Ksymena Chirkowska

TU Dublin | TU Dublin · School of Chemical and Pharmaceutical Sciences

Can someone explain how to approach this question step by step?

What are the concentration of the following solutions of drugs in g/100ml and mg/100ml

Carbimazole A(1%, 1 cm) value =557 at 291nm, measured absorbance 0.557 at 291 nm.

Hydrocortisone sodium phosphate, A(1%, 1 cm) value 333 at 248 nm, measured absorbance 0.666 at 248 nm.

I was carrying out this experiment in college and I am unsure if my plasmid is present in the sample I prepared, as there is visible "smudging"on my sample. I was wondering if anyone could help me idenitfy if its there and why my sample looks like this.

Markers are at lane 1 and 19, with my sample at lane 14.

I was isolating puc19 plasmid in the lab today and I was wondering why we had to let the sample dry from the ethanol used before using it on gel electrophoresis