Mustafa Numan

Adipose tissue functions both as an energy store and as an active endocrine organ secreting a large number of biologically important molecules called adipokines. Adipokines have been shown to be involved in the regulation of reproductive functions and the first adipokine identified was leptin. Recent research has shown that leptin is not only a pre...

Adipoz doku hem enerji deposu hem de adipokinler olarak adlandırılan biyolojik olarak önemli çok sayıda molekülü salgılayan aktif bir endokrin organ olarak işlev görmektedir. Adipokinlerin üreme fonksiyonlarının düzenlenmesinde yer aldığı kanıtlanmıştır ve tanımlanan ilk adipokin leptindir. Son yıllarda yapılan araştırmalar, leptinin beyine giden e...

Semen freezing and storing has been widely used in reproductive biotechnology, being applied to certain males of livestock breeds or animal species with economic value such as the Angora goat. The development of a semen extender with the cryoprotective agents can prevent the deterioration of sperm parameters after thawing. This study aimed to inves...

Background Obtaining functional sperm cells is the first step to treat infertility. With the ever‐increasing trend in male infertility, clinicians require access to effective solutions that are able to single out the most viable spermatozoa, which would max out the chance for a successful pregnancy. The new generation techniques for sperm selection...

Cryopreservation is widely used to preserve spermatozoa from azoospermic patients who have undergone testicular sperm extraction/aspiration. Freezing storage of spermatozoa is an important method to preserve male fertility. It is known that after some of spermatozoa obtained by surgical methods are used for IVF/ICSI, the remaining part is not disca...

Evaluation ZPBP1 in unexplained and asthenozoospermic patients

Advancements in technology and science have facilitated the development of various materials for use in different industries. These materials can range from large sizes to even nanometer dimensions. Nanomaterials or nanoparticles, which fall within the size range of 1 to 100 nanometers, have emerged as a prominent aspect of nanotechnology. Nanotech...

Advancements in technology and science have facilitated the development of various materials for use in different industries. These materials can range from large sizes to even nanometer dimensions. Nanomaterials or nanoparticles, which fall within the size range of 1 to 100 nanometers, have emerged as a prominent aspect of nanotechnology. Nanotech...

Background The aim of this study was to identify items that healthcare providers and/or patients consider important to include in a questionnaire for clinical trials and cohort studies in shoulder instability research. This could serve as a basis to develop a core outcome set for shoulder instability research. Methods Healthcare providers and pati...

Assisted reproductive technology (ART) is widely used in infertility treatments to achieve pregnancy. This technology includes in vitro fertilization (IVF) and microinjection (Intracytoplasmic Sperm Injection-ICSI) procedures. Today, ICSI is the most commonly used fertilization method for both severe male factor infertility and non-male-induced inf...

Değerli okuyucularımız; Sağlık bilimleri alanında özveri ile çalışan akademisyenlerimizin büyük emekleri ile hazırladığımız kitabımız, geniş kapsamlı bir yaklaşım ile bizlere güncel bilgiler sunmaktadır. Tıpta temel bilimler ile klinik bilimler birlikte çalışarak sürekli önemli gelişmeler kaydetmektedir. Konular üzerinde araştırma yaparken ona birç...

This study was aimed at investigating the effects of vitamin A (VITA), vitamin E (VITE), and combined β-carotene plus vitamin E (βCAR+VITE) injections on some fertility parameters in ewes. Estrus synchronization was performed by treating the ewes with intravaginal FGA sponges impregnated with 30 mg of fluorogestone acetate. On the days of the inser...

Asthenozoospermia, characterized by low sperm motility, is one of the most common causes of male infertility. While many intrinsic and extrinsic factors are involved in the etiology of asthenozoospermia, the molecular basis of this condition remains unclear. Since sperm motility results from a complex flagellar structure, an in-depth proteomic anal...

The reduction of spermatozoa survival time is a major problem of canine chilled sperm for artificial insemination. The current study looks at the possible advantages of chilling canine sperm to 4.00 ˚C for three days using Tribulus terrestris aqueous extract (TTAE). Three mixed-breed dogs were utilized to extract 24 ejaculates, which were then dilu...

In the presented study, the effects of ROCK inhibitor Y-27632, antifreeze protein III, and boron at two different doses were investigated on the spermatological parameters of Ankara buck semen after freeze–thawing. Ejaculates were collected from bucks using an electroejaculator during the breeding season. The ejaculates that showed appropriate char...

Aim: The purpose was to investigate the effects of various dosages of thymoquinone and resveratrol (5 and 20 mg/kg) on doxorubicin-induced hepatotoxicity in rats from a pathological standpoint. Materials and Methods: Eighty male Wistar Albino rats were used in this study. Animals were divided in to 10 groups: Control (physiological saline, PO); Dox...

Bu çalışmada Ovum Pick-Up (OPU) tekniği ile oosit verimi ile donörlerin Anti-Müllerian Hormon (AMH) konsantrasyonları arasındaki ilişkinin belirlenmesi amaçlanmıştır. Çalışmada 12 ila 15 aylık 10 sağlıklı Holstein düve kullanıldı. AMH ölçümleri Bovine VIDAS® Anti-Mülleian Hormon kitleri (Biomeriux, Marcy l'Etoile, Fransa) ile Mini Vidas cihazı kull...

Background: This study aimed to determine the effects of environmental temperature on the number and quality of oocytes and embryo production rates obtained by performing ovum pick up (OPU). Heat stress leads to long-term, short-term, visible, and invisible effects in dairy cows. Its effects on reproduction are evident in all stages, from oocyte de...

z Amaç: Bu çalışmada kültür vasatına ilave edilen L-Ergotiyonin ve Fetuin'in blastosist gelişimine etkisinin belirlenmesi amaçlanmıştır. Gereç ve Yöntem: Çalışmanın materyalini mezbahadan alınan ovaryum-lardan toplanan oositler oluşturmuştur. Maturasyon (IVM) medyumunda 22 saat inkübe edilen oositler fertilizasyon (IVF) medyumunda sperma ile 20-22...

Background Sperm cryopreservation has been widely used in the field of reproductive biotechnology. It applies to certain males of economic and scientific values, including livestock breeds or endangered animal species. The development of a semen extender with a low cryoprotectant concentration and an appropriate amount of trehalose and boron can pr...

This study was conducted to investigate the effect of semen extenders enriched with glutathione (GSH) on in vitro quality parameters and fertility of post-thawed turkey. In experiment 1, pools of semen diluted in glucose-based extender containing 0.5, 1, and 2 mM of GSH were cryopreserved. During the next step, a different variable such as motility...

Running title: Sperm proteome and ram fertility ABSTRACT Precise estimation of ram fertility is important for sheep farming to sustain reproduction efficiency and profitability of production. There, however, is no conventional method to accurately predict ram fertility. The objective of this study, therefore, was to ascertain proteomic profiles of...

Background: The purpose of this research was to compare the functional parameters of frozen-thawed Iranian Azari buffalo spermatozoa with imported semen samples of Italian Mediterranean buffalo (IMB) after the thawing process and 4 hours of incubation. Materials and methods: In this experimental study, a total of twenty-four ejaculates from four...

Brick masonry is one of the widely used materials for the construction of walls and columns in buildings. In many cases, the built walls and columns fail due to excess lateral loads, environmental degradation, and increased loading requirments due to changes in occupancy. In this paper, an experimental was conducted to understand the performance of...

This study was aimed to produce in-vitro embryos massively and at a lower cost. For this purpose, it was aimed to determine and compare the blastocyst growth rates of oocytes collected from slaughterhouse and by Ovum-Pick up (OPU) method. The animal material to be used in the OPU application of the study consisted of 10 Holstein breed heifers 15-25...

Since turkey reproduction is mainly through artificial insemination, short-term preservation of turkey semen is one of the most important issues in turkey reproduction management. The present study investigates the effects of glutathione (GSH) and trehalose on lipid peroxidation degree and turkey semen quality while being stored at 5 °C for 72 h. T...

Vitamin E is one of the most powerful antioxidants for prevention of cell damage resulting from cryopreservation, but its efficacy for cryopreserving brown trout sperm is still unclear. In this work, the protective effect of vitamin E on quality, fertilizing capacity, and DNA damage of brown trout (Salmo trutta macrostigma) sperm after cryopreserva...

According to developmental origins of health and disease hypothesis, we focused on maternal nutrition effects on testis alongside with brain. The aim was to evaluate feeding mice mothers with I) n-6 group, in which 300 mg Sunflower Oil (SO) and 50 IU vitamin E (standard levels of vitamin E (VE), II) n-3 group, in which 300 mg Fish Oil (FO) and 50 I...

Supplementing the extender with antioxidants with low molecular weight can enhance the quality of the post-thaw sperm during the freezing process. This study was aimed at determining the impacts of 3,4-dihydroxyphenyl glycol (DHPG) on the spermatozoa of the canine undergoing freeze-thawing process. In this study, 24 ejaculates were obtained from th...

Objective: This study was designed to assess the effects of using tris-soybean lecithin (TSL)-based extender supplemented with bovine serum albumin (BSA) on the quality of ram epididymal spermatozoa during refrigerated storage. Method: Epididymal sperm were collected from 22 Zandi rams, diluted in TSL-based extender at different concentrations (0%...

Background Freeze‐thawing process negatively affects ram sperm in terms of sperm quality, DNA integrity and antioxidant defence system. Thus, antioxidant supplementation of sperm during freeze‐thawing is suggested to improve sperm parameters. Objectives The aim of this study was to determine the effects of fetuin and trehalose added into ram seme...

This study aimed to evaluate the comparative effects of taxifolin hydrate and trehalose on the quality of frozen-thawed ram spermatozoa for the first time. Ejaculates collected from six mature rams were pooled, and divided to eight equal aliquots to extend them with different concentrations of glycerol (%5 and %3), taxifolin hydrate (10, 100, and 5...

Cryoprotectants are known to have protective effects against cryodamage to spermatozoa. In this study, the cryoprotective effects of two cryoprotectants (glycerol, ethylene glycol) and cryoprotectants/trehalose combinations on frozen-thawed ram spermatozoa were investigated at the ultrastructural level. For this purpose, ejaculates collected from K...

This study investigates the effects of cryopreservation and supplementation of buffalo's semen with Caffeic acid. It studies the effects of different Caffeic acid concentrations on cryopreservation capacity of the buffalo and evaluates their influence on various sperm parameters like motility, viability, progressive motility, sperm plasma membrane...

The objective of this study was to compare the effects of different concentrations of two different cryoprotectants (glycerol, G and ethylene glycol, EG) and trehalose (T), added to the semen extender, on post-thaw ram sperm parameters. Ejaculates, collected from 6 Merino rams, were pooled and evaluated at 37 °C. The pooled samples were divided int...

The freeze-thaw procedure causes irreversible structural and functional changes in human spermatozoa. In order to decrease the detrimental effects of cryopreservation and improve the quality of post-thawed spermatozoa, the constituents of the freezing solution attracted considerable attention. In this study, for the first time, we evaluated the eff...

Low temperature has been utilized to keep living cells and tissues dormant but potentially alive for cryopreservation and biobanking with great impacts on scientific and biomedical applications. However, there is a critical contradiction between the purpose of the cryopreservation and experimental findings: the cryopreserved cells and tissues can b...

Background: Boron has been considered as an essential nutrient for decreasing lipid peroxidation and improving antioxidant mechanism in different animal species. On the other hand, its effect on quality or DNA damage following cryopreservation process in fish sperm is still unclear. Objective: Experiments were designed to analyse the effect of a...

Cancer is a major public health problem, young cancer patients therefore undergo chemotherapy, and most of them may lose their fertility. DNA damage level provides important clues about the quality and reproductive potential of spermatozoa. In this study, we evaluated the levels of both DNA fragmentation and abnormal DNA integrity in the epididymal...

BACKGROUND: Cryopreservation of sperm from different fish species requires different protocols. Therefore, it is necessary to perform studies to establish reliable procedures for each species. OBJECTIVE: Experiments were designed to analyse the effect of different types of cryoprotectants on post-thaw motility, viability and fertility as well as cr...

Embryo transfer applications are widely used in our country and the world in order to train more efficient and stronger breeds in terms of genetic features. In order to perform a successful embryo transfer, it is necessary to work carefully in each step of the transfer process and to minimize oocyte and embryo loss during both manipulations and cul...

Our objectives were to assess sperm alteration and adipose tissue (AT) genes expression related to steroid metabolism subsequent to fatty acids consumption. Twenty‐nine mature male mice were divided into: fat diet (FD; n = 15) and the control group (n = 14). FD group was fed with low level of trans and saturated fatty acids source for 60 days. Sper...

The objective of this study was to examine the different concentrations of antipain and trehalose combination on post‐thawed quality of ram semen cryopreserved in tris extender. Ejaculates were collected from four rams using the artificial vagina, pooled at 37°C and diluted with (A0 Tre0: antipain 0 μM and trehalose 0 mM (Control); A10 Tre0; A50 Tr...

The present study was conducted to examine the protective role of arginine and trehalose on post-thaw bull sperm and oxidative stress parameters. Five ejaculates for each bull were used in the study. Each ejaculate, split into three equal aliquots and diluted at 37 °C with base extenders containing 2 mM arginine, 25 mM trehalose and no antioxidant...

Cryopreservation of the rabbit epididymal sperm and investigaiton of the sperm chromatin condensation using Toluidine Blue stain

It was determined that fetuin and hyaluronan supplementation did not provide any significant effect on the post-thaw subjective and CASA motility percentages and sperm motion characteristics, in comparison to the controls (P>0.05). Sperm acrosome and total abnormalities were similar in all groups (P>0.05). Groups M (hyaluronan+fetuin) and H (hyalur...

The aim of this study was to evaluate the effect of duration of CIDR device placement in vagina on estrus induction, corpus luteum status, plasma progesterone levels, follicle diameter, and pregnancy rate (PR) in Holstein dairy cows. Cows (n = 66) were randomly assigned by age, calving date and body condition score. In Group 1, CIDR was inserted on...

The objective of this study was to investigate the conception rates achieved with the transfer of in-vivo derived sexed and unsexed Holstein bovine embryos to appropriate recipients and to determine the accuracy of nonelectrophoretic PCR sexing. Seven-day-old embryos were derived in vivo by the nonsurgical flushing of the uterus. Before being vitri...

The aim of this study was to determine the effects of the antioxidants curcumin, ellagic acid and methionine on microscopic sperm parameters following the freeze-thawing of Merino ram semen. Ejaculates were collected via artificial vagina from four Merino rams and microscopically evaluated and pooled at 37 ◦C. The pooled semen samples were diluted...

The aim of this study was to determine the effects of the antioxidants curcumin, ellagic acid and methionine on microscopic sperm parameters following the freeze‐thawing of Merino ram semen. Ejaculates were collected via artificial vagina from four Merino rams and microscopically evaluated and pooled at 37 ◦C. The pooled semen samples were diluted...

The purpose of this study was to evaluate different treatment protocols to prevent embryonic death in ewes. A total of 180 Akkaraman crossbred ewes and 10 healthy rams were used as material. The ewes were divided into 3 equal groups, with each of the 3 groups then separated into 3 subgroups. Ewes in estrus, determined with teaser rams, were exposed...

In this study, it was aimed to investigate the effect of deslorelin, a GnRH analogue -which can postpone the sexual activity in some male animals-on the levels of plasma testosteron, testes size and side effects in Angora Bucks. As a animal material, 12 Angora male goat aged between 2-5 years and provided by Lalahan Animal Research Center. Animal w...

zet: Gamet hücrelerinin dondurulmasında kullanılan koruyucu maddeler (kryoprotektanlar) soğuk şoku ve donma esnasında gelişen diğer hasarlara karşı koruma sağlarlar. Kryoprotektanların hücrede toksik etkiye sahip olmaları, bu maddeler üzerindeki çalışmaları yoğunlaştırmış, ortama katılan eksternal kryoprotektanlarla, toksik etkimeler azaltılmaya ça...

A serological survey for detection of antibodies against avian influenza virus (AIV) subtype H9 in vaccinated layer flocks was carried out. Serum samples were divided into age groups A, B, C, D (commercial layers) and E, F, G, H (layer breeders). Haemagglutination inhibition (HI) test was performed to determine serum antibodies against AIV-H9 subty...

Summary : The aim of the study was to determine the effects of iron sulphate supplementation of diet containing 15 % cottonseed meal on the live weight gain, feed consumption, feed efficiency and some haematological parameters of broilers. Prior to experiment, a commercial broiler starter diet was fed to broiler chickens for two weeks. Than, 2-week...