Jian Li

University of Prince Edward Island | UPEI · School of Business (1)

Recent conservation efforts to protect rare and endangered aquatic species have intensified. Nevertheless, the ornate spiny lobster (Panulirus ornatus), which is prevalent in the Indo-Pacific waters, has been largely ignored. In the absence of a detailed genomic reference, the conservation and population genetics of this crustacean are poorly under...

Exopalaemon carinicauda, a eurythermal and euryhaline shrimp, contributes one third of the total biomass production of polyculture ponds in eastern China and is considered as a potential ideal experimental animal for research on crustaceans. We conducted a high-quality chromosome-level genome assembly of E. carinicauda combining PacBio HiFi and Hi-...

Schistosome infection and schistosome‐derived products have been implicated in the prevention and alleviation of inflammatory bowel disease by manipulating the host immune response, whereas the role of gut microbiota in this protective effect remains poorly understood. In this study, we found that the intraperitoneal immunization with Schistosoma j...

Background Eukaryotic genes contain introns that are removed by the spliceosomal machinery during mRNA maturation. Introns impose a huge energetic burden on a cell; therefore, they must play an essential role in maintaining genome stability and/or regulating gene expression. Many genes (> 50%) in Plasmodium parasites contain predicted introns, incl...

Corona Virus Disease 2019 (COVID-19) is a highly prevalent and potent infectious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Until now, the world is still endeavoring to develop new ways to diagnose and treat COVID-19. At present, the clinical prevention and treatment of COVID-19 mainly targets the spike protein...

In this paper, a new time-dependent 2D/3D stochastic closed-loop geothermal system with multiplicative noise is developed and studied. This model considers heat transfer between the free flow in the pipe region and the porous media flow in the porous media region. Darcy’s law and stochastic Navier-Stokes equations are used to control the flows in t...

Nitrogenous waste is a global concern in aquatic ecosystems. In the shrimp farming system, feeding is the main input of nitrogen, which leads to the accumulation of nitrogenous waste, such as ammonia, nitrite, and nitrate. Nitrogen cycling is crucial for nitrogenous waste removal and for the stability of the aquaculture system. Under the action of...

Coronavirus disease 2019 (COVID-19) is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and characterized by dysregulated immune response. Studies have shown that the SARS-CoV-2 accessory protein ORF7b induces host cell apoptosis through the tumor necrosis factor alpha (TNF-α) pathway and blocks the production of int...

With the proposal and development of the internet of things (IoT), the ultra-high frequency (UHF) passive radio frequency identification (RFID) system based on backscatter communication technology has become one of the core technologies of the IoT due to its advantages of low power consumption and low cost. However, the insufficiency of the system...

This paper investigates the cluster synchronization of fractional‐order complex networks. Considering that impulsive control can reduce the update of controller, and the appearance of impulse is always dependent on each node in the networks instead of appearing at fixed instant, thus we design a variable‐time impulsive controller to control the con...

To evaluate the effects of Picochlorum on shrimp culture, Picochlorum was added to the culture system of Litopenaeus vannamei at concentrations of 1×10⁴cells/mL(KH_1), 2×10⁴cells/mL(KH_2) and 4×10⁴cells/mL(KH_4), and three culture systems with no Picochlorum addition were set up as control group(KH_D) . The water quality, diversity of microalgae co...

In this work, the advantage of Coulomb repulsion in the intermolecular forces experienced by molecules on the solid-liquid nanosized contact interface is taken, and the superior friction-reducing property of Cu 3 (PO 4 ) 2 ·3H 2 O (CuP) oil-based additives has been confirmed for titanium alloy. Three-dimensional (3D) CuP nanoflowers (CuP-Fs) with a...

Mutations in a Plasmodium de-ubiquitinase UBP1 have been linked to antimalarial drug resistance. However, the UBP1-mediated drug-resistant mechanism remains unknown. Through drug selection, genetic mapping, allelic exchange, and functional characterization, here we show that simultaneous mutations of two amino acids (I1560N and P2874T) in the Plasm...

The nucleocapsid protein of SARS-CoV-2 plays significant roles in viral assembly, immune evasion, and viral stability. Due to its immunogenicity, high expression levels during COVID-19, and conservation across viral strains, it represents an attractive target for antiviral treatment. In this study, we identified and characterized a single-stranded...

Marsupenaeus japonicus is an important marine crustacean species. However, a lack of genomic resources hinders the use of whole genome sequencing to explore their genetic basis and molecular mechanisms for genome-assisted breeding. Consequently, we determined the chromosome-level genome of M. japonicus. In total, 478.42 Gb of genomic sequencing dat...

In human aging, liver aging per se not only increases susceptibility to liver diseases but also increases vulnerability of other organs given its central role in regulating metabolism. Total liver function tends to be well maintained in the healthy elderly, so liver aging is generally difficult to identify early. In response to this critical challe...

Vitellogenin (Vtg) is a precursor of yolk proteins in egg-laying vertebrates and invertebrates and plays an important role in vitellogenesis and embryonic development. However, the Vtg family remains poorly characterized in Exopalaemon carinicauda, a major commercial mariculture species found along the coasts of the Yellow and Bohai Seas. In this s...

Macrocycles are key tools for molecular recognition and self-assembly. However, traditionally prevalent macrocyclic compounds exhibit specific cavities with diameters usually less than 1 nm, limiting their range of applications in supramolecular chemistry. The efficient synthesis of giant macrocycles remains a significant challenge because an incre...

This article describes the design of a dual-orthogonal $\pm$ 45 $^\circ$ polarized amplifying reconfigurable intelligent surface (RIS). To make possible the use of two orthogonal polarizations, an RIS architecture where single-port reflection amplifiers (RAs) are connected to the RIS antenna ports is considered. Instead of transistor-based RA de...

Background and objectives Nationwide data were used to explore factors associated with physical function in order to identify interventions that could improve and maintain physical function in the older people. Methods The physical function was assessed by gait speed (GS). We selected 2,677 male and 2,668 female older adults (aged ≥60) who could p...

Human aging is invariably accompanied by a decline in renal function, a process potentially exacerbated by uremic toxins originating from gut microbes. Based on a registered household Chinese Guangxi longevity cohort ( n = 151), we conducted comprehensive profiling of the gut microbiota and serum metabolome of individuals from 22 to 111 years of ag...

Severe COVID-19 patients exhibit impaired IFN-I response due to decreased IFN-β production, allowing persistent viral load and exacerbated inflammation. While the SARS-CoV-2 nucleocapsid (N) protein has been implicated in inhibiting innate immunity by interfering with IFN-β signaling, the specific underlying mechanism still needs further investigat...

Astaxanthin (Axn) is the primary pigment molecule in crustaceans associated with quality, health and growth traits, leading to increased marketing value. Axn can be contained within the protein complex crustacyanin (CRCN) to produce an array of different shell colours, or esterified with fatty acids (FA) for storage but also contributing additional...

Aging is a critical factor in spinal cord-associated disorders1, yet aging-specific mechanisms underlying this relationship remain poorly understood. To address this knowledge gap, we combined single-nucleus RNA sequencing with behavioral and neurophysiological analysis in non-human primates (NHPs). We identified motor neuron senescence and neuroin...

Cerebral malaria (CM) is a severe neurological complication of Plasmodium falciparum infection with acute brain lesions. Genetic variations in both host and parasite have been associated with susceptibility to CM, but the underlying molecular mechanism remains unclear. Here, we demonstrate that variants of human apolipoprotein E (hApoE) impact the...

Severe COVID-19 patients show impaired IFN-I response due to decreased IFN-β production, allowing persistent viral load and exacerbated inflammation. The SARS-CoV-2 nucleocapsid protein has been implicated in inhibiting IFN-I response through interfering with IFN-I signaling. This study reveals that SARS-CoV-2 nucleocapsid protein enhances interact...

Dopamine receptors (DARs) are important transmembrane receptors responsible for receiving extracellular signals in the DAR-mediated signaling pathway, and are involved in a variety of physiological functions. Herein, the D1 DAR gene from Marsupenaeus japonicus (MjDAD1) was identified and characterized. The protein encoded by MjDAD1 has the typical...

Antimalarial drug therapy is the recommended measure for malaria patients. In the chapter, the treatment for uncomplicated malaria, severe and cerebral malaria, and drug-resistant plasmodium parasites was described. Meanwhile, population prophylaxis for malaria is also reviewed.

Cardiac aging constitutes a significant risk factor for cardiovascular diseases prevalent among the elderly population. Urgent attention is required to prioritize preventive and management strategies for age-related cardiovascular conditions to safeguard the well-being of elderly individuals. In response to this critical challenge, the Aging Biomar...

Identification oncogenes is fundamental to revealing the molecular basis of cancer. Here, we found that FOXP2 is overexpressed in human prostate cancer cells and prostate tumors, but its expression is absent in normal prostate epithelial cells and low in benign prostatic hyperplasia. FOXP2 is a FOX transcription factor family member and tightly ass...

Aging of the vasculature, which is integral to the functioning of literally all human organs, serves as a fundamental physiological basis for age-related alterations as well as a shared etiological mechanism for various chronic diseases prevalent in the elderly population. China, home to the world’s largest aging population, faces an escalating cha...

Background Trichomoniasis caused by Trichomonas vaginalis, combined with its complications, has long frequently damaged millions of human health. Metronidazole (MTZ) is the first choice for therapy. Therefore, a better understanding of its trichomonacidal process to ultimately reveal the global mechanism of action is indispensable. To take a step t...

The internal lubrication of polyimide (PI) with added graphene oxides (GOs) and carbon nanotubes (MWCNTs), as well as their binary hybrid nano-additives (GCNTs), were contrastively investigated under dry sliding conditions. The friction coefficient of neat PI or its nanocomposites decreases as a stepwise increase of the load fitting a negative expo...

This paper develops a new 2D/3D stochastic closed-loop geothermal system with a random hydraulic conductivity tensor. We use the finite element method (FEM) and the Monte Carlo method (MCM) to discrete physical and probability spaces, respectively. This FEM-MCM method is effective. The stability for velocity and temperature is rigorously proved. Co...

Herein we report the design and synthesis of a terphen[n]arene derivative functionalised with sulfate acid ester groups. This water-soluble terphen[3]arene host effectively encapsulates a multitude of neuromuscular blocking agents (NMBAs) with high affinity, showing great potential as a NMBAs reversal agent in pharmaceutical research.

Nitrite is a common pollutant encountered in aquaculture systems. During intensive hatchery, accumulation of nitrite can cause massive mortality of juvenile crustaceans. However, the nitrite toxicity and cellular stress responses in juvenile crustaceans is not clearly understood. Here, we investigate the survival, energy metabolism, and cellular st...

Eyestalk ablation is an effective method to promote ovarian development in crustaceans. Herein, we performed transcriptome sequencing of ovary and hepatopancreas tissues after eyestalk ablation in Exopalaemon carinicauda to identify genes related to ovarian development. Our analyses led to the identification of 97,383 unigenes and 190,757 transcrip...

High alkalinity stress was considered as a major risk factor for aquatic animals surviving in saline-alkaline water. However, few information exists on the effects of alkalinity stress in crustacean species. As the dominant role of gills in osmotic and ionic regulation, the present study firstly evaluated the effect of alkalinity stress in Exopalae...

Integrated multitrophic aquaculture (IMTA) maximises the nitrogen cycle between system components, including bacteria. In order to maximise the bacterial role in nitrogen elimination in an IMTA system, we investigated the effect of bivalve culture on water quality and bacterial community structure in overlying water and sediment in the “shrimp-crab...

To study nitrogen removal by Ulva lactuca and its effects on the structure of the bacterial community and abundance of nitrogen cycle functional genes in the ‘sea cucumber–shrimp–crab–fish’ integrated multi-trophic aquaculture (IMTA) system, we analysed bacterial community composition by Illumina MiSeq high-throughput sequencing and nitrogen cyclin...

Florfenicol (FLR), a broad-spectrum antibacterial agent, is commonly used in the breeding and cultivation of aquatic animals. microRNAs (miRNAs) control gene expression by mediating post-transcriptional mRNA repression. However, not much has been reported regarding their functions when crustaceans encounter FLR stress. Herein we observed histologic...

Cerebral malaria (CM), caused by Plasmodium falciparum, is the primary cause of death from severe malaria. Even after immediate parenteral therapy with antimalarial drugs, the mortality rate remains 15 to 25%. Currently, no effective therapeutic agents are available for the radical treatment of CM. Thus, further in-depth explorations of adjuvant th...

The dinoflagellate Prorocentrum minimum is known to affect the normal physiological function of Exopalaemon carinicauda by inducing oxidative stress, apoptosis, and cellular injury. To study the effects of P. minimum on the immune defense system of shrimp, E. carinicauda were exposed to 5 × 103 cells mL−1 and 5 × 104 cells mL−1 of P. minimum for 33...

Ammonia is a significant concern during hatchery culture in brachyuran species, and its accumulation may lead to abortive moulting and large-scale deaths of the early juveniles. To date, the underlying mechanism for ammonia-induced alteration of the moulting process is still unknown. In this study, we aimed to investigate the effects of ammonia on...

The effects of different solar radiation intensities, heat flow density of indoor heat sources, outdoor wind speed, and the relative location of indoor heat sources on the natural ventilation performance of solar chimneys are investigated through three-dimensional numerical simulations. The mechanism of the mutual coupling of the solar chimney effe...

In this work, the advantage of Coulomb repulsion in the intermolecular forces experienced by molecules on the solid-liquid nanosized contact interface is taken, and the superior friction-reducing property of Cu3(PO4)2·3H2O (CuP) oil-based additives has been confirmed for titanium alloy. 3D CuP nanoflowers (CuP-Fs) with a strong capillary absorption...

The economic and ecological effects of three shrimp farming models commonly adopted in China, including the indoor super-intensive culture model (ISIC), the pond integrated multi-trophic aquaculture model (PIMTA), and the pond mono-culture model (PMC) were evaluated in this study. The economic and ecological effects of the three models were quantit...

Friction and wear are ubiquitous, from nano-electro-mechanical systems in biomedicine to large-scale integrated electric propulsion in aircraft carriers. Applications of nanomaterials as lubricating oil additives have achieved great advances, which are of great significance to control friction and wear. This review focuses on the applications of na...

Temperature sensing based on emerging techniques such as the novel electromagnetic resonant-unit has been the vital research direction. However, current works only focused on the initial and principal verifications, and it is far away to the real application. In this paper, a temperature sensor based on mercury-inspired electromagnetic resonant-uni...

A compacted body was fabricated by pulverulent polyimide (PI) block copolymers using solid-like state compression molding (SCM) technique. Polymer heated to solid-like state, i.e. the high-elastic non-melting state above the glass transition temperature ( T g ) and well below melting temperature, could achieve plasticity due to dramatic decreases i...

In this work, the superhydrophobic surfaces with micro-nano composite structure was successfully prepared by one-step electrodeposition based on Ca-myristic acid complex onto Cu substrate. The performance of delaying frost growth on micro-nanostructure superhydrophobic copper surfaces was explored, and the application of superhydrophobic materials...

This study aimed to determine the immunity, survival rate, and disease resistance of Litopenaeus vannamei treated using quercetin and florfenicol alone or in combination, after infection with acute hepatopancreatic necrosis syndrome caused by Vibrio parahaemolyticus (VPAHPND). After infection with VPAHPND, different types of feed were given to the...

Dual-pressure evaporation organic Rankine cycle shows a good outlook in the power generation of medium-to-low temperature heat. Adding internal heat exchanger (IHE) is an important potential way to enhance its performance. While, the impacts of adding IHE have not been analyzed especially. The effectiveness, quantitative enhancement effects, and su...

Geometric variables and surface chemistry of nanomaterials as lubricant additives both affect the details on the interacting frictional surface. Herein, copper phosphate nanosheets (CPNs) were synthesized by a simple and facile method, which exhibited extraordinary tribological properties as the novel lubricating oil nanoadditives. Compared with th...

As a euryhaline shrimp, the ridgetail white prawn Exopalaemon carinicauda is strongly adaptable to salinity. Exploring the effect of long-term low salinity stress on ovarian development in E. carinicauda is essential to promote its culture in a non-marine environment. In this study, we performed biochemical assays and ovary histology analysis, find...

Since single nucleotide polymorphisms (SNPs) have attracted attention, there have been many explorations and improvements in screening and detection methods for SNPs. Traditional methods are complex and time-consuming and rely on expensive instruments. Therefore, there is an urgent need for a low-cost, simple, and accurate method that is convenient...

Saline–alkaline water limits the growth and survival of aquatic animals due to its high carbonate alkalinity, high pH, and various ion imbalances. The ridgetail white prawn Exopalaemon carinicauda is strongly adaptable to the saline–alkaline water, making it an excellent candidate species for large-scale aquaculture in saline–alkaline areas. To exp...

Tannic acid (TA) has great potential as a new feed additive. In this study, we investigated the effects of dietary TA on growth, digestion, nonspecific immunity, and resistance to ammonia stress and intestinal microbiota in Litopenaeus vannamei. The shrimp were fed diets containing different levels of TA: 0 mg/kg (TA-0), 200 mg/kg (TA-200), 400 mg/...

Viruses struggle to reproduce after infecting cells, and the host eliminates infected cells through apoptosis to prevent virus spread. Cells adopt a special ubiquitination code to protect against viral infection, while ORF7a manipulates and exploits the ubiquitin system to eliminate host cells' effect on apoptosis and redirect cellular pathways in...

Ultrahigh molecular weight polyethylene (UHMWPE) has been extensively used in various tribological systems because of its outstanding tribological properties and excellent overall performance. Compression molding is the main molding method for UHMWPE, and the process parameters of molding have a profound effect on its material properties. In this s...

In this work, blended composites with ultra‐high molecular weight polyethylene (UHMWPE) as matrix polymer, α‐zirconium phosphate (α‐ZrP) as filler, and sodium polyacrylate (PAANa) as compatibilizer were prepared. The interfacial interaction between PAANa as a compatibilizer and the components of α‐ZrP/UHMWPE was studied by molecular dynamics simula...

Ultra-high molecular weight polyethylene (UHMWPE) is prone to deformation and wear under harsh conditions of dry friction with high contact stress, and failing friction parts. In this work, CZrP/UHMWPE composites with different organic-modified α-zirconium phosphate (CZrP) contents were prepared. The tribological properties of the GCr15/composites...

High pH is one of the main stressors affecting the shrimp survival, growth, and physiology in aquaculture ponds, but the cellular and molecular mechanism responsible for high pH stress has not been elucidated in shrimp. In this study, the shrimp acid-base disturbance and gill cell alterations were significantly observed and then single cell RNA-seq...

In this paper, a two-grid scheme for a time-dependent closed-loop geothermal system is proposed. The scheme solves a coupled partial differential equation model for this system on a coarse mesh with a mesh size of H and a decoupled problem on a fine mesh with a mesh size of h. In theoretical analysis, the stability and convergence of the two-grid s...

The Penaeus vannamei is an important shrimp species with enormous commercial and ecological values. In production process, the air exposure resistance is vital for live transportation without water. We tested the air exposure resistant ability of P. vannamei, and carried out gill histological observation and gene expression analysis. The physiology...

The ridgetail white prawn, Exopalaemon carinicauda is an important cultured seawater species in China. As female E.carinicauda grows faster than males, it is significant to search for the differentially expressed genes (DEGs) between males and females. However, there is no public available E.carinicauda genome data, and genes related to E. carinica...

Astaxanthin (Axn), a common aquatic feed additive, can enhance immunity, improve the antioxidant capacity of the crustacean and then improve the anti-stress ability of crustaceans. Exopalaemon carinicauda (E. carinicauda) is an economically important fishery species in China that has been found that dietary Axn can significantly increase ACP and AK...

Suspension-feeding bivalves are the key driver unit for cycling of material and energy in integrated multi-trophic aquaculture (IMTA) system, but only few studies have examined their bioturbation effects in land-based aquaculture system. In the present study we explored the effects of different species (Ruditapes philippinarum, Sinonovacula constri...

Vibrio parahaemolyticus belongs to an expanding group of aquatic pathogens that are widely distributed in aquatic environments. This species is a lethal pathogen for a number of economically important marine crabs. However, studies exploring host–vibrio interactions between V. parahaemolyticus and crabs are scarce, and therefore, the underlying mol...

We report here an isocyanide‐based multicomponent reaction to assemble a number of tricyclic chromenopyrrole derivatives with diastereoselectivity. Mechanistically, this protocol employs phenol as electron‐rich species, thus following bicyclization process to approach a series of diverse structures. image