Lin Yao

Zhejiang University | ZJU · Institute of Environmental Science and Technology

Background Cholesteryl ester (CE) accumulation in intracellular lipid droplets (LDs) is an essential signature of clear cell renal cell carcinoma (ccRCC), but its molecular mechanism and pathological significance remain elusive. Methods Enabled by the label-free Raman spectromicroscopy, which integrated stimulated Raman scattering microscopy with...

Current methods for the early detection and minimal residual disease (MRD) monitoring of urothelial carcinoma (UC) are invasive and/or possess suboptimal sensitivity. We developed an efficient workflow named urine tumor DNA multidimensional bioinformatic predictor (utLIFE). Using UC-specific mutations and large copy number variations, the utLIFE-UC...

Cholesteryl ester (CE) accumulation is an essential signature of clear cell renal cell carcinoma (ccRCC), but its biological mechanism and pathological significance remain elusive. Here, enabled by label-free Raman spectromicroscopy, we confirmed aberrant CE accumulation in human ccRCC tissues but not in normal kidney tissues. To elucidate the unde...

Rapid identification and antimicrobial susceptibility testing (AST) of bacteria are key interventions to curb the spread and emergence of antimicrobial resistance. The current gold standard identification and AST methods provide comprehensive diagnostic information but often take 3 to 5 days. Here, a compound Raman microscopy (CRM), which integrate...

Background: Studies on biological functions of N6-methyladenosine (m6 A) modification in mRNA have sprung up in recent years. Previous studies have reported m6 A can determine mRNA fate and play a pivotal role in tumour development and progression. The zinc finger protein 677 (ZNF677) belongs to the zinc finger protein family and possesses transcr...

Background: N⁶-methyladenosine (m⁶A) is the most extensive messenger RNA modification. Despite recent advances in the biological roles of m⁶A, its role in the development and progression of renal cell carcinoma (RCC) remains unclear. Methods: In this study, we gained the transcriptome-wide m⁶A profile and gene expression pattern in RCC and paired a...

Upper tract urothelial carcinomas (UTUCs) are rare entities that are usually diagnosed at advanced stages. Research on UTUC pathobiology and clinical management has been hampered by the lack of models accurately reflecting disease nature and diversity. In this study, a modified organoid culture system is used to generate a library of 25 patient-der...

N⁶-Methyladenosine (m⁶A) is the most widespread internal RNA modification in several species. In spite of latest advances in researching the biological roles of m⁶A, its function in the development and progression of bladder cancer remains unclear. In this study, we used MeRIPty -55-seq and RNA-seq methods to obtain a comprehensive transcriptome-wi...

PurposeTo compare the tumor growth kinetics between sporadic clear cell renal cell carcinoma (ccRCC) and Von Hippel–Lindau disease-associated renal cell carcinoma (VHL-associated RCC). To analyze predictive markers for the growth rate of these two types of RCC.Methods The clinical data of patients with renal tumors who received active surveillance...

Purpose: To compare the tumor growth kinetics between sporadic clear cell renal cell carcinoma (ccRCC) and Von Hippel-Lindau disease-associated renal cell carcinoma (VHL-associated RCC). To analyze predictive markers for the growth rate of these two types of RCC. Methods: The clinical data of patients with renal tumors who received active surveilla...

Background: To evaluate and compare the natural history and growth kinetics of sporadic clear cell renal cell carcinoma (ccRCC) with those of ccRCC in von Hippel-Lindau disease (VHL). Methods: Sixty patients in the sporadic group with 61 tumors and 15 patients in the VHL group with 30 tumors whom all underwent delayed surgery after at least 12 m...

Purpose To explore the mechanism and efficacy of the modified bladder neck intussusception in laparoscopic radical prostatectomy (LSRP) on postoperative early continence. Methods We prospectively collected clinical information of prostate cancer patients who underwent LSRP with modified bladder neck intussusception (n = 10) and non-intussusception...

Purpose: Radical surgery is the preferred method for local high-risk and limited progressive prostate cancer in the routine clinical setting. However, current guidelines do not recommend neoadjuvant hormone therapy (NHT). Opinions regarding NHT vary among individual clinicians. According to the experience gained at our center, we explored the benef...

Background In China, upper tract urothelial carcinoma (UTUC) is less prevalent but more malignant in males. This study investigates the prognostic factors and causes of gender-based differences in Chinese populations. Methods Between 1999 and 2011, 687 UTUC patients who underwent surgery were utilized for this study. We evaluated the differences i...

Traditional laparoscopic radical prostatectomy is a treatment choice in many developing countries and regions for most patients with localized prostate cancer; however, no system for predicting surgical difficulty and risk has been established. This study aimed to propose a simple and standard preoperative classification system of prostate cancer u...

PurposeTo evaluate the long-term outcomes of laparoscopic ureteral reimplantation with extracorporeal tailoring and direct nipple ureteroneocystostomy for adult obstructive megaureter and compare the results with the open procedure. Methods From January 2007 to July 2013, 22 patients underwent laparoscopic and 28 patients underwent open ureteral re...

Here, we assessed the diagnostic accuracy of fluorescence in situ hybridization (FISH) for detecting aggressive upper tract urothelial carcinoma (UTUC). A total of 210 UTUC patients from a single hospital in China were enrolled in this retrospective study between 2012 and 2016. Patients were classified as FISH-positive or -negative based on FISH an...

Background To evaluate the safety and efficacy of the completely retroperitoneoscopic nephroureterectomy (CRNU), a retrospectively comparative study between completely and traditionally retroperitoneoscopic nephroureterectomy (TRNU) was done in a single center. Methods From January 2014 to December 2014, 107 patients with upper tract urothelial ca...

Purpose: To evaluate the association between tumor squamous and/or glandular differentiation and tumor biological characteristics and to validate the impact of these histologic variants on oncologic outcomes of UTUC patients. Methods: We retrospectively analyzed the data of 687 UTUC patients who underwent radical nephroureterectomy in our instit...

Objective. To present our experience of using transperitoneal subcostal access, Palmer's point (3 cm below the left costal margin in the midclavicular line), and its right corresponding site, in urologic laparoscopy. Methods. We used Palmer's point and the right corresponding site for initial access in 302 urologic surgeries (62 cases with prior su...

Previous reports about laparoscopic total pelvic exenteration (LTPE) are still limited. In the present study, we described our single-center experience of the initial 11 cases. Between April 2011 and September 2015, eight males and three females diagnosed as pelvic malignancies underwent LTPE by the same operation team. We retrospectively collected...

Background and purpose: To describe a novel and pure laparoscopic approach using a custom-made bulldog clamp with modified port placement for management of distal ureter during laparoscopic nephroureterectomy (LNU). Patients and methods: Between October 2013 and December 2014, 31 patients diagnosed with upper tract urothelial carcinoma (UTUC) we...

Objectives. Previous studies revealed an unclear correlation between the growth rate of renal cell carcinoma (RCC) and tumor grade and did not focus on certain histological subtype. This report investigated the correlation between the growth rate and tumor grade in clear cell RCC (ccRCC). Methods . We reviewed 60 patients with 61 ccRCC confirmed by...

Objective We introduce the innovative Institute of Urology, Peking University (IUPU) techniques for retroperitoneal laparoscopic surgery for the upper urinary tract, which have been published by Department of Urology, Peking University First Hospital and Institute of Urology, Peking University, and promote their widespread use in the field of urolo...

Objective To review retrospectively the clinical information of patients with renal cell carcinoma (RCC) and venous tumor thrombus, and to evaluate the changing from open to laparoscopic surgery and medium-term prognosis of these patients. Methods From Jan 2000 to Dec 2014, 276 patients were treated with renal cell carcinoma and venous tumor throm...

Objective To evaluate the association between tumor squamous and/or glandular differentiation and tumor biological characteristics, to validate the impact of these histologic variants on oncologic outcomes of UTUC patients. Methods We retrospectively analyzed the data of 687 UTUC patients who underwent radical nephroureterectomy in our institute,...

To investigate the natural history of renal cell carcinoma (RCC) with delayed treatment and to immunohistochemically analyze the correlation between some biomarkers and the growth rate of RCC. We reviewed our institutional databases to identify renal tumors which were confirmed to be RCC by delayed surgical treatment after at least 12 months of act...

We conducted this study to identify gene promoter methylation status and clinical predictors for upper tract urothelial carcinoma (UTUC) patients. Using methylation-sensitive polymerase chain reaction, we examined ten genes promoter methylation status in 687 UTUC patients. A methylated promoter of three genes to predict higher tumor stage (T3 and T...

To describe a modified suture technique for transperitoneal laparoscopic dismembered pyeloplasty (TPLDP) that can be consistently replicated. Between June 2010 and April 2014, 21 men and 7 women with primary pelviureteric junction obstruction underwent our modified TPLDP suture technique performed by the same surgeon. In our method, the dismemberin...

Objectives To investigate the incidence and treatment strategies for bilateral upper tract urothelial carcinoma (UTUC) and to compare the characteristic and oncologic outcomes of bilateral UTUC with those of unilateral tumors. Methods and materials The study included 892 consecutive patients with UTUC. Bilateral UTUC was defined as synchronous bil...

Introduction To present our experience of using transperitoneal subcostal access (Palmer’s point and the right corresponding site) in urologic laparoscopy. Material and methods We used Palmer’s point and the right corresponding site for initial access in 302 urologic surgeries (48 cases with prior surgeries). The record of these cases was reviewed...

Objectives To investigate the natural history of renal cell carcinoma (RCC) with delayed treatment, and immunohistochemically analyzed the correlation between some biomarkers and the growth rate of RCC. Patients and methods We reviewed our institutional databases to identify renal tumors which were confirmed RCC by delayed surgical treatment after...

Objective To accurately describe the growth of human renal clear cell carcinoma. In our earlier research, the growth model of renal tumors is linear and is not accurate. A better growth model is needed. Methods There patients under surveillance for more than ten years with renal tumors were analyzed. Tumor size and time were recorded in each obser...

There is currently no consensus about the pattern and risk factors of bladder recurrence after nephroureterectomy, especially in the Chinese population. We evaluated the pattern and risk factors based on data from a large Chinese center. The clinical and pathological data of 438 patients with upper tract urothelial carcinoma (UTUC), who underwent n...

To describe a novel technique using extracorporeal tailoring and an inverted ureteral nipple to perform laparoscopic transperitoneal ureteral reimplantation for adult obstructed megaureter and our initial experience with 10 patients. Ten patients (mean age 39.2 years [range, 21-62]) with adult obstructed megaureter underwent transperitoneal laparos...

Introduction: Intravesical recurrence after treatment of primary upper urinary tract urothelial carcinomas (UUT-UCs) is common. While intravesical instillation is widely used to prevent recurrence after transurethral resection for primary bladder tumors, there is still no consensus on the prophylactic capability of intravesical chemotherapy in pre...

Background/purpose: There is relatively little literature on prevalence of chronic kidney disease (CKD) prior to surgery in patients with upper urinary tract urothelial carcinoma (UTUC). We evaluated the prevalence and clinical associated factors of baseline CKD in patients with UTUC. Methods: There were 785 patients with a pathologic diagnosis...

The nucleosome binding protein 1 (HMGN5/NSBP1) is a member of the HMGN protein family and is highly expressed in several kinds of cancer. Nevertheless, the role of NSBP1 in clear cell renal cell carcinoma (ccRCC) remains unclear. This study aimed to confirm the oncogenic role of NSBP1 in ccRCC using in vitro and in vivo models and explore the mecha...

To discuss the pathological and clinical characteristics, treatments and prognosis of chromophobe renal cell carcinoma (CRCC). We developed a database that contained 1,870 patients who were diagnosed with renal cell carcinoma (RCC) and who underwent surgery in our hospital between 2002 and 2010. Afterward, the clinical and pathological data of 53 C...

Few studies have evaluated the growth pattern of renal cell carcinoma (RCC) in patients with delayed treatment. This report investigated the growth rate and stage progression of incidentally discovered RCC following a long period of active surveillance. Thirty-two patients who did not receive immediate surgical treatment for renal solid masses that...

To discuss the surgical skills and clinical value of complete transperitoneal laparoscopic nephroureterectomy. We collected and analyzed the clinical data of 25 patients (14 renal pelvic carcinoma and 11 carcinoma of ulreter, right side 15 and left side 10) who underwent complete transperitoneal laparoscopic nephroureterectomy for the upper urinary...

To investigate the effect of intermittent hormonal therapy (IHT) for patients with different stage/grade prostate cancer (PCa). The number of cycles and the duration of ON/OFF therapy for 45 PCa patients receiving IHT were observed. Maximal androgen blockade (MAB) therapies were used for six to nine months, and then stopped until the serum prostate...