Joan DoidyUniversité de Poitiers | UP · EBI Ecologie & Biologie des Interactions (UMR 7267)
Joan Doidy
PhD
My works focus on sugar transport in pea crop (Pisum sativum) in response to plant-microbe interactions and drought.
About
24
Publications
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Introduction
My works focus on sugar transport and carbon metabolism in the agroecological pea crop (Pisum sativum) using physiological and molecular approaches combined to genomics and systems biology. We study carbon fluxes in responses to both biotic and abiotic environments, for instance plant-microbe interactions (arbuscular mycorrhiza) and resilience to drought stress.
Publications
Publications (24)
My poster, titled "Root functioning, carbon allocation and tolerance to water stress in Pea (Pisum sativum)," explores the differential responses of pea varieties to water deficit conditions. This study focuses on root development, carbon allocation strategies, and identifies phenotypic traits linked to high tolerance to water stress. By understand...
Agriculture is particularly impacted by global changes, drought being a main limiting factor of crop production. Here, we focus on pea ( Pisum sativum ), a model legume cultivated for its seed nutritional value. A water deficit (WD) was applied during its early reproductive phase, harvesting plant organs at two key developmental stages, either at t...
Pea (Pisum sativum) is an agroecological plant of the legume family cultivated for the high nutritional value of its
seeds. Its cultivation during periods of drought leads to modifications in source/sink relationships, resulting in
competition between the two sink organs, namely roots and seeds. These modifications can lead to the abortion
of flowe...
Grain legumes are major food crops cultivated worldwide for their seeds with high nutritional content. To answer the growing concern about food safety and protein autonomy, legume cultivation must increase in the coming years. In parallel, current agricultural practices are facing environmental challenges, including global temperature increase and...
Invertases are key enzymes for carbon metabolism, cleaving sucrose into energy‐rich and signaling metabolites, glucose and fructose. Invertases play pivotal roles in development and stress response, determining yield and quality of seed production. In this context, the repertoire of invertase gene families is critically scarce in legumes. Here, we...
In a greenhouse experiment, we investigated the influence of leek (Allium porrum) intercropping, mycorrhizal fungi and earthworms on the root-knot nematode infection by Meloidogyne incognita (RKN) and the growth and development of tomato plants (Solanum lycopersicum). Tomato plants were grown in pots, intercropped with leeks or in monoculture, and...
Sugar transporters play a crucial role for plant productivity, as they coordinate sugar fluxes from source leaf towards sink organs (seed, fruit, root) and regulate the supply of carbon resources towards the microorganisms of the rhizosphere (bacteria and fungi). Thus, sugar fluxes mediated by SUT (sucrose transporters), MST (monosaccharide transpo...
In plants, root is a typical sink organ that relies exclusively on the import of sugar from the aerial parts. Sucrose is delivered by the phloem to the most distant root tips and, en route to the tip, is used by the different root tissues for metabolism and storage. Besides, a certain portion of this carbon is exuded in the rhizosphere, supplied to...
Mycorrhizal colonization of plant roots increases the sink strength and creates an additional sugar demand for the plant. Nutrient uptake from soil and exchanges between fungal and plant partners forming mycorrhizal symbioses present a mean feature among the beneficial effects of these mutualistic relationships. In the mycorrhizal associations, in...
Soil nutrient acquisition and exchanges through symbiotic plant–fungus interactions in the rhizosphere are key features for the current agricultural and environmental challenges. Improved crop yield and plant mineral nutrition through a fungal symbiont has been widely described. In return, the host plant supplies carbon substrates to its fungal par...
Arbuscular mycorrhizal (AM) fungi are associated with about 80% of land plants. AM fungi provide inorganic nutrients to plants and in return up to 20% of the plant-fixed CO2 is transferred to the fungal symbionts. Since AM fungi are obligate biotrophs, unraveling how sugars are provided to the fungus partner is a key for understanding the functioni...
Background
Dynamic transcriptional regulation is critical for an organism’s response to environmental signals and yet remains elusive to capture. Such transcriptional regulation is mediated by master transcription factors (TF) that control large gene regulatory networks. Recently, we described a dynamic mode of TF regulation named “hit-and-run”. Th...
Understanding the mechanisms that underlie nutrient use efficiency and carbon allocation along with mycorrhizal interactions is critical for managing croplands and forests soundly. Indeed, nutrient availability, uptake and exchange in biotrophic interactions drive plant growth and modulate biomass allocation. These parameters are crucial for plant...
The Glomeromycota form a complex but extremely successful group of root symbionts that have accompanied land plants through evolution and survived across periods of important environmental change. They form a distinct, heterogeneous and unusual ensemble within the fungal kingdom which is characterized by obligate biotrophy, multinucleate nature, la...
We identified de novo sucrose transporter (SUT) genes involved in long-distance transport of sucrose from photosynthetic source leaves towards sink organs in the model leguminous species Medicago truncatula. The identification and functional analysis of sugar transporters provide key information on mechanisms that underlie carbon partitioning in pl...
In plants, long distance transport of sugars from photosynthetic source leaves to sink organs comprises different crucial steps depending on the species and organ types. Sucrose, the main carbohydrate for long distance transport is synthesized in the mesophyll and then loaded into the phloem. After long distance transport through the phloem vessels...
Sucrose and monosaccharide transporters mediate long distance transport of sugar from source to sink organs and constitute key components for carbon partitioning at the whole plant level and in interactions with fungi. Even if numerous families of plant sugar transporters are defined; efflux capacities, subcellular localization and association to m...
The Glomeromycota form a complex but extremely successful group of root symbionts that have accompanied land plants through evolution and survived across periods of important environmental change. They form a distinct, heterogeneous and unusual ensemble within the fungal kingdom which is characterized by obligate biotrophy, multinucleate nature, la...
Nitrogen (N) availability in the forest soil is extremely low and N economy has a special importance in woody plants that are able to cope with seasonal periods of growth and development over many years. Here we report on the analysis of amino acid pools and expression of key genes in the perennial species Populus trichocarpa during autumn senescen...
Questions
Question (1)
We have performed many cloning reactions (10 genes) using the pENTR/D-TOPO cloning kit, and it seems that many clones show an abberant insertion between the attL1 and the TOPO cloning site (please see pdf attachement). Here we show two independant cloning reactions, where it seems that our genes got inserted in front of an abberant sequences (in blue) that looks very similar to the 3' cloning sites that should normally be just upstream of the attl2. Also please note that we have two CACC sites, an intact CACC site of the 5' cloning site downstream of the attL1 (in red) and the CACC-ATG inserted afer the abberant blue sequence.
Please let me know if you have seen this problem before or if you can find an explanation to this issue. Lifetech offered a replacement kit, but did not investigate further for our cloning issue.
This happened with TOPO kit (lot number# 1917158).
