Klaus MummenhoffUniversität Osnabrück | UOS · FB 5 Biologie/Chemie
Klaus Mummenhoff
apl. Prof.
About
264
Publications
54,506
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
6,877
Citations
Publications
Publications (264)
The genus Thlaspi L. s.l. comprises numerous segregate lineages, which have been recognized as single genera. One of these, Microthlaspi, represents one such segregate. It consists of morphologically similar annual species. The species have different ploidy levels (2x, 4x, 6x) and are usually summarized cinder the designation Thlaspi perfoliatum ag...
![Figure 2. Brassicales phylogeny. Brassicales phylogeny [105] with...](profile/Klaus-Mummenhoff/publication/49701364/figure/fig1/AS:664965367201794@1535551506517/Brassicales-phylogeny-Brassicales-phylogeny-105-with-numbers-of-genera-and-species_Q320.jpg)
Life without the mustard family (Brassicaceae) would be a world without many crop species and the model organism Arabidopsis (Arabidopsis thaliana) that has revolutionized our knowledge in almost every field of modern plant biology. Despite this importance, research breakthroughs in understanding family-wide evolutionary patterns and processes with...
The best documented survival responses of organisms to past climate change on short (glacial-interglacial) timescales are distributional shifts. Despite ample evidence on such timescales for local adaptations of populations at specific sites, the long-term impacts of such changes on evolutionary significant units in response to past climatic change...
This is a detailed comment on a "Research Highlight Contribution":
On the age of the mustard family (Brassicaceae)
Andreas Franzke^a^, Marcus A. Koch^b^, Thomas L.P. Couvreur^c^, Martin A. Lysak^d^, and Klaus Mummenhoff^e^
^a^Botanical Garden, Heidelberg Institute of Plant Sciences, Heidelberg University, D-69120 Heidelberg
^b^Department Biodiver...
a b s t r a c t Systematics of the genus Isatis (Brassicaceae) is difficult and controversial, and previous studies were based solely on morphological characters. Sequence variation of the internal transcribed spacer (ITS) regions and the 5.8S gene of nuclear ribosomal DNA (nrDNA) were analyzed using parsimony and Bayesian methods. Twenty-eight tax...
Unlabelled:
Premise of the study:
As a crop and medicinal plant, the octoploid Andean endemic Lepidium meyenii suffers from taxonomic uncertainty. Few molecular markers are available to genotype individuals or track gene flow in wild and cultivated material. •
Methods and results:
Using available sequence data, eight cpSSR primer pairs were de...
Mesopolyploid whole-genome duplication (WGD) was revealed in the ancestry of Australian Brassicaceae species with diploid-like chromosome numbers (n = 4 to 6). Multicolor comparative chromosome painting was used to reconstruct complete cytogenetic maps of the cryptic ancient polyploids. Cytogenetic analysis showed that the karyotype of the Australi...
Most species of the Brassicaceae develop fruits in which seeds are released through a process termed fruit dehiscence. To provide a phylogenetic perspective for fruit dehiscence, the Arabidopsis model system will be widened here by two other systems from Brassicaceae. Some genera in Brassicaceae develop indehiscent fruits that do not release ripe s...
Sequence data from the nuclear encoded ribosomal internal transcribed spacer (ITS) region were used to determine monophyly
of tribes, tribal limits, and tribal relationships of 96 so far unassigned or tentatively assigned genera (represented by
101 taxa/accessions) within the Brassicaceae. Maximum-parsimony and maximum-likelihood analyses of 185 IT...
The small Southwest Asian scree plant genus Heldreichia Boiss. (Brassicaceae) with its diversity centre in Anatolia, is revised in a morphological and molecular (ITS sequence) monograph. Heldreichia taxa under study appear to be monophyletic in the Bayesian tree; the tribal position remains unclear. A re-evaluation of morphological characters resul...
The small Southwest Asian scree plant genus Heldreichia Boiss. (Brassicaceae) with its diversity centre in Anatolia, is revised in a morphological and molecular (ITS sequence) monograph. Heldreichia taxa under study appear to be monophyletic in the Bayesian tree; the tribal position remains unclear. A re‐evaluation of morphological characters resul...
Due in part to its distinctive and variable morphology, traditional taxonomy has not resolved the systematic position of Aethionema trinervium Boiss., which has been previously placed in several different genera including Aethionema R. Br., Eunomia DC., Hutchinsia R. Br., and Thlaspi L. Using sequence data from the ITS-1 and ITS-2 regions of riboso...
Brassicaceae is an important family at both the agronomic and scientific level. The family not only includes several model species, but it is also becoming an evolutionary model at the family level. However, resolving the phylogenetic relationships within the family has been problematic, and a large-scale molecular phylogeny in terms of generic sam...
Incongruence between chloroplast and nuclear DNA phylogenies, and single additive nucleotide positions in internal transcribed spacer (ITS) sequences of polyploid Australian/New Zealand (NZ) Lepidium species have been used to suggest a bicontinental hybrid origin. This pattern was explained by two trans-oceanic dispersals of Lepidium species from C...
Watering of Phalaenopsis and Arabidopsis plants with an aqueous colloidal solution of NaYF4:Yb,Er nanoparticles leads to uptake and transport of nanoparticles into the plants within a few days. Characteristic upconversion emission of the particles can be excited in the shoot and the leaves of the plants. Uptake of particles by the root was studied...
The Brassicaceae family is of great scientific interest because it contains the plant model organism Arabidopsis thaliana. Currently, contemporary plant research activities expand to other Brassicaceae taxa. Despite that, the phylogeny of this family is only partly understood. The present study deepens our understanding of a family-wide phylogeny b...
Phylogenetic analysis of 155 nuclear rDNA ITS sequences among them 19 Iranian endemic genera were used to elucidate phylogenetic relationships of Old World Brassicaceae from Iran in the context of the most recent tribal system suggested by Al-Shehbaz et al. [Al-Shehbaz, I.A., Beilstein, M.A, Kellogg, E.A., 2006. Systematics and phylogeny of the Bra...
Fruits represent a key innovation of the flowering plants that facilitates seed dispersal. In many species of the plant family
Brassicaceae dehiscent fruits develop in which seed dispersal occurs through a process termed ‘pod-shatter’. In the case of
dehiscence, the fruit opens during fruit maturation. Phylogeny reconstructions using molecular mark...
Acanthocardamum is a monotypic genus in the Brassicaceae restricted to the alpine region of the Zagros mountains in Iran. Earlier authors have suggested the genus to be close to either Lepidium or Aethionema. To elucidate the systematic position of the genus, ITS sequence data of Acanthocardamum erinaceum were analyzed together with representatives...
In previous taxonomic literature, the monotypic genus BrossardiaBoiss. was suggested to be related to Thlaspi L. s.l. and AethionemaR.Br. ITS sequence data of Brossardia papyracea * were added to a previously published data set, which includes sequences of representative taxa of Aethionema and Thlaspi s.l., to elucidate the systematic position of B...
The polypeptide composition of the large and small subunits of ribulose-1,5-bisphosphate carboxylase/oxy-genase (RUBISCO) from Cardaria draba (L.) DESV. (Brassicaceae) and from representatives of the related genus Lepidium L. have been studied by isoelectric focusing (IEF) analysis. In the literature Cardaria DESV. is either treated as a separate g...
Like island-endemic taxa, whose origins are expected to postdate the appearance of the islands on which they occur, biome-endemic taxa should be younger than the biomes to which they are endemic. Accordingly, the ages of biome-endemic lineages may offer insights into biome history. In this study, we used the ages of multiple lineages to explore the...
Allopolyploidy and long-distance dispersal, respectively, are regarded as prominent factors in plant evolution and biogeography. Here we review the rare cases of prehistorical (not man-mediated) intercontinental long-distance dispersal of plants combined with allopolyploidy. All examples given indicate a late Ter-tiary/Quaternary evolution of the p...
In order to examine the systematic application of seed-coat microsculpturing in Isatis, seed surfaces of 23 species (41 populations) in four genera of tribe Isatideae were examined using scanning electron microscopy (SEM). Eight types of basic ornamentation patterns were recognized among the studied specimens. Of these, the reticulate–areolate type...
In wide-ranging species, the genetic consequences of range shifts in response to climate change during the Pleistocene can be predicted to differ among different parts of the distribution area. We used amplified fragment length polymorphism data to compare the genetic structure of Arabis alpina, a widespread arctic-alpine and afro-alpine plant, in...
Abstract
In wide‐ranging species, the genetic consequences of range shifts in response to climate change during the Pleistocene can be predicted to differ among different parts of the distribution area. We used amplified fragment length polymorphism data to compare the genetic structure of Arabis alpina, a widespread arctic‐alpine and afro‐alpine p...
The Brassicaceae is a large plant family (338 genera and 3,700 species) of major scientific and economic importance. The taxonomy of this group has been plagued by convergent evolution in nearly every morphological feature used to define tribes and genera. Phylogenetic analysis of 746 nrDNA internal transcribed spacer (ITS) sequences, representing...
Arabis alpina is a characteristic plant in arctic-alpine habitats and serves as a classical example to demonstrate biology, ecology and biogeography of arctic-alpine disjuncts. It has a wider distribution than most other arctic-alpine plants, covering all European mountain systems, the Canary Islands, North Africa, the high mountains of East Africa...
The genera Brachycarpaea, Cycloptychis, Schlechteria, Silicularia, and Thlaspeocarpa are united with Heliophila (Brassicaceae). Except for Brachycarpaea, the genera are treated as new synonyms of Heliophila. The characters previously used to distinguish these genera are critically evaluated. The retention of Brachycarpaea and Silicularia in Helioph...
Heliophila (ca. 73 spp.), the ditypic Cycloptychis and Thlaspeocarpa, and the monotypic Schlechteria, Silicularia, Brachycarpaea, and Chamira are endemic to the Cape region of South Africa, where they are the dominant genera of Brassicaceae. They may be regarded as the most diversified Brassicaceae lineage in every aspect of habit, leaf, flower, an...
Several vegetation belts stretch continuously from Europe to Asia, taiga and steppe being most prominent. Numerous plant species within these belts share a conspicuous distribution area, which is longitudinally contracted or disrupted approximately along longitude 70 degrees E. To date no hypothesis for this intriguing distribution pattern has been...
Lepidium sensu stricto (s.s.) (Brassicaceae) (ca. 150 species) is distributed worldwide with endemic species on every continent. It is represented in Australia and New Zealand by 19 and seven native species, respectively. In the present study we used a nuclear ribosomal internal transcribed spacer (ITS) phylogeny in comparison with a cpDNA phylogen...
Using sequence data from the ITS region (internal transcribed spacers ITS1 and ITS2 of nuclear ribosomal DNA and the 5.8S rRNA gene) and chloroplast DNA sequence data from the trnL intron. we examined the evolutionary relationships of Old and New World Smelowskia species with its putatively related genera (Brassicaceae). ITS and trnL sequence data...
The present review summarizes results from the past decade on the systematics, population genetics, and evolutionary biology of the mustard family, Brassicaceae (Cruciferae). The research of various authors is discussed and presented in the context of ongoing and accumulating studies. The review is useful in view of the immensely increasing work on...
Understanding the pattern of speciation in a group of plants is critical for understanding its morphological evolution. Lepidium is the genus with the largest variation in floral structure in Brassicaceae, a family in which the floral ground plan is remarkably stable. However, flowers in more than half of Lepidium species have reduced stamen number...
The present review summarizes results from the past decade on the systematics, population genetics, and evolutionary biology of the mustard family, Brassicaceae (Cruciferae). The research of various authors is discussed and presented in the context of ongoing and accumulating studies. The review is useful in view of the immensely increasing work on...
Questions
Questions (7)
We developed a rapid viability test for plant seeds in which yeasts metabolize organic compounds that leach from seeds (dependent on the physiological age of the seed) and thereby reduce our redox-indicator resazurin, accompanied by a color change (5). Unfortunately, seeds of some species acidify our test solution causing a pH-dependent colour change of the resazurin (abiotic reaction) which distorts our test results (6). We are looking to replace our redox indicator dye for these cases!
We already researched on several dyes from the tetrazolium-family that unfortunately also change their colour upon acidification, i.e., MTT (1), TTC (2), MTS (3) or the solubility is severely limited, e.g. MTT (3), XTT (4).
We are grateful for any advice!
1: Plumb et al. (1989) Effects of the pH Dependence of 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium Bromide-Formazan Absorption on Chemosensitivity Determined by a Novel Tetrazolium-based Assay. Cancer Res 49: 4435–4440.
2: Lopez Del Egido et al. (2017) A Spectrophotometric Assay for Robust Viability Test of Seed Batches Using 2,3,5-Triphenyl Tetrazolium Chloride: Using Hordeum vulgare L. as a Model. Front. Plant Sci. 8: 747. doi: 10.3389/fpls.2017.00747
3: Riss et al. (2013) Cell Viability Assays. https://www.ncbi.nlm.nih.gov/books/NBK144065/
4: Goodwin et al. (1995) Microculture tetrazolium assays: a comparison between two new tetrazolium salts, XTT and MTS. J Immunol Methods 13: 95-103. doi: 10.1016/0022-1759(94)00277-4
5: Mohammed et al. (2019) Dead or Alive: Simple, Non-destructive, and Predictive Monitoring of Seedbanks. Trends in Plant Science 24: 783-784. DOI: 10.1016/j.tplants.2019.05.014
6: Wellmann et al. (2023) Maize Grain Germination Is Accompanied by Acidification of the Environment. Agronomy 13: 1819. doi.org/10.3390/agronomy13071819
There are quite reliable Apps for the determination of vascular plants, but what about fungi/mushrooms?
I am looking for a buffer system maintaining a pH range of 6.1 – 6.7 which does not interact with the metabolism of yeast cells included in the reaction solution (positive and negative interaction).
The redox dye resazurin (dark blue) can be reduced in aqueous solution at pH > 6.1 via resorufin (pink) to dihydroresorufin (colourless).
What colour is the resorufin solution at pH < 6.1?
Does anyone have experience with non-destructive seed viability testing?
We use Thermo Scientific Superfrost plus gold coated slides. Why our cryo sections of dry fruits do not stick to the slides?
We were not successful using Toluidine blue staining.
