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DOI: 10.14260/jemds/2014/3264
ORIGINAL ARTICLE
J of Evolution of Med and Dent Sci/ eISSN- 2278-4802, pISSN- 2278-4748/ Vol. 3/ Issue 38/Aug 25, 2014 Page 9768
DOES IMMUNOTHERAPY IN ALLERGIC RHINITIS WITH INCREASED IGE
LEVELS OFFER CONSISTENT RELIEF FROM NASAL SYMPTOMS, AN
INSTITUTIONAL STUDY?
V. Krishna Chaitanya1, T. B. Ramakrishna2, N. Janardhan3, S. Rajesh Kumar4
HOW TO CITE THIS ARTICLE:
V. Krishna Chaitanya, T. B. Ramakrishna, N. Janardhan, S. Rajesh Kumar. “Does Immunotherapy in Allergic
Rhinitis with Increased IGE Levels offer Consistent Relief from Nasal Symptoms, an Institutional Study?” Journal
of Evolution of Medical and Dental Sciences 2014; Vol. 3, Issue 38, August 25; Page: 9768-9776,
DOI: 10.14260/jemds/2014/3264
ABSTRACT: OBJECTIVES: 1. To evaluate the efficacy of Immunotherapy in improvement of nasal
symptoms in patients with Allergic Rhinitis. 2. To evaluate the adverse reactions of Immunotherapy
in patients suffering with allergic rhinitis. MATERIALS AND METHODS: Study group included 138
patients with Allergic rhinitis, who presented in Department of ENT, during July 2011 to March 2014.
Patients with clinical history of allergic rhinitis were screened with total serum IgE. Patients with
elevated total serum IgE were investigated with skin prick test. Patients with positive skin reactions
were treated with sub cutaneous immunotherapy. Development of adverse reactions during
administration of immunotherapy was assessed. A follow up period of 18 months was considered for
the study. A relief in clinical symptoms of allergic rhinitis at the end of 18 months was considered.
CONCLUSION: Immunotherapy offers significant improvement in relief of clinical symptoms of
allergic rhinitis with minimal adverse reactions over a long time. Total serum IgE forms an affective
screening tool in diagnosis of allergic rhinitis. Immunotherapy appears as a safe alternative treatment
for the management of allergic rhinitis.
KEYWORDS: Allergic Rhinitis, Immunotherapy, Allergen sensitivity, Total serum IgE, Skin prick test,
adverse reactions.
INTRODUCTION: Allergic rhinitis is the most common allergic disease affecting the human
population. Apart from causing nasal congestion, difficulty in breathing, sneezes, Allergic rhinitis also
presents with fatigue, difficulty in concentrating, poor school and work performance. Most common
investigations preferred in diagnosing allergic rhinitis are elevated total serum IgE and skin prick
test. (1)
Patients of allergic rhinitis with a positive clinical history on exposure to the allergen often
show specific IgE antibodies against the offending allergens.(2) RAST or ELISA are preferred to be
more precise investigations in diagnosis but purified proteins and antibodies are not available for
characterization based on major allergen content in India.(3)
The main stay of management of allergic rhinitis is focused on suppression of inflammatory
reactions by medications like antihistamines, nasal steroids, and leukotriene receptor antagonists.
Apart from these since long time Immunotherapy is recognized as an alternative therapeutic
option to alter the natural history of allergic rhinitis. It alters the biochemical nature of the disease by
a shift of TH2 response towards TH1 and down regulates IL-4. This will lead to a decrease in release
of inflammatory mediators, specific IgE levels, allergen specific airway hyper-responsiveness
producing clinical improvement of symptoms.(4)
DOI: 10.14260/jemds/2014/3264
ORIGINAL ARTICLE
J of Evolution of Med and Dent Sci/ eISSN- 2278-4802, pISSN- 2278-4748/ Vol. 3/ Issue 38/Aug 25, 2014 Page 9769
OBJECTIVES:
1. To evaluate the efficacy of Immunotherapy in improvement of nasal symptoms in patients with
Allergic Rhinitis.
2. To evaluate the adverse reactions of Immunotherapy in patients suffering with allergic rhinitis.
METHODOLOGY:
Study Population: Study group included 138 patients of Allergic Rhinitis with symptoms persisting
for more than 3 months, who presented in Department of Otolaryngology, Narayana Medical College
and Hospital, Chinthareddypalem, Nellore during July 2011 to March 2014.
Inclusion Criteria: Patients with a clinical history of allergic rhinitis with elevated total Serum IgE
were included in the study in addition to symptoms of nasal itching, sneezing and rhinorrhea for
more than 3 months with itching disturbing sleep in night, sneezing attacks of 5 or more per episode
with minimum 5 episodes per day and rhinorrhea if patient has to use hand kerchief to wipe off
mucous secretions at least once during a day were included.
Exclusion Criteria: Patients of Allergic rhinitis less than 3 months with normal values of total serum
IgE, Patients of Bronchial asthma, Atopic dermatitis, patients with local nasal diseases like deviated
nasal septum, Turbinate Hypertrophy, Rhino sinusitis, Nasal polyposis were strictly excluded from
the study.
INTERVENTIONS:
Initial selection of patients for the study was based on a clinical evaluation by a panel of three
Doctors experienced in management of Allergic rhinitis. After obtaining a written informed consent
from the patients for the treatment and follow up they were evaluated with a clinical questionnaire
for nasal itching, sneezes and rhinorrhea. These patients with positive clinical history of allergic
rhinitis were investigated with total serum IgE. Total serum IgE exceeding 500 IU/ml was considered
elevated in the study group. (5)
Skin prick test was performed in patients having elevated total serum IgE. A total number of
eleven allergens which included Pollen, dusts, Mite and fungi were selected for the study. Patients
with positive skin reactions were given sub cutaneous immunotherapy. We selected a total of 11
allergens Dusts - common House dust, wood and spider web dust, mite- Dermatophagoides, Fungus -
Aspergillus and Pollen from Xanthium, Parthenium, Argemone and Brassica considering the local
climatic conditions.(6) Other specific allergens which include epithelia, insects and food allergens
were excluded.
Skin prick test is performed on the fore arm with positive and negative controls. Histamine
diphosphate is used as positive control to compare the cutaneous response to injected allergen
extracts. Diluent normal saline buffer is used as negative control to rule out the possibility of getting
false positive skin response due to trauma or sensitization induced by skin test device.(7)
Drugs which alter the response of the test like Antihistamines are discontinued for 3 weeks
prior to skin test. The allergen drops were deposited in longitudinal rows, 3 to 3.5 cm apart. In the
prick technique, a drop of an allergen is applied on the skin. The skin is then pricked using a sterile
blood lancet.
DOI: 10.14260/jemds/2014/3264
ORIGINAL ARTICLE
J of Evolution of Med and Dent Sci/ eISSN- 2278-4802, pISSN- 2278-4748/ Vol. 3/ Issue 38/Aug 25, 2014 Page 9770
Each blood lancet is used only once so as to avoid contamination. Grading of skin reaction is
done after 20 minutes in comparison with negative control normal saline and positive control
Histamine diphosphate. Skin prick test reactions with more than 3mm wheal were considered as
positive.
Skin prick test wheal
Result
Grading
0mm
Negative
Up to3mm
Positive
1+
3-5mm
Positive
2+
5-7mm
Positive
3+
7mm and above
Positive
4+
Table 1: Grading of skin prick test response(8)
Results of skin prick test were observed based on the response obtained in the form of wheal
to each allergen. Depending on the response to allergen the results were graded and a mixture of
solution was prepared in optimal concentration to each allergen after considering the cross reactions.
Immunotherapy is administered subcutaneously in all the patients.
Immunotherapy was started with 1:5000 w/v diluted antigen and the injections are given
two times a week starting from 0.05 ml and increased in every injection by 0.05 ml, increased to
0.1ml. In the patients who were not able to tolerate an incremental dosage of 0.1ml per injection
dosage is titrated and continued at 0.05ml per injection.
The injections are given subcutaneously with insulin syringe with increased concentrations as
per the schedule. The further schedule is as follows:1:5000 – once in three days, from 0.05 ml to 0.9
ml with an increase in dose by 0.1ml per each injection, 1:500 – once in three days, from 0.1 ml to 0.9
ml in increments of 0.1ml, 1:50 – one time a week, from 0.05 ml to 0.5 ml, 1:50 – one time in 2 weeks
at a dose of 0.6 ml, 1:50 – one time in 3 weeks at a dose of 0.7 ml, 1:50 – one time a month at a dose of
0.8 ml, 1:50 – one time a month at 0.9 ml, 1:50 – one time a month at 1.0 ml which we considered
Highest maintenance dose.(8) After each injection patients were observed for acute hypersensitivity
reactions.
Main Outcomes Measures: Patients were instructed to note the number of episodes of nasal itching,
sneezes and rhinorrhea during immunotherapy and follow up. We preferred a Clinical improvement
of symptoms of allergic rhinitis if there was a decrease in appearance of symptom severity. A
decrease in number of episodes of nasal itching to less than 2 in a month, sneezes to less than 3
episodes per month with not more than 2 sneezes per episode and an improvement of rhinorrhea if
the use of hand kerchief to wipe off nasal secretions limited to not more than 2 episodesin a month, in
a patient as positive responders to immunotherapy. In all these patients total serum IgE was repeated
at the end of follow up of 18 months.
We observed and enquired the patients for the development of adverse reactions to
Immunotherapy like tightness of chest, difficulty in breathing, abdominal pain, development of
intense skin itching, swelling of lips or tongue immediately after the injection and lassitude,
headache, papular rash over the malar bones, gastro intestinal disturbances, photo dermatitis over
the exposed areas and a change of voice when the patient came for the next injection for late phase
DOI: 10.14260/jemds/2014/3264
ORIGINAL ARTICLE
J of Evolution of Med and Dent Sci/ eISSN- 2278-4802, pISSN- 2278-4748/ Vol. 3/ Issue 38/Aug 25, 2014 Page 9771
allergic reactions during the course of immunotherapy.(9) Also we enquired the patient for the
development of similar adverse reactions during the follow up period after immunotherapy.
RESULTS: A total No. of 138 patients with Allergic Rhinitis formed the study population. Of them 109
(78.99%) patients showed elevated levels of total Serum IgE. These 109 patients with elevated total
serum IgE were investigated with skin prick test. Prior to Immunotherapy Skin prick test revealed
allergen sensitization in 92(84.40%) for pollen, 97(88.99%) for dusts and 87 (79.81%) for mite and
fungi. Most of the patients had a mixed response to allergens in skin prick test. These results were
documented in table 2.
Prior to Immunotherapy it was observed that in allergen sensitive patients to pollen nasal
itching was seen in 89 (81.65%), to dusts in 92 (84.40%) and to mite and fungi in 79 (72.48%).
Allergen sensitivity to Sneezes revealed that 84 (77.06%) patients sensitive to pollen, 89 (81.65%) to
dusts and 82 (75.23%) to mite and fungi. Episodes of Rhinorrhea were observed in 87(79.81%)
patients on exposure to Pollen, 91 (83.49%) to dusts and 83(76.14%) to mite and fungi. These results
were documented in tables 3, 4, 5.
A decrease in number of episodes of clinical symptoms of allergic rhinitis was observed for
nasal itching, sneezes and rhinorrhea during follow up. Episodes of nasal itching were reduced by
86.78%, Sneezes were reduced by 89.53%andepisodes of rhinorrhea were reduced to 87.63%at the
end of 18 months. These results were evident in the form of a graph and represented in figure 1.
Post immunotherapy allergen sensitivity was reduced for Nasal itching in 72(80.90%)
patients for pollen, 71 (77.17%) to dusts and 61 (77.21%) to mite and fungi. Sneezes reduced in
65(77.38%) patients to pollen, 66 (74.15%) to dusts and 58 (70.73%) to mite and fungi.
Similarly episodes of rhinorrhea were reduced in 66 (75.86%) patients to pollen, 69
(75.82%) to dusts and 64 (77.10%) to mite and fungi. These results were documented in tables 3, 4,
5. Post Immunotherapy total serum IgE estimation repeated at the end of follow up. Elevated total
serum IgE was seen in 23 (21.10%) of patients.
During Immunotherapy we observed itching and redness in13 (11.92%) patients at the site of
injection which subsided without any medication. 7 (6.42%) patients expressed lassitude during the
course of Immunotherapy. 2 (1.83%) patients developed abdominal pain. One patient developed
intense itching all over the body during immunotherapy.
In our study we observed that there was no change in blood pressure, difficulty in breathing
or chest tightness and none of our patients developed lip or tongue swelling or other severe systemic
anaphylactic reactions in early or late phases of Immunotherapy. Also no adverse reactions were
noted during follow up.
Skin prick test
positive
Negative
Total
Pollen
92
17
109
Dusts
97
12
109
Mite and Fungi
87
22
109
Table 2: Allergen sensitivity of Skin prick test
DOI: 10.14260/jemds/2014/3264
ORIGINAL ARTICLE
J of Evolution of Med and Dent Sci/ eISSN- 2278-4802, pISSN- 2278-4748/ Vol. 3/ Issue 38/Aug 25, 2014 Page 9772
Nasal Itching
Pre treatment
Post treatment
Relief
Pollen
89
17
72(80.90%)
Dusts
92
21
71(77.17%)
Mite and Fungi
79
18
61(77.21%)
Table 3: Specific allergen response to Nasal Itching in Immunotherapy
Sneezes
Pre treatment
Post treatment
Relief (%)
Pollen
84
19
65(77.38%)
Dusts
89
23
66(74.15%)
Mite and Fungi
82
24
58(70.73%)
Table 4: Specific allergen response to Sneezes in Immunotherapy
Rhinorrhea
Pre treatment
Post treatment
Relief (%)
Pollen
87
21
66(75.86%)
Dust
91
22
69(75.82%)
Mite and Fungi
83
19
64(77.10%)
Table 5: Specific allergen response to Rhinorrhea in Immunotherapy
Figure 1: Chart showing assessment of clinical Symptoms of nasal itching, sneezes, rhinorrhea during
follow up of Immunotherapy.
DISCUSSION: An assessment of the response of immunotherapy in allergic rhinitis is done by
assessing an improvement of clinical symptoms, serological investigations like total serum IgE, skin
tests, and estimation of specific immunological parameters. (10)
Fig. 1
DOI: 10.14260/jemds/2014/3264
ORIGINAL ARTICLE
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After completion of immunotherapy, we observed relief in clinical symptoms of Allergic
rhinitis which were maintained during the follow up.
In our study post immunotherapy at the end of 18 months of follow up we observed an
individual relief in symptoms of allergic rhinitis in patients desensitized for pollen where
80.90%relief was observed for nasal itching, 77.38%patients were relieved from sneezes and
episodes of rhinorrhea reduced in75.86%
Following immunotherapy to dusts it was observed that the episodes of Clinical symptoms of
allergic rhinitis were reduced in77.17% patients for nasal itching, sneezes in74.15% and episodes of
rhinorrhea in75.82% in patients at the end of 18 months. Similarly in patients desensitized to mite
and fungi a decrease in episodes of nasal itching was seen in 77.21% of patients, sneezes were
reduced in 70.73% of patients and episodes of rhinorrhea in77.10% of patients.
In our study a relief of symptoms of nasal itching for all the allergens post immunotherapy
was observed in 78.42% of patients. Similarly in a study by Yuri Zeldin et al (11) it was observed that a
decrease in episodes of nasal itching was seen in 62% of patients. In another study by Rao Sukhesh
(12) there was a relief in nasal itching in 65.3% of patients.
In our study relief from sneezes to all allergens was seen in 74.08% of patients post
immunotherapy. Yuri Zeldin et al (11) observed a reduction in sneezes in 72.5% of population. Rao
Sukhesh (12) observed a reduction in sneezes in61.5% of patients. Episodes of rhinorrhea decreased
collectively to all allergens in 76.26% of patients in our study.
Yuri Zeldin et al (11) observed a decrease in episodes of rhinorrhea in 84.5% of patients. Rao
Sukhesh (12) observed a decrease in symptoms of rhinorrhea in 80.77% of patients. Yuri Zeldin et al(11)
considered improvement in quality of life with visual analogue scale in their study which can be a
factor for the percentage variation. Similarly In the study by Rao Sukhesh (12) we observed symptoms
were graded as mild, moderate and severe. This can lead to a variation in response of symptom relief
from our study.
In the present day scenario immunotherapy is indicated as a supplement to allergen
avoidance and to pharmacotherapy.(13) In a study conducted by Shyam et al(14) in patients of Allergic
rhinitis, it was observed that post immunotherapy and follow up there was a reduction in symptoms
of allergic rhinitis of sneezes, Rhinorrhea, nasal block, redness of eyes, and headache in76% of
patients. In this study symptom relief was studied at the end of 18 months.
In the study by Yuri Zeldin et al(11) it was observed that 73% of patients had a relief of overall
symptoms of allergic rhinitis collectively where the symptom relief was analyzed at the end of 18
months. In the study by Rao Sukhesh(12) 69.21% of patients had relief in overall symptoms
collectively.
In our study a collective decrease in symptoms of allergic rhinitis was seen to all the allergens
was seen in 76.25% of patients at the end of 18 months. We infer that following immunotherapy a
relief in symptoms of allergic rhinitis ranging from 69% to 76% can be obtained with a symptom free
life over a long period.
Initially we investigated all the patients with clinical symptoms of allergic rhinitis patients
with total serum IgE. Total serum IgE is believed to be elevated in cases of allergic rhinitis. In a study
by V.S Chowdary et al (15) they opined that estimation of levels of total serum IgE in patients of allergic
rhinitis helps in proper planning of treatment of the disease. Total serum IgE was repeated at the end
of 18 months in our study.
DOI: 10.14260/jemds/2014/3264
ORIGINAL ARTICLE
J of Evolution of Med and Dent Sci/ eISSN- 2278-4802, pISSN- 2278-4748/ Vol. 3/ Issue 38/Aug 25, 2014 Page 9774
We observed that the levels of total serum IgE returned to normal in 86 (78.89%) of patients.
According to Durham et al (16) who opined that after the successful completion of immunotherapy and
at follow up post Immunotherapy there will be a reduction in release of inflammatory mediators and
specific IgE levels. In a study by S. Manohar et al(17) it was reported that the levels of total serum IgE
returned to normal in 90.2% of patients of allergic rhinitis and asthma.
We observed adverse reactions in the form of itching and redness in 11.92%, lassitude in
6.42% and 1.83% of patients developed abdominal pain and 0.91% of patients developed intense
itching all over the body during immunotherapy.
In a study by J. Siedenberg et al(18) adverse reactions in the form of pruritus were seen in
79(40.93%), gastro intestinal disorders were seen in 4(2.07%), tiredness or lassitude seen in
1(0.51%), erythema in 9(4.66%). These findings suggest that Immunotherapy can be an effective
alternative management with minimal adverse reactions apart from the other modalities of
treatment available.
In our study we observed that estimation of total serum IgE forms an effective screening
measure in diagnosis of Allergic rhinitis along with clinical history. Further investigation of these
patients with skin prick test confirms the nature of the disease.
When these patients were given immunotherapy an improvement in clinical symptoms of
allergic rhinitis was observed. Successful administration of immunotherapy depends on the proper
patient selection, maintenance of quality of allergens, adequate titration of the doses of allergens,
proper monitoring the timing and dosage of the allergens and follow up. Proper care has to be taken
in the treatment with a careful observation to the development of adverse reactions which were to be
monitored.
CONCLUSIONS: Immunotherapy offers significant improvement in relief of clinical symptoms of
allergic rhinitis with minimal adverse reactions over a long time. Total serum IgE forms an affective
screening tool in diagnosis of allergic rhinitis. Immunotherapy appears as a safe alternative treatment
for the management of allergic rhinitis.
ACKNOWLEDGMENTS: We express our sincere thanks to Chair person, Dr.P.Narayana,Medical
Director Dr. Subramanyam, Principal Dr. Veeranagireddy, Medical Superintendent Dr. Veera Prasad
and to the management and staff Narayana Medical College for making this study a success.
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DOI: 10.14260/jemds/2014/3264
ORIGINAL ARTICLE
J of Evolution of Med and Dent Sci/ eISSN- 2278-4802, pISSN- 2278-4748/ Vol. 3/ Issue 38/Aug 25, 2014 Page 9776
AUTHORS:
1. V. Krishna Chaitanya
2. T. B. Ramakrishna
3. N. Janardhan
4. S. Rajesh Kumar
PARTICULARS OF CONTRIBUTORS:
1. Assistant Professor, Department of ENT,
Narayana Medical College and Hospital,
Nellore.
2. Professor and HOD, Department of ENT,
Narayana Medical College and Hospital,
Nellore.
3. Professor, Department of ENT, Narayana
Medical College and Hospital, Nellore.
4. Associate Professor, Department of ENT,
Narayana Medical College and Hospital,
Nellore.
NAME ADDRESS EMAIL ID OF THE
CORRESPONDING AUTHOR:
Dr. V. Krishna Chaitanya,
Assistant Professor,
Department of ENT,
Quarters No. 98,
Narayana Medical College and Hospital,
Chinthareddypalem,
Nellore-524001, Andhra Pradesh.
Email: drvkc17@gmail.com
Date of Submission: 15/08/2014.
Date of Peer Review: 16/08/2014.
Date of Acceptance: 20/08/2014.
Date of Publishing: 23/08/2014.
