Manish Bhomia

Uniformed Services University of the Health Sciences | USUHS · Department of Pathology

Traumatic brain injury (TBI) is defined as an injury to the brain by external forces which can lead to cellular damage and the disruption of normal central nervous system functions. The recently approved blood-based biomarkers GFAP and UCH-L1 can only detect injuries which are detectable on CT, and are not sensitive enough to diagnose milder injuri...

Objective: Individuals with major limb amputation(s) frequently experience phantom limb sensations, which are described as vivid impressions of either parts or entire missing limb(s). Despite the high incidence and prevalence of phantom limb pain (PLP), the underlying pathophysiology of PLP remains poorly understood. The objective of this study wa...

This prospective, controlled, observational cohort study assessed the performance of a novel panel of serum microRNA (miRNA) biomarkers relative to findings on cervical spinal cord magnetic resonance imaging (MRI) in collegiate football players. There were 44 participants included in the study: 30 non-athlete control subjects and 14 male collegiate...

Stress-related sleep disturbances are distressing clinical symptoms in posttraumatic stress disorder patients. Intensely stressful events and their memories change rapid eye movement (REM) sleep in animal models. REM sleep varies with individual differences of stress resilience or vulnerability. The basolateral amygdala (BLA) is a primary mediator...

Transcriptomics, regional cerebral blood flow (rCBF), and a virtual reality (VR) based spatial motor task were integrated using mediation analysis in a novel demonstration of “imaging omics”. Data collected in NCAA Division I football athletes cleared for play before in-season training showed significant relationships in a) elevated levels of miR-3...

Neuroinflammation is a hallmark of several neurodegenerative diseases and disorders, including traumatic brain injury (TBI). Neuroinflammation results in the activation of glial cells which exacerbates the neuroinflammatory process by secretion of pro-inflammatory cytokines and results in disruption of glial transmission networks. The glial cells,...

Transcriptomics, regional cerebral blood flow (rCBF), and a spatial motor virtual reality task were integrated using mediation analysis in a novel demonstration of "imaging omics". Data collected in NCAA Division I football athletes cleared for play before in-season training showed significant relationships in a) elevated levels of miR-30d and miR-...

Traumatic brain injury (TBI) is currently considered one of the major causes of disability and death worldwide. The cellular and molecular changes of TBI pathology are dynamic and complex in nature. MicroRNAs (miRNA) are small endogenous RNA molecules that regulate gene expression at the posttranscriptional level. Several studies have shown a criti...

This prospective controlled observational cohort study assessed the performance of a novel panel of serum microRNA (miRNA) biomarkers on indicators of concussion, subconcussive impacts, and neurocognitive function in collegiate football players over the playing season. Male collegiate student football athletes participating in a Division I Football...

Background: Chikungunya virus (CHIKV) is a re-emerging pathogen that has caused widespread outbreaks affecting millions of people around the globe. Currently, there is no specific therapeutic drug against CHIKV, with symptomatic treatment only to manage the disease. Pi3-akt signaling has been implicated in infection of several viruses including tha...

Schizophrenia is a serious mental disorder with lifelong morbidity and increased mortality. Currently, the diagnosis of the disorder is based on patient history and clinical examination, but it has a low inter-rater reliability and validity. Various biological variables, such as event related potentials, hormonal levels, brain ventricular volume an...

Background Venezuelan equine encephalitis virus (VEEV) is an alphavirus in the family Togaviridae. VEEV causes a bi-phasic illness in mice where primary replication in lymphoid organs is followed by entry into the central nervous system (CNS). The CNS phase of infection is marked by encephalitis and large scale neuronal death ultimately resulting i...

Objective: To investigate the effect of heterogeneity in mTBI on miRNA expression in mouse brain and to identify molecular pathways targeted by the modulated miRNAs. Methods: A weight drop device was used to induce four increasing grades of mTBI. MiRNA expression was evaluated using TaqMan rodent miRNA arrays. Bioinformatics analysis was done using...

MicroRNAs (MiRNAs) are small endogenous RNA molecules and have emerged as novel serum diagnostic biomarkers for several diseases due to their stability and detection at minute quantities. In this study, we have identified a serum miRNA signature in human serum samples of mild to severe TBI, which can be used for diagnosis of mild and moderate TBI (...

MicroRNAs (miRNAs) are small, noncoding endogenous RNA regulatory molecules, which modulate gene expression either by mRNA degradation or translation suppression. MiRNAs in serum are resistant to repeated freeze thaw, enzymatic degradation and can survive extreme pH conditions. Due to these properties, miRNAs have the advantage over protein-based b...

MicroRNAs (miRNAs) are small, noncoding endogenous RNA regulatory molecules, which modulate gene expression either by mRNA degradation or translation suppression. MiRNAs in serum are resistant to repeated freeze thaw, enzymatic degradation and can survive extreme pH conditions. Due to these properties, miRNAs have the advantage over protein-based b...

Wars in Iraq and Afghanistan have highlighted the problems of diagnosis and treatment of mild traumatic brain injury (mTBI). MTBI is a heterogeneous injury that may lead to the development of neurological and behavioral disorders. In the absence of specific diagnostic markers, mTBI is often unnoticed or misdiagnosed. In this study, mice were induce...

Chapter 3. Molecular Mechanisms and Biomarker Perspective of MicroRNAs in Traumatic Brain Injury Nagaraja S . Balakathiresan , Anuj Sharma , Raghavendar Chandran , Manish Bhomia , Zhiqun Zhang , Kevin K . W . Wang , and Radha K . Maheshwari Citation Information Biomarkers of Brain Injury and Neurological Disorders Edited by Kevin K. W. Wang, Zhiqun...

Exposure to acute traumatic stress can cause permanent changes in neurological circuitry and may lead to the development of an anxiety disorder known as posttraumatic stress disorder (PTSD). Current diagnosis of PTSD is based on clinical or behavioral symptom assessment, however, these are not definitive due to overlapping symptoms with other psych...

Blast-induced traumatic brain injury (TBI) is of significant concern in soldiers returning from the current conflicts in Iraq and Afghanistan. Incidents of TBI have increased significantly in the current conflicts compared to previous wars, and a majority of these injuries are caused by improvised explosive devices. Currently, no specific technique...

Neuroinvasion of Venezuelan equine encephalitis virus (VEEV) and subsequent initiation of inflammation in the brain plays a crucial role in the outcome of VEEV infection in mice. Adhesion molecules expressed on microvascular endothelial cells in the brain have been implicated in the modulation of the blood brain barrier (BBB) and inflammation in br...

Table S1: Differential gene expression in the brain of uninfected, saline injected WT mice (n = 2) as compared to uninfected, saline injected IKO mice (n = 2). Average expression values of each gene are given. Where gene expression was detected in only one biological sample and not the replicates, the gene expression value is followed by POR (prese...

MicroRNAs (miRNA) are small RNA (approximately 22nts) molecules that are expressed endogenously in cells and play an important role in regulating gene expression. Recent studies have shown that cellular miRNA plays a very important role in the pathogenesis of viral infection. Venezuelan equine encephalitis virus (VEEV) is an RNA virus and is a memb...