João Miguel Marques dos Santos

Faculty of Medicine - University of Coimbra · Institute of Endodontics

Introduction: The purpose of this study was to compare tooth discoloration that occurs in teeth filled with ProRoot MTA (DENTSPLY Tulsa Dental Specialties, Tulsa, OK) or Biodentine (Septodont, Saint Maur des Fossés, France) over the course of 1 year. Methods: Twenty-eight intact premolars were resected 2 mm apical to the cementoenamel junction a...

Aim. The aim of this comprehensive literature review was to address the question: Does photodynamic therapy (PDT) improve root canal disinfection through significant bacterial reduction in the root canal system? Methodology. A comprehensive narrative literature review was performed to compare PDT effect with sodium hypochlorite as the comparative c...

The aim of this in vitro study was to assess the substantive antimicrobial activity of different medicaments in human root dentin. Canals of 98 roots were enlarged to standard size and medicated for 7 days with the following: (1) 2% chlorhexidine (CHX) gel, (2) 0.2% CHX gel, (3) 2% CHX solution, (4) Ca(OH)(2), (5) Ca(OH)(2)+ 0.2% CHX gel, (6) 2% CH...

Placement of orifice plugs has been suggested to augment the seal of conventional root canal fillings. This study assessed in vivo the efficacy of white mineral trioxide aggregate (MTA) plugs in preventing periapical inflammation subsequent to coronal inoculation of root-filled teeth. The two-rooted mandibular premolars of six beagle dogs were conv...

Bone defects may be a result of different pathologies and represent a challenge in different fields of dentistry. Techniques for the correction of bone defects involving the use of several types of grafts have been proposed. This study evaluated bone repair in rat tibiae after surgically created critical-size defects were filled with β-tricalcium p...

Objectives: This clinical study aimed to evaluate and compare the diagnostic accuracy of intraoral periapical radiography (PR) and cone beam computed tomography (CBCT) in detecting external apical root resorption (EARR) in orthodontic patients during the retention phase. Methods: The research involved 41 Caucasian patients who had undergone compreh...

Background and Objectives: Regenerative dentistry aims to regenerate the pulp–dentin complex and restore those of its functions that have become compromised by pulp injury and/or inflammation. Scaffold-based techniques are a regeneration strategy that replicate a biological environment by utilizing a suitable scaffold, which is considered crucial f...

Biocompatibility and biomineralization of root canal dressings are important requirements for periapical healing. This study evaluated the inflammatory response, biomineralization and tissue repair by collagen fiber maturation in the subcutaneous tissue of rats. Eighteen Wistar rats (n = 6) received subcutaneous implants: calcium hydroxide + propyl...

Objective: This review aims to assess structural, chemical, and mechanical properties of coronal dentin after endodontic irrigation. Materials and Methods: Reporting followed the PRISMA extension for scoping reviews. An electronic search was carried out in PubMed, Embase, and Cochrane Library. Records filtered by language and published up to Novem...

Disruptions in the MBD5 gene have been linked with an array of clinical features such as global developmental delay, intellectual disability, autistic-like symptoms, and seizures, through unclear mechanisms. MBD5 haploinsufficiency has been associated with the disruption of primary cilium-related processes during early cortical development, and thi...

Oral mucositis is a common and debilitating side effect induced by stem cell transplantation that is experienced by cancer patients undergoing chemotherapy or radiation therapy. This condition involves inflammation and ulceration of the oral mucosa, leading to pain, difficulty with eating and speaking, and an increased risk of infections. Mucositis...

Nanocarriers (NCs) are dynamic nanovehicles used to transport bioactive derivatives like therapeutical formulations, drugs and/or dyes. The current review assists in understanding the mechanism of action of several recent developed NCs with antimicrobial purposes. Here, nine NCs varieties are portrayed with focus on nineteen approaches that are ful...

Objective: To evaluate the effectiveness of supplemental photodynamic therapy for improving the bacterial removal and the levels of lipopolysaccharide (LPS) and lipoteichoic acid (LTA) by conducting a clinical trial. Methodology: Twenty-four root canals with pulp necrosis and periapical lesion were selected and randomly divided into conventional...

Intentional replantation (IR) may offer a solution for persistent periapical lesions associated with endodontically treated teeth. A 35-year-old male patient presented with pain associated with the left mandibular second molar and hypoesthesia. Upon clinical examination, increased probing pocket depth in the mid-buccal surface was detected. Cone be...

Introduction: This study aims to evaluate the long-term outcome of sixteen permanent maxillary central incisors with nonvital pulps and open apices treated with apexification and corono-radicular adhesive restorations, within a follow-up span of 5 to 22 years. Methods: Fourteen patients providing a total of sixteen teeth treated with MTA (n = 12...

The ChAdOx1 nCoV-19 vector vaccine (Vaxzevria, AstraZeneca, Cambridge, UK) was developed at Oxford University and is considered safe for the administration in lactating mothers. Nevertheless, as a novel vaccine, there are gaps in the knowledge regarding possible adverse events in breastfeeding infants of vaccinated mothers. This case report provide...

Objective: To compare in vitro irrigation systems’ efficacy in removing calcium hydroxide paste (Ca(OH)2) in single-root teeth. Methods: Sixty permanent teeth were prepared using ProTaper® rotatory instruments, up to F3. Specimens were split longitudinally into two halves, and two grooves located in the apical and coronal zones were created. Ca(OH...

Objectives To compare the regenerative properties of human stem cells of the apical papilla (SCAPs) embedded in a platelet-rich plasma (PRP) scaffold, when implanted in vivo using an organotypic model composed of human root segments, with or without the presence of the bioactive cements – ProRoot MTA or Biodentine.Material and methodsSCAPs were iso...

Background and Objectives: Endodontic microsurgery (EMS) aims to eradicate the sources of infection once the apical root resection removes most of the infected anatomical structures and repairs potential procedural errors in the apical region. An endodontic-periodontal lesion yields a pathological communication between the pulp and the periodontium...

Citation: Paulo, C.R.; Marques, J.A.; Sequeira, D.B.; Diogo, P.; Paiva, R.; Palma, P.J.; Santos, J.M. Influence of Blood Contamination on Push-Out Bond Strength of Three Calcium Silicate-Based Materials to Root Dentin. Appl. Sci. 2021, 11, 6849.

Introduction: This study aims to evaluate the impact of pre-operative pulp inflammation on the histologic outcome of full pulpotomy performed in mature permanent posterior teeth, using four different biomaterials.; METHODS: Five beagle dogs (providing a total of 120 roots) were selected. Dentin exposure was performed in teeth from second and third...

Background and Objectives: Symptomatic irreversible pulpitis in permanent mature teeth is a common indication for nonsurgical root canal treatment (NSRCT), but contemporary studies have reported on vital pulp therapy (VPT) applied in such teeth as a less invasive treatment. This systematic review assessed the outcomes of VPT, including partial and...

Scientific paper drawn up by the COVID-19 MD group aiming the dissemination of the knowledge about the clinical practice in Dentistry in the context of COVID-19.

Atualização Trabalho científico elaborado pelo grupo COVID-19 MD objetivando a disseminação do conhecimento sobre o exercício clínico em Medicina Dentária no contexto da COVID-19.

Atualização Trabalho científico elaborado pelo grupo COVID-19 MD para a disseminação do conhecimento sobre o exercício clínico em Medicina Dentária no contexto da COVID-19.

Scientific paper drawn up by the COVID-19 MD group aiming the dissemination of the knowledge about the clinical practice in Dentistry in the context of COVID-19.

COVID-19 | Pediatric Dentistry Extension Clinical Guidance Dentistry | Update A scientific paper was drawn up by the COVID-19 MD group aiming at the dissemination of knowledge about the clinical practice in Dentistry in the context of COVID-19.

COVID-19 | Orthodontics Extension Clinical Guidelines Dentistry | Update Scientific paper drawn up by the COVID-19 MD group aiming the dissemination of the knowledge about the clinical practice in dentistry in the context of COVID-19.

Versão resumo da Atualização Trabalho científico elaborado pelo grupo COVID-19 MD para a disseminação do conhecimento sobre o exercício clínico em medicina dentária no contexto da COVID-19.

Atualização do Trabalho científico elaborado pelo grupo COVID-19 MD para a disseminação do conhecimento sobre o exercício clínico em medicina dentária no contexto da COVID-19.

Atualização Trabalho científico elaborado pelo grupo COVID-19 MD para a disseminação do conhecimento sobre o exercício clínico em medicina dentária no contexto da COVID-19.

COVID-19 | Orthodontics Extension Clinical Guidelines Dentistry | Short version of Update Scientific paper drawn up by the COVID-19 MD group aiming the dissemination of the knowledge about the clinical practice in dentistry in the context of COVID-19.

Objectives The aim of this study was to evaluate the shear bond strength inherent to immediate and delayed definitive composite resin restorations, over three different biomaterials. Methods Fifty-four aluminium blocks were randomly assigned to six experimental groups (n = 9) according to the biomaterial (Biodentine, TotalFill BC Putty, or PCM) and...

Citation: Santos, J.M.; Coelho, C.M.; Sequeira, D.B.; Marques, J.A.; Pereira, J.F.; Sousa, V.; Palma, P.J.; Santos, A.C. Subcutaneous Implantation Assessment of New Calcium-Silicate Based Sealer for Warm Obturation. Biomedicines 2021, 9, 24. https://doi.org/10.3390/biomedicines9010024

Objetivos: Avaliar a biocompatibilidade de dois novos cimentos endodônticos à base de silicato de cálcio, TotalFill BC Sealer e TotalFill BC Sealer HiFlow (FKG, La Chaux-des-Fonds, Switzerland), através de implantação subcutânea. Materiais e métodos: A implantação subcutânea foi reali-zada em 16 ratos Wistar. Em cada um dos animais foram implantado...

The aim of this study is to compare root-end preparation performed with two different ultrasonic tips-CVDentus and NSK-and respective time requirements. After root-end resection, 32 teeth were randomly divided in two groups, according to the ultrasonic tip used for root-end preparation. Preparation time was recorded. Photomicrographs were taken to...

Background and objectives: The long-term outcome of endodontic microsurgery (EMS) performed on root-filled teeth affected by post-treatment apical periodontitis (AP) has been a matter of debate, re-launched by the introduction of novel root-end filling materials which have been proven to improve the short-term outcome of EMS. The purpose of this sy...

The aim of the present ex vivo study was to assess and compare coronal discoloration induced by four endodontic biomaterials used in regenerative endodontic procedures (REPs). Root resection was executed horizontally, 2 mm apical to the cementoenamel junction, in all fifty-four teeth. After accessing the pulp chamber, specimens were randomly divide...

Synopsis Em Dezembro de 2019, o mundo assistiu à emergência de uma zoonose provocada por um novo tipo de Corona vírus o SARS CoV 2. Esta infeção rapidamente evoluiu para uma disseminação pandémica com consequências pesadas em todos os planos civilizacionais. O SARS CoV 2 e a Covid 19 persistem presentemente sem previsões para a sua eliminação, com...

Publicação científica elaborada pelo grupo COVID-19 MD objetivando a disseminação do conhecimento sobre o exercício clínico em Medicina Dentária no contexto da COVID-19. Estas Normas de Orientação Clínica (NOC) são constituídas por três documentos: • NOC propriamente ditas. Agrupadas num documento que tem todas as questões elaboradas relativas ao e...

Aim To evaluate in a laboratory setting the effects of Endosequence BC Sealer HiFlow (Brasseler USA, Savannah, GA, USA), a novel calcium silicate‐based sealer developed for use in warm canal filling techniques, on human periodontal ligament stem cells (hPDLSCs). Methodology Eluates of EndoSequence BC Sealer HiFlow (BCHiF) (Brasseler USA), EndoSequ...

Update of scientific paper drawn up by the COVID-19 MD group aiming the dissemination of knowledge about the clinical practice in Dentistry in the context of COVID-19.

Infografia do Trabalho científico elaborado pelo grupo COVID-19 MD objetivando a disseminação do conhecimento sobre o exercício clínico em Medicina Dentária no contex da COVID-19

Pontos Chave do Trabalho científico elaborado pelo grupo COVID-19 MD objetivando a disseminação do conhecimento sobre o exercício clínico em Medicina Dentária no contex da COVID-19

Publisher: Copyright (c) 2019 Coimbra University PressISBN: 978-989-26-1986-6

Em Dezembro de 2019, o mundo assistiu à emergência de uma zoonose provocada por um novo tipo de Corona vírus o SARS CoV 2. Esta infeção rapidamente evoluiu para uma disseminação pandémica com consequências pesadas em todos os planos civilizacionais. O SARS CoV 2 e a Covid 19 persistem presentemente sem previsões para a sua eliminação, com grande in...

Em Dezembro de 2019, o mundo assistiu à emergência de uma zoonose provocada por um novo tipo de Corona vírus o SARS CoV 2. Esta infeção rapidamente evoluiu para uma disseminação pandémica com consequências pesadas em todos os planos civilizacionais. O SARS CoV 2 e a Covid 19 persistem presentemente sem previsões para a sua eliminação, com grande in...

Em Dezembro de 2019, o mundo assistiu à emergência de uma zoonose provocada por um novo tipo de Corona vírus o SARS CoV 2. Esta infeção rapidamente evoluiu para uma disseminação pandémica com consequências pesadas em todos os planos civilizacionais. O SARS CoV 2 e a Covid 19 persistem presentemente sem previsões para a sua eliminação, com grande in...

Introdução: A Rinosinusite Maxilar Crónica de origem dentária (um subtipo da Sinusite Odontogénica Maxilar) tem uma incidência significativa e muito variável entre os estudos (entre 10 a mais de 50%). Os dentes posterosuperiores encontram-se em estreita relação com os seios maxilares e em cerca de 40% dos casos as raízes dos molares estabelecem ínt...

Introdução: O objetivo do tratamento endodôntico é a prevenção ou a resolução da patologia pulpar e/ou periapical, com a recuperação da saúde dos tecidos periapicais. No en-tanto, existem situações em que a periodontite apical persis-te após o tratamento endodôntico convencional, sendo ne-cessária uma abordagem adicional como o retratamento não-cir...

ntrodução: O avanço do conhecimento da biologia e da fisiologia da inflamação pulpar têm vindo a possibilitar o alargamento das indicações da Terapia Vital Pulpar. Recentemente, a pulpotomia tem sido sugerida como tratamento alternativo à pulpectomia, em dentes permanentes maturos com sinais e sintomas compatíveis com o diagnóstico de pulpite irrev...

Apical periodontitis is a biofilm-mediated disease; therefore, an antimicrobial approach is essential to cure or prevent its development. In the quest for e�fficient strategies to achieve this objective, antimicrobial photodynamic therapy (aPDT) has emerged as an alternative to classical endodontic irrigation solutions and antibiotics. The aim of t...

Regenerative endodontic procedures (REPs) have emerged as a treatment option for immature necrotic teeth to allow the reestablishment of a newly formed vital tissue and enable continued root development. The apical papilla stem cells (SCAPs) play an important role in physiologic root development and may also contribute to further root development d...

apresentaram insucesso do tratamento endodôntico primário. Clinicamente, em ambos, foi feita a correção da cavidade de acesso, no sentido de permitir um acesso reto ao sistema de canais, pré alargamento do canal até à zona de bloqueio, uti-lização de limas de aço manuais com movimentos watch winding , sequencialmente, e utilizando medidas crescente...

This study evaluated the biocompatibility of a new silicone-based sealer (GuttaFlow Bioseal) in rat subcutaneous tissue and compared the results with those for GuttaFlow2 and AH Plus. Each of 16 Wistar rats received four subcutaneous tissue implants, namely, GuttaFlow Bioseal, GuttaFlow2, AH Plus, and one empty polyethylene tube. Eight rats were eu...

Aim: The purpose of the present study is to assess the color stability of two calcium silicate-based cements (CSCs) used in regenerative endodontic procedures (REPs). Methods: A total of 40 acrylic single-rooted transparent teeth, with immature apex, were used. Root canals were filled up to 3 mm below the level of the cementoenamel junction, wit...

Background: The development of materials with bioregenerative properties is critically important for vital pulp therapies and regenerative endodontic procedures. The aim of this study was to evaluate the cytocompatibility and cytotoxicity of a new endodontic biomaterial, PulpGuard, in comparison with two other biomaterials widely used in endodontic...

To evaluate the effect of immersion in 3% sodium hypochlorite solution in the resistance to cyclic fatigue of three nickel–titanium (NiTi) rotary file systems, ProTaper Next (PTN), Hyflex CM (CM), and Hyflex EDM (EDM), in a mechanical model featuring axial movement. Ninety instruments of three different NiTi rotary file systems, PTN (size 25, 0.06...

Root resorption (RR) refers to noninfectious damage related to the loss of hard and soft dental tissue that results from clastic cell activity. It is observed as a pathologic process that is predominantly asymptomatic in the permanent dentition and physiological during the shedding of primary teeth. Roots are protected by unmineralized organic ceme...

The purpose of the present study was to assess the proper time to perform a restoration (immediately or delayed) after placement of two calcium silicate-based cements (CSCs) and to test the performance of two different restorative protocols regarding shear bond strength (SBS). Seventy-five acrylic blocks were randomly divided into five groups (n =...

Objetivos: Este trabalho tem como objetivo avaliar a alteração da cor de dois cimentos à base de silicato de cálcio (Pro-Root ® MTA e BiodentineTM) após contato com sangue. Materiais e métodos: Foram utilizados 40 dentes artificiais iguais, de acrílico transparente, monorradiculares e com ápice imaturo, divididos em 4 grupos de acordo com os materi...

Introdução: Os odontomas são os tumores odontogénicos benignos mais frequentes. Clinicamente são considerados ha-martomas dos tecidos odontogénicos epitelial e mesenquima-toso. Segundo a OMS existem dois tipos: o odontoma complexo, em que os tecidos estão desorganizados e com um padrão amorfo, e o odontoma composto, no qual estão organizados asseme...

Objetivos: Este estudo tem como objetivo principal avaliar a biocompatibilidade de um novo cimento endodôntico à base de silicato de cálcio (Bioroot™RCS; Septodont, France) e de dois novos cimentos à base de silicone (Guttaflow® 2 e GuttaFlow® Bioseal; Coltène Whaledent, GmBH Co KG, Langenau, Switzerland). Neste projeto foi usado como referência um...

Aim To evaluate the biocompatibility of a new silicon-based sealer (GuttaFlow Bioseal) in subcutaneous tissues of rats in comparison with GuttaFlow2 and AH Plus. Methodology Twelve Wistar rats were selected. Each animal received 4 subcutaneous tissue implants: GuttaFlow Bioseal, GuttaFlow2, AH Plus and one empty Teflon tube (negative control group)...

Assess the antimicrobial efficacy and cytotoxic effects of 90s and 300s of 0.1 mg/mL Zn(II)e6Me versus 90s of Fotosan agent and 3% NaOCl at human root dentin blocks infected with 48h mixed biofilms of Enterococcus faecalis with Candida albicans (1:1).

Células estaminais são células indiferenciadas capazes de proliferação continuada e diferenciação numa grande variedade de tecidos quando expostas a determinados ambientes e estímulos. No adulto estas células podem ser isoladas de diferentes fontes, tais como a medula óssea, o sangue periférico ou o tecido adiposo. No entanto, as células estaminais...

Endodontic biofilms eradication from the infected root canal system remains as the primary focus in endodontic field. In this study, it was assessed the efficacy of antimicrobial Photodynamic Therapy (aPDT) with the Zn(II)chlorin e6 methyl ester (Zn(II)e 6 Me) activated by red light against monospecies and mixed biofilms of Enterococcus faecalis an...

Introduction: The aim of this study was to evaluate histologically the newly formed tissues after regenerative endodontic procedures (REPs) in dogs using either a blood clot (BC) or 2 different formulations of a chitosan hydrogel as scaffolds. Methods: Apical periodontitis was induced by inoculating immature teeth with oral plaque in 4 beagle do...

Introdução: A interrupção do desenvolvimento radicular representa um desafio clínico complexo. Objetivo: O objetivo principal deste estudo in vivo consiste em avaliar histologicamente o potencial regenerativo de diferentes matrizes à base de quitosano (ácido hialurónico:quitosano e pectina:quitosano) em comparação com o uso de coágulo sanguíneo em...

Objective: The aim of this study was the evaluation of cytotoxic effects of the new sealer GuttaFlow® 3 compared with GuttaFlow® 2 and AH Plus® on cell cultures. Methods: Root canal sealers were prepared according to the manufacturer and 0.1 mL of freshly mixed sealer was deposited at the bottom of a 96 well plate. After 30 minutes of initial setti...

Objetivo: Este trabalho tem como objetivo fazer uma avaliac¸ão comparativa da fadiga cíclicade 3 sistemas de limas diferentes utilizados em instrumentac¸ão mecanizada, o ProTaperNext Files®25/0.06 (Dentsply Maillefer,Ballaigues,Suíc¸a), o ProTaper®Universal F1 (DentsplyMaillefer, Ballaigues, Suíc¸a) e o HyFlexTM CM 25/0.06 (Coltène whaledent, Allst...

Histomorfometric analysis of the revascularization procedure – experimental study Aim The aim of this work is to determine the regenerative potential of immature teeth. Additionally, a novel regenerative strategy was characterized based on inducing a vital supporting structure, composed of blood clots and chitosan scaffolds, within the root canal...

Aim To review the literature on the efficacy of PDT efficacy to improve root canal disinfection protocols. Methodology A systematic literature review was undertaken to answer the PICO question ‘Does PDT improve the root canal asepsis revealing significant bacterial reduction in the root canal system?’. Articles of scientific relevance were searched...

O tratamento endodôntico não cirúrgico e a substituição de um dente por um implante são tratamentos dentários perfeitamente aplicáveis, porém a escolha da terapêutica deve ser a mais adequada a cada caso clínico e a decisão terapêutica deve incidir no sucesso a longo prazo e no custo-eficácia da mesma; sendo a decisão final do doente, devidamente i...

Introdução: O prognóstico do tratamento endodôntico depende da assepsia do sistema canalar, que promove a conservação ou restabelecimento da integridade dos tecidos periapicais. O protocolo da irrigação no sistema canalar é desafiante atendendo aos fatores que a adjudicam, como a resistência do biofilme, a insuficiente penetração da solução irrigad...