Luiza Giacomolli Polesi

Federal University of Santa Catarina | UFSC · Departamento de Fitotecnia

Applying stress factors such as high temperatures during the different stages of somatic embryogenesis is important to uncover the molecular mechanisms of stress response and adaptation, and as a strategy to produce plants adapted to harsh environmental conditions derived from climate changes. In this sense, the present work aims to study the effec...

Applying stress factors such as high temperatures during the different stages of somatic embryogenesis is either important interesting to uncover the molecular mechanisms involved in stress response and adaptation, and as a strategy to produce plants adapted to harsh environmental conditions derived from climate changes. In this sense, the present...

This study aimed to establish a cryopreservation protocol for G. chacoensis embryogenic cultures (ECs) and to investigate the role of antioxidant enzymes activities during cryopreservation. The growth dynamics of cell suspensions were also investigated, followed by a phytotoxicity test to assess the ECs’ ability to tolerate the use of cryoprotectiv...

Somatic embryogenesis (SE) involves modifications of cellular, biochemical, genetic, and epigenetic patterns. Our work investigated proteins as markers of embryogenic response and characterized the redox state of embryogenic cultures (EC) of Guadua chacoensis. We identified a total of 855 proteins; 129 were up- and 136 down-accumulated in EC as com...

Rain forest in Brazil is home of around 50% of the world’s plant biodiversity and many species have a high economical potential. Butia eriospatha is an endemic species of the Brazilian Atlantic Forest and due to the anthropic intervention, it is in a vulnerable conservation status. In this species, plants are only propagated sexually by seeds which...

In this study, we focused on describing and characterizing the somatic embryogenesis (SE) process of Guadua chacoensis through morphohistological and biochemical analysis. For that, G. chacoensis in vitro shoots was used as explant in the induction experiments and we evaluated different Picloram concentrations (0–700 μM), explant pretreatments (con...

Changes in the chemical environment at the maturation stage in Pinus spp. somatic embryogenesis will be a determinant factor in the conversion of somatic embryos to plantlets. Furthermore, the study of biochemical and morphological aspects of the somatic embryos could enable the improvement of somatic embryogenesis in Pinus spp. In the present work...

Plant micropropagation comprises biotechnological tools used for the conservation and mass propagation of orchid species. However, there are few reports of its use for orchids of the subtribe Pleurothallidinae. The present study evaluated the effects of 6-benzylaminopurine (BAP) on shoots formation and development of Dryadella zebrina (Porsch) Luer...

Bamboos are one of the largest Poaceae groups and comprise about 1600 species distributed worldwide. Many of these species are sources of food, handicrafts, construction, charcoal, paper, and fiber. However, the obtainment of financially viable and high-quality seedlings of bamboo is not an easy task. Micropropagation has been successfully used to...

Formerly-embryogenic 8-year-old and 2-year-old embryogenic peach palm (Bactris gasipaes Kunth.) cultures were treated with 0, 4, 16, or 64 µM 5-azacytidine, an inhibitor of cytosine methylation, to evaluate its ability to restore embryogenic potential to old cultures and observe its effects on younger embryogenic tissue. Either 16 or 64 µM 5-azacyt...

Guadua chacoensis (Poaceae) is a woody bamboo native from the Atlantic forest biome. Morphogenetic and physiological studies are scarce in bamboos, and tissue culture-based biotechnologies tools can be used to investigate ultrastructure and physiological processes as well as to mass-propagate specific genotypes. This study evaluated the effect of l...