Jakub Sawicki

University of Warmia and Mazury in Olsztyn · Department of Botany and Evolutionary Ecology

1. Anthoceros punctatus L. Contributors: B. Cykowska and F. Mogro Bolivia: Dept. Tarija, Prov. Arce, near Reserva de Fauna Tariquia reserve, next to the road from Sidras to Camberi, 22u12909.70S, 64u31937.50W, 989 m a.s.l., on wet clay soil of roadside slope, 18 November 2010, leg. B. Cykowska 10255 & F. Mogro (KRAM). Anthoceros punctatus L. is one...

Orthotrichum pamiricum Plášek & Sawicki is described as a new species from the western foothills of the Pamir Mountains of the Tajik–Afghan border in Central Asia. It is placed in Orthotrichum Hedw. subg. Pulchella (Schimp.) Vitt. The species is characterized by obtuse leaf apices, cryptoporous stomata, an exostome composed of 8 pairs of the teeth...

This paper is both a review and a study. It discusses the taxonomic status of Yellow Archangel (Galeobdolon luteum Huds.) from historical and contemporary perspectives, and gives a comprehensive list of synonyms for the discussed genera, species and lower taxonomic units, including their publication details. In the study it is postulated that G. lu...

The aim of this work was to evaluate the suitability of selected DNA regions in the barcoding of plants, based on the species belonging to the genus Lamium (Lamiaceae). For this purpose, nine chloroplast barcodes, i.e. accD, matK, rbcL, rpoA, rpoB, rpoC1, rpoC2, trnH-psbA, trnL-trnF, as well as ITS nuclear region, and intron of mitochondrial nad5 g...

RNA polymerase type I (plastid-encoded polymerase, PEP) is one of the key chloroplast enzymes. However, the rpo genes that encode its subunits (rpoA, rpoB, rpoC1 and rpoC2) are relatively rapidly evolving sequences. The aim of this study was to investigate the rate of the molecular evolution of rpo genes and to evaluate them as phylogenetic markers...

Dioecy is generally associated with sexual dimorphism, which can be noticed with the naked eye, but in plants the sex of an individual, at the early stages of development before flowering, is difficult to diagnose. The situation is more complicated in bryophytes, where determination of sex in adult individuals can be problematic or even impossible....

Here, we report the results of next-generation sequencing on the GS Junior system to identify a large number of microsatellites from the epiphytic moss Orthotrichum speciosum. Using a combination of a total (non-enrichment) genomic library and small-scale 454 pyrosequencing, we determined 5382 contigs whose length ranged from 103 to 5445 bp. In thi...

We identify a large number of microsatellites from Galium trfidum, a plant species considered rare and endangered in Central and Western Europe. Using a combination of a total enriched genomic library and small-scale 454 pyrosequencing, we determined 9755 contigs with a length of 100 to 6192 bp. Within this dataset, we identified 153 SSR motifs in...

Relics of Malva alcea are found in Central and Western Europe. A similar taxon, M. excisa, is native to the eastern parts of Europe. According to selected sources, the geographical range boundary of the above taxa intersects Poland. Taxonomic research relying on key morphological features (the depth of corolla petal incisions and the type of hairs...

Ellis L.T., Bednarek-Ochyra H., Ochyra R., Cykowska B., Dulin M.V., Ezer T., Kara R., Flores J.R., Suárez G.M., Garcia C., Martins A., Sérgio C., Garilleti R., Kirmaci M., Agcagil E., Kurbatova L.E., Lebouvier M., Papp B., Szurdoki E., Philippov D.A., Plášek V., Pócs T., Sabovljević M., Sawicki J., Sim-Sim M., Szücs P., Bidló A., Váňa J., Vigalondo...

Orthotrichum affine var. bohemicum Plášek & Sawicki was described as a new variety from the Czech Republic in 2011. However, in the original diagnosis the holotype specimen and the type location were not conclusively designated. To validate this new name, the current article clearly states which specimen is selected as the holotype and the type loc...

Pulsatilla patens is a rare and endangered plant species in many areas of Europe and protected under the Bern Convention and it is listed in Annex II and Annex IV to the Habitats Directive. In this study we developed 12 novel microsatellite loci using via 454 sequencing. We determined 11,220 contigs with a length of 156-11,384 bp. Within this datas...

33 contributors report on 21 bryophyte species, collected in Europe, SW Asia, Africa, South America, on islands in Subantarctic and in South Indian Ocean. There are interesting records from Turkey (Crossidium aberrans, Zygodon catarinoi), Portugal (Schistidium strictum), Serbia (Ditrichum lineare), Ukraine (Dichelyma capillaceum), and from other co...

Calypogeia sphagnicola is one of nine species of the genus Calypogeia known in Europe. Occurrence of the species is closely connected with peat bogs. Nowadays, two forms of this species are distinguished—C. sphagnicola f. sphagnicola and C. sphagnicola f. paludosa. The results of the present study, based on two classes of markers—isozymes and seque...

Abstract Genetic relationships between 27 Orthotrichum species of the subgenera Orthotrichum and Pulchella were reconstructed using the internal transcribed spacer regions 1 and 2, the chloroplast trnH‐psbA region, and intron–exon splice junction (ISJ) and inter‐simple sequence repeats (ISSR) markers. A phylogenetic analysis did not reflect the cur...

Genetic studies of Calypogeia sphagnicola (Arnell & J. Perss.) Warnst. & Loeske revealed that besides C. sphagnicola f. sphagnicola and C. sphagnicola f. paludosa (Warnst.) Schust., recently recognized as separate species, a third genetically distinct group of plants connected with Sphagnum communities can be distinguished in the Calypogeia genus i...

Mechanismus of sex determination in plants. – Čas. Slez. Muz. Opava (A): 61: 123-129, 2012. Ab s tr a ct: Mechanisms of sex determination in plants are popular research topic. Research studies are initiated to describe sex determination mechanisms and develop molecular methods for sex differentiation in plant species where sexual dimorphism is weak...

Orthotrichum affine var. bohemicum Plášek & Sawicki, var. nova, is a confirmed record of a new variety from the Czech Republic. The new taxon is described and illustrated by photos and drawing. It is characterized by having superficial stomata and a peristome with 16 endostome segments (practically as long as exostome teeth). Molecular data indicat...

RAPDs, ISJs, ISSRs, ITS and katGs were applied to determine genetic relationships between common Sphagnum species of the section Acutifolia. Twenty populations were genotyped using ten ISJ primers, 12 pairs of katG primers, 10 ISSR and 10 RAPD primers, and a restriction analysis of ITS1 and ITS2. ISSR and katG markers revealed the greatest number o...

The reasons for low intrapopulation genetic variation in Lamium incisum Willd The paper presents results of a study which aim was preliminary screening of intrapopulation genetic variability in Lamium incisum Willd. This weed species is rarely distributed in Poland and lessening its count during the last years. As a plant inhabiting anthropogenic s...

Two morphologically distinct groups of the Calypogeia fissa complex were found in Europe Two genetically distinct groups (P S and P B ) detected previously within the C. fissa complex in Europe were studied with respect to 47 morphometric characters. The two examined groups differed statistically significantly with respect to 34 morphological trait...

Iran: Gilan province, Masouleh to Khalkhal road, 48u499E, 37u159N, 1400 m, on moist soil, 2 June 1996, leg. S. Shirzadian 0297 (IRAN). This species is found in very wet and damp grasslands and is distributed in Europe, America and Asia (Smith, 2004). Many bryologists (e.g. Lawton, 1971; Horton & Vitt, 1976) have elaborately discussed the distinctiv...

Molecular identification of sex in dioecious moss Nyholmiella obtusifolia (Orthotrichaceae) on the basis of ISSR markers. – Čas. Slez. Muz. Opava (A), 60: 1-6, 2011. Abstract: As far as an unisexuality in the animal kingdom is typical, in higher plants there most species are bisexual. Whereas in haploid bryophyte it can be noticed that dioecy in th...

Recent data on occurrence the sporophytes in populations of dioecious moss, Nyholmiella obtusifolia in North-Eastern Poland Moss Nyholmiella obtusifolia is relatively common in Poland, but mostly sterile specimens are found in herbarium collections. Sporogenes were created very rare. During bryological explorations conducted in NE Poland in 2009 -...

The distribution of Bazzania trilobata in Poland coincides with two parts of the natural distribution range of Norway spruce: the mountains of southern Poland and the northeastern lowlands. The occurrence of this species is connected with primeval forest communities and it was recognized as a relict of them. Genetic variation of 10 populations of B...

Abstract Two Orthotrichum species of the subgenus Orthophyllum were compared with other representatives of this genus using internally transcribed spacer regions 1 and 2, the chloroplast trnH–psbA region, and inter-simple sequence repeat (ISSR) and intron–exon splice conjunction (ISJ) markers. The ISSR and ISJ markers used revealed many bands and m...

Genetic relationships between some of Malva species as determined with ISSR and ISJ markers Two categories of DNA markers were used to determine genetic relationships among eight Malva taxa. A maximum parsimony analysis validated the division of the genus Malva into the sections Bismalva and Malva. The species classified into those sections formed...

The location of stomata and the structural details of exostome teeth and endostome segments are among the most important taxonomic features used for identifying Orthotrichum species. Certain species pairs (e.g. O. stramineum vs. O. pallens; O. pumilum vs. O. stellatum) are distinguished based on the presence or absence of hair on the vaginula. The...

The study presents a phylogenetic analysis of species of the moss genus Orthotrichum. ITS1 and ITS2 for 30 species were sequenced. The results do not fully reflect the cur-rent division of Orthotrichum into subgenera and sections. Molecular data divide the genus into two groups of species with superficial and immersed stomata, and indicate a clear...

Eight Orthotrichum species of subgenus Gymnoporus were compared using the internally transcribed spacer regions-1 and -2 and the chloroplast trnH-psbA region. A phylogenetic analysis did not reflect the current division of this subgenus into sections Affinia and Leiocarpa. The investigated sequences revealed a close relationship between O. striatum...

Orthotrichum moravicum Plášek & Sawicki, sp. nov., is a confirmed record of a new species from the Czech Republic. The new taxon is described in subgen. Pulchella and illustrated by photos. Molecular data indicating the phylogenetic placement of the species are presented, indicating a sister relationship with O. pallens.

Chamaedaphne calyculata is rare and endangerded species of Polish flora. The genetic variation within and among ten polish population of leatherleaf was analysed by ISSR and ISJ markers. The analysis revealed a total of 160 loci with an average of 13.3 bands per primer. We expected a low level of genetic diversity of this narrowly distributed speci...

The liverwort Aneura pinguis is represented in Europe by 4 cryptic species (A, B, C and D). They differ in morphology and ecological preferences. Three molecular marker systems (RAPD, ISJ and katG) were used to identify particular cryptic species. Ten samples of 4 cryptic species of A. pinguis and 2 samples of A. maxima (as an outgroup) were analys...

We present a protocol for use of intron-exon splice junction (ISJ) markers in studies on bryophytes. The advantages and disadvantages of the method are discussed and compared with other PCR-based markers like AFLP, ISSR and RAPD.

Comparison of three Polygonatum species from Poland based on DNA markers. — Ann. Bot. Fennici 43: 379–388. Four categories of DNA markers were used to determine genetic similarity of three spe-cies of Polygonatum, i.e. P. multiflorum, P. odoratum and P. verticillatum, occurring in Poland. Four populations per species, represented by ten plants per...