Li Jingwei

Noethwest A&F university; Kyoto University

Sustainable production of pome fruit crops is dependent upon having virus-free planting materials. The production and distribution of plants derived from virus- and viroid-negative sources is necessary not only to control pome fruit viral diseases but also for sustainable breeding activities, as well as the safe movement of plant materials across b...

Lilium is one of the most popular flower crops worldwide, and some species are also used as vegetables and medicines. The availability of and easy access to diverse Lilium genetic resources are essential for plant genetic improvements. Cryopreservation is currently considered as an ideal means for the long-term preservation of plant germplasm. Over...

Production and maintenance of virus‐free planting materials is pivotal for the control of viral diseases. The present study attempted to test exogenous application of melatonin for eradication of apple stem grooving virus (ASGV) from the virus‐infected in vitro shoots of apple ‘Gala’. Exogenous application of 15 μM melatonin to the shoot proliferat...

Chrysanthemum stunt viroid (CSVd) and chrysanthemum chlorotic mottle viroid (CChMVd) are the two viroids most frequently infecting chrysanthemum. This study attempted to cryopreserve in vitro shoot tips of Chrysanthemum morifolium ‘Piato’ infected with high or low titers of CSVd, and co-infected with CSVd and CChMVd. By optimizing several key facto...

Availability of and easy access to diverse plant viruses and viroids is a prerequisite in applied and basic studies related to viruses and viroids. Long-term preservation of viruses and viroids is difficult. A protocol was described for long-term preservation of potato leafroll virus (PLRV), potato virus S (PVS), and potato spindle tuber viroid (PS...

Abstract Production of virus-free plants is necessary to control viral diseases, import novel cultivars from other countries, exchange breeding materials between countries or regions and preserve plant germplasm. In vitro techniques represent the most successful approaches for virus eradication. In vitro thermotherapy-based methods, including combi...

Background: Preservation of plant virus is a fundamental requirement in all types of virus-related research and applied applications. Development of efficient, reliable strategies for long-term preservation of plant virus would largely assist these studies. Results: The present study reported a novel biotechnology allowing cryopreservation of Ap...

Viral diseases (a biotic stress) and salinity (an abiotic stress) have been/are the two major constraints for sustainable development of the world’s agricultural production including potato. Crops grown in field are often exposed simultaneously to abiotic and biotic stress, and responses of plants to co-stress by two or more factors may differ from...

Globally, forests are of great economic importance and play a vital role in maintaining friendly ecological environments, sustainability of eco-systems, and biodiversity. Harsh environments, human activities and climate warming have long threatened the diversity of forest genetic resources. Among all conservation strategies, cryopreservation is at...

Buds of in vitro-grown shoots of two purple-fleshed potato genotypes were successfully cryopreserved by encapsulation-vitrification (Encap-vitri) and droplet-vitrification (Drop-vitri). Optimal time durations of exposure to PVS2 for shoot regrowth of cryopreserved buds were 5–7 h and 6 h for ‘E03-2677’ and for ‘Blue Congo’, respectively, in Encap-v...

Torenia fournieri, a popular potted flower crop, is frequently used as a model plant in genetic transformation studies, and transgenic plants expressing various foreign genes have been obtained in this plant. A safe and long-term conservation of the transgenic plants is necessary. In this study, shoot tips of transgenic plants of T. fournieri were...

Plant pathogen diseases have for a long time been a great threat to the sustainable development of horticulture. Use of disease-free planting materials is pivotal for the control of plant diseases. Cryotherapy, a novel biotechnology technique developed recently based on cryopreservation techniques, can efficiently eliminate plant pathogens, such as...

Plant cells undergo various stresses during cryopreservation procedures. Accumulation of reactive oxygen species (ROS) in plants is generally increased under stress conditions. Oxidative damage may subsequently affect recovery of cryopreserved samples. In the present study, we demonstrate that over-expression of the Arabidopsis thaliana glutathione...

China is now the largest potato producing country worldwide. The establishment of efficient conservation techniques for potato germplasm is a prerequisite for breeding of elite cultivars. Potato viral diseases have been a great threat for sustainable potato production in China. Use of virus-free seed tubers is an effective and practical means to co...

Effects of Potato leafroll virus (PLRV) and Potato virus Y (PVY) on vegetative growth, physiological metabolism and microtuber production were investigated using in vitro shoot cultures. The results showed that parameters of shoot growth including bud break percentage, shoot length, and node number and length were markedly reduced in the diseased s...

Horticultural crops are economically valuable for sustainable agricultural production. Plant diseases caused by Pathogens including virus, phytoplasma and bacterium have been a great threat to production of horticultural crops. The efficient use of pathogen-free plant materials has overcome the menace of plant diseases and has sustained crop produc...