Leila Aparecida Salles Pio

Universidade Federal de Lavras (UFLA) | UFLA · Departamento de Agricultura

Pitaya production has been expanding in several regions of Brazil despite the lack of technical information on plant management. To obtain good crop productivity, weed control is essential; however, the use of herbicides can cause phytotoxicity and reduce plant growth and production. Thus, the present work aimed to evaluate the tolerance of red (Se...

The objective of this study was to (i) use a combination of a definitive screening design (DSD) and a complete 3³ factorial design to estimate the effects of four fertilizer supplements (factors) screened in the 1st planting cycle of Prata Gorutuba banana trees and (ii) determine whether combinations of these four factors can be identified for a fu...

Dragon fruit ( Selenicereus spp. syn. Hylocereus spp.) cultivation has grown significantly in recent years both in Brazil and throughout the world. This growth has demanded basic information on diseases caused by pathogens that can affect the crop. Among them, diseases of viral etiology stand out for the lack of curative control methods and for bei...

The demand for natural dyes of plant origin has increased in recent years. The peel of pitaya (dragon fruit—Hylocereus polyrhizus) is a food by-product that has high concentrations of betalains and, therefore, can be a source of extraction of this pigment. Because they are very unstable, the association of betalains with protective materials tends...

Choosing the appropriate management system is essential for sustainable agricultural practices. Yet, soil-specific properties at the subsurface are seldom considered when choosing the appropriate tillage system. This study assessed the effect of tillage depth on physical–hydraulic properties in three contrasting soil classes in the establishment of...

Abstract To produce pitaya (Hylocereus spp.), it is essential that pollination occur, either artificially or by pollinating agents. This study evaluated the viability of self-pollination, cross-pollination, and natural pollination, as well as pollen viability, stigma receptivity and ideal pollination window in pitaya flowers (Hylocereus spp.). An e...

Tillage modifies soil structure, which can be demonstrated by changes in the soil’s physical properties, such as penetration resistance (PR) and soil electrical resistivity (ρ). The aim of this study was to evaluate the effect of deep tillage strategies on three morphogenetically contrasting soil classes in the establishment of perennial crops rega...

Many important crops have cultivars with different ploidy and provide a diverse choice of materials for breeding programs. Therefore, it is essential to accurately assess the ploidy of all breeding materials. Increased DNA content is known to have various effects on external and internal morphology, an effect known as the “nucleotypic effect”. Thus...

Eucalyptus spp. it is known to be part of most forests planted for commercial use in Brazil, however when intercropped with Pitaia (Hylocereus undatus), few studies report this interaction. The objective of this work was to evaluate the effects of shading and the existence of allelopathic interaction between Eucalyptus spp and pitaia (H. undatus) i...

Brazilian banana farming is one of the most important agricultural activities in the national scenario and can be highly productive and economically profitable for producers who invest in technology. The objective of this work was to compare the economic viability of two different fertilization managements in the cultivation system of 1st -cycle ‘P...

An increase in crop productivity should be linked to the effective control of production costs. Thus, the chemical and selective control of weeds is an appropriate method because it saves labor and energy, requires little manpower, and allows control throughout the crop cycle. The present study aimed to evaluate the sensitivity of avocado seedlings...

The purpose of this paper is to physical-chemical characterize of male inflorescence of Prata-Anã and Grande Naine cultivars, and their respective flours. Three drying methods were used to prepare the flours: F1- drying in a forced-air oven at 40°C/30 hours; F2- at 60°C/24 hours and F3- conventional oven at 120°C/45 minutes. The banana tree male in...

Banana is among the world´s most popular fruits and it is consolidated in the international market. The largest abiotic constraint threatening banana production is water stress, in rainfed area, judicious use of water is essential for increasing the area under crop production with inadequate water supply. Under such situation the uses of moisture c...

Para se produzir pitaia é essencial que ocorra a polinização de suas flores, que pode ser realizada de forma artificial ou por agentes polinizadores. A polinização cruzada efetuada por insetos apresenta como vantagens o maior número de sementes, maior massa de frutos, vida de prateleira, maior valor nutritivo e o incremento nas taxas de frutificaçã...

The objective of this work was to evaluate the induction of indirect organogenesis by concentrations of dichlorophenoxyacetic acid (2,4-D) and thidiazuron (TDZ) in pitaya (Hylocereus undatus) explants, using scanning electron microscopy and the flow cytometry technique. The treatments consisted of the concentrations of 0, 2.0, and 4.0 mg L-1 2,4-D...

The objective of this work was to evaluate the viability of the micrografting of yellow dragon fruit (Selenicereus megalanthus) on different rootstocks, based on DNA content and anatomical analyses. The used rootstocks were: yellow dragon fruit, white dragon fruit (Hylocereus undatus), Saborosa (Selenicereus setaceus) dragon fruit, and the Cebra an...

Crop residues can be used in several ways, e.g processed and marketed for human consumption. The purpose of this paper is an initial approach to this few explored subject, testing two methodology of canning banana tree palm heart, treatment 1 (1% citric acid and 5% sodium chloride at the waiting liquid) and treatment 2 (200 ppm sodium bisulfite at...

A pitaia apresenta grande potencial de mercado e processamento. Porém, a falta de conhecimento sobre seu cultivo ainda é uma barreira que impede sua difusão no país. Assim, objetivou-se avaliar as alterações físico-químicas dos frutos de pitaia (Hylocereus undatus) colhidos em diferentes estágios de desenvolvimento bem como determinar o período ide...

The objective of this study was to evaluate the quality of white-fleshed dragon fruit (Hylocereus undatus) at harvest and postharvest to determine fruit quality and the feasibility of harvesting the fruits at different outer fruit color stages. The treatments consisted of four peel color stages – S1 (<25% red peel), S2 (25%-49% red peel), S3 (50% t...

Dragon fruit shows good sensory and nutritional attributes, but it is also highly perishable. Drying is a unitary operation that promotes the reduction of moisture content, extending the useful life of the product. In this study, the elaboration of white and red dragon fruit powder by foam mat drying was studied with optimization of the proportion...

Banana (Musa spp.) is a crop with outstanding economic and social expression throughout the world. The objective of this study was to investigate the effect of different mulches types in growth production and postharvest of banana ‘BRS Platina’ not irrigated. The experiment was conducted in the farm of the Federal University of Lavras (UFLA). Micro...

Given the high demand for food worldwide, options for maximizing food production with minimum environmental impact are needed. Therefore, the objective of the present study was to evaluate the effect of silicon (Si) and methyl jasmonate (MeJA) in the vegetative development and genetic stability of rice plants, BRSMG Caravera cultivar. The treatment...

Dekopon or Hallabong (Citrus reticulate “Shiranui”) is a hybrid fruit that belongs to the citrus fruits. The scientific and commercial interests in dekopon is due to its nutritional composition. The objective of the study was to verify the influence of ethanol as a pretreatment in reducing drying time as well as maintaining nutritional quality (vit...

Banana cultivation is an agricultural activity practiced in different regions and constantly subjected to abiotic stresses that limit its productivity. To treat the effect of these stresses research can be undertake by simulating them in vitro since it limit the effect of the external factor in the experiment. Therefore, the objective of this study...

Butia capitata (Mart.) Becc., commonly known as jelly palm, is a palm tree native to the Cerrado biome. It is considered an endangered species owing to intensive extractive exploitation of its fruits, with slow and uneven seminiferous propagation as an aggravating factor. Thereby, there are some bottlenecks related to jelly palm seedling production...

The objective of this work was to evaluate the effect of silicon fertilization on gas exchange, leaf anatomy, and ultrastructural characteristics of passion fruit (Passiflora edulis). The treatments comprised four concentrations of silicon (0, 0.28, 0.55, and 0.83 g per pot) at 1% silicic acid solution (SiO2.XH2O). This solution was applied around...

The effect of physical (fast freezing with liquid nitrogen and slow freezing followed by thawing) and chemical (alkaline solution of ethyl oleate) pretreatments on the kinetics and quality parameters of cape gooseberry (Physalis peruviana) during convective drying (60 C, 2 m/s) was studied. The influence of the pretreatments on the drying kinetics,...

Habitat fragmentation has numerous consequences, particularly to endemic species, and has a negative impact on the genetic diversity of neglected species, leading to genetic drift. Annona crassiflora Mart. is a species that is endemic to Brazil, and its incidence in the Cerrado biome has decreased. The identification and characterization of its rem...

RESUMO A pitaia é uma cactácea frutífera originária das florestas tropicais da América do Sul e Central. Produz frutos não convencionais muito apreciados, com grande potencial para diversificação do setor. É propagada preferencialmente por estaquia, proporcionando aumento na precocidade e uniformidade dos pomares. Com o intuito de maximizar a produ...

Curcuma longa L., from the Zingiberaceae family, generally reproduces through its rhizomes, which are also utilized for therapeutic purposes because they are rich in terpenoids. Its conventional propagation has low efficiency due to the small number of seedlings and their contamination by pathogens. Therefore, this study aimed to evaluate the influ...

Endoreduplication is the change of cellular cycle that result DNA duplication without cell division and could result endopolyploid cells. This phenomenon is common in plants and animals and considered as evaluative strategy. Although endoreduplication reported in various plant species, the information about these phenomena in red pitaya is rare. Th...

The red pitaya with white pulp (Hilocereus undatus) is a cactus belonging to the group of tropical fruit, considered promising for cultivation. This study aims to induce callus and embryogenic potential and determine the genetic stability in pitaya. The study was divided into four steps: 1a) Callus induction using doses of 2,4-D (2.0 and 4.0 mg L-1...

A determinação do nível de ploidia é muito importante, principalmente em programas de melhoramento genético que envolvem poliploides, a fim de possibilitar a escolha adequada dos materiais vegetais com os quais se deseja trabalhar. A relação entre o conteúdo de DNA de acessos de bananeira e sua ploidia ainda permanece controversa na literatura; ass...

We determined the DNA contents and assessed the quality of the flow cytometry analyses of different structures of Butia capitata arising at various stages during the In vitro germination and growth of zygotene embryos, characterizing them both morphologically and anatomically. The experiments evaluated 16 organs and stages, with the embryo, cotyled...

This study aimed to induce callus formation in Jatropha curcas L. and to evaluate the ultrastructure and cytochemical behavior of the calli. Calluses were induced with 2,4-D, picloram-PIC, kinetin (Kin) and BAP: (1) control; (2) 4.52 μM 2,4-D; (3) 9.04 μM 2,4-D; (4) 4.14 μM PIC; (5) 8.28 μM PIC; (6) 4.52 μM 2,4-D + 2.32 μM KIN; (7) 9.04 μM 2,4-D +...

The aim of this study was to evaluate the reliability of flow cytometry analysis and the use of this technique to differentiate species and varieties of sugarcane (Saccharum spp) according to their relative DNA content. We analyzed 16 varieties and three species belonging to this genus. To determine a reliable protocol, we evaluated three extractio...

In the present work, polyploidy was induced in the diploid banana varieties 'Malbut', 'Gold', 'Lidi', and 'Thong Dok Mak' through the use of colchicine and oryzalin, and that condition was identified through stomatal analysis, flow cytometry, and chromosome counts. Shoots produced in vitro were treated with colchicine at concentrations of 0, 2.5, 7...

This study aimed to evaluate the effect of spectral light quality on different anatomical features of banana tree plantlets grown under coloured shade nets. Banana plants of five genotypes (Maçã, Thap Maeo, Caipira, BRS Platina and Princesa), obtained from micropropagation, were grown under white, blue, red and black nets, with shade of 50%, in a c...

Flow cytometry is a technique that yields rapid results in analyses of cell properties such as volume, morphological complexity and quantitative DNA content, and it is considered more convenient than other techniques. However, the analysis usually generates histograms marked by variations that can be produced by many factors, including differences...

In vitro conservation techniques can be utilized for germplasm maintenance. However, few reports on the in vitro conservation of sugarcane species are present in the literature. The objective of this study was to subject sugarcane plants to in vitro under minimal growth conditions and to evaluate the survival, regeneration, and the monitoring of nu...

The objective was to develop a methodology to enable the classification of banana crop subjected to induction of chromosome doubling using Neural Networks (NN). The data used in this study were taken from a thesis already presented, whose authors studied the correlation between fresh weight of leaf discs and DNA content. The NN was implemented by t...

Pesquisas acerca das modificacoes estruturais e fisiologicas inerentes ao processo de aclimatizacao sao fundamentais para compreender esta fase. Assim, o objetivo deste trabalho foi avaliar diferentes fontes de silicio no cultivo in vitro de bananeira ‘Grande Naine’ sobre caracteristicas bioquimicas, fitotecnicas e ultraestruturais, durante o proce...

Micropropagation makes the obtaining of seedlings of red pitaya easier, but, it can cause genetic mutations such as polysomaty, endopolyploidy or endoreduplication. In this work, it was aimed to develop a protocol for micropropagation and check the endoreduplication in red pitaya. Pitaya cladodes were micropropagated im MS culture medium with 0.1 m...

Flow cytometry and microsatellite analyses were used to evaluate the trueness-to-type of somatic embryogenesis-regenerated plants from six important Spanish grapevine (Vitis vinifera L.) cultivars. Tetraploid plants were regenerated through somatic embryogenesis from all of the cultivars tested with the exception of ‘Merenzao’. In addition, an octo...

In vitro plant culture under conventional heterotrophic conditions promotes the development of certain undesirable morpho-physiological characteristics, such as reduced epicuticular wax deposition and mesophyll differentiation, rudimentary vascular bundles and little control of stomata opening and closing. Silicon is a beneficial element for plants...

The objective of the research was to evaluate the effect of different sources of silicon during the in vitro rooting phase of 'Maçã' banana tree by means of physiological, phytotechnical and ultrastructural analysis. It was used propagules already established in vitro which were inoculated in MURASHIGE & SKOOG (MS) medium, added of 30g L-1 of sucro...

The aim of this study was to determine morphological descriptors that could enable the characterization of banana cultivars from micropropagation process during the juvenile phase. Twelve cultivars from different genomic groups and with different ploidy levels were grown in vitro and acclimatized in greenhouse for three months. After this period, p...

O elevado custo do nitrato de amônio, aliado à dificuldade de aquisição do mesmo, tem levado à realização de trabalhos, no sentido de buscar alternativas para a substituição dessa fonte de nitrogênio. Objetivou-se estudar a viabilidade técnica da substituição do nitrato de amônio por uréia, como fonte de nitrogênio no meio de cultura para o cultivo...

The high cost allied to the difficulty in the acquisition of the ammonium nitrate has been taking the accomplishment of works looking for an alternative for the substitution of this source of nitrogen. It was aimed at to study the technical viability of the substitution of the ammonium nitrate for urea, as source of nitrogen in the culture media fo...

Annona crassiflora Mart known as 'araticum', 'marolo' or 'field araticum' is a typical fruit from the Cerrado biome of Brazil with socio-economic and medicinal importance. Normally, Annona crassiflora is propagated through seeds. However, due to a deep dormancy that the seeds display at dispersion and the difficulty to obtain uniform plants in a sh...

This work was developed with the aim of improving technics of in vitro propagation of blackberry. So, one tested in an experiment the influence of five different ANA concentrations (0; 0,1; 0,5; 1,0 and 1,5 mg L-1) and five AG3 (0; 2,0; 4,0; 6,0 and 8,0 mg L-1), added to the culture medium MS, to the blackberry cv. Ebano and; in a second experiment...

The blackberry (Rubus sp.) micropropagation is used, mainly for obtaining virus-free plants in short period of time. In the present work different concentrations of sodium chloride (NaCl) and naphthaleneacetic acid (NAA) were tested added to in vitro culture medium of blackberry cv. Brazos. The culture medium consisted of MS salts, with 30 g L-1 su...

Aiming to improve the in vitro cultivation techniques of two temperate fruit i.e. blackberry cv. Tupy and grapevine rootstock cv. Kobber 5BB, different glycine and inositol concentrations in the culture medium were tested. The culture medium was constituted of MS basal medium, added of 30 g L-1 sucrose and 7 g L-1 agar, and the pH adjusted to 5.8 b...

This experiment was carried out on a ten year-old commercial orchard in São João Del Rei county–MG in order to extend the harvest season of tangerine Ponkan (Citrus reticulate Blanco). One evaluated the effects of Giberelic Acid (GA3) at 0, 10, 20 and 30 mg.0L-1 and Diclorofenoxiacetic acid (2,4- D) at 0 and 10 mg.L-1 applied two times: on 4/24 and...

This experiment was carried out on a ten year-old commercial orchard in São João Del Rei county MG in order to extend the harvest season of tangerine Ponkan (Citrus reticulate Blanco). One evaluated the effects of Giberelic Acid (GA3) at 0, 10, 20 and 30 mg.0L-1 and Diclorofenoxiacetic acid (2,4-D) at 0 and 10 mg.L-1 applied two times: on 4/24 and...

The micropropagation of blackberry can generate virus-free plants in short time. In order to improve micropropagation techniques of blackberry cv. Tupy (Rubus spp.), nodal segments with 2 cm length and 2 axillary buds originating from plants in vitro were excised and inoculated in MS culture medium, supplemented with different concentrations of sod...

Paracoccidioides brasiliensis, the etiologic agent of paracoccidioidomycosis, is a facultative intracellular human pathogen that can persist within macrophage phagolysosomes, indicating that the fungus has evolved defense mechanisms in order to survive under nutritionally poor environments. The analysis of P. brasiliensis transcriptome revealed sev...

The present work was accomplished to evaluate the effects of storage on the viability of pollen grains from Natal, Pêra and Valência cultivars of sweet oranges. The grains were stored at 3 temperatures: -10ºC (freezer), 4ºC (refrigerator), and room temperature; 2 environments (with or without desiccator) and in the presence or absence of silica-gel...

O presente trabalho foi realizado visando avaliar a viabilidade de grãos de pólen armazenados das cultivares copas cítricas Natal, Pêra e Valência. O pólen foi submetido a 3 temperaturas de armazenamento: -10ºC (freezer), 4ºC ( refrigerador) e temperatura ambiente; 2 ambientes (com e sem dessecador) e na presença e ausência de sílica-gel. A avaliaç...

A micropropagacao da videira pode gerar plantas livres de virus e em curto espaco de tempo. Com o objetivo de aprimorar tecnicas de propagacao in vitro, testaram-se concentracoes de meio de cultura MS e de sacarose. Gemas axilares com 2,0 cm, de plantulas preestabelecidas in vitro do porta-enxerto de videira 043-43, foram excisadas em condicoes ass...

A micropropagação da amoreira-preta pode gerar plantas livres de vírus e em curto espaço de tempo. Com o objetivo de aprimorar técnicas de micropropagação de amoreira-preta cultivar Brazos (Rubus idaeus L.), segmentos nodais, oriundos de plântulas preestabelecidas in vitro foram excisados e inoculados em meio WPM (0, 50, 100, 150 e 200%), suplement...

In vitro multiplication of rootstock grapevine in variations of culture media. The grapevine micropropagation can generate virus-free plants and in short time period. Aiming at improving in vitro propagation techniques, concentrations of MS culture medium and sucrose were tested. Axillary buds (2.0 cm length) of '043-43' rootstocks maintained in vi...

O objetivo do presente estudo foi aprimorar técnicas de propagação in vitro e determinar um melhor substrato para a amoreira-preta. O primeiro experimento consistiu de gemas axilares com cerca de 2 cm, oriundas de plântulas preestabelecidas in vitro da cv. Ébano, excisadas e inoculadas em meio MS, suplementado com 5 concentrações de carvão ativado...

O objetivo do presente estudo foi aprimorar técnicas de propagação in vitro e determinar um melhor substrato para a amoreira-preta. O primeiro experimento consistiu de gemas axilares com cerca de 2 cm, oriundas de plântulas preestabelecidas in vitro da cv. Ébano, excisadas e inoculadas em meio MS, suplementado com 5 concentrações de carvão ativado...

Activated charcoal and gibberelin provides better conditions on development of citrus immature embryos. Activated charcoal and GA3 (gibberelic acid) on 'Pêra Rio' sweet orange x 'Poncã' mandarin immature embryos culture was evaluated. After 118 days-pollination, imature fruits with 3 to 4 cm of diameter were collected, seeds removed and treated wit...

With the objective to micropropagation the blackberry (Rubus sp.), cv. Ébano, nodal segments of plants in vitro, originated from Embrapa Clima Temperado/CPACT-Pelotas, RS, were inoculated in MS culture medium (0, 50, 100, 150 and 200%), suplemented with different BAP concentrations (0; 0,5; 1,0; 2,0 and 4,0 mg L-1). The pH was adjusted for 5,8 befo...

The study was carried out in order to evaluate the effect of temperature and in vitor stigma receptivity on regeneration of orange cultivar (Valencia, Pera and Natal) pollen. Two experiments were carried out, in the first the ideal temperature of germination was assessed. Pollen was obtained from flowers at the balloon stage and inoculated in cultu...

Dissertação (Mestrado)--Universidade Federal de Lavras.