Nitin Singhal

National Agri-Food Biotechnology Institute · Department of Food Science

Porous materials are highly explored platforms for fertilizer delivery. Among porous materials, metal–organic frameworks (MOFs) are an important class of coordination polymers in which metal ions and organic electron donors as linkers are assembled to form crystalline structures with stable nanoporosity. Selected amino acids were inherently found t...

Sepsis is a critical disease caused by the abrupt increase of bacteria in human blood, which subsequently causes a cytokine storm. Early identification of bacteria is critical to treating a patient with proper antibiotics to avoid sepsis. However, conventional culture-based identification takes a long time. Polymerase chain reaction (PCR) is not so...

Pseudomonas aeruginosa (P. aeruginosa) infection is becoming a severe health hazard and needs early diagnosis with high specificity. However, the non-specific binding of a biosensor is a challenge to the current bacterial detection system. For the first time, we chemically synthesized a galactose tripod (GT) as a P. aeruginosa-specific ligand. We c...

This book chapter gives an overview of the quartz crystal microbalance sensor (QCM) and how it can be used to detect pathogenic bacteria. The QCM sensor can be assumed as a mechanical transducer for a biosensor device that converts target analytes’ mass into an electrical signal. The detection of microorganisms with a QCM sensor has applications in...

The invention discloses a novel method for production of D-psicose (or D-allulose) by employing a nanobiocatalyst, developed by covalent attachment of Smt3-d-psicose 3-epimerase enzyme molecules onto the surface of magnetic iron oxide nanoparticles by covalent attachment, to treat D-fructose or D-fructose containing biomass feedstock. The nanobioca...

Amyloid-β (Aβ) aggregation plays a key role in the pathogenesis of Alzheimer's disease (AD). Along with this, the presence of redox-active metals like Cu2+ further enhances Aβ aggregation, oxidative stress, and cellular toxicity. In this study, we have rationally designed, synthesized, and evaluated a series of triazole-peptide conjugates as potent...

Foodborne diseases have increased in the last few years due to the increased consumption of packaged and contaminated food. Major foodborne bacteria cause diseases such as diarrhea, vomiting, and sometimes death. So, there is a need for early detection of foodborne bacteria as pre-existing detection techniques are time-taking and tedious. Aptamer h...

Astrocytes have emerged as active players in the innate immune response triggered by various types of insults. Recent literature suggests that mitochondria are key participants in innate immunity. The present study investigates the role of ischemia-induced innate immune response on p65/PGC-1α mediated mitochondrial dynamics in C6 astroglial cells....

The global increasing burden of non-communicable diseases such as cancer and diabetes have generated an immediate need for the development of sustainable drugs. Transition metal complexes with their versatility can offer excellent drug candidature potency. With very few reports on the non-toxic and safe drug candidatures of transition metal complex...

This work deals with the fabrication of metallosurfactants derived cobalt oxide and hydroxide nanosuspensions (Ns) by microemulsion method and their antimicrobial, cytotoxic, genotoxic, antioxidant and cytostatic activity have been investigated. The methodology used is environmentally compliant as no external reducing agent was used. Three metallos...

In our continued efforts to develop metal based therapeutic agents, we have synthesized a novel copper(II) complex, [{Cu(hpdbal-sbdt)}2] (2) tethered with a biocompatible ONS²⁻ donor backbone [H2hpdbal-sbdt] (1) [H2hpdbal-sbdt is a tridentate ligand derived from S-benzyldithiocarbazate (Hsbdt) and 2-hydroxy-5-(phenyldiazenyl)benzaldehyde (Hhpdbal)]...

Nowadays, the pollution in water resources has become a major concern, both environmentally and in perspective of human health. The bioaccumulation of pollutants, especially heavy metal ions through the food chain, poses a hazardous risk to humans and other living organisms. Nanomaterials and their composites have been recognized for their potentia...

Study indicated that along with alterations in insulin signaling pathway, expression of hallmark genes of Alzheimer's i.e. APP and BACE1 was more in co-cultured neurons as compared to monoculture. Results from animal study suggested a remarkable decrease in insulin receptor and p-GSK3bser9/t-GSK3b expression. Behavioral data indicated significant l...

Abstract Hepcidin, master regulator of iron homeostasis, causes anemia under infectious and inflammatory conditions by reducing intestinal absorption of iron with decreased release of iron from macrophages and liver despite adequate iron stores leading to Anemia of Inflammation (AI). Many therapeutic trials have been carried out but none have been...

Evidence from animal studies categorizes sporadic Alzheimer’s disease (sAD) as a metabolic syndrome with accompanying cognitive deficits. Given that glial cells act as “silent partners” to neurons by providing trophic support and defense, the present study investigated the role of glia in sAD pathology. A streptozotocin (STZ)-induced glial-neuronal...

Background & Objective Type 3 diabetes (T3D) is chronic insulin resistant state of brain which shares pathology with sporadic Alzheimer’s disease (sAD). Insulin signaling is a highly conserved pathway in the living systems that orchestrate cell growth, repair, maintenance, energy homeostasis and reproduction. Although insulin is primarily studied a...

The excessive discharge of phosphate in water bodies is one of the primary factors causing eutrophication. Therefore, its removal is of significant research interest. The present study deals with the development and performance of highly effective phosphate-adsorbent. Here, we have synthesized MIL-100(Fe) metal-organic frameworks as a facile strate...

Microbe-derived enzymes such as xylanases, cellulases and amylases, are efficient at hydrolyzing plant biomass. Efforts to harness the functionalities of these enzymes towards applications in energy and fuel biosciences, and food and nutrition, continue apace in many laboratories. Given that enzymes derived from mesophile proteomes undergo facile d...

In the efforts to develop a biocompatible transition metal complex as a drug alike for some of the prevailing non-communicable diseases (NCDs) and communicable diseases (CDs), a novel binuclear NiII compound [{NiII(hpdbal-sbdt)}2] (2) has been synthesized by the reaction of Ni(OAc)2.4H2O and H2hpdbal-sbdt (1) [1 is a dibasic tridentate ONS2- donor...

Magnetite and magnetite core/shell (Fe3O4/SiO2) nanoparticles were synthesized and functionalized with two different alkyl chain length linkers that were 3-Phosphonopropionic acid (3-PPA) and 16-Phosphonohexadecanoic acid (16-PHDA). Xylanase (EC 3.2.1.8, endo-1,4-xylanase, endo-1), was immobilized on as synthesized bare and silica coated magnetite...

Cereal grains undergo huge storage loss, which is significantly due to microbial contamination, on the other hand nutrient deficiency also coexists. Grain moisture is the key for microbial contamination to occur, hence we envisage to coat the grain with edible hydrophobic moisture barrier “zein”. But the challenge to coat zein is the thickness, unt...

The aim of the study was to covalently immobilize Smt3-D-psicose 3-epimerase onto functionalized iron oxide magnetic nanoparticles. After immobilization, Km of the immobilized enzyme increased, however, Vmax was nearly the same as that of its free form, indicating that immobilization has no detrimental effects on its catalytic output. The covalent...

Sporadic Alzheimer's disease (SAD) is a progressive neurodegenerative disorder with dysfunctional insulin signalling and energy metabolism. Growing evidence supports that impairment in brain insulin responsiveness, glucose utilization and energy metabolism may be a major cause of amyloid precursor protein mishandling. A support for this notion come...

In the present study, we have synthesised mixed cuprous/copper oxide nanosuspensions by metallosurfactant based microemulsion technique. Three metallosurfactants were synthesized which includes two non-ionic double chained metallosurfactants with C12, C16 chains with coordinated copper i.e. Cudda and Cuhexa, respectively. Another cationic double ch...

The aim of the study was to covalently immobilize Smt3-D-psicose 3-epimerase onto functionalized iron oxide magnetic nanoparticles. After immobilization, Km of the immobilized enzyme increased, however, Vmax was nearly the same as that of its free form, indicating that immobilization has no detrimental effects on its catalytic output. The covalent...

Vascular dementia (VaD) is the leading cause of cognitive decline resulting from vascular lesions. Recent studies have shown that mitochondrial dysfunctions and oxidative stress are involved in cognitive decline. The aim of the present study was to evaluate the beneficial effects of resveratrol-loaded solid lipid nanoparticles (R-SLNs) in permanent...

In this paper, we report an ultrasensitive and selective technique for direct electrochemical sensing of cadmium (Cd) by zinc oxide nanopillars (ZONPs) synthesized using a facile chemical method. The synthesized ZONPs have been characterized in terms of their topological, morphological, elemental, structural, and optical properties using various mi...

A wide variety of new materials and their hybrids have emerged as novel adsorbents to help resolve contamination of heavy metals in water resources. In this research, copper oxide (CuO) nanoblades were synthesized in high yield using a facile chemical method. These synthesized CuO nanoblades were characterized for their topological, morphological,...

Sporadic Alzheimer’s disease (SAD) is a progressive neurodegenerative disorder with dysfunctional insulin signalling and energy metabolism. Growing evidence supports that impairment in brain insulin responsiveness, glucose utilization and energy metabolism may be a major cause of amyloid precursor protein mishandling. A support for this notion come...

Hepcidin, a peptide hormone, is a key regulator in mammalian iron homeostasis. Increased level of hepcidin due to inflammatory conditions stimulates the ferroportin (FPN) transporter internalization, impairing the iron absorption; clinically manifested as anemia of inflammation (AI). Inhibiting hepcidin-mediated FPN degradation is proposed as an im...

Novel magnetic nanoparticles coated with silica and gold were synthesized for immobilization of α-amylase enzyme and characterized with Fourier transform infrared spectroscopy, transmission electron microscopy. Effect of various limiting factors such as substrate concentration, temperature, and pH on the catalytic activity of enzyme was investigate...

Iron deficiency is one of the most prominent mineral deficiencies around the world, which especially affects large population of women and children. Development of new technologies to combat iron deficiency is on high demand. Therefore, we developed alginate microcapsule with encapsulated iron that had better oral iron bioavailability. Microcapsule...

Oxidative stress has for long been linked to the neuronal cell death in many neurodegenerative conditions. Conventional antioxidant therapies have been less effective in preventing neuronal damage caused by oxidative stress due to their inability to cross the blood brain barrier. Nanoparticle antioxidants constitute a new wave of antioxidant therap...

Glyoxalase-I (GLO-I) is a component of the ubiquitous detoxification system involved in conversion of methylglyoxal (MG) to D-lactate in the glycolytic pathway. MG toxicity arises from its ability to form advanced glycation end products (AGEs). GLO-I has been reported to be frequently overexpressed in various types of cancer cells. In this study, w...

Glyoxalase-I (GLO-I) is a component of the ubiquitous detoxification system involved in conversion of methylglyoxal (MG) to D-lactate in the glycolytic pathway. MG toxicity arises from its ability to form advanced glycation end products (AGEs). GLO-I has been reported to be frequently overexpressed in various types of cancer cells. In this study, w...

It is critical to design and synthesize plasmonic nanostructures that can generate very strong and quantitative surface-enhanced Raman scattering (SERS) signals that could be amplified by multiple laser wavelengths to realize the full potential of SERS nanoprobes. Here, we report the synthesis of Au-nanobridged nanogap cucumbers (Au-NNCs) from DNA-...

It is critical to design and synthesize plasmonic nanostructures that can generate very strong and quantitative surface-enhanced Raman scattering (SERS) signals that could be amplified by multiple laser wavelengths to realize the full potential of SERS nanoprobes. Here, we report the synthesis of Au-nanobridged nanogap cucumbers (Au-NNCs) from DNA-...

Cells are structural and functional unit of our body that control and maintain the function of all unicellular and multicellular organisms. The process of cell proliferation and differentiation plays a vital role from the time of embryogenesis to development of whole organism from single‐ or double‐cell embryo and continues its crucial role in main...

Altering the lectin properties by chemically modified glycoconjugates can have profound effect on their biological applications. In the present case, jacalin has been chosen to study the binding aspects toward glycoconjugates modified by connecting aromatic moieties through imine conjugation at their C-1- or C-2-positions. Out of 10 glycoconjugates...

Three glyco-conjugates, viz., L(1), L(2) and L(3), which differ either in their carbohydrate moiety or in their aromatic moiety or both, were synthesized and characterized and were shown to have beta-anomeric form based on (1)H NMR and optical rotation studies. Metal ion interaction studies carried out in solution by emission and absorption spectra...

Several C 1-imino conjugates of D-galactose, D-lactose and D-ribose, where the nitrogen center was substituted by the salicylidene or naphthylidene, were synthesized and characterized. Similar C 2-imino conjugates of D-glucose have also been synthesized. All the glyco-imino-conjugates, which are transition state analogues, exhibited 100% inhibition...

Several C(1)-imino conjugates of D: -galactose, D: -lactose and D: -ribose, where the nitrogen center was substituted by the salicylidene or naphthylidene, were synthesized and characterized. Similar C(2)-imino conjugates of D: -glucose have also been synthesized. All the glyco-imino-conjugates, which are transition state analogues, exhibited 100%...

A galactosyl-naphthyl-imine-based derivative, 1-(beta-D-galactopyranosyl-1'-deoxy-1'-iminomethyl)-2-hydroxynaphthalene (GNI), and a galactosyl-naphthyl-amine-based derivative, 1-(galactopyranosyl-1'-deoxy-1'-aminomethyl)-2-hydroxynaphthalene (GNA), possessing an ONO binding core were studied for their recognition of naturally occurring amino acids...

[graph: see text] 1-(Beta-D-galactopyranosyl-1'-deoxy-1'-iminomethyl)-2-hydroxynaphthalene (L1), possessing an ONO binding core, was found to be selective for Cu2+ ions in N-[2-hydroxyethyl]piperazine-N'-[2-ethanesulfonic acid] buffer, at concentrations < or = 580 ppb, at physiological pH by eliciting switch-on behavior, whereas the other ions, viz...

12. Ahuja, Rohit; Singhal, Nitin K.; Rao, Chebrolu P. Lectins: chemical, structural and biological aspects including drug targeting. Khimiya (Sofiya, Bulgaria) (2006), 15(4), 275-304