi à d'autres domaines comme la physique des surfaces et interfaces, la métallurgie, la cristallographie ou la pétrographie, le...

Résumé : Le microscope optique est devenu un instrument-clé pour la compréhension des mécanismes biologiques, grâce à ces possibilités uniques d'imagerie en 3D, sur des spécimens vivants. Son champ d'utilisation s'étend aussi à d'autres domaines comme la physique des surfaces et interfaces, la métallurgie, la cristallographie ou la pétrographie, le...

Industrial textile fabric with functionalization always has a high standard requirement. Take polytetrafluoroethylene (PTFE) conveyor belt as an example, the base fabrics must be of first-rate quality, with no weaving faults or broken fibers on coated surface [1]. In a previous work [2], we have proposed an original method to measure the height of...

In systems biology, systems of kinetic reactions are generally used to model and simulate various biochemical pathways. These reactions are translated into ordinary differential equations, which are computationally resolved by numerical algorithms. Computation performance, defined by how fast the algorithm converges to a numerical solution of the s...

Phase microscopy techniques regained interest in allowing for the observation of unprepared specimens with excellent temporal resolution. Tomographic diffractive microscopy is an extension of holographic microscopy which permits 3D observations with a finer resolution than incoherent light microscopes. Specimens are imaged by a series of 2D hologra...

Fabric’s smoothness is a key factor in determining the quality of finished textile products and has great influence on the func- tionality of industrial textiles and high-end textile products. With popu- larization of the zero defect industrial concept, identifying and measuring defective material in the early stage of production is of great intere...

Effects of stimulation frequency and localization on mGluRI-mediated calcium and IP3 dynamics. (A, B) Temporal evolution of cytosolic calcium concentration generated by mGluRI activation in response to increasing release frequency (color lines) with mGluRI at 100 nm (A) or 1000 nm (B) from the release site. Calcium responses were normalized to the...

Detailed IP3 receptor kinetic model and its validation. (A) Kinetic model of the IP3 receptor (IP3R) incorporating calcium and IP3 binding and the open state (adapted from [23], [24], [55]). The model is composed of four independent identical subunits with an inhibitory Ca2+ binding site. Both IP3 and Ca2+ are required for receptor/channel opening....

Interactions between ionotropic and metabotropic receptors in glutamate-mediated calcium and IP3 transients in dendritic spines. (A, B) Histograms represent the quantification of the AUC for calcium dynamics generated by activation of mGluRI, ionotropic glutamate receptors (AMPA/NMDA), and all receptors (total spine response) with mGluRI located at...

Influence of glutamate transporters on extrasynaptic glutamate concentration (1000 nm from the release site). Changes in extrasynaptic glutamate concentration in the presence (green line) or absence (blue line) of glutamate transporters with presynaptic stimulation frequency of 2 Hz (A), 10 Hz (B), 50 Hz (C) and 100 Hz (D). Note that the presence o...

Effects of stimulation frequency and mGluRI location on the respective contribution of ionotropic and metabotropic glutamate receptors on integrated calcium and IP3 dynamics. (A, B) Histograms represent the AUC for calcium (A) and IP3 (B) dynamics generated by activation of mGluRI and ionotropic glutamate receptors (AMPA/NMDA) with mGluRI located a...

Effects of stimulation frequency, glutamate transporters, and mGluRI location on glutamate-mediated calcium and IP3 dynamics. (A, B) Temporal evolution of cytosolic calcium (A) and IP3 (B) concentrations generated by glutamate receptor (AMPA, NMDA and mGluRI receptors) activation in response to various stimulation frequencies (color lines) with mGl...

Reaction equations, rate laws, and kinetic parameters of the mGluRI and calcium dynamics models. (DOC)

Activation of several subtypes of glutamate receptors contributes to changes in postsynaptic calcium concentration at hippocampal synapses, resulting in various types of changes in synaptic strength. Thus, while activation of NMDA receptors has been shown to be critical for long-term potentiation (LTP) and long term depression (LTD) of synaptic tra...

A fabric’s smoothness is a key factor to determine the quality of textile finished products and has great influence on the functionality of industrial textiles and high-end textile products. With popularization of the ‘zero defect’ industrial concept, identifying and measuring defective material in the early stage of production is of great interest...

This study presents a new method to measure the emergent fibers of fabric for textile online monitoring. In this concept, the textile fabric including the emergent fiber is considered as a two-layer structure. A variable homography method is proposed to estimate the height of the fiber. A vision system using an industrial camera has been built to o...

Communication présentée au Colloque Visualisation, Image, Modélisation, Nancy, France, 15-17 mai 2001

Tomographic Diffractive Microscopy is a technique, which permits to image transparent living specimens in three dimensions without staining. It is commonly implemented in two configurations, by either rotating the sample illumination keeping the specimen fixed, or by rotating the sample using a fixed illumination. Under the first-order Born approxi...

Tomographic Diffractive Microscopy (TDM) is a technique, which permits to image transparent living specimens without staining. For weakly diffractive samples, the three-dimensional distribution of the complex Refractive Index (RI) can be reconstructed from the knowledge of the measured scattered fields sampled under various viewing and illumination...

Using a 3D optical microscope, a profilometer, or a confocal scanning microscope, one can easily obtain a variety of surface data or 3D simulations1. However, in hairiness fiber testing, the scale of the fine fiber or broken filament is extremely small compared to the field of view. In this situation, conventional testing equipments are often usele...

We propose an image processing approach, based on variable homography, which can be used to measure in-plane fiber’s length on textile fabrics. We suppose that a fibrous structure can be considered as a two-layer structure and then show how the variable homography can estimate the length of the fiber defects. First, simulations are carried out to s...

N-Methyl-D-Aspartate receptors (NMDARs) play important physiological as well as pathological roles in the central nervous system (CNS). While NMDAR competitive antagonists, such as D-2-Amino-5-Phosphopentanoic acid (AP5) have been shown to impair learning and memory, the non-competitive antagonist, memantine, is paradoxically beneficial in mild to...

The authors have developed a tomographic diffractive microscope that combines microholography with illumination from an angular synthetic aperture. It images specimens relative to their complex index of refraction distribution (index and absorption) and permits imaging of unlabelled specimens, with high lateral resolution. The authors now study its...