Justyna Lema-Rumińska

Kazimierz Wielki University in Bydgoszcz (Poland) · Department of Environmental Biology

Cacti are important in agricultural economies and one of the most popular horticultural plant groups. The genus Astrophytum is one of the most valuable and desirable cacti for growers and collectors around the world. By selecting the appropriate breeding methods to induce variations in combination with modern biotechnology tools for rapid change de...

Scutellaria barbata D. Don is an important species for the production of new drugs for the phytopharmaceutical industry due to the presence of valuable secondary metabolites with anticancer properties. However, its cultivation in vitro and in vivo is still not understood, and there is currently no standardized plant material with a high content of...

Background. The Astrophytum and Frailea genus are very important cacti for horticultural production and collectors over the world. The problem is their similarity at the seedling or cutting stage. Additionally many of cactus species are endangered in natural habitats and require protection. The aim of the study was to develop a reliable system of m...

Strawberry and wild strawberry are among the most popular horticultural crops. Due to the development of soilless cultivation systems, the whole-year production of these economically important fruit crops is achievable even in countries with temperate climate. However, the responses of strawberry (Fragaria × ananassa Duch.) and wild strawberry (Fra...

Echinacea purpurea (L.) Moench is a plant species important for the phytopharmaceutical industry and in horticulture. Currently, there is lack of standardized plant material with an increased content of secondary meta-bolites in purple coneflower. The following research meets the expectations of the industry, as new selected lines of purple coneflo...

Chrysanthemum (Chrysanthemum × grandiflorum Ramat./Kitam.) occupies a high position on the gardening market, and its production continues to increase every year. To satisfy the growing needs of the market, new methods of propagation and breeding are being sought. One of the most efficient regenerative methods is somatic embryogenesis (SE), which ca...

The objective of the study has been to determine the usefulness of six different ISSR (Inter Simple Sequence Repeat) primers for the genetic stability in the chrysanthemum plants produced through somatic embryogenesis (SE). The research material consisted of the lines of two cultivars: ‘Lady Salmon’ (LS1-LS5) and ‘Lady Vitroflora’ (LV1-LV5). The pl...

Identification of cultivars is essential both in breeding and to settle cultivar disputes. The purpose of the study has been to examine cultivar identities based on absorption spectra of plant pigments and to confirm a genetic stability with SCoT and RAPD molecular markers in new Polish lines of Chenopodium quinoa Willd. Spectral analysis of pigmen...

The genetic diversity was investigated among two chrysanthemum (Chrysanthemum x grandiflorum Ramat./Kitam.) cultivars ‘Lady Salmon’ and ‘Lady Vitroflora’ and its 15 lines of plants derived from somatic embryos in using ten random amplified polymorphic DNA (RAPD) markers. All primers gave 108 bands with 1218 products from 148.65 to 4391.20 bp in siz...

artykuł popularno-naukowy dotyczący roli biotechnologii w XXI wieku

Kaktusy są po storczykach najchętniej kolekcjonowaną grupą roślin. Ich znaczenie na rynku roślin ozdobnych, a także w farmacji staje się coraz większe. Niestety wiele z nich zagrożonych jest wyginięciem. Z tych względów konieczne jest opracowanie wydajnych metod reprodukcji tych pięknych roślin. Obecnie za najbardziej wydajną metodę rozmnażania uwa...

Abstrakt. Współczesna hodowla i reprodukcja chryzantemy korzysta z osiągnięć biotechnologii. Embriogeneza somatyczna u chryzantemy jest cenną metodą regeneracji roślin z komórek wege-tatywnych. Może mieć zastosowanie w hodowli nowych odmian oraz do mikrorozmnażania od-mian, które nie są chimerami. Indukowane i spontaniczne mutacje to z kolei najczę...

Genetic and phenotypic stability of plants obtained via somatic embryogenesis may be disrupted. The reason can be an indirect regeneration of somatic embryos via callus or a high concentration of growth regulators added at the induction stage of somatic embryos. Somatic embryogenesis of ‘Lady Salmon’ (chimeric) and ‘Lady Vitroflora’ (non-chimeric)...

The use of flower tissue for the in vitro propagation of chrysanthemum (Chrysanthemum x grandiflorum (Ramat.) Kitam.), an ornamental plant, provides a specific and precious explant type for organogenesis (regeneration of adventitious shoots/roots) or somatic embryogenesis. This is of great importance for the breeding of this species. In vitro cultu...

Genetic and phenotypic stability of plants obtained via somatic embryogenesis may be disrupted. The reason can be an indirect regeneration of somatic embryos via callus or a high concentration of growth regulators added at the induction stage of somatic embryos. Somatic embryogenesis of ‘Lady Salmon’ (chimeric) and ‘Lady Vitroflora’ (non-chimeric)...

Leaf fragments from in vitro shoot cultures of 10 chrysanthemum ‘Lady’ group radiomutants were cultured on Murashige and Skoog (MS) medium supplemented with 4 mg l-1 of 2,4-dichlorophenoxyacetic acid (2,4-D) alone or in combination with different concentrations (1, 2 or 5 mg l-1) of kinetin (KIN) or 6-benzylaminopurine (BAP). Indirect somatic embry...

In the past, members of the Cactaceae family were mostly propagated by seeds or vegetatively by cuttings and grafting. Seeds, however, do not guarantee genetic stability and, in the case of some cacti, seeds are difficult to obtain, their germination rates are low, or they need to be stored for a long time in specific light and temperature conditio...

Many chrysanthemum mutants are chimeras built from tissues of a varied genetic composition. Regeneration in vitro of somatic embryos from the whole mutated ligulate florets or only from their small mutated fragments can lead to the separation of chimera components and, as a result, producing a new original cultivar. There was determined the effect...

Having produced the embryos of cactus Copiapoa tenuissima Ritt. forma monstruosa at the globular stage and callus, we investigated the effect of abscisic acid (ABA) in the following concentrations: 0, 0.1, 1, 10, and 100 μ M on successive stages of direct (DSE) and indirect somatic embryogenesis (ISE). In the indirect somatic embryogenesis process...

The colour of florets and leaves, considerably determining the decorative nature of plants, can depend much on the conditions throughout the period of cultivation. The research analysed the occurrence of anthocyanins and carotenoids in ray florets and chlorophylls in leaves in Chrysanthemum × grandiflorum /Ramat./Kitam., ‘Baton Rouge’ grown in the...

Cactus Copiapoa tenuissima Ritt. f. monstruosa is a C. tenuissima Ritt. spontaneous mutant. This form has nearly black epidermis and it has no thorns in areoles, and it is very rare and attractive for collectors. Micropropagation is an attractive method for conservation and propagation of rare species of plants. Somatic embryogenesis is most effici...

Astrophytum asterias (Zucc.) Lem. is a cactus which is among those most desired by producers and collectors all across the world and, at the same time, a species threatened with extinction in the natural environment. Micropropagation techniques can be helpful both in terms of its ex situ protection and its popularisation on the market, thus satisfy...

Flow cytometry was used for analyzing DNA contents of nuclei isolated from in vitro grown somatic embryos, shoots and calli, as well as mammillae of in vivo grown shoots of cactus, Copiapoa tenuissima Ritt. forma monstruosa. Endoreduplication was detected in both in vitro grown somatic embryos, shoots and calli and in mammillae derived from in vivo...

The subject of investigation was Chrysanthemum x grandiflorum (Ramat.) Kitam., ‘Satinbleu’. Leaf explants and internodes were excised from the middle part of the donor sterile plant. Two methods of explant inoculation were applied: explants were placed polarly and horizontally. Modified MS medium (Murashige and Skoog 1962), supplemented with 11.4μM...

Copiapoa tenuissima Ritt f. monstruosa is a spontaneous cacti mutant from Copiapoa tenuissima Ritt from Chile. Monstruosa form has nearly black epidermis and it has no thorns in areoles, and it is very rare and attractive for collectors (Dornig 1976, Graham 1998). There was investigated the induction of direct somatic embryogenesis of Copiapoa ten...

Eleven cultivars of Chrysanthemum × grandiflorum (Ramat.) Kitam.: ‘Richmond’ and its 10 radiomutants, representing the Lady group, were propagated in vitro with shoot tips and leaves as explants. The aim of this study was to investigate if the explant type used for micropropagation affects the genotype and phenotype of chrysanthemums. Plants grown...

Ten mutants and one original cultivar of chrysanthemum (Chrysanthemum x grandiflorum /Ramat./Kitam., syn. Dendranthema grandiflora Tzvelev) representing the Lady Group were analysed for pigment content in the inflorescence by the spectrophotometric method. The respective mutants obtained by ionising radiation differed in their quality and quantity...

Random amplified polymorphic DNA (RAPD) markers were used to study the molecular characterization of 10 new radiomutants of chrysanthemum. The original cultivar ‘Richmond’ differed in genetic distance from its Lady group mutants. The analysis of genetic similarity indices revealed low diversity within the radiomutants. The dendrogram obtained after...

Two groups of chrysanthemum (Dendranthema grandiflora Tzvelev) repre-senting the five cultivars were analysed to define their content of pigments in the inflores-cence with the spectrophotometric method. It was observed that respective cultivars obtained as a result of the ionising radiation dif-fered in their quality and quantity of flavonoids and...