José Carlos Lorenzo Feijoo

University of Ciego de Ávila · Bioplantas

Gerbera represents one of the top five most important traditional cut flowers. A major factor influencing the success of the cut flower industry is vase life which in turn has a significant influence on consumer preference. Therefore, the present study considered the effect of silver nanoparticles (AgNPs) supplied in temporary immersion bioreactors...

Teramnus labialis (of the Fabaceae family) is an under-utilized legume with immense potential to improve agricultural soils (through atmospheric nitrogen fxation) and for use as forage for livestock. Despite its pantropical distribution, only a limited number of studies is available to characterize germplasm of this genus. In Cuba, two cultivars ar...

Teramnus labialis (of the Fabaceae family) is an under-utilized legume with immense potential to improve agricultural soils (through atmospheric nitrogen fixation) and for use as forage for livestock. Despite its pantropical distribution, only a limited number of studies is available to characterize germplasm of this genus. In Cuba, two cultivars a...

Cicer arietinum L. is a major food legume across the globe. However, the yield of legume crops appears to have reached a plateau in developing countries where yield is often impacted by poor crop establishment. Therefore, seed physiological characteristics (specific to the cultivars/landraces adapted to various regions of the world) and their impac...

BACKGROUND: Cryopreservation currently represents the most suitable strategy for the long-term conservation of plant germplasm. While much effort has focused on the development of protocols to enable successful cryostorage, there are few, if any reports, that consider the effect of cryogenic temperatures on the phytohormone status of the seed and d...

Consid-ering the scarcity of information on the characterization of below-ground attributes of pineapple genetically modified plants, as well as the importance of the rhizosphere in plant growth, the present study investigated the chemical charac- terization of the roots of pineapple transgenic plants as part of an ongoing biosafety assessment of t...

BACKGROUND: N. wightii (Leguminosae) is valued as a cover crop and as a potential source of protein in food insecure countries. However, plantlet establishment is limited by physical dormancy. Our previous work has shown that exposure of N. wightii seeds to cryogenic temperatures is able to overcome physical dormancy. OBJECTIVE: The current study i...

Studies on the morpho-physiology of cryo-derived pineapple plants after acclimatization have been quite limited. Therefore, in the present study, the morpho-anatomical and physiological characteristics of cryo-derived Ananas comosus var. comosus ‘MD-2’ plants after acclimatization were investigated. Plants obtained from cryopreserved and non-cryopr...

This work describes the biochemical responses of water hyacinth (Eichhornia crassipes) plants to salinity stress under controlled conditions. Low to medium levels of necrosis were observed with plant fresh weight remaining stable across all treatments. Investigation of lipid peroxidation products revealed that levels of malondialdehyde were signifi...

The present study provides the first report on the cryopreservation of seeds of Tamarindus indica. Intact seeds were dehydrated to a moisture content of 6% (fresh weight basis) before immersion in liquid nitrogen. Following retrieval from cryogenic temperatures, it was apparent that plants regenerated from cryostored seeds displayed delayed germina...

Se estudiaron siete lagunas artificiales de agua dulce (L1 – L7) en el municipo de Ciego de Ávila (Cuba), los cuales se caracterizaron según criterios genéticos, hidrológicos y morfológicos. Todas son lagunas, de origen antrópico, relativamente recientes. Una de ellas es semipermanente (L.1) y las restantes son permanentes. A partir de la identific...

01 CURSO BIOESTADÍSTICA Y METODOLOGÍA DE LA INVESTIGACIÓN (BIOTECNOLOGÍA DE PLANTAS)

02 CURSO BIOESTADÍSTICA Y METODOLOGÍA DE LA INVESTIGACIÓN (BIOTECNOLOGÍA DE PLANTAS; MONOFACTORIALES CON 2 NIVELES)

01 CURSO BIOESTADÍSTICA Y METODOLOGÍA DE LA INVESTIGACIÓN (BIOTECNOLOGÍA DE PLANTAS; INTRODUCCION)

02 CURSO BIOESTADÍSTICA Y METODOLOGÍA DE LA INVESTIGACIÓN (BIOTECNOLOGÍA DE PLANTAS; MONOFACTORIALES CON 2 NIVELES)

09 CURSO BIOESTADÍSTICA Y METODOLOGÍA DE LA INVESTIGACIÓN (BIOTECNOLOGÍA DE PLANTAS; PORCENTAJES)

04 CURSO BIOESTADÍSTICA Y METODOLOGÍA DE LA INVESTIGACIÓN (BIOTECNOLOGÍA DE PLANTAS; EXPERIMENTOS BIFACTORIALES)

Teramnus labialis (Leguminosae) is an underutilized species that has the potential to play an important role in diverse agro-ecosystems as a protein supplement for animal feed and a cover crop to conserve soil health. However, the establishment of this plant is difficult due to low levels of seed germination as a consequence of seed dormancy. The p...

S. rebaudiana is a sought after sweetener because of its low-calorie properties. However, the supply of suitable quantities of high quality propagation material is limited by inefficient propagation methods using conventional strategies. In vitro techniques combined with nanotechnology tools offer an attractive alternative not only for improved pro...

NORMAS Y SUGERENCIAS PARA DESARROLLAR LA INVESTIGACIÓN Y LA TESIS DE DOCTORADO EN CIENCIAS AGRÍCOLAS. Este compendio (borrador 1) fue elaborado por Dr. José Carlos Lorenzo Feijoo y es un resumen del contenido que él explica en el curso Bioestadística y Metodología de la Investigación de la Maestría en Agrobiotecnología. Jefe, Departamento para Me...

Cicer arietinum L. is a major food legume across the globe. However, the yield of legume crops appears to have reached a plateau in developing countries where yield is often impacted by poor crop establishment. Therefore, seed physiological characteristics (specific to the cultivars/landraces adapted to various regions of the world) and their impac...

In previous works, a method was developed for the differentiation at the foliar level of the response of banana plants to Fusarium race 1 wilt, which constitutes a useful tool to increase the efficiency of resistance selection in ex vitro conditions. The objective of this work was to evaluate resistance to this disease in new banana clones using th...

S. rebaudiana is a sought after sweetener because of its low-calorie properties. However, the supply of suitable quantities of high quality propagation material is limited by inefficient propagation methods using conventional strategies. In vitro techniques combined with nanotechnology tools offer an attractive alternative not only for improved pro...

Background: Swietenia mahagoni wood is one of the most valuable in world trade and, as a result, natural populations have been decimated due to unsustainable harvesting. The decline in natural population levels is being exacerbated by climate change. In order to ensure the preservation of valuable genotypes, there is an urgent need to develop stra...

Pineapple production is under threat due to climate change. Hence, it is critical to maintain a diverse gene pool to develop improved cultivars. Cryopreservation represents a viable option for the long-term storage of germplasm with minimal space requirements and maintenance. Methods have been developed for cryopreservation of pineapple shoot tips...

Background: Trees within the Calophyllum genus are multi-use trees that produce valuable wood, phytochemicals with a range of biological activities, and seed oil as a source of biodiesel. As a consequence of climate change, there is a need to develop strategies to preserve valuable plant genetic resources. Cryopreservation represents the most suit...

In Peru, potato cultivation represents 25% of the agricultural gross domestic product yet only 0.2% of the agamic seed used is from certified sources. The use of temporary immersion bioreactors (TIB) has improved the quality of microtubers micropropagated along with savings in costs of production. The current study investigated the agronomic perfor...

Projections of warming temperatures as a consequence of climate change will have impacts on food production globally. This heat stress coupled with increased solar radiation, will affect the well-being of livestock. In many regions of the world, local breeds are reared as they demonstrate improved tolerance to local conditions resulting in minimal...

A total of twenty-four breeding ewes from Pelibuey breed were used, divided into two treatments of 12 animals each, to determine the effect of natural shade on the behavior of these animals during grazing. In treatment one, they grazed in the system with natural shade and in treatment two, without shade. Each treatment included the three physiologi...

In order to determine the typologies of sheep production systems in Ciego de Ávila province, the statistical model of impact measuring (SMIM) was applied, which combines the main components analysis with the hierarchical cluster method. An information matrix of 296 sheep farmers was used to define groups of farms. The main component analysis explai...

One of the projected effects of climate change is a reduction in rainfall in certain regions of the world. Hence, the agricultural and livestock sectors will have to cope with increasing incidences of water shortage while still maintaining productivity levels to feed an ever increasing global population. This short communication reports on the effe...

Although pineapple (Ananas comosus var. comosus) shoot tips have been cryopreserved but the possible effect of this process at the molecular level has not been studied. This communication describes the growth (plant fresh and dry weights; stem height; leaf length, width and area; and stem base diameter) and the Inter Simple Sequence Repeat (ISSR) a...

This current work describes the response of Pistia stratiotes (water lettuce) to moderate saline stress under controlled conditions. Plant biomass as well as the levels of aldehydes, phenolics, chlorophylls and carotenoids was evaluated after a week of stress. Following the first experiment (up to 80mM NaCl), high levels of mortality were observed....

Pepper seeds are slow to germinate and emergence is often non-uniform and incomplete, reducing gains from this cash crop. This study investigated the effects of pineapple stem- derived protease (stem bromelain) based priming on pepper seed germination in relation to reserve mobilization (specifically, proteins and amino acids), germination, emergen...

ARTÍCULO DE INVESTIGACIÓN Fitotecnología para la recuperación de agroecosistemas contaminados con metales pesados por desechos industriales Phytotechnology for the recovery of agroecosystems contaminated with metals heavy for industrial waste RESUMEN La contaminación industrial de los suelos, con metales pesados afecta la inocuidad alimentaria, amb...

Sheep farming, and the income generated from this endeavor, contributes significantly to the global economy and rural livelihoods. Therefore, it is vital to maintain the productivity of this industry in the face of changing climate patterns. In the context of sheep farming, animals are exposed to a higher heat load as global temperatures are increa...

Maize represents a staple food crop and is the second most important agricultural commodity globally. Considering the important role of maize for food security, the long-term conservation of valuable germplasm is critical to ensure that high levels of genetic diversity are available for breeding superior cultivars to face future challenges. Cryopre...

One of the projected effects of climate change is a reduction in rainfall in certain regions of the world. Hence, the agricultural and livestock sectors will have to cope with increasing incidences of water shortage whilst still maintaining productivity levels to feed an ever increasing global population. This short communication reports on the eff...

Contamination of water through salinization will affect the well-being of livestock. Therefore, it is necessary to understand how animals respond to increasing levels of salts in their water supply. This short communication describes the effects of a two-week salinity stress (0, 6, 12 g L− 1 NaCl) on Pelibuey sheep. The parameters measured in respo...

Potato cultivation is limited by a lack of access to quality propagation material. The application of micropropagation techniques combined with the diagnosis and sanitation of the main pathogens of the crop, has contributed to increased production efficiencies. In this regard, the use of temporary immersion bioreactors (TIBs) has improved the quali...

Pineapple is the third most important tropical fruit traded after banana and mango. In recent years, pineapple producers have faced production challenges due to unpredictable weather patterns as a consequence of climate change. In order to develop new genotypes with improved tolerance to these biotic and abiotic stresses, it is necessary to have ac...

Pepper fruit has a great agronomic, nutritional and commercial value given that it is rich in antioxidant compounds such as carotenes, and vitamins C and E. However, pepper seeds are slow to germinate and emergence is often non-uniform and incomplete. Seed priming and invigoration treatments have been explored for a number of pepper varieties but s...

Livestock farming with sheep represents an important income stream. With climate change, domestic sheep are being exposed to heat stress which can have adverse effects on growth. Here, data regarding sheep behaviour in response to high temperature stress was analysed using the Euclidean distance method to integrate all variables into a single repre...

DOI: 10.15835/nsb13110859 Livestock farming with sheep represents an important income stream. With climate change, domestic sheep are being exposed to heat stress which can have adverse effects on growth. Here, data regarding sheep behaviour in response to high temperature stress was analysed using the Euclidean distance method to integrate all va...

Background: Teramnus labialis (L.f.) Spreng is a potentially important legume species, and can be used as an animal feed and to enhance soil physicochemical characteristics. Despite the biological and agricultural importance, the low availability of seeds, their small size and the low percentage germination limit their large-scale use by farmers....

Teramnus labialis (L.f.) Spreng is a potentially important legume species, and can be used as an animal feed and to enhance soil physicochemical characteristics. Despite the biological and agricultural importance, the low availability of seeds, their small size and the low percentage germination limit their large-scale use by farmers. We previously...

The production of pineapple, one of the most economically important tropical fruits globally, is increasingly being threatened by biotic and abiotic factors. Germplasm conservation of pineapple is essential for sustaining breeding programs, and in this regard, cryopreservation is thought to be the best long-term storage option. This short communica...

Maize represents a staple food crop and is the second most important agricultural commodity globally. Considering the important role of maize for food security, the long-term conservation of valuable germplasm is critical to ensure that high levels of genetic diversity are available for breeding superior cultivars to face future challenges. Cryopre...

In terms of tropical fruit production, pineapple ranks as one of the major commercially grown fruit crops globally. Our previous research led to the development of a technique suitable for genetic modification of pineapple. The transgenic plants generated were characterized following hardening-off and during the subsequent eight years of in situ gr...

Pineapple is an economically important tropical fruit crop, but the lack of adequate planting material limits its productivity. A range of micropropagation protocols has been developed over the years to address this shortfall. Still, the final stage of micropropagation, i.e. acclimatisation, remains a challenge as pineapple plantlets grow very slow...

Food security concerns are emerging in many parts of the world due to climate change, disease, and diminishing areas of arable land. Risks to food security can be alleviated by advances in plant biotechnology such as cryopreservation (usually at −196 °C in liquid nitrogen (LN)). While seed cryopreservation methods are being developed for a number o...

Teramnus labialis (L.f.) Spreng is a legume that plays an important role in mixed agroecosystems, due to its use as animal feed and its role in restoring soil nitrogen levels. However, its wide-scale use in agriculture and revegetation efforts is limited due to its physically dormant seeds. In our previous study on the effects of seed cryopreservat...

En trabajos previos se desarrolló un método para la diferenciación a nivel foliar de la resistencia de cultivares de banano a la marchitez por Fusarium . El presente estudio se realizó con el objetivo de evaluar el método para la diferenciación rápida a la enfermedad mediante la utilización del filtrado del cultivo de Fusarium oxysporum f. sp. cube...

Polynomial regression analysis is a powerful statistical tool used to describe non-linear relationships. The goodness-of-fit of this model is assessed by evaluating the coefficient of determination (R2). An R2 value closer to 1 is indicative of a strong correlation between the two under consideration. While polynomial regression analysis has been w...

Seed heteromorphism can influence germination and ultimately seedling establishment, particularly in disturbed habitats.This study compared seed and seedling traits across three distinctly colored seed morphs (viz. light-brown, brown and dark-brown) of the forage legume,Teramnus labialis. Brown seeds were of the best quality (i.e. un-parasitized, f...

Seed heteromorphism can influence germination and ultimately seedling establishment, particularly in disturbed habitats.This study compared seed and seedling traits across three distinctly colored seed morphs (viz. light-brown, brown and dark-brown) of the forage legume,Teramnus labialis. Brown seeds were of the best quality (i.e. un-parasitized, f...

Pineapple is among the most important fruits worldwide. However, the narrow genetic base of current commercial cultivars renders the industry at risk from environmental and other biological threats. Hence, there is a need to develop improved cultivars with tolerance to biotic and abiotic stresses. Our prior research resulted in the establishment of...

Pineapple is among the most important fruits worldwide. However, the narrow genetic base of current commercial cultivars renders the industry at risk from environmental and other biological threats. Hence, there is a need to develop improved cultivars with tolerance to biotic and abiotic stresses. Our prior research resulted in the establishment of...

Teramnuslabialis (L.f.) Spreng is a legume that plays an important role in agriculture, due to its use as animal feed and its role as an enhancer of soil physicochemical conditions. However, given previous reports on the effects of seed cryopreservation on seedling vigor and biochemistry in a number of species, the present study looked at the effec...

One of the most widespread legume species in the world is N.wightii, which is used as a cover crop for animal feed and has positive environmental impacts. However, plant establishment is difficult due to the dormancy of seeds. There are several methods available to break dormancy, with exposure tolow temperature being the strategy most frequently u...

One of the most widespread legume species in the world is N. wightii, which is used as a cover crop for animal feed and has positive environmental impacts. However, plant establishment is difficult due to the dormancy of seeds. There are several methods available to break dormancy, with exposure to low temperature being the strategy most frequently...

Knowing the mechanisms that operate under water stress in commercial crops, particularly those that can affect productivity, such as phenolic or cell wall metabolism, is becoming increasingly important in a scenario of global climate change. However, our understanding of how to analyse statistically the relationships between these commonly used bio...

Conservation of pineapple (Ananas comosus L. Merr.) genetic resources – including cryopreservation in liquid N2 at -196°C – is essential for future breeding programmes to develop new varieties with improved agronomic performance. However, the potentially deleterious effects of cryopreservation on subsequent plant regrowth should be evaluated before...

Biojas® is a fermented broth of Lasiodiplodia theobromae, a jasmonic acid–producing fungus characterized as a plant growth regulator and as biological control of phytopathogenic microorganisms and pests. The present work describes the use of Biojas® in in vitro culture of pineapple. On the other hand, plant scientists usually record multiple indica...

Temporary immersion bioreactors (TIBs) have been shown to be useful for studying plant stress physiology and inducing chemical mutagenesis. This study describes the effects of exposure to NaCl (salt stress) and mannitol (osmotic stress) within TIB on sugarcane mineral levels in vitro. Shoots were exposed to concentration of NaCl (89.4 mM) and manni...

In light of climate change and risks of food insecurity, it is becoming increasingly important to preserve plant germplasm in genebanks. Storage of seeds, particularly via cryopreservation, is one of the most proficient methods for ex situ plant germplasm conservation. Whilst seed cryo-banking can have little, to no, or even beneficial effects on s...

In light of climate change and risks of food insecurity it is becoming increasingly important to preserve plant germplasm in genebanks. Storage of seeds, particularly via cryopreservation, is one of the most proficient methods for ex situ plant germplasm conservation. Whilst seed cryo-banking can have little, to no, or even beneficial effects on su...

Chemical mutagens such as sodium azide (NaN3) have been widely used to increase genetic variability in crops but the undirected mutations induced can have undesirable effects, which need to be characterized. This study investigated the effects of in vitro NaN3 (0 - 0.45 mM) exposure (30 d) on the micropropagation of sugarcane within temporary immer...

BACKGROUND: Teramnus labialis is an herbaceous legume that serves as a source of carbohydrates and proteins for animals and humans, and is valued for its nitrogen contribution to soil. The benefits of this species are, however, limited by low seed availability, small seed size and low in situ seed germination levels, due to physical dormancy. Cryos...

Climate change poses risks to both wild and crop plant biodiversity, which can be mitigated by cryopreservation (usually at-196 °C in liquid nitrogen [LN]) of crop germplasm. Cryopreservation is widely regarded as a reliable method for the ex situ conservation of plant genetic resources but its effects on subsequent field performance of popular cro...

One of the most widely used approaches to study cytokinin mode of action involves the use of cytokinin antagonists (anticytokinins). Despite this, few studies have compared the chemical structure of cytokinins and anticytokinins. This formed the focus of the present study, which identified a range of molecular descriptors differentiating four cytok...

Globally, production of pineapple fruits reached more than 25 million tons in 2013. Therefore, many scientists are searching for new varieties and ways for the conservation of pineapple genetic resources. Cryoconservation in liquid nitrogen has been described as a suitable technology in many plant species. However, its potential effects in the subs...

15 Sodium azide (NaN 3) is widely used to induce mutagenesis within in vitro plant 16 systems. However, since this mutagenesis is undirected, its unintended effects demand 17 characterization. This study investigated the mutagenic effects of sodium azide (0-18 0.45 mM) on selected growth (shoot multiplication rate and shoot cluster fresh weight) 19...

BACKGROUND: Teramnus labialis is an herbaceous legume that serves as a source of carbohydrates and proteins for animals and humans, and is valued for its nitrogen contribution to soil. The benefits of this species are, however, limited by low seed availability, small seed size and low in situ seed germination levels, due to physical dormancy. Cryos...

In this paper, we investigated if the differences consistently noted in survival and plantlet production between cryopreserved and non-cryopreserved, control sugarcane embryogenic calluses were related to modifications induced during cryopreservation in the structural and functional integrity of cell membranes. For this, the evolution of electrolyt...

Molecular descriptors to differentiate four gibberellins and seven antigibberellins were studied. DRAGON software and CambridgeSoft ChemOffice were used to calculate 212 descriptors. Of them, 48 showed statistically significant differences between gibberellins and antigibberellins which can be summarized as follows. Gibberellins contain as average...

Sodium azide (NaN3) is widely used to induce mutagenesis within in vitro plant systems. However, since this mutagenesis is undirected, its unintended effects demand characterization. This study investigated the mutagenic effects of sodium azide (0 - 0.45 mM) on selected growth (shoot multiplication rate and shoot cluster fresh weight) and biochemic...

BACKGROUND: Cryo-preservation of plant materials in liquid nitrogen (LN) has been described as a suitable technology to conserve genetic resources of several species. However, the potential effects of LN in the subsequent plant growth in the field should be studied before large-scale implementation of cryopreserved germplasm banks. OBJECTIVE: This...

This study compares 212 molecular descriptors of four auxins (indolebutyric acid; indoleacetic acid; 2,4-dichlorophenoxyacetic acid; 1-naphthaleneacetic acid), three auxin transport inhibitors [2-(naphthalen-1-ylcarbamoyl)benzoic acid; 2,3,5-triiodobenzoic acid; 9-hydroxy-9H-fluorene-9-carboxylic acid], and five competitors for auxin receptors [2-(...

This study compares 212 molecular descriptors of four auxins (indolebutyric acid; indoleacetic acid; 2,4-dichlorophenoxyacetic acid; 1-naphthaleneacetic acid), three auxin transport inhibitors [2-(naphthalen-1-ylcarbamoyl)benzoic acid; 2,3,5-triiodobenzoic acid; 9-hydroxy-9H-fluorene-9-carboxylic acid], and five competitors for auxin receptors [2-(...

BACKGROUND: Cryo-preservation of plant materials in liquid nitrogen (LN) has been described as a suitable technology to conserve genetic resources of several species. However, the potential effects of LN in the subsequent plant growth in the field should be studied before large-scale implementation of cryopreserved germplasm banks. OBJECTIVE: This...

Chemical mutagens, such as sodium azide, have attracted the interest of plant breeders. Azide creates DNA point mutations and affects plant growth and development, disturbs metabolic activity and inhibits protein and DNA replication, whereas mannitol is used to simulate drought stresses in tissue culture. To identify biochemical markers for stress...

Chemical mutagens, such as sodium azide, have attracted the interest of plant breeders. Azide creates DNA point mutations and affects plant growth and development, disturbs metabolic activity and inhibits protein and DNA replication, whereas mannitol is used to simulate drought stresses in tissue culture. To identify biochemical markers for stress...

We have identified those molecular descriptors differentiating four auxins, four cytokinins and four gibberellins. DRAGON software (version 5.5, 2007) and CambridgeSoft ChemOffice (version 12, 2010) including ChemDraw and Chem3D were used to calculate 212 molecular descriptors. Only 49 descriptors showed statistically significant differences among...

We recorded the crypreservation effects (direct immersion) on various parameters of early germination stages of maize seeds (0, 7 and 14 days). Percentages of germination; fresh mass of different seedling parts; levels of chlorophyll pigments (a, b); carotenoids; malondialdehyde; other aldehydes; phenolics (cell wall-linked, free) and proteins were...

In light of climate change and risks of food insecurity, it is becoming increasingly important to preserve plant germplasm in genebanks. Storage of seeds, particularly via cryopreservation, is one of the most proficient methods for ex situ plant germplasm conservation. Whilst seed cryo-banking can have little, to no, or even beneficial effects on s...

Potato is an important food and cash crop in Perú. Its production is limited due to seed quality, and other biotic and abiotic factors. Here we explore a new alternative for potato pre-basic seed production named temporary immersion bioreactor (TIB). Potato microtubers production using TIB comprises two phases: multiplication and microtuberization....

Plant scientists usually record several indicators in their abiotic factor experiments. The common statistical management involves univariate analyses. Such analyses generally create a split picture of the effects of experimental treatments since each indicator is addressed independently. The Euclidean distance combined with the information of the...