Jean Rene Grezes

Laboratoire Prive · R & D of DYES & FLUOROCHROMES

I synthesized a lot of mono-, di- , tri- tetra-substitued cyano-acridines 1970 for DNA and Cancer Research .Among them, 3-aminoacridine-9-carbonitrile and 3,6-diaminoacridine-9-carbonitrile (so-called 9-CNPF (my lab)or CTX-1 (David Wald lab) and 3,6-diamino-2-methylacridine-9-carbonitrile and 3,6-diamino-2,7-dimethylacridine-9-carbonitrile and 3,6-...

https://www.researchgate.net/publication/294920221_Identification_of_a_Small_Molecule_That_Overcomes_HdmX-Mediated_Suppression_of_p53?enrichId=rgreq-335e914be70d9931727769f6ea72c82a-XXX&enrichSource=Y292ZXJQYWdlOzI5NDkyMDIyMTtBUzo1Nzg3MjYxMTk1OTE5MzZAMTUxNDk5MDQ2NzAyNQ%3D%3D&el=1_x_3&_esc=publicationCoverPdf The same dye 9-CN-PF (my lab) called CT...

Investigations into the detection and identification of nucleic acids, organelles in living and fixed cells are-largely based on the use of molecular fluorescent probes. Fluorescence spectroscopy and microspectrof1uorometry are very sensitive techniques capable of giving informations on various aspects of the structure and dynamics of biomacromolec...

This poster is the continuation of the study of J. Bartos and P. Wirtz (Uni Freiburg/Germany) Published in Z. Tierpsychol. 65, 103-107 (1984) (see the colour plate /Next side)

The benzimidazole dye H8208 (2-(4-aminophenyl)-5-(4-methylpiperazin-1-yl) benzimidazole) has been shown to intercalate into calf thymus DNA and into polyinosinic-polycytidylic acid (a model for A conformation DNA) by a variety of spectroscopic techniques. The binding affinity of the dye was found to be similar to both nucleic acids.

Spectrophotometry, thermal denaturation, sedimentation, and viscometric techniques were used in a study of interaction of double helical DNA with an asymmetric phenazinium derivative, aposafranine. The results obtained indicate that aposafranine binds to DNA by a single binding mode, a wedge-like partial intercalation.

The Romanowsky-Giemsa staining (RG staining) has been studied by means of microspectrophotometry using various staining conditions. As cell material we employed in our model experiments mouse fibroblasts, LM cells. They show a distinct Romanowsky-Giemsa staining pattern. The RG staining was performed with the chemical pure dye stuffs azure B and eo...

The Romanowsky-Giemsa staining (RG staining) has been studied by means of microspectrophotometry using various staining conditions. As cell material we employed in our model experiments mouse fibroblasts, LM cells. They show a distinct Romanowsky-Giemsa staining pattern. The RG staining was performed with the chemical pure dye stuffs azure B and eo...

David Wald and Mukesh Agarwal wrote in the publication about CTX1 activity: Materials and Methods: CTX1 was obtained from the Development Therapeutics Program at the National Cancer Institute. In General Information on the NCI Screening Program, one can read: " the testing is performed free of charge and you, as the supplier, retain all proprietary...

Es wurden analysenreine Proben der Romanowsky-Farbstoffe Eosin Y, Erythrosin B und Tetrachlorfluoreszein hergestellt.Im DC der Farbstoffproben konnten keine Verunreinigungen nachgewiesen werden. Die Absorptionsspektren der Farbstoffdianionen in wriger alkalischer Lsung und der Farbstoffsuren in 95%igem Ethanol wurden bei sehr kleinen Farbstoffkonze...

Page 2 shows the structure of the new dyes cited in the abstract of the proceedings of the congress . The proof of the structure was done per NMR, UV-Vis, MS-Maldi-TOF, Cyclovoltamogrammes spectra and is avaible upon request.

Analytically pure samples of the Romanowsky dyes eosin y, erythrosin b and tetrachlorofluorescein are prepared. DC of the dye samples shows no contaminations. We measured the absorption spectra of the dye dianions in alkaline aqueous solution and of the dye acids in 95% ethanol at very low dye concentrations. The molar extinction coefficients of th...

The possibility of a specific nuclear staining with 9-cyano-3,6-diamino-acridine (9CNPF) and 9-cyano-2,7-dimethyl-3,6-diamino-acridine (9CNDMPF) according to the Feulgen staining was shown several years ago. The base of 9CNPF and 9CNDMPF in solution (water, ethanol,…) is yellow and the cation of them is red The measurement of nucleus per absorption...

Azure B is the most important Romanowsky dye. In combination with eosin Y it produces the well known Romanowsky-Giemsa staining pattern on the cell. Usually commercial azure B is strongly contaminated. We prepared a sample of azure B-BF4 which was analytically pure and had no coloured impurities. The substance was used to redetermine the molar exti...

Azure B is the most important Romanowsky dye. In combination with eosin Y it produces the well known Romanowsky-Giemsa staining pattern on the cell. Usually commercial azure B is strongly contaminated. We prepared a sample of azure B-BF4 which was analytically pure and had no coloured impurities. The substance was used to redetermine the molar exti...

Die Bindung des Farbstoffs Tetramethylethidiumbromid TME (2,7-Bis(dimethylamino)-9-phenyl-10-äthyl-phenanthridinium-bromid) an Kalbsthymus-DNA wurde absorptionsspektroskopisch untersucht. Der Farbstoff kann durch Na⊕- und Mg2⊕-Ionen aus seiner Bindung an DNA vollständig verdrängt werden. Er geht nur eine kompetitive Bindung ein (Ks2 = 1,6 · 105 m−1...