Nadine WiesmannUniversity Medical Center Mainz
Nadine Wiesmann
Dr. rer. nat.
About
43
Publications
6,658
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Introduction
Nadine Wiesmann currently works at the Department of Otolaryngology, Head and Neck Surgery, University Medical Center of the Johannes Gutenberg-University Mainz. Nadine does research in Cancer Research, Cell Biology, Material Sciences, and Molecular Biology. She is working on the proteins 'hnRNPK' and 'NPM1', on 'ZnO nanoparticles', the tumor microenvironment, and senescence.
Additional affiliations
September 2015 - present
University Medical Center Mainz
Position
- PhD Student
October 2013 - July 2015
University Medical Center, Mainz
Position
- Master's Student
April 2011 - September 2011
University Medical Center, Mainz
Position
- Bachelor's Student
Publications
Publications (43)
Objectives
The primary objective of this in vitro experiment was an assessment of proliferative capacity, metabolic activity, and potential cellular detriment of human periodontal ligament cells (hPDL) exposed to cigarette smoke (CS), electronic cigarette vapor (eCV), and heated tobacco product aerosol (HTP), or air (control).
Materials and method...
Objectives
This systematic review and meta-analysis examined the effects of electronic cigarettes on periodontal health compared to conventional cigarette smoke and a non-smoking population.
Materials and methods
MEDLINE, Embase, Web of Science, CENTRAL, and ClinicalTrials.gov were screened for literature. Eligibility criteria included clinical st...
Large bone defects such as those that occur after trauma or resections due to cancer still are a challenge for surgeons. Main challenge in this area is to find a suitable alternative to the gold-standard therapy, which is highly risky, and a promising option is to use biomaterials manufactured by 3D printing. In former studies, we demonstrated that...
The chorioallantoic membrane (CAM) assay is an increasingly popular method using a hen's egg as an experimental organism. Animal models have been established in scientific research for centuries. Yet, awareness of animal welfare in society rises, and the transferability of findings obtained in rodent models to human physiology is challenged. Thus,...
Radiotherapy is still an important pillar for the therapy of head and neck squamous cell carcinoma (HNSCC); however, local recurrences arise in 15-50% of patients. During radiotherapy, there is a risk that individual tumor cells survive and proliferate again after a period of dormancy. Growing evidence suggests that senescence may play a key role i...
Periodontitis is a chronic biofilm-associated inflammatory disease of the tooth-supporting tissues that causes tooth loss. It is strongly associated with anaerobic bacterial colonization and represents a substantial global health burden. Due to a local hypoxic environment, tissue regeneration is impaired. Oxygen therapy has shown promising results...
Abstract: (1) Background: Electrical stimulation is a promising alternative to promote bone fracture healing but with the limitation of tracking the osteogenesis progress in vivo. To overcome this issue, we present an opportunity to combine the electrical stimulation of a commercial titanium implant, which promotes osteogenesis within the fracture,...
Bone fracture healing is a multistep process, including early immunological reactions, osteogenesis, and as a key factor, angiogenesis. Molecules inducing osteogenesis as well as angiogenesis are rare, but hold promise to be employed in bone tissue engineering. It has been demonstrated that the bone sialoprotein (BSP) can induce bone formation when...
(1) This study investigated the whitening effect, cytotoxicity and enamel surface alterations induced by different over-the-counter (OTC) bleaching agents in comparison to hydrogen peroxide. (2) Human teeth (n = 60) were randomly assigned into 6 groups (n = 10), stained with coffee solution for 7 d, followed by a whitening period of 7 d with either...
Various local and systemic factors compromise oral wound healing and may lead to wound dehiscence, inflammation, or ulcers. Currently, there is a lack of topical therapeutical options. Thus, this study aimed to investigate the effect of Aloe vera (AV) and Rheum palmatum root (RPR) on oral wound healing capacity in vitro. The effect of AV and RPR on...
Introduction:
The effective induction of angiogenesis is directly related to the success of bone-substitute materials (BSM) for maxillofacial osseous regeneration. Therefore, the addition of pro-angiogenic properties to a commercially available bovine bone-substitute material in combination with hyaluronic acid (BSM+) was compared to the same bone...
Simple Summary
Cold atmospheric plasma (CAP) resembles a physical state of matter, best described as ionized gas. CAP has demonstrated promising anti-cancer effects. Despite their relevance for the treatment of solid tumors, effects of CAP on tumor vessels and tumor-blood-circulation are still insufficiently investigated. CAP exposure reduced the v...
Oral mucosa is used in various surgical fields as a graft for the reconstruction of tissue
defects. Tissue engineering of oral mucosa equivalents using autologous cells represents a suitable less burdensome alternative. The survival of the multilayered epithelium is essential for the functionality of the tissues in vivo. To ensure its functionality...
Due to the demographic change, medicine faces a growing demand for tissue engineering solutions and implants. Often, satisfying tissue regeneration is difficult to achieve especially when co-morbidities hamper the healing process. As a novel strategy, we propose the incorporation of zinc oxide nanoparticles (ZnO NPs) into biomaterials to improve ti...
Objectives
For many years, tumor development has been viewed as a cell-autonomous process; however, today we know that the tumor microenvironment (TME) and especially cancer-associated fibroblasts (CAFs) significantly contribute to tumor progression. Caveolin-1 (Cav-1) is a scaffolding protein which is involved in several cancer-associated processe...
Core cross-linking of polymeric micelles has been demonstrated to contribute to enhanced stability that can improve the therapeutic efficacy. Photochemistry has the potential to provide spatial resolution and on-demand drug release. In this study, light-sensitive polypyridyl-ruthenium(ii) complexes were combined with polypept(o)ides for photocleava...
Simple Summary
Despite great advancements in modern cancer therapy, many patients suffer from local recurrence after initial treatment. The phenomenon of regrowth of tumor cells after several months or years is increasingly connected to therapy-induced cellular senescence. Our goal was to investigate the properties of tumor cells after survival of...
Toxicity tests in rodents are still considered a controversial topic concerning their ethical justifiability. The chick embryo chorioallantoic membrane (CAM) assay may offer a simple and inexpensive alternative. The CAM assay is easy to perform and has low bureaucratic hurdles. At the same time, the CAM assay allows the application of a broad varie...
Nanomaterials unveil many applicational possibilities for technical and medical purposes,
which range from imaging techniques to the use as drug carriers. Prior to any human application, analysis of undesired e�ects and characterization of their toxicological profile is mandatory. To address this topic, animal models, and rodent models in particula...
The chorioallantoic-membrane (CAM)-assay is an established model for in vivo tumor research. Contrary to rodent-xenograft-models, the CAM-assay does not require breeding of immunodeficient strains due to native immunodeficiency. This allows xenografts to grow on the non-innervated CAM without pain or impairment for the embryo. Considering multidire...
The effective management of tissue integration and immunological responses to transplants decisively co-determines the success of soft and hard tissue reconstruction. The aim of this in vivo study was to evaluate the eligibility of extracorporeal shock wave therapy (ESWT) with respect to its ability to modulate angiogenesis and immune response to a...
Human gingival fibroblasts (HGF) play a vital role in wound healing, oral cancer, and are among the first cells being exposed to e-cigarette vapor (eCV) or cigarette smoke (CS) during inhalation. Although the cell-damaging effect of CS has been well studied, the effects of eCV on gingival cells are still unclear. The aim of this in vitro study was...
Background: The chorioallantoic-membrane (CAM)-assay is used for versatile experimentation and eligible for the analysis of tumor angiogenesis, development and metastasis. In contrast to rodent xenograft models, the CAM-assay does not require breeding of immunodeficient strains for tumor experimentation due to native immunodeficiency. This allows x...
Despite improvements of radiotherapy and better outcomes of cancer patients resistances still limit the therapeutic success. The combined treatment of tumors by the use of irradiation as well as targeted therapies is a promising approach. By the use of a proteomic screening of lung and head and neck cancer cell lines we identified the heat shock pr...
The importance of zinc as a trace metal in the human body has long been overlooked. We now gradually discover that the impact of zinc on the health of our body might be as far-reaching as that of iron. Concurrently, nanomaterials containing zinc, in particular zinc oxide nanoparticles (ZnO NPs), are becoming increasingly attractive as innovative ag...
Nanoparticles are regarded as a promising therapeutic anti-cancer agent in treatment of head and neck tumours. On the way to clinical use the evaluation of toxicity is a central obstacle. However, animal experiments are associated with high costs and bureaucratic barriers. The Chorionallantoic Membrane-Assay (CAM-Assay) may offer an attractive alte...
Electrospun polymer mats are widely used in tissue engineering, wearable electronics, and water purification. However, in many environments the polymer nanofibers prepared by electrospinning suffer from biofouling during long-term usage, resulting in persistent infections and device damage. Herein, we describe the fabrication of polymer mats with C...
To fight resistances to radiotherapy, the understanding of escape mechanisms of tumor cells is crucial. The aim of this study was to identify phosphoproteins that are regulated upon irradiation. The comparative analysis of the phosphoproteome before and after irradiation brought nucleophosmin (NPM1) into focus as a versatile phosphoprotein that has...
Suppl. Fig. S1: Representative Western blots of the short-term measurements of the phosphorylation status of NPM1 in A549 cells after irradiation.
In Figure S1 are shown four representative Western blots that document the phosphorylation of NPM1 in A549 cells at threonine-199, threonine-234/237, serine-4, and serine-125 in nonirradiated control cel...
The predicted global cancer burden is expected to surpass 20 million new cancer cases by 2025. Despite recent advancement in tumor therapy, a successful cancer treatment remains challenging. The emerging field of nanotechnology offers great opportunities for diagnosis, imaging, as well as treatment of cancer. Zinc oxide nanoparticles (ZnO NP) were...
Background: Radiation therapy comprises a fundamental component of modern tumor treatment. Unfortunately, its success is limited by the development of radiation resistances. The emerging field of nanotechnology offers great opportunities for diagnosing, imaging, as well as treating cancer. Metal oxide nanoparticles in particular zinc oxide nanopart...
Organized three-dimensional (3D) nanomaterial architectures are promising candidates for applications in optoelectronics, catalysis or theranostics owing to their anisotropy and advanced structural features that allow tailoring their physical and chemical properties. The synthesis of such complex, but well-organized nanomaterials is difficult becau...
Radiotherapy is an important treatment option in the therapy of multiple tumor entities among them head and neck squamous cell carcinoma (HNSCC). However, the success of radiotherapy is limited by the development of radiation resistances. Heterogeneous nuclear ribonucleoprotein K (hnRNPK) is a cofactor of p53 and represents a potential target for r...
Unlabelled:
Irradiation resistance is a major obstacle of head and neck squamous cell carcinoma (HNSCC) therapy, limiting treatment success and patient survival. The aim of our experiments was to identify irradiation-regulated proteins as potential drug targets. Two established HNSCC cell lines (HNSCCUM-01T and HNSCCUM-02T) were treated with a sin...
Questions
Questions (2)
I digest human tumors that are growing in avian tissue and try to analyze them via flow cytometry. Therefore I am searching for an antibody to distinguish between the human tumor cells and all cell types stemming from the chicken. Could anybody recommend an antibody that is able to specifically label all chicken cells?
I incubate cells with nanoparticles and I know that ROS are generated. But I don't know where they exactly appear: in the cells or outside the cells. Therefore I am searching for a fluorescent dye that can measure ROS production outside cells, ergo does not enter the cells. Does anyone know a possibility to measure the ROS production outside cells or do I have to first incubate the cells with the media and then remove it and measure without the cells? Which dye would you recommend in a cell-free system?
