Juan Ivan BravoUniversity of Southern California | USC · School of Gerontology
Juan Ivan Bravo
Bachelor of Science
About
17
Publications
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341
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Additional affiliations
August 2017 - present
February 2017 - July 2017
June 2014 - February 2017
Education
August 2012 - May 2016
Publications
Publications (17)
Long interspersed element 1 (LINE-1; L1) are a family of transposons that occupy ~17% of the human genome. Though a small number of L1 copies remain capable of autonomous transposition, the overwhelming majority of copies are degenerate and immobile. Nevertheless, both mobile and immobile L1s can exert pleiotropic effects (promoting genome instabil...
Long interspersed element 1 (L1) are a family of autonomous, actively mobile transposons that occupy ∼17% of the human genome. The pleiotropic effects L1 induces in host cells—promoting genome instability, inflammation, or cellular senescence—are established, and L1’s associations with aging and aging diseases are widely recognized. However, becaus...
Neutrophils are the most abundant human white blood cell and constitute a first line of defense in the innate immune response. Neutrophils are short-lived cells and thus the impact of organismal aging on neutrophil biology, especially as a function of biological sex, remains poorly understood. Here, we describe a multi-omic resource of mouse primar...
Researchers need in vitro models that mirror the biology of organisms. Primary fibroblasts play essential roles in wound healing and are present in many tissues. They are widely used in studies of cell cycle control, reprogramming, and aging. Though extraction protocols exist, alternatives that maximize use of available resources are useful. Here,...
Many familiar traits in the natural world—from lions’ manes to the longevity of bristlecone pine trees—arose in the distant past, and have long since fixed in their respective species. A key challenge in evolutionary genetics is to figure out how and why species-defining traits have come to be. We used the thermotolerance growth advantage of the ye...
Many familiar traits in the natural world--from lions' manes to the longevity of bristlecone pine trees--arose in the distant past, and have long since fixed in their respective species. A key challenge in evolutionary genetics is to figure out how and why species defining traits have come to be. We used the growth advantage of the yeast Saccharomy...
Our understanding of the molecular regulation of aging and age-related diseases is still in its infancy, requiring in-depth characterization of the molecular landscape shaping these complex phenotypes. Emerging classes of molecules with promise as aging modulators include transposable elements, circRNAs and the mitochondrial transcriptome. Analytic...
Although aging is a conserved phenomenon across evolutionary distant species, aspects of the aging process have been found to differ between males and females of the same species. Indeed, observations across mammalian studies have revealed the existence of longevity and health disparities between sexes, including in humans (i.e. with a female or ma...
In multi-cellular organisms, the control of gene expression is key not only for development, but also for adult cellular homeostasis, and deregulation of gene expression correlates with aging. A key layer in the study of gene regulation mechanisms lies at the level of chromatin: cellular chromatin states (i.e. the ‘epigenome’) can tune transcriptio...
In multi-cellular organisms, the control of gene expression is key not only for development, but also for adult cellular homeostasis, and deregulation of gene expression correlates with aging. A key layer in the study of gene regulation mechanisms lies at the level of chromatin: cellular chromatin states (i.e. the 'epigenome') can tune transcriptio...
The microbiomes of plants are critical to host physiology and development. Microbes are attracted to the rhizosphere due to massive secretion of plant photosynthates from roots. Microorganisms that successfully join the rhizosphere community from bulk soil have access to more abundant and diverse molecules, producing a highly competitive and select...
The manipulation and engineering of microbiomes could lead to improved human health, environmental sustainability, and agricultural productivity. However, microbiomes have proven difficult to alter in predictable ways, and their emergent properties are poorly understood. The history of biology has demonstrated the power of model systems to understa...
In multi-cellular organisms, the control of gene expression is key not only for development, but also for adult cellular homeostasis, and gene expression has been observed to be deregulated with aging. In this review, we discuss the current knowledge on the transcriptional alterations that have been described to occur with age in metazoans. First,...
Plants expend significant resources to select and maintain rhizosphere communities that benefit their growth and protect them from pathogens. A better understanding of assembly and function of rhizosphere microbial communities will provide new avenues for improving crop production. Secretion of antibiotics is one means by which bacteria interact wi...
The quest to manipulate microbiomes has intensified, but many microbial communities have proven recalcitrant to sustained change. Developing model communities amenable to genetic dissection will underpin successful strategies for shaping microbiomes by advancing understanding of community interactions. We developed a model community with representa...
Pseudomonas koreensis CI12 was coisolated with Bacillus cereus from a root of a soybean plant grown in a field in Arlington, WI. Here, we report the draft genome sequence of P. koreensis CI12 obtained by Illumina sequencing.
Flavobacterium johnsoniae CI04 was coisolated with Bacillus cereus from a root of a field-grown soybean plant in Arlington, WI, and selected as a model for studying commensalism between members of the Cytophaga-Flavobacterium-Bacteroides group and B. cereus . Here we report the draft genome sequence of F. johnsoniae CI04 obtained by Illumina sequen...
Questions
Question (1)
I'm trying to overexpress the Renilla luciferase gene with a minimal CMV promoter, and I've noticed that expression is relatively high when I transfect into HEK293T cells, but relatively low when I transfect that same plasmid into IMR-90s. That is, when I run a luciferase assay with the two cell types, I get 1/20 if not less luminescent signal in the fibroblasts compared to 293Ts. I'm curious if anyone has noticed something similar?
Thanks!
