Javad Harati

shenzhen institute of advanced technology

Topographical cues on material surfaces are crucial for guiding the behavior of nerve cells and facilitating the repair of peripheral nerve defects. Previously, micron-grooved surfaces have shown great potential in controlling nerve cell alignment for studying the behavior and functions of those cells and peripheral nerve regeneration. However, the...

Direct neuronal reprogramming of somatic cells into induced neurons (iNs) has been recently established as a promising approach to generating neuron cells. Previous studies have reported that the biophysical cues of the in vitro microenvironment are potent modulators in the cell fate decision; thus, the present study explores the effects of a custo...

Polydopamine (PDA) is a biocompatible cell-adhesive polymer with versatile applications in biomedical devices. Previous studies have shown that PDA coating could improve cell adhesion and differentiation of human mesenchymal stem cells (hMSCs). However, there is still a knowledge gap in the effect of PDA-mediated protein adsorption on the epigeneti...

The prime editor is a versatile tool for targeted precise editing to generate point mutations, small insertions or small deletions in eukaryotes. However, canonical PE3 system is less efficient, notably in primary cells or pluripotent stem cells. Here we employed RNA polymerase II promoter instead of RNA polymerase III promoter, whose application i...

The generation of blood cells in a significant amount for clinical uses is still challenging. Human pluripotent stem cells-derived hemopoietic cells (hPSC-HCs) are a promising cell source to generate blood cells. Previously, it has been shown that the attached substrates are crucial in the maintenance or differentiation of hPSCs. In this study, a n...

Hybrid surfaces with tunable topography, chemistry, and stiffness have potential to rebuild native extracellular matrix (ECM) and manipulate cell behavior in vitro. However, the fabrication of controllable hybrid surfaces is still challenging. In this study, colloidal self-assembly technology was used to program particles into highly ordered struct...

Abstract Induced pluripotent stem cells (iPSCs) can self-renew indefinitely in culture and differentiate into all specialized cell types including gametes. iPSCs do not exist naturally and are instead generated (“induced” or “reprogrammed”) in culture from somatic cells through ectopic co-expression of defined pluripotency factors. Since they can b...

Abstract: rnA total of 78 full-length protein sequences of α-amylase from different source organisms were subjected to phylogenetic analysis, multiple sequence alignment (MSA), motif search, and physiochemical properties. The phylogenetic tree was built using the Maximum Likelihood (ML) method in Molecular Evolutionary Genetics Analysis (MEGA) soft...

A total of 78 full-length protein sequences of α-amylase from different source organisms were subjected to phylogenetic analysis, multiple sequence alignment (MSA), motif search and physiochemical properties. The phylogenetic tree was built using the maximum likelihood method in molecular evolutionary genetics analysis (MEGA) software and was point...

The objective of the current study was to enzymatically characterize immobilized phytase as a glycoprotein on an optimal blend of starch/PAAm nanofibers. Aspergillus niger phytase (phyA) was first expressed in Hansenula polymorpha using formate dehydrogenase promoter and α-mating factor signal peptide. Response surface methodology was then used to...

In this work, poly (acrylic acid) (PAA) and PAA/multi-walled carbon nanotubes (MWNTs) nanofibrous membranes are fabricated by electrospinning to immobilize acetylcholinesterase (AChE). 3-Aminopropyltriethoxysilane (APTES) and glutaraldehyde are used for surface modification and PAA membrane stabilization in aqueous media. The structure of the nanof...

In this work, activity of immobilized phytase on blended polyacrylamide (PAAm)/starch nanofibers produced via electrospinning was optimized by computer-aided statistical method of experimental design, response surface methodology (RSM). Thereafter, the role of polymer concentration and percentage of starch in period of enzyme activity were optimize...

Phytic acid is the dominant form of phosphorous in plant seeds. However, phytic acid cannot be utilized by animals and causes them serious phosphate deficiency. Utilization of phytase as an animal feed additive can affect liberation of phosphor and its mineral availability. In this study, heterologous expression of the A. niger phyA gene was invest...

Introduction: Phytase is able to change phytic acid into the myo-inositiol and inorganic phosphate, so it has been used as a cereal food additive in monogastric animal's food. The aim of this study was to eamine cloning, expression and culture optimization of gene encoding Aspergillus niger NRRL3135 phytase in Hansenula polymorpha host. Materials a...