Ilya A. Mikhailov

Center for Expertise and Quality Control of Medical Care of the Ministry of Health of Russian Federation

Background: Unified system for assessing the results and real contribution of of telemedicine consultations (TMC) to improving the quality of medical care in the healthcare system of the Russian Federation has not yet been developed. Aim: Development of a system of indicators for differentiated assessment of the need for TMC in the provision of med...

In 139 patients with verified gastric cancer, the infiltration of the postoperative material with CD8+ cells was analyzed. Automated morphometric analysis of immunostained slides was performed separately in different specimen sites (tumor center, invasive edge, and peritumoral mucosa). The mean area of infiltrating CD8+ cells in the tumor center an...

The use of modern approaches based on convolutional neural networks (CNNs) for segmentation of whole slide images (WSIs) helps pathologists obtain more stable and quantitative analysis results and improve diagnosis objectivity. But working with WSIs is extremely difficult due to their resolution, size and the presence of a large number of incompati...

Aim. Integral rating system approbation of the Russian Federation regions using weight coefficients based on indicators characterizing the effectiveness of the organization of medical care in cardiovascular surgery. Methods. The study included 6 indicators with the highest final rating and expert agreement coefficient (based on data from the previo...

Objective : development of approaches to predict the likelihood of adverse reactions (ARs) when using drugs based on a comprehensive assessment of risk factors. Material and methods . We used a database containing 1,450 drug-related ARs reports from January through December 2021. A list of antibacterial drugs by international nonproprietary name (I...

The adaptation of methodology of expert selection of indicators for integral evaluation of efficiency of organization of medical care on profile "pediatrics" at the level of the Subject of the Russian Federation. The study was organized as expert survey using structured interview technique. At the first stage, primary selection of indicators carrie...

Cancer-associated fibroblasts (CAFs) — mesenchymal cells in the tumor stroma, play one of the leading roles in tumor progression in many different tumors, including colorectal cancer. Scientists have described many markers for CAFs, but none of them is specific. We performed immunohistochemistry tests using five antibodies (αSMA, POD, FAP, PDGFRα,...

Aim of the study - determination of approaches to the formation of the rating of the constituent entities of the Russian Federation, reflecting the state of medical care in various profiles (the effectiveness and quality of medical care in various profiles). The study included a comprehensive analysis of the structure and content of the reports bas...

Introduction. A clinical and economic study was carried out to assess the effectiveness of infertility treatment using assisted reproductive technology programs with preimplantation genetic testing (PGT) in patients with various reproductive disorders. Materials and methods. Twenty models representing discrete-time Markov processes are developed....

AIM : Formation of a system of target indicators for rating and integral assessment of the effectiveness and efficiency of the organization of medical care for patients with diabetes mellitus in the Russian Federation regions. MATERIALS AND METHODS : The study was an expert survey in the form of a structured interview. At the first stage, we perfor...

Objective: Assessment of the incidence of EBV-associated gastric adenocarcinomas in a sample of Russian patients, as well as clarification of the clinical and morphological characteristics and median survival of patients with EBV-associated gastric cancer. Material and methods: We used samples of surgical material from 282 patients with a verifi...

Background: More than 80% of pathologists suffer from various manifestations of disorders of the musculoskeletal system. The daily duties of pathologists include several hours of work at a microscope and a computer. Microscopes are often not optimized for ergonomics and do not allow the doctor to take the correct sitting position, most often worki...

Objective: Development of original methodological approaches to annotation and labeling of histological images in relation to the problem of automatic segmentation of the layers of the stomach wall. Material and methods: Three image collections were used in the study: NCT-CRC-HE-100K, CRC-VAL-HE-7K, and part of the PATH-DT-MSU collection. The us...

Background: Not everyone with CML responds optimally to TKI-therapy, especially those with BCR::ABL1T315I. PF-114 is a 4 th-generation oral tyrosine kinase-inhibitor (TKI) active against wild-type and mutated BCR::ABL1 isoforms including BCR::ABL1T315I. We present final results of a phase-1 study (NCT02885766). Methods: 3+3 dose-escalation study to...

Background. Diabetes mellitus (DM) is a significant predictor of atherosclerosis, cardiovascular disease, and cardiovascular mortality. It is known that atherosclerosis occurs earlier in patients with diabetes, reducing the duration of their life. Leptin as well as other inflammatory markers can contribute to the progression of atherosclerosis in p...

The aim of the study was to study and evaluate the predictive value of the immune microenvironment of gastric cancer and morphologically normal mucous membrane of the peritumoral area using an automatic morphometric analysis system on the example of CD 8+ cells. Material and Methods . Surgical samples from 130 patients with a verified diagnosis of...

The aim of the study: Is to establish the relationship between the persistence of viral antigens of the Epstein-Barr virus (EBV) and the cellular composition of the immune microenvironment of tumor tissue and the mucous membrane of peritumoral area in gastric cancer. Material and methods: We used samples of surgical material from 55 patients wit...

Objective: To determine the level of E-cadherin expression in tumor emboli, to compare it with expression in a tumor, to determine the dependence of E-cadherin expression in tumor emboli on the clinical and morphological characteristics of gastric cancer. Material and methods: We used samples of surgical material from 280 patients with a verifie...

Introduction: Tumor budding was declared as independent prognostic factor for early cancer in 2016. Tumor-associated fibroblasts (CAFs) are one of the main components of tumor microenvironment. Plenty of different markers are used for detection of CAFs, including podoplanin (POD). Objective: The aim of this study is to identify correlation betwe...

Automatic layers recognition of the wall of the stomach and colon on whole slide images is an extremely urgent task in digital pathology as it can be used for automatic determining the depth of invasion of the digestive tract tumors. In this paper we propose a new CNN-based method of automatic tissue type recognition on whole slide histological ima...

Introduction. Immunohistochemical staining by an indirect method with a chromogenic label requires en-zymes, among which is alkaline phosphatase (AP). AP is also present in human tissues. It can break down the molecules of the immunohistochemical substrate, which leads to significant background staining. It is necessary to block endogenous enzymes...

Carcinogenesis and tumor progression are not caused not only by malignant epithelial cells, but also by the tumor stroma around cancer stem cells which performs regulatory, nutritional and 'framework' functions. It is represented by mesenchymal cells of various types predominantly by cancer-associated fibroblasts (CAF). αSMA, FAP-1, desmin, podopla...

Background: PF-114 is a 4th-generation oral tyrosine kinase-inhibitor (TKI) active against wild-type and mutated BCR-ABL1 isoforms including BCR-ABL1T315I. We present data from a phase-1 study in patients with chronic or accelerated phase chronic myeloid leukaemia (CML) failing ≥2 TKIs or with BCR-ABL1T315I (NCT02885766) with ≥6 months therapy. Met...

Mucous glands is an important diagnostic element in digestive pathology. The first step of differential diagnosis of colon polyps in order to assess their malignant potential is gland segmentation. The process of mucous glands segmentation is challenging as the glands not only needed to be separated from a background but also individually identifie...

EBV-associated gastric adenocarcinoma accounts for 10% of all gastric adenocarcinomas. The main known facts about the pathogenesis of EBV-associated gastric adenocarcinoma are presented. There are two main morphological types: gastric carcinoma with lymphoid stroma - GCLS (including lymphoepithelioma-like carcinoma; carcinoma with Crohn's disease-l...

Objective: To study the relationship of LMP-1 expression with the clinical and morphological characteristics of gastric cancer and to study LMP-1 expression in the glandular epithelium of the peritumoral tissue. Material and methods: Samples of gastric cancer surgical material from 67 patients were included in this study. Primary mouse monoclona...

Background: PF-114 mesylate is a 4th-generation oral tyrosine kinase-inhibitor (TKI) active against wild-type and mutated BCRABL1 isoforms including those with a BCRABL1T315I. We present data from a phase-1 study in subjects with chronic or accelerated phase chronic myeloid leukaemia (CML) failing ≥2 TKIs or who have BCRABL1T315I (NCT02885766). Me...

Key Clinical Message I report a novel BCR‐ABL point mutation c.844G>C p.E282Q and a case of combination of two BCR‐ABL point mutations (p.E282Q and p.L298R) and exon 7 deletion (del. c.1086‐1270) in TKI‐resistant patient. These point mutations were present only in a truncated transcript and were absent in “wild‐type” BCR‐ABL transcript.

Mucous glands lesions analysis and assessing of malignant potential of colon polyps are very important tasks of surgical pathology. However, differential diagnosis of colon polyps often seems impossible by classical methods and it is necessary to involve computer methods capable of assessing minimal differences to extend the capabilities of the cla...

Mucous glands lesions analysis and assessing of malignant potential of colon polyps are very important tasks of surgical pathology. However, differential diagnosis of colon polyps often seems impossible by classical methods and it is necessary to involve computer methods capable of assessing minimal differences to extend the capabilities of the cla...