Mohamed Hassan

Port Said University · Biology Dept.

The rapid expansion of miRNA research as cancer biomarkers has spurred the development of user-friendly miRNA-disease databases. However, integrating these bioinformatics tools into clinical practice requires innovative approaches. Digital innovation (DIN) represents the innovative link to integrate proposed ‘M3Cs standardized clinical validity fra...

Non-alcoholic steatohepatitis (NASH) is the clinically aggressive variant of non-alcoholic fatty liver disease. Hippo pathway dysregulation can contribute to NASH development and progression. The use of probiotics is effective in NASH management. Our aim is to investigate the efficacy of kefir Milk in NASH management via modulation of hepatic mRNA-...

Scorpions constitute a charismatic lineage of arthropods and comprise more than 2500 described species. Found throughout various tropical and temperate habitats, these predatory arachnids have a long evolutionary history, with a fossil record that began in the Silurian. While all scorpions are venomous, the asymmetrically diverse family Buthidae ha...

Objectives: This experimental study was conducted to look for a sensitive diagnostic panel for early detection of HCC. Methods: Combination of diethyl nitrosamine (DENA, 200 mg/kg, IP, once), two weeks later, CCl4(3ml/kg/week), subcutaneously, for 6 weeks) induced HCC in rats. Sixteen male Wistar rats were divided into 2 groups, control, and HCC. R...

Many studies have shifted the attention from the synthetic bioactive compounds to the naturally existing ones for the purpose of novel therapeutic agent(s). Such studies focused on the bioactive compounds from natural sources. The bioactive compounds from natural sources, which are developed in living systems, are supposed to be more biological fri...

Non-alcoholic steatohepatitis (NASH) is the clinically aggressive variant of non-alcoholic fatty liver disease. Hippo pathway dysregulation can contribute to NASH development and progression. The use of probiotics is effective in NASH management. Our aim is to investigate the efficacy of kefir Milk in NASH management via modulation of hepatic mRNA-...

A series of bis-thiazoles 5a–g were synthesized from bis-thiosemicarbazone 3 with hydrazonoyl chlorides 4a–g. Reaction of 3 with two equivalents of α-halocarbonyl compounds 6–8, 10, and 12a–d afforded the corresponding bis-thiazolidines 9, 11, and 13a–d, respectively. Condensation of bis-thiazolidin-4-one 9 with different aromatic aldehydes furnish...

Simple Summary Targeting topoisomerases has been widely used as anticancer therapeutics. Exposure to high temperature (hyperthermia) protects cells from the cytotoxic effect of topoisomerase-targeting therapeutics, yet the mechanism remains unknown. Here, we report that hyperthermia inhibits the nucleolytic processing of topoisomerase-induced DNA d...

Alzheimer's disease (AD) is characterized by alterations in monoamines, oxidative stress, and metabolic dysfunctions. We aim to assess the therapeutic impacts of roots or leaf extract from Urtica dioica (UD; stinging nettle) against scopolamine (SCOP)-induced memory dysfunction, amnesia, and oxidative stress in rats. Spatial memory was assessed by...

Diffuse large B-cell lymphoma (DLBCL) constitutes the largest percent of non-Hodgkin lymphoma (NHL). During B cell development, DNA double strand breaks are processed at immunoglobulin gene segments, which are recombined by mediating the excision, relocation, and re-ligation of these gene segments in a manner that utilizes several of the non-homolo...

Introduction: Hepatocellular carcinoma (HCC) is one of the most common cancers worldwide. Medemia argun (MA) fruits have been found to possess proanthocyanidins (PACs), having antioxidant activity. Methods: Intraperitoneal (IP) diethyl nitrosamine (DENA; 200 mg/kg, once) and carbon tetra chloride (CCl4, 3 ml/kg/week, subcutaneously, for 6 weeks) i...

Taxane is a family of front-line chemotherapeutic agents against ovarian cancer (OC). The therapeutic efficacy is frequently counteracted by the development of chemoresistance, leading to high rates of relapse in OC patients. The role(s) of microRNAs (miRNAs) in cancer chemoresistance had been supported by many evidences Epigenetic regulation by mi...

Aim: To assess isorhamnetin efficacy for diabetic kidney disease in a Type 2 diabetes mellitus rat model, through investigating its effect at the epigenetic, mRNA and protein levels. Materials & methods: Type 2 diabetes mellitus was induced in rats by streptozotocin and high-fat diet. Rats were treated with isorhamnetin (50 mg/kg/d) for 4 or 8 week...

Reliance on deep learning techniques has become an important trend in several science domains including biological science, due to its proven efficiency in manipulating big data that are often characterized by their non-linear processes and complicated relationships. In this study, Convolutional Neural Networks (CNN) has been recruited, as one of t...

The present study was designed to investigate the effect of Urtica dioica plant parts "leaves and roots" separately, known as stinging nettle, on propionic acid (PPA) induced autistic like rat model on the behavior, monoamines and bioenergetics changes in rats compared with the synthetic drug risperidone. Sixty male albino rats were divided into 5...

Scorpions constitute a charismatic lineage of arthropods and comprise more than 2,500 described species. Found throughout various tropical and temperate habitats, these predatory arachnids have a long evolutionary history, with a fossil record that began in the Silurian. While all scorpions are venomous, the asymmetrically diverse family Buthidae h...

ABSTRACT Colorectal cancer (CRC) is characterized by high heterogeneity and a complex microenvironment that leads to high inter-patient variability. Personalized management of CRC could address this. Accumulating data highlights the interaction between the CRC microenvironment and the immune system through different cells and receptors with a focus...

Birds are considered one of the least karyotypically examined animal groups due to their karyotype specificity, i.e. small chromosomes, a large diploid chromosome number and the separation of chromosomes into macro- and microchromosomes. The present work was aimed to investigate the number of chromosomes and their karyological and molecular genetic...

Ovarian cancer is the most aggressive female reproductive tract tumours. Taxane (paclitaxel; TX) is widely used for ovarian cancer treatment. However, ovarian cancers often acquire chemoresistance. MicroRNAs (miR) have been reported to mediate many tumours'chemoresistance. We investigated the role of miR-363 in the chemoresistance of the ovarian ca...

Background: Hepatocellular Carcinoma (HCC) is the leading cause of cancer deaths worldwide as well as in Egypt. We aimed to use Clustered Regulatory Interspaced Short Palindromic Repeats (CRISPR) gene editing technique to induce forced down-regulation of the circRNA which consequently modified miRNA expression in HepG2 cell line to prove the regula...

It is well known that many diseases throughout the world are well treated with medicinal plants which have great protective and therapeutic effects. The effect of crude ethanolic extract of Hyphaene thebaica (L) Mart (HT) on some hematologicalindices and some biochemical parameters in normal albino rats in addition to the histological studies on li...

Canagliflozin (CAN) is a sodium-glucose co-transporter 2 (SGLT2) inhibitor indicated to improve glycemic control in adults with type 2 diabetes mellitus. There is a little information about its effect on the cholinergic system that proposed mechanism for memory improvement occurring by SGLT2 drugs. This study aimed to estimate the effect of CAN as...

A hexanucleotide repeat expansion represents the most common genetic cause of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia, though the mechanisms by which the expansion cause neurodegeneration are poorly understood. We report elevated levels of DNA/RNA hybrids (R-loops) and double-strand breaks (DSBs) in rodent neurons, human cel...

Peripheral nerve injuries (PNI) are a frequent and disabling condition which affects 13 to 23 per 100,000 persons each; it remains a major cause of morbidity and social disruption. The aim of this study is to evaluate the possible therapeutic potency of melatonin (100 mg/kg) or resveratrol (100 mg/kg) in enhancing the recovery after Schwann cells t...

The ISSR-PCR and karyological analysis methods are successfully used to detect the genetic variations between the different species of fishes, especially these belonging to the same family. The aim of this study was to determine the karyotypes and molecular genetic variations of three species of family Osphronemidae, Trichogaster trichopterus, Tric...

The number of cytogenetic studies of fresh water fishes was increased in the recent years. Fish groups, such as Family: Characidae, comprises many of fishes which have economic importance. In the present study, the metaphase chromosomes and their karyotypes have been studied in three species of that family by using cytogenetic analysis. Fish specie...

Camptothecin (CPT), a topoisomerase I (TOP1) inhibitor, exhibits anti-tumor activity against a wide range of tumors. Redundancy of TOP1-mediated repair mechanisms is a major challenge facing the efficiency of TOP1-targetting therapies. This study aims to uncover new TOP1 targeting approaches utilising a selection of natural compounds in the presenc...

MicroRNAs (miRNAs) have been reported to regulate the development of chemoresistance in many tumors. Stathmin 1 (STMN1) is a microtubule-depolymerizing molecule, involved in chemo-response; however, the mechanism of its regulation is unknown. Herein, the immunohistochemical study indicated significant upregulation of the STMN1 in the ovarian cancer...

Apoptosis is the programmed cell death which maintains the healthy survival/death balance in metazoan cells. Defect in apoptosis can cause cancer or autoimmunity, while enhanced apoptosis may cause degenerative diseases. The apoptotic signals contribute into safeguarding the genomic integrity while defective apoptosis may promote carcinogenesis. Th...

In continuation of our interest in phytochemical screening of the Egyptian flora for potential drugs, the reinvestigation of the methanolic extract of the roots of Solanum diphyllum, which grows naturally in the south of Egypt and is recorded as new to the Egyptian flora, afforded an interesting, highly cytotoxic compound, named 3-O-(beta-D-glucopy...

We aimed to investigate whether MIR31 is an oncogene in human endometrial cancer and identify the target molecules associated with the malignant phenotype. We investigated the growth potentials of MIR31-overexpressing HEC-50B cells in vitro and in vivo. In order to identify the target molecule of MIR31, a luciferase reporter assay was performed, an...

Abstract: The Metaphase chromosomes of three marine fishes species of genus Epinephelus (Epinephelus aeneus, Epinephelus marginatus and Epinephelus costae (Family: Serranidae) and their karyological and molecular genetic relationships have been studied. All samples were collected from Egyptian Mediterranean Sea Coast. All three species have the sa...

Ovarian cancer is one of the most aggressive female reproductive tract tumors. Paclitaxel (PTX) is widely used for the treatment of ovarian cancer. However, ovarian cancers often acquire chemotherapeutic resistance to this agent. We investigated the mechanism of chemoresistance by analysis of microRNAs using the ovarian cancer cell line KFr13 and i...

The present investigation report the effect of a bradykinin-potentiating factor (BPF) on gentamicin-induced oxidative stress in rat liver and kidney. BPF is a peptide fraction isolated from the venom of the Egyptian scorpion (Buthus occitanus) has been demonstrated to have antioxidant, free radical scavenger and anti-inflammatory effects. Thirty ma...

Clusterin is a cytoprotective chaperone protein involved in numerous physiological processes, carcinogenesis, tumor growth and tissue remodelling. The purpose of this study was to investigate whether clusterin (CLU), an antiapoptotic molecule, could be a potential predictor molecule for ovarian cancer and whether or not targeting this molecule can...

Understanding the molecular events that lead to paclitaxel (TX) resistance is necessary to identify effective means to prevent chemoresistance. Previously, results from our lab revealed that secretory clusterin (CLU) form positively mediates TX response in ovarian cancer cells. Thus, we had interest to study the role of another non-secreted form (i...

Bradykinin and its related peptides are widely distributed in venomous animals, including scorpion. A peptide fraction isolated from the venom of the Egyptian scorpion Buthus occitanus was proved to have a bradykinin-potentiating activity. The aim of the present study was conducted to investigate whether the treatment with bradykinin potentiating f...

Multiple human papillomavirus (HPV) infection of the uterine cervix has been suggested as a risk factor for persistent HPV infection, resulting in the development of invasive cervical cancer. The aim of this study was to reveal the actual state of multiple HPV infection in Japanese patients with invasive cervical cancer. Sixty fresh-frozen invasive...

Overexpression of clusterin, an antiapoptotic molecule, has been reported to induce resistance to chemotherapy in a variety of cancer cell types. The aim of this study was to evaluate the significance of clusterin expression to predict response to platinum-based neoadjuvant chemotherapy and survival of patients with invasive cervical cancer who sub...

It has been reported that expression of Cyr61 decreased in endometrial cancers and cancer cell lines compared with normal endometrium, and forced expression of Cyr61 could suppress the growth of human endometrial cancer cells. However, in another report, Cyr61 was immunostained in most of endometrial cancer tissues analyzed. Thus, the aim of this s...

The aim of this study was to evaluate the prognostic significance of clusterin expression in invasive cervical cancer patients treated with radical hysterectomy and systematic lymphadenectomy. Invasive cervical cancer specimens were obtained from 52 patients who underwent radical hysterectomy and systematic lymphadenectomy at Hokkaido University Ho...

4371 Apoptotic pathways are consisted of many apoptosis­regulating proteins which are modified post­translationally and transcriptionally. Furthermore, we found that localization of apoptosis­regulating proteins was an important determinant of cancer cell fate. Lymphocytes expressed much Bcl­2 and a small amount of Bax. Surprisingly, all of so­ cal...