About
42
Publications
20,502
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
1,088
Citations
Introduction
Additional affiliations
October 2019 - present
June 2019 - October 2019
January 2019 - June 2019
Education
September 2011 - November 2014
January 2010 - October 2010
September 2002 - June 2008
Publications
Publications (42)
The stem cell field has grown very rapidly during the last decade, offering the promise of innovative therapies to treat disease. Different stem cell populations have been isolated from various human adult tissues, mainly from bone marrow and adipose tissue, but many other body tissues harbor a stem cell population. Adult tissue stem cells are inva...
It has been described that adult tissues contain mesenchymal stem cell populations. The specific areas where stem cells reside are known as niches. Crosstalk between cells and their niche is essential to maintain the correct functionality of stem cell. MSCs present a set of abilities such as migration, invasion, and angiogenic potentials, which mak...
Background:
The role of adipose tissue in the pathophysiology of cardiovascular disease remains a major subject of research. The objective of the present study was to dissect the molecular mechanisms that regulate the survival and differentiation of cardiac cells in an obese environment.
Material and methods:
We isolated murine/human cardiac cel...
Pericardial adipose tissue (PAT), a visceral fat depot enveloping the heart, is an active endocrine organ and a source of free fatty acids and inflammatory cytokines. As in other fat adult tissues, PAT contains a population of adipose stem cells; however, whether these cells and/or their environment play a role in physiopathology is unknown. We ana...
The polyphenol-enriched extracts (PEEs) of Chilean currants Ribes magellanicum and R. punctatum were submitted to in vitro gastro-intestinal digestion and their anti-inflammatory activities were assessed using differentiated human Caco-2 (clone C2BBe1) cells stimulated with interleukin 1β (IL-1 β). The inhibitory effect of non-digested and digested...
Ultrasound is considered a safe and non-invasive tool in regenerative medicine and has been used in the clinic for more than twenty years for applications in bone healing after the approval of the Exogen device, also known as low-intensity pulsed ultrasound (LIPUS). Beyond its effects on bone health, LIPUS has also been investigated for wound heali...
Background
Mesenchymal stem cells (MSCs) activate the endogenous immune regulatory system, inducing a therapeutic effect in recipients. MSCs have demonstrated the ability to modulate the differentiation of myeloid cells toward a phagocytic and anti-inflammatory profile. Allogeneic, adipose-derived MSCs (ASCs) have been investigated for the manageme...
Ultrasound has emerged as a novel tool for clinical applications, particularly in the context of regenerative medicine. Due to its unique physico-mechanical properties, low-intensity ultrasound (LIUS) has been approved for accelerated fracture healing and for the treatment of established non-union, but its utility has extended beyond tissue enginee...
A common null polymorphism (rs1815739; R577X) in the gene that codes for α-actinin-3 (ACTN3) has been related to different aspects of exercise performance. Individuals who are homozygous for the X allele are unable to express the α-actinin-3 protein in the muscle as opposed to those with the RX or RR genotype. α-actinin-3 deficiency in the muscle d...
A common null polymorphism in the ACTN3 gene (rs1815739:C>T) results in replacement of an arginine (R) with a premature stop codon (X) at amino acid 577 in the fast muscle protein α‐actinin‐3. The ACTN3 p.Arg577Ter allele (aka p.R577* or R577X) has undergone positive selection, with an increase in the X allele frequency as modern humans migrated ou...
Cell migration is an essential process throughout the life of vertebrates, beginning during embryonic development and continuing throughout adulthood. Stem cells have an inherent ability to migrate, that is as important as their capacity for self-renewal and differentiation, enabling them to maintain tissue homoeostasis and mediate repair and regen...
Adipose tissue is a significant source of mesenchymal stem cells for regenerative therapies; however, caution should be taken as their environmental niche can affect their functional properties. We have previously demonstrated the negative impact of obesity on the function of adipose-derived stem cells (ASCs). Here we have evaluated other possible...
A wealth of evidence supports an association between regular physical activity (PA) and decreased risk for cancer and cancer mortality. This is clearly an important issue given the growing worldwide incidence of both cancer and physical inactivity. Of further importance is unraveling the biological mechanisms that explain the potential preventive e...
Obesity affects the functional capability of adipose-derived stem cells (ASCs) and their effective use in regenerative medicine through mechanisms still poorly understood. Here we employed a multiplatform (LC/MS, CE/MS, GC/MS) metabolomics untargeted approach to investigate the metabolic alteration underlying the inequalities observed in obese-deri...
Introduction:
We recently generated a knock-in mouse model (PYGM p.R50X/p.R50X) of McArdle disease (myophosphorylase deficiency). One mechanistic approach to unveil the molecular alterations caused by myophosphorylase deficiency, which is arguably the paradigm of 'exercise intolerance', is to compare the skeletal-muscle tissue of McArdle, heterozy...
Mesenchymal precursors (MPs) present some advantageous features, such as differentiation and migration, which make them promising candidates for cell therapy. A better understanding of MP migration characteristics would aid the development of cell delivery protocols. Traditionally, cell migration is thought to occur only through the formation of la...
The increasing aging of our societies is accompanied by a pandemic of obesity and related cardiometabolic disorders. Progressive dysfunction of the white adipose tissue is increasingly recognized as an important hallmark of the aging process which in turn contributes to metabolic alterations, multi-organ damage, and a systemic pro-inflammatory stat...
Concentration of blebbistatin assay.
Representative images (20x) of MPs when blebbistatin was added at different concentrations (30ng/ml, 600 ng/ml and 3000 ng/ml) upon seeding of cells. Images were taken 24 hours after cell seeding.
(PDF)
Muscle precursors in culture.
Muscle precursors (40x) 24 hours after seeding, without any coating present many blebs can be seen for an extended time while the cell was changing greatly in morphology. Images were taken every 5 seconds and were stitched together with a one second gap.
(MOV)
Migration of adipose precursors.
Blebs can be seen in the leading edge of adipose precursors (20x), 24 hours after seeding, without any coating. Images were taken every 5 minutes during 2 hours and were stitched together with a gap of 0.4 seconds.
(MOV)
Blebs in MPs.
Representative images of different MPs with rounded or elongated shape (magnification of 40x).
(PDF)
pMLC staining in MPs.
Images (63x and magnification) of pMLC and F-actin staining of cells in control and blebbistatin conditions (added after 24h of seeding the cells).
(PDF)
Blebs and their migration capacity.
Graph showing the relationship between the presence of blebs in each precursor and their capacity of migration.
(PDF)
Cardiac precursors in culture.
Cells (40x), 6 hours after seeding without any coating, show large and very dynamic blebs around the plasma membrane. Images were taken every 5 seconds and were stitched together with a one second gap.
(MOV)
High migration capacity of muscle precursors.
Muscle precursors (20x) have many dynamic blebs in the leading edge. 24 hours after seeding, without any coating. Images were taken every 5 minutes during 2 hours and were stitched together with a gap of 0.4 seconds.
(MOV)
Culture of MPs with/without blebbistatin.
(A) Representative images (20x) of MPs when blebbistatin was added upon seeding of cells. Graph showing percentages of cells with blebs, data obtained from the images taken at 6h; * P < 0.05. (B) Images of MPs (20x) when blebbistatin was added after 24h of culture. Graph showing percentages of cells with bl...
Adipose precursors in culture.
These precursors (40x) show dynamic blebs in the leading edge of elongated cells 24 hours after seeding, without any coating. Images were taken every 5 seconds and were stitched together with a one second gap.
(MOV)
Migration of cardiac precursors, Cardiac precursors (20x) show blebs 24 hours after seeding, without any coating.
Images were taken every 5 minutes during 2 hours and were stitched together with a gap of 0.4 seconds.
(MOV)
Objective:
The therapeutic potential of adipose-derived stem cells (ASCs) is reduced by various stress-inducing conditions that affect tissue homeostasis such as diabetes, aging, and obesity. Previous works have provided evidence of negative effects of obesity on ASC populations, but it is unclear whether this persists after a weight loss. This st...
Although low- to moderate-intensity exercise has well-known cardiovascular benefits, it has been increasingly suggested that prolonged strenuous endurance exercise (SEE) could have potential deleterious cardiac effects. In effect, the term 'cardiac overuse injury' (or 'over-exercise') has been recently reported to group all the possible deleterious...
Cell-based therapy is a promising approach for many diseases, including ischemic heart disease. Cardiac mesoangioblasts are committed vessel-associated progenitors that can restore to a significant, although partial, extent, heart structure and function in a murine model of myocardial infarction. Low-intensity pulsed ultrasound (LIPUS) is a non-inv...
Inflammation is a double-edged sword with both detrimental and beneficial consequences. Understanding of the mechanisms of crosstalk between the inflammatory milieu and human adult mesenchymal stem cells is an important basis for clinical efforts. Here, we investigate changes in the transcriptional response of human adipose-derived stem cells to ph...
Adipose stem cells (ASCs) are an appealing source of cells for therapeutic intervention; however, the environment from which ASCs are isolated may impact their usefulness. Using a range of functional assays, we have evaluated whether ASCs isolated from an obese environment are comparable to cells from non-obese adipose tissue. Results showed that A...
Abstract Efficient delivery of stem cells to target tissues is a major problem in regenerative medicine. Adipose derived stem cells have been proposed as important tools in cell therapy for recovering tissues after damage. Nevertheless, the ability of these ASCs to migrate or invade in order to reach the tissue of interest has not been tested so fa...
Adipose-derived stem cells (ASCs) are promising candidates for autologous cell-based regeneration therapies by virtue of their multilineage differentiation potential and immunogenicity, however relatively little is known about their role in adipose tissue physiology and dysfunction. Here we evaluated whether ASCs isolated from non-obese and obese t...
The present study reports an easy and efficient method for obtaining adult mesenchymal precursors from different adult mouse tissues.
We describe the isolation and expansion of mesenchymal precursors from skin and lung by a non-enzymatic method. Skin and lung mesenchymal precursors isolated by a modified explant technique were characterized in vitr...
Differentiation properties of MPCs. Percentages of skin and lung MPCs clones that differentiate into adipose, chondrogenic, osteogenic or muscle tissues in three independent experiments (Student T-test; *p<0.01).
(TIF)
