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Occurrence of Endocrine Disruption Chemicals (Bisphenol A, 4-Nonylphenol, and Octylphenol) in Muscle and Liver of, Cyprinus Carpino Common, from Anzali Wetland, Iran
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Phenolic endocrine disrupting chemicals are environmental pollutants with xenostrogen effects in wildlife and humans. The aim of this study was to determine 4-nonylphenol, Octylphenol, and Bisphenol A residues in various tissues of carp fish samples from Anzali wetland, Iran. 4-NP, OP, and BPA were detected with GC-MS in the muscle of fish from sampling location with maximal concentrations of 8.17, 9.67 and 5.87 μg/gdw, respectively. The highest concentrations of these compounds were found in the liver by HPLC. Since many endocrine disrupting substances were significantly lipophilic, distributing of these compounds into fish tissue has been correlated with lipid content.
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... Archive of SID 66 Taheri Mirghaed et al./ Plasma 17beta-estradiol and alkali-labile phosphoprotein levels in Tench Nasab, 2015). In contrast, concentrations of EDCs are considerably high in the Anzali Wetland (Mortazavi et al., 2012(Mortazavi et al., , 2013 due to releasing large amounts of untreated municipal and industrial wastewater into the wetland; as a result, fish could be exposed to the chemical compounds directly and indirectly in this wetland. ...
... Other researchers also found similar results over different species and in different regions (Hashimto et al., 2000;Folmar et al., 2001;Dick Vethaak et al., 2002). Mortazavi et al. (2012Mortazavi et al. ( , 2013 stated that the levels of EDCs (such as nonylphenol, octylphenol and bisphenol-A) at the Anzali Wetland were higher Taheri Mirghaed et al./ Plasma 17beta-estradiol and alkali-labile phosphoprotein levels in Tench compared to some other parts of the world such as rivers in China (Jin et al., 2010) and Japan (Isobe et al., 2001). When xenoestrogens present in water, they may changes aromatase activity (the enzyme converts androgens to estrogens) in males by increasing plasma E2 concentrations (Solé et al., 2003) and induction of vitellogenin. ...
Environmental pollutants are potentiate to disturb biological processes such as metabolism, growth and reproduction of aquatic organisms. These compounds are able to cause gonadal abnormalities, biased sex ratios and alteration in reproductive physiology in fish. The aim of this study was to examine plasma 17β-estradiol (E2) and alkali-labile phosphoprotein (ALP) levels in male and female Tench (Tinca tinca) from a polluted (the Anzali Wetland) and a non-polluted environments (the Amirkolayeh Wetland). Samples were collected over the maturation season of Tench between May and June 2017. The results revealed significant difference in mean ALP and E2 between genders in the polluted environment. However, the mean plasma ALP concentrations in male Tench of the polluted environment (39.46±1.02 µg/ml) was 45% of the average recorded in female (86.18±2.25 µg/ml) and was two times higher than the amount measured in males in the non-polluted environment (18.68±0.35 µg/ml). High concentrations of E2, were detected in the male samples from the Anzali Wetland. Mean plasma E2 concentrations for male in the Anzali Wetland was almost two times higher than male in the Amirkolayeh Wetland. The results indicate that the reproductive physiology of Tench was affected by contaminants found in the Anzali Wetland, a highly polluted area.
... Traditional methods of extraction include Soxhlet extraction [12], pressurized liquid extraction [13], and a combination of microwave-assisted extraction [14]. However, these techniques are time-consuming, solvent wasting, and require costly instruments [15]. ...
Owing to the difficult degradation of phenolic compounds, the existence of phenolic compound residues accumulated through the food chain in crustaceans and their effects on human health have garnered considerable and more attention. However, the current detection methods for phenolic compounds are not only complex and time-consuming but can also detect fewer types of phenols simultaneously. Thus, a simple, high-throughput method without derivatization for the determination of 18 phenolic compounds in crustaceans was developed using a modified QuEChERS sample preparation method combined with gas chromatography-mass spectrometry. The results indicated that the average recovery of the 18 phenolic compounds was 80.2–106.5% at three spiked levels, and the relative standard deviation was 2.6–12.4%. The limits of detection and quantitation were 0.001–0.014 and 0.003–0.042 mg/kg, respectively. Good linear relationships were observed for the phenolic compounds in their corresponding concentration ranges (R² > 0.999). The applicability of the method was assessed by analyzing 16 actual shrimp samples, among which five samples contained residues ranging from 0.013 to 0.042 mg/kg. Moreover, the establishment of this method has important theoretical and practical significance for the determination and control of the residue levels of phenolic compounds in crustaceans. In addition, it can provide a reference for the development of similar detection standard methods in the future.
... Its exposure to Clarias gariepinus resulted in anemia, increase in bilirubin content, increase in serum cortisol, leukocyte count increased, reduction in plasma protein levels, and increase plasma glucose levels. Mortazavi et al. (2013) determined octylphenol in various tissues of Cyprinus carpio and showed that highest concentration of OP was found in the liver tissue of carp. Bonefeld-Jorgensen et al, (2007) in their in vitro study have shown that OP has the potential to affect central endocrine pathways by modulating the functions of the nuclear receptors ER, AR, AhR and aromatase activity. ...
There are innumerable reports revealing that endocrine disrupting compounds modulates hormonal pathways regulating reproduction in teleost and other vertebrates. Besides introducing major impact on endocrine system leading to reproductive impairment and decline in local population of various aquatic species including teleosts, the biological effects of exposure to various endocrine disruptors has been investigated. Among various model systems for the detailed study, teleosts are the best for the assessment of fish reproduction, which is an ecological indicator of endocrine disruption. In the present review, we intend to highlight various aspects of toxic impact of alkylphenols on the fish reproduction and endocrine disruption.
... Furthermore, baby food packages were no longer able to contain BPA-based epoxy resins since 2013 (US Food and Drug Administration 2013). APs and BPA have been detected in marine, fresh water, wastewater treatment plants (WWTPs), sediments, drinking water, and rivers in worldwide studies (Ying et al. 2002;Soares et al. 2008;Stasinakis et al. 2008;David et al. 2009;Gatidou et al. 2010;Hawker et al. 2011;Zhang et al. 2011;Niu et al. 2015), and bioaccumulation studies have shown that APs and BPA can accumulate in environmental matrixes (in various species of algae, plants, invertebrates, and freshwater fish) even at low doses (Ahel et al. 1993;Snyder et al. 2001;Ying et al. 2002;Soares et al. 2008;Mortazavi et al. 2013;Staniszewska et al. 2014). Studies conducted in many countries have shown that APs and BPA can also be found in human tissues such as human blood, urine, breast milk, amniotic fluid, follicular fluid, placental tissue, semen, umbilical cord blood, fetal serum, and adipose tissues (Vandenberg et al. 2010;Li et al. 2013). ...
Biomonitoring studies are important tools to understand the effects of endocrine-disrupting compounds on human health. Up to now, there have been no biomonitoring and risk assessment studies conducted in Turkish population in which urinary bisphenol A (BPA), 4-nonylphenol (4-NP), and 4-t-octylphenol (4-t-OP) levels were measured simultaneously. The aim of this study is to measure urinary BPA, 4-NP, and 4-t-OP on Turkish population and conduct a risk assessment using urinary levels of chemicals of interest. During the study, liquid chromatography with tandem mass spectrometry (LC-MS/MS) was used to measure urinary levels of above-mentioned chemicals, and human biomonitoring was used as a risk assessment tool in 103 volunteers, living in Mersin Region, Turkey. Urinary BPA, 4-NP, and 4-t-OP were founded as 0.0079 μg/g creatinine, 0.0177 μg/g creatinine, and 0.0114 μg/g creatinine, respectively. The obtained estimated daily intakes (EDIs) were calculated as 0.095 μg/kg bw/day, 0.041 μg/kg bw/day, and 0.091 μg/kg bw/day, for BPA, 4-NP, and 4-t-OP, respectively. In conclusion, although no potential health risk due to BPA and 4-NP exposure was observed, there might be health risks associated with 4-t-OP exposure in the Turkish population.
The anadromous Hilsa, often known as the "Queen of Fishes" (Tenualosa ilisha), is the most valuable fishery in the Ganga-Hooghly delta estuary. Although BPA exposure has been shown to be harmful to aquatic organisms, no research has looked at the effects of BPA on the commercially valuable Hilsa shad of river Ganga. To close this information vacuum, we examined BPA levels in Hilsa fish from the Ganga estuary. Liver, muscle, kidney, and
gonads were all positive for BPA among the Hilsa fish of all ages. Liver BPA levels were highest in adult males (272.16 ± 0.38 ng/g-dw), and lowest in juveniles (5.46 ± 0.06 ng/g-dw). BPA concentrations in the Hilsa shad muscle were highest in reproductively mature females (196.23 ± 0.41 ng/g-dw). The study also discovered a correlation between fish development and BPA exposure, with higher levels of BPA being identified in adult Hilsa species. This is the first study to look at the impact of BPA pollution on aquatic ecosystems and fisheries, and it showed that Hilsa shad is contaminated with BPA and poses health hazards to human beings. The results, which demonstrate BPA contamination, are useful for protecting Hilsa in the river Ganga.
This work was focused on evaluating the occurrence of endocrine-disrupting compounds (EDCs) in fish muscles, such as bisphenol analogues, personal care products, including some UV-filters and musk fragrances, and selected pesticides. A total of 238 muscle samples of three fish species (Platichthys flesus, Mugil cephalus, and Dicentrarchus labrax) of an important estuary ending in the North East Atlantic Ocean (Douro River estuary, Portugal), in different seasons of the year, was analysed. M. cephalus was the species with a greater occurrence of contaminants, mainly in fishes collected during the spring. Bisphenol A was the main bisphenol analogue detected with an average content of 23.1 μg/kg wet weight (w.w.). Among chemicals used in personal care products, galaxolide, tonalide, and 2-ethylhexyl salicylate, were the most often found, being present in 15.9% (2.2–538.4 μg/kg w. w.), 4.6% (1.1–57.8 μg/kg w. w.) and 3.4% (3.9–56.1 μg/kg w. w.) of the samples, respectively. About 14% of the samples contained residues of at least one pesticide, being alachlor, aldrin, p,p’-DDT, permethrin, and prochloraz the main chemicals observed, varying from 0.1 μg/kg w. w. (p,p’-DDT) to 37.8 μg/kg w. w. (prochloraz). The daily intake estimates of the most frequent EDCs found in the fish muscles suggested that there are no health concerns, based on the recommended weekly consumption of fish for adults.
There is concern over the presence of a broad range of environmental pollutants known as endocrine disrupting chemicals (EDCs) and their disrupting consequences on normal reproductive processes. In the present study, probable abnormality was examined in immature and mature male and female of pike captured from the Anzali wetland (as polluted area with EDCs). A total of 38 pike from the Anzali wetland and 29 pike from the Amirkolaye wetland (as non-polluted area) were taken between October and November 2012. After macroscopic assessment of gonads, histological examination of gonads was performed using light microscope. Results of this study revealed no evidence of abnormality or intersex (the presence of female sexual cells in male gonads and vise versa) suggesting that gonad structure of pike is not affected by contaminants in the Anzali wetland recognized as highly polluted area.
Phenolic compounds can cause health problems in humans through the food chain. Considering that fish play an important role in human diets, we established a rapid, simple and high-throughput method for the determination of 18 phenolic compounds in fish based on a modified QuEChERS sample preparation method combined with GC-MS. The average recovery of the 18 phenolic compounds was 81.3-116% at 3 spiked levels, and the relative standard deviations, RSDr and RSDwR, were in the range of 1.1-11.3% and 1.5-12.2%, respectively. The limit of detection was 2.0-10.1 μg/kg. Satisfactory linear relationships (R2 > 0.998) were observed for the phenolic compounds in their corresponding concentration ranges. Moreover, the established method exhibited a high sensitivity, good stability, and reliability. The development of this method has an important theoretical and practical significance for establishing standards and to control the residue levels of phenolic compounds in fish.
Industrial expansion and increased water consumption have created water scarcity concerns. Meanwhile, conventional wastewater purification methods have failed to degrade recalcitrant pollutants efficiently. The present review paper discusses the recent advances and challenges in photocatalytic processes applied for industrial effluents treatment, with respect to phenolic compounds degradation. Key operational parameters including the catalyst loading, light intensity, initial pollutants concentration, pH, and type and concentrations of oxidants are evaluated and discussed. Compared to the other examined controlling parameters, pH has the highest effect on the photo-oxidation of contaminants by means of the photocatalyst ionization degree and surface charge. Furthermore, major phenolic compounds derived from industrial sources are comprehensively presented and the applicability of photocatalytic processes and the barriers in practical applications, including high energy demand, technical challenges, photocatalyst stability, and recyclability have been explored. The importance of energy consumption and operational costs for realistic large-scale processes are also discussed. Finally, research gaps in this area and the suggested direction for improving degradation efficiencies in industrial applications are presented. In the light of these premises, selective degradation processes in real water matrices such as untreated sewage are proposed.
In this study the actual presence of the suspected endocrine disrupter Bisphenol A (BPA) in water systems was studied in the Netherlands. BPA was shown to be present in Dutch surface water at levels up to 330 ng/l, and one occasional observation of 21 microg/l. During the three sampling periods, 60-80% of the samples, most from marine and estuarine locations, contained BPA levels below the limit of quantification (14-40 ng/l). At a selected number of locations the presence of BPA in fish was studied, which showed that BPA varied from 2 to 75 ng/g in the liver and 1 to 11 ng/g in the muscle. Based on present measured concentrations in surface water and on literature derived toxicity data it was concluded that ecotoxicological effects nor estrogenic effects are likely to occur in the field situation.
Little attention has been paid to the estrogenic-like compounds, such as 4-nonylphenol (4-NP) and its potential precursor nonylphenol ethoxylates (NPEOs), in China although its usage is huge. Water samples and corresponding drinking water samples were seasonally collected at five sites of each of the two main rivers in Chongqing Area. Individual nonylphenol ethoxylates (NPEOs) and 4-NP in the Changjiang River and Jialingjiang River were detected by normal-phase liquid chromatography electrospray ionization mass spectrometry and gas chromatography-mass spectrometry. The results indicated that of the five sampling points in the two rivers, NPEOs were the dominant pollutant in April and December with the similar distribution profile, and total NPEOs with different ethylene oxide lengths were 6.9-97.6 microg/L in April and 2.5-52.7 microg/L in December. However, NP was the dominant pollutant in July with a concentration of 1.7-7.3 microg/L. Corresponding drinking water samples derived from river water as source suggested that the conventional water treatment process used in the five waterworks could remove NPEOs from the source water with high removal efficiency (>99%). The 4-NP removal efficiency, however, varied in a range of 62% to 95%, leaving a significantly high concentration of NP (0.1 to 2.7 microg/L) in drinking water in July. Fish samples taken in December 2000 contained 4-NP of approximately 1.9 microg/g and NPEOs of 0.4-48.3 microg/g, with the highest concentration level found in liver.
Nonylphenols and related compounds are common products of biodegradation of a large group of nonionic surfactants, the nonylphenol polyethoxylates. Many of these compounds are known to be environmentally persistent and to elicit estrogenic response in both mammals and fish. In this study, nonylphenol (NP), nonylphenol monoethoxylate (NP1EO), and octylphenol (OP) were found in tissues of mature male flounder, Platichthys flesus (5−55 ng/g NP, 190−940 ng/g NP1EO, wet weight), and in tissues of juvenile flounder (30−180 ng/g NP, wet weight). These fish also showed detectable levels of the yolk protein vitellogenin in their plasma, indicative of estrogenic exposure. The compounds were also found in discharges from a major sewage treatment works (3000 ng/L NP, 45 000 ng/L NP1EO) and in sediments from two estuaries in north-east England; the highest levels from the highly industrialized Tees (1600−9050 ng/g NP, 125−3970 ng/g NP1EO, 30−340 ng/g OP, dry weight) and lower levels from the industrialized/urbanised Tyne estuary (30−80 ng/g NP, 160−1400 ng/g NP1EO, 2−20 ng/g OP dry weight). The implications of these findings for fish populations are discussed.
This article considers methods for the detection of oestrogenic effects in marine fish. As a marker for oestrogenic effects, plasma vitellogenin was determined in Atlantic cod, Atlantic salmon and goldsinny wrasse using ELISA with homologous or heterologous antisera. The sensitivity of marine fish to oestrogen exposure is species-dependent and cod is a suitable species for monitoring the effects of environmental oestrogens.
Stream flow in urban aquatic ecosystems often is maintained by water-reclamation plant (WRP) effluents that contain mixtures of natural and anthropogenic chemicals that persist through the treatment processes. In effluent-impacted streams, aquatic organisms such as fish are continuously exposed to biologically-active chemicals throughout their life cycles. The North Shore Channel of the Chicago River (Chicago, Illinois) is part of an urban ecosystem in which >80% of the annual flow consists of effluent from the North Side WRP. In this study, multiple samplings of the effluent and stream water were conducted and fish (largemouth bass and carp) were collected on 2 occasions from the North Shore Channel. Fish also were collected once from the Outer Chicago Harbor in Lake Michigan, a reference site not impacted by WRP discharges. Over 100 organic chemicals with differing behaviors and biological effects were measured, and 23 compounds were detected in all of the water samples analyzed. The most frequently detected and highest concentration (>100μg/L) compounds were ethylenediaminetetraacetic acid and 4-nonylphenolmono-to-tetraethoxycarboxylic acids. Other biologically-active chemicals including bisphenol A, 4-nonylphenol, 4-nonylphenolmono-to-tetraethoxylates, 4-tert-octylphenol, and 4-tert-octylphenolmono-to-tetraethoxylates were detected at lower concentrations (<5μg/L). The biogenic steroidal hormones 17β-estradiol, estrone, testosterone, 4-androstene-3,17-dione, and cis-androsterone were detected at even lower concentrations (<0.005μg/L). There were slight differences in concentrations between the North Side WRP effluent and the North Shore Channel, indicating minimal in-stream attenuation. Fish populations are continuously exposed to mixtures of biologically-active chemicals because of the relative persistency of the chemicals with respect to stream hydraulic residence time, and the lack of a fresh water source for dilution. The majority of male fish exhibited vitellogenin induction, a physiological response consistent with exposure to estrogenic compounds. Tissue-level signs of reproductive disruption, such as ovatestis, were not observed.
The distribution and bioaccumulation of steroidal and phenolic endocrine disrupting chemicals (EDCs) were studied in various tissues of wild fish species from Dianchi Lake, China. In muscle tissue, 4-tert-octylphenol, 4-cumylphenol, 4-nonlyphenol and bisphenol A were detected in fish from each sampling site, with maximal concentrations of 4.6, 4.4, 18.9 and 83.5 ng/g dry weight (dw), respectively. Steroids (estrone, 17β-estradiol 17α-ethynylestradiol and estriol) were found at lower levels (<11.3 ng/g dw) and less frequently in muscle samples. The highest concentrations of steroids and phenols were found in liver, followed by those in gill and the lowest concentration was found in muscle. The field bioconcentration factors (BCFs) of phenols were calculated in fish species ranged from 18 to 97. Moreover, the measured tissue concentrations were utilized in order to estimate water concentration of steroids (4.4-18.0 ng/L). These results showed that steroidal and phenolic EDCs were likely ubiquitous contaminants in wild fish.
Recent worldwide surveys have not only established incomplete removal of pharmaceuticals and personal care products (PPCPs) by sewage treatment plants, but also their presence in surface waters receiving treated sewage effluent. Those aquatic systems where sewage effluent dominates flow are thought to be at the highest risk for ecosystem level changes. The city of Regina, Saskatchewan, Canada (population 190,400) treats its sewage at a modern tertiary sewage treatment facility located on Wascana Creek. The Wascana Creek hydrograph is dominated by one major event: spring snow melt. Thereafter, creek flow declines considerably and in winter treated sewage effluent makes up almost 100% of stream flow. Four water surveys conducted on the creek from winter 2005 to spring 2007 indicated that PPCPs were always present, in nanogram and sometimes microgram per liter concentrations downstream of the sewage treatment plant. This mixture included antibiotics, analgesics, antiinflammatories, a lipid regulator, metabolites of caffeine, cocaine and nicotine, and an insect repellent. Not surprisingly, concentrations of some PPCPs were highest in winter. According to hazard quotient calculations and homologue presence, ibuprofen, naproxen, gemfibrozil, triclosan, erythromycin, trimethoprim, and sulfamethoxazole were present in Wascana Creek at concentrations that may present a risk to aquatic organisms. The continual exposure to a mixture of pharmaceuticals as well as concentrations of un-ionized ammonia that far exceed Canadian and American water quality guidelines suggests that Wascana Creek should be considered an ecosystem at risk. Although the Wascana Creek study is regional in nature, the results highlight the considerable risks posed to aquatic organisms in such effluent-dominated ecosystems.
Pharmaceuticals and personal care products are being increasingly reported in a variety of biological matrices, including fish tissue; however, screening studies have presently not encompassed broad geographical areas. A national pilot study was initiated in the United States to assess the accumulation of pharmaceuticals and personal care products in fish sampled from five effluent-dominated rivers that receive direct discharge from wastewater treatment facilities in Chicago, Illinois; Dallas, Texas; Orlando, Florida; Phoenix, Arizona; and West Chester, Pennsylvania, USA. Fish were also collected from the Gila River, New Mexico, USA, as a reference condition expected to be minimally impacted by anthropogenic influence. High performance liquid chromatography-tandem mass spectrometry analysis of pharmaceuticals revealed the presence of norfluoxetine, sertraline, diphenhydramine, diltiazem, and carbamazepine at nanogram-per-gram concentrations in fillet composites from effluent-dominated sampling locations; the additional presence of fluoxetine and gemfibrozil was confirmed in liver tissue. Sertraline was detected at concentrations as high as 19 and 545 ng/g in fillet and liver tissue, respectively. Gas chromatography-tandem mass spectrometry analysis of personal care products in fillet composites revealed the presence of galaxolide and tonalide at maximum concentrations of 2,100 and 290 ng/g, respectively, and trace levels of triclosan. In general, more pharmaceuticals were detected at higher concentrations and with greater frequency in liver than in fillet tissues. Higher lipid content in liver tissue could not account for this discrepancy as no significant positive correlations were found between accumulated pharmaceutical concentrations and lipid content for either tissue type from any sampling site. In contrast, accumulation of the personal care products galaxolide and tonalide was significantly related to lipid content. Results suggest that the detection of pharmaceuticals and personal care products was dependent on the degree of wastewater treatment employed.
A survey measuring concentrations of nonylphenol (NP) and its ethoxylates (NPEs) in fish was performed in the Kalamazoo River, Michigan, USA, in 1999. Of 183 fish analyzed, 59% had no detectable NP or NPE. Detected concentrations were reported to range from 3.3 (limit of detection) to 29.1 ng NP/g wet weight. To further explore the means of exposure of NP and NPE in the fish, concentrations of NP and its mono-through tri-ethoxylates (NPE(1-3)) were measured in fish, sediment, and water collected near two wastewater treatment plants on the Kalamazoo River in 2000. Samples were analyzed using exhaustive steam distillation with concurrent liquid extraction. Nonylphenol ethoxycarboxylates (NPE(1-3)C) were also analyzed in water. Concentrations of NP and NPEs in fish were less than the method detection limits (MDLs) in all the samples except one fish, which contained 3.4 ng NP/g wet weight, just above the detection limit of 3.3 ng/g. Three of 36 sediments and 1 of 24 water samples contained detectable concentrations of NP or NPE(1). NPE(2), NPE(3), and NPEC were not detected in water samples.
