Content uploaded by Munaff J. Abd Al-Abbas
Author content
All content in this area was uploaded by Munaff J. Abd Al-Abbas on Jun 17, 2016
Content may be subject to copyright.
Comparison study among isolates of Staphylococcus intermedius and S. aureus from various sources for their abilities to coagulate plasma of human, rabbit and dogs to hemolyse
... The coagulase test was performed with human plasma, because the human origin isolates have shared the property of coagulating human plasma with a higher percentage than other plasmas (Abd Al-Abbas, 2004;Bonar et al., 2018). ...
Abstract: Coagulase is considered as the main determinant to distinguish Staphylococcus aureus. The 3′ end coding region of the coagulase gene (coa) has a series of 81bp tandem repeats varying in number and sites of restriction enzyme among diverse isolates. The polymorphism of the coagulase gene among 45 MRSA isolated from burn wounds, otitis media and pneumonia in Basra city, Iraq, were investigated using PCR-RFLP analysis for 3′ end region with AluI enzyme revealing unique restriction patterns (18 patterns) not described in any previous studies. Particularly, pattern 1 was predominant. Different RFLP patterns of coa were shared among isolates from the different sampling sites.
The present study used the polymorphism of Coagulase gene to identify MRSA subtypes, estimate the efficiency of these methods in distinguishing the variable strains and compare these subtypes with the source of isolates.
... Although the source from which S.aureus isolated, is of importance in determining the type of plasma required to give the best clotting for coagulase test (Abd Al-Abbas, 2004), the clot formation by rabbit plasma using direct tube coagulase testing for the identification of S.aureus remains a good criterion, particularly, when the isolates are from different sources (Devriese et al., 2005; Qian et al., 2007). The frequency of coagulase negative staphylococci (CONS) in vagina and blood sources was higher than that of coagulase positive staphylococci (COPS). ...
ResearchGate has not been able to resolve any references for this publication.