Content uploaded by Imad Hadi Hameed
Author content
All content in this area was uploaded by Imad Hadi Hameed on Mar 05, 2019
Content may be subject to copyright.
A preview of the PDF is not available
Daucus carota : In vitro Antimicrobial Activity and Bioactive Compounds of Methanolic Fruit Extract Using FTIR Spectroscopic Analysis
Abstract
Plant showed nutritional and therapeutic benefits including antimicrobial, antioxidant, anticancer, hypolipidemic, cardiovascular, central nervous, respiratory, immunological, anti-inflammatory, analgesic antipyretic and many other pharmacological effects. Phytochemical analysis showed that the root of Daucus carota contained alkaloids, carbohydrates, chlorogenic acid, flavonoids, phenols, essential oil, terpenoid and coumarin. The objectives of this study were analysis of the secondary metabolite products using Fourier-transform infrared spectroscopic profile and evaluation of its anti-microbial activity. In the current study, the anti-microbial activity of Daucus carota methanolic extract was evaluated by determining the zone of inhibition against nine bacteria and eight fungi and yeast. Maximum zone formation was against Staph aureus (4.17±0.15) and very highly active against Aspergillus flavus (4.59±0.16). The FTIR analysis of Daucus carota proved the presence of alkyl halides, Alkenes, Aromatic and Amide which shows major peaks at 667.37, 873.75, 921.97, 1026.13, 1139.93, 1234.44, 1317.38, 1379.10, 1415.75, 1519.91, 1598.99, 1740.72, 2852.72, 2922.16, 3223.05, and 3265.49. © 2019, Indian Journal of Public Health Research and Development. All rights reserved.
... Together, these peaks represent the polysaccharide characteristics peak. A ketone and an amine produced notable bands at 1612 cm −1 and 3313 cm −1 , respectively [45]. ...
Until now, the Mauritian endemic fruit Tambourissa ficus of the Monimiaceae family has
remained unexplored. The study’s goal was to look into the phytochemical composition and antioxidant properties of different solvent extracts of the fruit. The presence of phenolics, flavonoids,
terpenes, coumarins, alkaloids, and tannins was discovered through qualitative screening. The
highest total polyphenol content (TPC = 9.78 ± 0.18 mg GAE/g dw) and the highest total flavonoid
content (TFC = 8.84 ± 0.07 mg QE/g dw) was observed in ethanolic extract, while the highest total
terpenoid content (TTC = 587.9 ± 0.72 mg linalool/g dw) was found in the acetone extract. The
antioxidant activity vs. ABTS was the highest (4.71 ± 0.18 mg TE/g dw) in the ethanol extract. All
three groups—TPC, TFC, and TTC revealed a moderate correlation with ABTS antioxidant activity,
being 0.754, 0.778, and 0.774 on average, respectively. Ultraviolet–visible (UV-Vis) spectroscopy
spectrophotometry and Fourier transform infrared spectroscopy (FTIR) spectroscopy confirmed
the presence of polyphenolic compounds. Individual noteworthy phytochemicals, including the
alkaloids chelidonine, protopine, and brevicarine, which are potential antioxidant compounds, were
also discovered in the fruit through liquid chromatography–mass spectrometry (LC-MS) screening.
The overall antioxidant activity of the fruit can, therefore, be attributed to the synergistic effects of
the multiple chemical components in the extracts.
Background and Objectives Antihistamine drugs for acute upper respiratory tract infection treatment in children have many side effects. In this study, a anti-cough syrup was prepared from Cordia (Sepestan in Persian) extract and its effect was compared to that of diphenhydramine syrup on cough relief of children with upper respiratory tract infection. Subjects and Methods This double-blind randomized clinical trial was conducted on 124 children with upper respiratory tract infection aged 6-12 years referred to Abouzar Hospital in Ahvaz, Iran during 2019- 2021 in two treatment groups. The first group was given Sepestan syrup at a dose of 20 mg/kg/day and the second group received diphenhydramine syrup at a dose of 5 mg/kg/day in four dosages. For three days, the number and severity of day-time and night-time coughs were examined and the data were analyzed with independent t-test and chi-square test in SPSS software, version 24. Results The number and severity of day-time and night-time coughs in the group received Sepestan syrup significantly decreased compared to the diphenhydramine group, without any side effects (P<0.001). Conclusion High polysaccharides, strong antioxidants, and anti-inflammatory compounds of Sepestan can reduce throat irritation and soften the chest. The mucilage in the Sepestan extract can dissolve in water and produce a viscous substance such as gelatin that cause softening of the breast and expectoration.
ResearchGate has not been able to resolve any references for this publication.