Indra Singh

Banaras Hindu University | BHU · School of Biotechnology

African swine fever (ASF) has emerged as a threat to swine production worldwide. Evasion of host immunity by ASF virus (ASFV) is well understood. However, the role of ASFV in triggering oncogenesis is still unclear. In the present study, ASFV-infected kidney tissue samples were subjected to Illumina-based transcriptome analysis. A total of 2463 upr...

Investigating the role of host genetic factors in COVID-19 severity and susceptibility can inform our understanding of the underlying biological mechanisms that influence adverse outcomes and drug development. Here we present a second updated genome-wide association study (GWAS) on COVID-19 severity and infection susceptibility to SARS-CoV-2 from t...

African swine fever (ASF) is a continual economical threat to the global piggery sector. The host immune evasion caused by African swine fever virus (ASFV) is well understood. However, the ASF virus's significance in oncogenesis is uncertain. In the present study, ASFV infected kidney tissue samples were subjected for Illumina based transcriptome a...

The present study aimed to generate antibodies against predicted B cell epitopic peptides encoding bAMH for developing different ELISA models. Sandwich ELISA was determined to be an excellent technique for assessing bAMH in bovine plasma based on sensitivity tests. The assay's specificity, sensitivity, inter- and intra-assay CV, recovery %, Lower l...

Porcine reproductive and respiratory syndrome (PRRS) is an important economical disease in the global swine industry. The accurate detection of the PRRS virus (PRRSV) antigen is essential for the disease control and prevention programme. In this study, an indirect enzyme-linked immunosorbent test (PRRSVCD163-iELISA) was developed for the detection...

The goal of this study was to compare the global gene expression profile in cardiac tissues of pig infected with porcine circovirus 2 (PCV2) to that of healthy cells. Since PCV2 infection causes severe cardiovascular lesions, the myocardial tissue model was chosen for this study. In High-throughput transcriptome analysis, DESeq2 and CLC genomics wo...

Different human races across the globe responded in a different way to the SARS-CoV-2 infection leading to different disease severity. Therefore, it is anticipated that host genetic factors have a straight association with the COVID-19. We identified a total 6, 7, and 6 genomic loci for deceased-recovered, asymptomatic-recovered, and deceased-asymp...

In India, during the second wave of the COVID-19 pandemic, the breakthrough infections were mainly caused by the SARS-COV-2 delta variant (B.1.617.2). It was reported that, among majority of the infections due to the delta variant, only 9.8% percent cases required hospitalization, whereas only 0.4% fatality was observed. Sudden dropdown in COVID-19...

COVID-19 is a highly contagious viral infection caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which is declared pandemic by the World Health Organization (WHO). The spike protein of SARS-CoV-2 is a key component playing a pivotal role in facilitating viral fusion as well as release of genome into the host cell. Till date t...

This article is an attempt to present a popular account of some of our work on Quantum Computation, Consciousness, Biological organisms and Universe done in last decade. We first discuss whether quantum mechanisms are significant in biology. Then we explain quantum computation, and relation of quantum computation with consciousness. Subsequently we...

Finding its way into the agricultural lands through various sources Cadmium (Cd) has been proved to be deleterious in plant systems. Cd-induced oxidative stress tampers with both plant's physiology and biochemistry. Nitric oxide (NO) is a key signaling molecule in plant stress response and plays a significant role in many regulatory pathways of pla...

The WRKY family of transcription factors modulates the host defense mechanisms in response to various environmental stresses. The role of WRKY33 in plant defense and its crosstalk with defense hormone was well established in Arabidopsis but very few information was noted in Sinapis alba. The present study was carried out in 2017, in which computati...

The pattern of interactions between foot and mouth disease (FMD) viral protein 1 (VP1) with susceptible and resistant host integrins were deciphered. The putative effect of site-directed mutation on alteration of interaction is illustrated using predicted and validated 3D structures of VP1, mutated VP1 and integrins of Bos taurus, Gallus and Canis....

Terminal heat stress has detrimental effect on the growth and yield of wheat. Very limited information is available on heat stress-associated active proteins (SAAPs) in wheat. Here, we have identified 159 protein groups with 4271 SAAPs in control (22 ± 3 °C) and HS-treated (38 °C, 2 h) wheat cvs. HD2985 and HD2329 using iTRAQ. We identified 3600 pr...

Cadmium is a well known toxic heavy metal, which has various detrimental effects on plant system. In plants an important enzyme involved in the production of nitric oxide, nitrate reductase, is also affected by cadmium toxicity. According to many studies cadmium has an inhibitory effect on nitrate reductase activity. Similar effect of cadmium was f...

Cadmium is a well known toxic heavy metal, which has various detrimental effects on plant system. In plants an important enzyme involved in the production of nitric oxide, nitrate reductase, is also affected by cadmium toxicity. According to many studies cadmium has an inhibitory effect on nitrate reductase activity. Similar effect of cadmium was f...

Abiotic stress is one of the major factors responsible for huge yield loss in crop plants. MicroRNAs play a key role in adaptive responses of plants under abiotic stress conditions through post-transcriptional gene regulations. In present study, 95 potential miRNAs were predicted in Brassica juncea using comparative genomics approach. It was noted...

Enriched pathway (A) starch and sucrose metabolism, (B) pentose and glucuronate interconversions for target genes for predicted miRNA in B. juncea.

Gene ontology (A) biological process, (B) cellular component, (C) molecular function enrichment analysis of target genes for predicted miRNA in B. juncea. Darker the color more significant is the term.

Distribution of simple sequence Repeats (SSRs) on (A) miRNA precursor sequences and (B) target genes in B. juncea.

Present study was aimed to identify mutations/SNPs and predict microRNAs within the 3'untranslated region of bovine Hsp90 AA1 gene. A total of 28 mutations were identified in the 3'untranslated region of bovine Hsp90 gene among 4 indigenous zebu breeds and 1 crossbred population. Results revealed that, natural mutational changes are more in crossbr...

MicroRNAs (miRNAs) are ~22nt long non-coding RNAs, which regulate the gene regulation at the post transcriptional level in both plants and animals. These miRNA are conserved in nature and hence potential base for new miRNA prediction through homology search. No miRNAs in this species are identified so far in economically important water buffalo (Bu...

Role of salicylic acid in plants grown under metal contaminated soil is not yet understood. To investigate if signaling by Cd-induced H2O2 or SA, collide or integrate, rice cv. HUR3022, grown for 3, 7 and 10 day under 50 µM Cd/SA alone or in combination were studied. Dynamic distribution of Cd2+ and SA, cell viability, time-dependent changes in H2O...

Rice seedlings grown under 50 μM cadmium alone or in combination with 5 μM methyl jasmonate were investigated for Cd-induced oxidative injury at 3, 7 and 10 days of treatment. MeJA treatments alone did not have any significant change in antioxidant enzyme activities or levels of H2O2 and O2− in roots/shoots, as compared to controls during 3–10 days...

ZAT12 a C2H2-zinc-finger protein is an abiotic stress-responsive transcription factor in plants having less information about their structure. Transcription analysis proved that ZAT12 transcripts over-expressed during drought, heat and salt stress conditions which led to an interest in 3-D structural studies of ZAT12in Brassica carinata. Over-expre...

Binding interactions of cadmium (Cd) with rice ascorbate peroxidase (OsAPX) in presence or absence of jasmonate was examined in-silico. OsAPX is a 250 amino acid long protein with 90 % sequence similarity to soybean-APX. The 3D model of OsAPX obtained by homology modeling using soybean APX (PDBID:1OAF) as template was associated with -15975.85 kJ/m...

Enhanced disease susceptibility 1 (EDS1), a plant-specific protein has homology with the eukaryotic lipase in their N-terminal halves and a unique domain at its C-termini. EDS1 is known to be an important regulator of biotic stress and an essential component of basal immunity. EDS1 interacts with its positive co-regulator phytoalexin deficient 4 (P...

Knowledge of rice genome brings new dimensions to the management of abiotic stresses; however, gene sequences in the rice genome are yet to be assigned structure and function. Hydrogen peroxide, salicylates and jasmonates act as signal molecules in plants employing common machinery to manage abiotic stress. The present work is primarily focused to...

Rice cv. Bhalum-I is widely cultivated in north-east India. It has high amount of peroxidase (POX) in its roots, which can be a promising source for the industrial production of this enzyme. The initial enzyme isolation included its homogenization and extraction from the roots of 15 day old rice (Oryza sativa L.) seedlings cv. Bhalum-1. Purificatio...

Rice cv. Bhalum-I is widely cultivated in north-east India. It has high amount of peroxidase (POX) in its roots, which can be a promising source for the industrial production of this enzyme. The initial enzyme isolation included its homogenization and extraction from the roots of 15 day old rice (Oryza sativa L.) seedlings cv. Bhalum-1. Purificatio...

Plants may obtain phosphorous via symbiotic association with arbuscular mycorrhizal (AM) fungi like Glomus mossae that facilitate the uptake of Pi by plants and in exchange obtain carbohydrate, representing a large sink for atmospheric plant fixed CO 2. The Pi transporters (PhT) contain proton coupled motifs that are important for transport of phos...

Plants may obtain phosphorous via symbiotic association with arbuscular mycorrhizal (AM) fungi like Glomus mossae that facilitate the uptake of Pi by plants and in exchange obtain carbohydrate, representing a large sink for atmospheric plant fixed CO 2. The Pi transporters (PhT) contain proton coupled motifs that are important for transport of phos...