About
51
Publications
9,166
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
1,064
Citations
Introduction
Skills and Expertise
Additional affiliations
August 2014 - December 2014
Publications
Publications (51)
Purpose
Limited research exists on the time course of long-term retinal and cerebral deficits in diabetic rodents. Previously, we examined short term (4–8 weeks) deficits in the Goto-Kakizaki (GK) rat model of Type II diabetes. Here, we investigated the long-term (1–8 months) temporal appearance of functional deficits (retinal, cognitive, and motor...
Pressure waves due to explosions or other traumatic events can damage the neurons of the eye and visual centers of the brain, leading to functional loss of vision. There are currently few treatments for such injuries that can be deployed rapidly to mitigate damage. Brain-derived neurotrophic factor (BDNF) and activation of its receptor tropomycin-r...
Purpose:
Exposure to high-intensity or outdoor lighting has been shown to decrease the severity of myopia in both human epidemiological studies and animal models. Currently, it is not fully understood how light interacts with visual signaling to impact myopia. Previous work performed in the mouse retina has demonstrated that functional rod photore...
Hyperspectral imaging (HSI) is an emerging new technology in solid tumor diagnosis and detection. It incorporates traditional imaging and spectroscopy together to obtain both spatial and spectral information from tissues simultaneously in a non-invasive manner. This imaging modality is based on the principle that different tissues inherit different...
- [...]
Alternative polyadenylation (APA) is a widespread and important mechanism in regulation of gene expression. Dysregulation of the 3' UTR cleavage and polyadenylation represents a common characteristic among many disease states including lung fibrosis. In this study, we investigated the role of mammalian cleavage factor I (CFIm)-mediated APA in regul...
Background
Long noncoding RNAs (lncRNAs) can act as microRNA (miRNA) sponges to regulate protein-coding gene expression; therefore, lncRNAs are considered a major part of the competitive endogenous RNA (ceRNA) network and have attracted growing attention. The present study explored the regulatory mechanisms and functional roles of lncRNAs as ceRNAs...
Single-cell sequencing technology refers to the sequencing of the genome, transcriptome and epigenome in one single cell. Comparing to traditional histology, single-cell sequencing can reveal the genetic heterogeneity among different cells. Due to the complex pathogenesis and various pathological types of hepatocellular carcinoma (HCC), studies on...
Vascular smooth muscle cells (VSMCs) are the predominant cell type in the arterial wall. These cells play a critical role in maintaining vascular homeostasis including vasoconstriction and vasodilatation through active contraction and relaxation. Dysregulation of VSMC function alters the response of blood vessels to mechanical stress, contributing...
Sepsis refers to a life-threatening organ dysfunction caused by a dysregulated host response to infection. Its pathogenesis is partly attributable to dysregulated inflammatory responses orchestrated by innate immune cells (e.g., macrophages and monocytes) that sequentially release early (e.g., TNF, IL-1, and IFN-γ) and late (e.g., HMGB1) pro-inflam...
Regulation of Vascular Smooth Muscle Cell Stiffness and Adhesion by [Ca2+]i: An Atomic Force Microscopy-Based Study – CORRIGENDUM - Volume 25 Issue 1 - Yi Zhu, Li He, Jing Qu, Yong Zhou
The intracellular concentration of calcium ion ([Ca ²⁺ ] i ) is a critical regulator of cell signaling and contractility of vascular smooth muscle cells (VSMCs). In this study, we employed an atomic force microscopy (AFM) nanoindentation-based approach to investigate the role of [Ca ²⁺ ] i in regulating the cortical elasticity of rat cremaster VSMC...
Significance
Circadian rhythms are not simply a passive consequence of cyclic fluctuations in the environment, but instead originate within the organism. Accumulating evidence indicates that disruption of circadian rhythms contributes to the development of many diseases. Here, we demonstrate that removal of the clock gene Bmal1 from the retina has...
Many types of retinal neuron modulate the distribution of their processes to ensure a uniform coverage of the retinal surface. Dendritic field area, for instance, is inversely related to the variation in cellular density for many cell types, observed either across retinal eccentricity or between different strains of mice that differ in cell number....
- [...]
α6-Integrin subunit (also known as CD49f) is a stemness signature that has been found on the plasma membrane of more than 30 stem cell populations. A growing body of studies have focused on the critical role of α6-containing integrins (α6β1 and α6β4) in the regulation of stem cell properties, lineage-specific differentiation, and niche interaction....
Hyperopic refractive error is detected by retinal neurons, which generate GO signals through a direct emmetropization signaling cascade: retinal pigment epithelium (RPE) into choroid and then into sclera, thereby increasing axial elongation. To examine signaling early in this cascade, we measured gene expression in the retina and RPE after short ex...
Hepatocellular carcinoma (HCC) is a common malignant tumor with high incidence and morbidity worldwide. The pathogenesis and progression of HCC are closely related to abnormal epigenetic regulation of hepatocytes. DNA methylation is an important regulatory mechanism in epigenetics which has been deeply and widely investigated. Along with the develo...
The stimulation of angiotensin II (Ang II), the effector peptide of renin–angiotensin system, has been reported to increase the expression of vascular endothelial growth factor (VEGF) through the activation of the Ang II type 1 receptor (AT1R). In this study, we investigated whether hyperglycemia (HG, 33 mM glucose) in ARPE-19 cells could promote t...
Assessing the incidence and severity of post-hepatectomy liver failure (PHLF) can be based on different criteria, and we wished to compare the diagnostic efficiency and specificity of different PHLF criteria. Data from patients (n=1683) who received hepatectomies in the liver surgery department of Peking Union Medical College Hospital from April 20...
Aggregated forms of α-synuclein play a crucial role in the pathogenesis of synucleinopathies such as Parkinson's disease (PD). However, the molecular mechanisms underlying the pathogenic effects of α-synuclein are not completely understood. Here we show that asparagine endopeptidase (AEP) cleaves human α-synuclein, triggers its aggregation and esca...
PURPOSE: MicroRNAs (miRNA/miR) are involved in a large number of biological functions and diseases such as cancer, cardiovascular diseases, and diabetes. MiR-21 has been reported to target Sprouty homolog (SPRY)1, SMAD7, and PTEN. In this study, we examined the underlying role of miR-21 in the regulation of Prorenin receptor (PRR)-mediated inductio...
Significance
We discovered that α-synuclein interacts with the neuroprotective protein phosphoinositide-3 kinase enhancer L (PIKE-L) in an S129 phosphorylation-dependent manner and sequesters PIKE-L in Lewy bodies, leading to the hyperactivation of AMP-activated protein kinase (AMPK) and subsequent dopaminergic neuronal cell death. Our findings may...
Purpose
To study the therapeutic effects of uterine artery embolization (UAE) on adenomyosis and to investigate the association between uterine blood supply and artery embolization treatment outcomes.
Methods
Using digital subtraction angiography (DSA) imaging data, we retrospectively evaluated the vascular features of 252 adenomyosis patients tre...
Purpose
The neuromodulator dopamine (DA) has been implicated in the prevention of excessive ocular elongation and myopia in various animal models. This study used retina-specific DA knockout mice to investigate the role of retinal DA in refractive development and susceptibility to experimental myopia.
Methods
Measurements of refractive error, corn...
The increased reactive oxygen species (ROS) caused by hyperglycemia (HG) is thought to be a key event in the pathogenesis of diabetic retinopathy. In diabetes, increased vascular endothelial growth factor (VEGF) expression is reported to increase ROS. Using a human RPE cell-line (ARPE-19), we investigated whether HG could promote the expression of...
Compelling evidences have suggested that high mobility group box-1 (HMGB1) gene plays a crucial role in cancer development and progression. This study aimed to evaluate the effects of single nucleotide polymorphisms (SNPs) in HMGB1 gene on the survival of gastric cancer (GC) patients. Three tag SNPs from HMGB1 gene were selected and genotyped using...
Circadian negative feedback loop (CNFL) genes play important roles in cancer development and progression. To evaluate the effects of single nucleotide polymorphisms (SNPs) in CNFL genes on the survival of GC patients, 13 functional SNPs from 5 CNFL genes were genotyped in a cohort of 1030 resected GC patients (704 in the training set, 326 in the va...
Objective:
A nucleosomal protein, HMGB1, can be secreted by activated immune cells or passively released by dying cells, thereby amplifying rigorous inflammatory responses. In this study we aimed to test the possibility that ionizing radiation similarly induces cytoplasmic HMGB1 translocation and extracellular release.
Method:
Human skin fibrobl...
Unlabelled:
Although the brain relies on auditory information to calibrate vocal behavior, the neural substrates of vocal learning remain unclear. Here we demonstrate that lesions of the dopaminergic inputs to a basal ganglia nucleus in a songbird species (Bengalese finches, Lonchura striata var. domestica) greatly reduced the magnitude of vocal l...
The increased reactive oxygen species (ROS) caused by hyperglycemia (HG) is thought to be a key event in the pathogenesis of diabetic retinopathy. In diabetes, increased vascular endothelial growth factor (VEGF) expression is reported to increase ROS. Using a human RPE cell-line (ARPE-19), we investigated whether HG could promote the expression of...
Sepsis, which refers to a systemic inflammatory response syndrome resulting from a microbial infection, represents the leading cause of death in intensive care units. The pathogenesis of sepsis remains poorly understood although it is attributable to dysregulated immune responses orchestrated by innate immune cells that are sequentially released ea...
High mobility group box 1 (HMGB1) is an evolutionarily conserved protein and is constitutively expressed in virtually all types of cells. In response to microbial infections, HMGB1 is secreted from activated immune cells to orchestrate rigorous inflammatory responses. Here we review the distinct mechanisms by which several herbal components inhibit...
Degeneration of retinal neurons is an underlying cause of several major types of blinding
diseases, and effective therapies remain to be developed. The suppositive strategy of repopulating
a degenerative retina with new cells generated onsite faces serious challenges,
because the mammalian retina seems to lack the ability to regenerate itself or re...
We examined gene expression in tree shrew choroid in response to three different myopiagenic conditions:
minus lens (ML) wear, form deprivation (FD), and continuous darkness (DK). Four groups of tree
shrews (n = 7 per group) were used. Starting 24 days after normal eye opening (days of visual experience
[DVE]), the ML group wore a monocular �5 D le...
Purpose:
We compared gene expression signatures in tree shrew sclera produced by three different visual conditions that all produce ocular elongation and myopia: minus-lens wear, form deprivation, and dark treatment.
Methods:
Six groups of tree shrews (n = 7 per group) were used. Starting 24 days after normal eye-opening (days of visual experien...
Better understanding of photoreceptor fate specification may lead to efficient production of photoreceptors for cell replacement studies. The authors investigated the role of proneural bHLH gene neurogenin1 (ngn1) in photoreceptor genesis using the chick retina.
In situ hybridization was used to delineate the spatial and temporal pattern of ngn1 ex...
Questions
Questions (2)
we bought dopamine and DOPAC standard for HPLC, DHBA 1ng/ml as internal control. When we loaded at the same amount, the peak of dopamine is 4 times higher than DOPAC. So in the standard/calibration curve, the coefficient or the slope of dopamine is much higher than DOPAC. Is this reasonable? Or same amount of DOPAC and dopamine should have similar peak and area in HPLC chromatograph?
Hello all, the experiment is like this:
Using real time PCR to test mRNA fold differences between treated eye and fellow control eye from the same animal, Animals are divided into 8 groups, each group (which has 6 animals) received one special treatment. Fold change comparison are made between treated eye and control eye in each animal in the same group, not across different groups. So it is like each treatment is a small seperate study, to see if the treatment has any effect on gene expression.
The question would be, can some of the treatments/groups use one reference gene, and other groups use a different reference gene? The reference gene is only used as a normalizer to detect the gene fold changes within the same group, not between different group.
Also, did anyone see papers which using one reference gene to test these conditions, and another reference gene to test other conditions?
Your ideas would be greatly appreciated.
