L. Rajanna

Bangalore University | BU · Department of Botany

Mansoa alliacea (Lam.) A. H. Gentry is a woody vine belonging to Bignoniaceae. The present study evaluated the antincancerous efficacy of M. alliacea leaf extracts against Human epidermoid carcinoma cell line A-431. Leaf extracts were prepared using different solvents viz., petroleum ether, chloroform, ethyl acetate, ethanol and water. Cell viabili...

The current study was aimed at obtaining the phytochemical profile of leaf extracts of Aristolochia tagala Cham., a rare ethnomedicinal plant by GC-MS analysis. The chromatograms of GC-MS analysis of methanol, chloroform, and petroleum ether extracts of leaf showed 39, 37, and 30 peaks respectively. The mass of compounds and fragments recorded were...

Aristolochia tagala Cham. is a rare medicinal plant found in certain tropical regions of the world. During the present investigation, a detailed preliminary qualitative and quantitative analysis were carried out to know the phytochemical profile of the leaves of A. tagala. The leaf powder was extracted with three solvents having different polaritie...

Aristolochia tagala Cham. is an important medicinal plant used to treat tumors in ethno botanical Practices. During the present investigation, dried leaf powder of A. tagala was extracted with three different solvents by decoction method and were subjected to MTT cytotoxicity assay against HepG2 – human liver carcinoma cell line. Methanolic leaf ex...

Canthium dicoccum is a shrub belongs to the family Rubiaceae. Ethnomedicine has evident the medicinal properties of different parts of this plant. During the present investigation, qualitative phytochemical analysis was carried out on the leaf extract of C. dicoccum and the results revealed the presence of carbohydrates, glycosides, saponins, alkal...

The aim of the present study was to assess and evaluate the therapeutic value of fruit stalk of Capsicum annuum var. glabriusculum (Dunal) Heiser & Pickersgill found across homegardens of Uttar Kannada. The study involved the screening of phytochemical (qualitative) and quantitative estimation of phenolic content, flavonoid content and to assess th...

Presence of certain chemical compounds is responsible for medicinal properties of the plant. In the present study, the various biochemical tests were conducted to examine the presence of various phytochemicals in the methanolic, petroleum ether and chloroform tuber extracts of Ruellia tuberosa L. Also, the extracts were subjected to the in vitro ha...

Aim: Aristolochia indica Linn. is a medicinal plant used to cure various forms of bowel complaints, fever and skin disease. Hence in the present study this plant was tested for its antimicrobial efficacy against the microbes causing such diseases. Method: Three solvent (methanol, petroleum ether and chloroform) extracts of leaf of Aristolochia indi...

Tissue cultured Pimpinella tirupatiensis plantlets were subjected to assessment of genetic stability considering the fact that associated in vitro stress might result in breakdown of control mechanism causing instability of the genome. We have used two DNA based molecular markers to assess the genetic fidelity of in vitro regenerated Pimpinella tir...

An efficient micropropagation protocol has been standardized for the shoot regeneration from the nodal explants of Vitex agnus-castus, an important aromatic medicinal plant. Nodal segments were cultured on MS medium supplemented with various concentrations of plant growth regulators. Among them, MS + 2.5 mg/l IBA or MS + 3.0 mg/l IBA and MS + 2.0 m...

The present study was conducted in a semi arid region of Karnataka. The study discusses the phytoplankton diversity of the Gogi lake ecosystem. A standard methodology was followed in conducting to complete this study and samples were collected at different points from the lake ecosystem located at the core area of the proposed uranium mining site....

An efficient protocol for axillary bud proliferation and direct organogenesis has been developed in Aristolochia tagala Cham. through in vitro propagation technique. Nodal segments and shoot tips were cultured on Murashige and Skoog's media (MS media) supplemented with various concentrations of growth hormones. MS media fortified with BAP 1 mg/L an...

The flavonoid fractions I, II and III isolated from the leaf extract of Baliospermum montanum, Drypetes roxburghii and Codiaeum variegatum possess significant antiinflammatory and cytotoxic activity against mouse leukaemic monocyte macrophage cell line RAW 264.7. In B. montanum, the cytotoxic activity of LPS-stimulated mouse macrophages (RAW 264.7...

Vitex agnus-castus L (VAC) is an important medicinal plant. Seeds are used as a popular treatment for the management of female reproductive system disorders. Other uses include the treatment of hangovers, flatulence, fevers, benign prostatic hyperplasia, nervousness, dementia, rheumatic conditions, cold, dyspepsia, spleen disorders, constipation an...

The present study reveals the qualitative and quantitative analysis of the major bioactive constituents of different parts such as root, stem, leaf, flower and latex of medicinally important plant Baliospermum montanum using seven different solvents. Qualitative study of Alkaloids, Carbohydrates, Glycosides, Steroids, Flavonoids, Coumarins, Saponin...

The aim of DNA barcoding is to establish a shared community resource of DNA sequences that can be used for identification, discrimination or taxonomic classification of organisms. It is a method of choice that uses a short genetic marker in an organism's DNA to identify and distinguish its belonging from particular species, varieties or even inter...

Histochemical studies of the haustorium of Cuscuta reflexa and Cassytha filiformis showed an increase in succinic dehydrogenase activity in peripheral zones of the penetrating haustorium into the host tissues. Activity in the mature haustorium was localized in the areas of endophyte in Cuscuta reflexa.

In the present genomic era, the discovery of new generation of non-Sanger-based sequencing technologies plays an immense role in developing precise, rapid and economical genome sequencing instruments. Advent of Next Generation Sequencing (NGS) technology has impressive scientific and novel biological applications targeting the elevated human health...

Multiple regression equations were derived from the quantitative data of fresh leaf parameters such as endogenous of level polyphenol oxidase (PPO) and peroxidise (PO), substrates (polyphenols and catechins) and enzymes (sum of PPO and PO) to substrate (polyphenols) ratio. Highly correlated green leaf parameters and their corresponding made tea con...

Direct organogenesis and in vitro flowering was obtained in Vitex trifolia. L an endangered medicinal plant. High frequency and maximum number of multiple shoots were obtained from shoot tip explants on MS medium supplemented with BAP (3.96-15.85 μM) and IAA (5.70-22.83 μM). Regenerates, when transferred to rooting medium with IBA at concentration...

Plectranthus barbatus (syn. Coleus forskohlii) is the only known source of forskolin, a compound with a wide range of pharmacological activities. Here, an efficient protocol for adventitious root regeneration from leaf explants of P. barbatus was developed. Different concentrations of plant growth regulators individually and in combination were use...

The present contribution focused on standardization of in-vitro propagation protocols of A.lanata, an important medicinal plant used in diverse traditional system of medicine. Explants cultured under in-vitro conditions on nutrient media supplemented with auxins and cytokinins showed variation in growth response to different season’s during plant r...

Morphological diversity of three Camellia (Theaceae) taxa conserved in an ex situ gene bank was studied and the importance of different descriptors in categorising accessions into distinct groups was also determined. Twelve accessions were characterised using 15 morphological descriptors of IPGRI guidelines. The results of principal component analy...

Rhizobium species have the ability to infect roots of leguminous plants, form nodules and work symbiotically with their host in fixing molecular nitrogen. In this paper, morphological and biochemical characteristization of Rhizobium species isolated from Trigonella foenumgraecum and Mimosa pudica were reported. Bergey's manual was followed for morp...

Alpha-amylase is one of the most widely used enzymes commercially, produced at a large scale in many industries like food processing, feed, detergents, therapeutics and breweries. The conventional method of producing alpha-amylases in most of the industries is by solid-state fermentation using different species of fungi and bacteria. In the present...

Polyphenoloxidase and peroxidase enzymes were extracted and the isozyme banding pattern was studied in twelve south Indian tea clones of Camellia species conserved an ex situ gene bank. Banding pattern exhibited variations at inter specific and clonal level. A maximum of 6 isozymes were observed for polyphenol oxidase in UPASI 2 clone and a minimum...

Anatomical studies of leaf and stem of twelve clones of Camellia were investigated. Cross sections of the stem of all the clones exhibited a typical pattern of arrangement of tissues characteristics of woody plants. Two types of idioblastic sclereids were found in the medullary parenchyma of the taxa studied. While astrosclereids werepresent in 10...

The present study investigates the qualitative and quantitative analysis of the major bioactive constituents of different parts like root, stem and leaf of medicinally important plant Dryptes roxburghii using seven different solvents. Qualitative study of alkaloids, carbohydrates, glycosides, steroids, flavonoids, coumarins, saponins, fatty acids,...

The application of plant nutrients through recycling of sericulture farm residues as compost, vermicompost and wise use of chemical fertilizers in mulberry cultivation can facilitate quality leaf production for better and sustainable silkworm cocoon production. Composting of serifarm residues can be carried out by employing any of three proven meth...

A validation trial has been conducted for the package developed for exclusive chawki mulberry garden for its popularization. The study was carried out under institute village linkage programme (IVLP) of the CSR&TI, Mysore by establishing a new chawki mulberry garden in 2 acres with four blocks module of 0.5 acre each, with V1 and S36 mulberry varie...

In the present investigation, the occurrence of phloem and callose deposition on sieve plates in the haustorium of Aeginetia pedunculata Wall., a root holoparasitic herb of Orobanchaceae, is demonstrated. Our studies reveal that the haustorium of A. pedunculata has highly specialized phloem tissue, comprising of sieve tube elements and companion ce...

The mulberry plant and specifically its leaves are the first stage in the biological process of silk production. Mulberry cultivation at low cost is crucial to the survival and growth of sericulture and the silk industry. The authors here outline an environmentally friendly approach that is also focused on low cost production. Table 1 is useful in...