Hassan Korbekandi

Dr Korbekandi Pharmacy

Interferon β (IFNβ) is the most prescribed drug that has been used frequently for the treatment of multiple sclerosis (MS) patients. The aim of this study is to improve the production of IFNβ by induction of site directed mutagenesis. Accordingly, recombinant constructs were designed in order to enhance the expression of IFNβ mRNA and protein. The...

Interferon β (IFNβ) is the most important drug that has been used frequently for multiple sclerosis treatment. This study has tried to improve the IFNβ production by introducing mutations in the coding region of IFNβ, while its amino acid sequence is intact. Two recombinant vectors IFNβK and IFNβK+CRID were designed by site-directed mutagenesis. Th...

Increasing pressure to develop green and eco-friendly methods for synthesizing nanoparticles has provoked researchers to shift to microorganisms and biological systems. Plants can be used in large-scale production of nanoparticles, and in order to improve their potential in nanoparticle synthesis, it is necessary to investigate the biochemical and...

Context: There is an ever-growing need to develop green, non-toxic, and eco-friendly procedures for synthesis and assembly of nanoparticles (NPs) with the desired morphologies and sizes. The hydroalcoholic extract of Persian oak leaves [Quercus brantii Lindl. (Fagaceae)] contains high content of phenolic and flavonoid compounds with strong antioxid...

The objectives of this paper were production of probiotic yoghurt using L. paracasei ssp. tolerans, and evaluation of pH changes, viable counts, and organoleptic properties of the produced yoghurt. It was observed that the pH decrease during the fermentation period was faster in the milk inoculated with L. paracasei ssp. tolerans plus starter cultu...

Fermented milk products containing probiotics and prebiotics can be used in management, prevention and treatment of some important diseases (e.g., intestinal- and immune-associated diseases). Microencapsulation has been used as an efficient method for improving the viability of probiotics in fermented milks and gastrointestinal tract. Microencapsul...

Metal and metal oxide nanoparticles have been used in different fields of medicine, pharmacy, various industries, animal husbandry, packaging, accessories, cosmetics, health and military uses. Unfortunately, most of the synthetic methods are expensive, toxic and unecofriendly, and thus there is an ever-growing need for "green chemistry" to use bio-...

The objectives were to study the potential of Pulicaria gnaphalodes (Vent.) Boiss. aerial parts in production of nanoparticles and the effect of the extraction solvent on the produced nanoparticles. Methanol and dichloromethane extracts were prepared by percolation of the plant powder. Both the extracts of P. gnaphalodes (Vent.) Boiss. successfully...

Green synthesis of nanoparticles (NPs) using microorganisms is ecofriendly and gives researchers greater opportunities in sizes and shapes of the synthesized NPs. The aims of this research were the biosynthesis of silver NPs by biotransformations using Saccharomyces boulardii and analysis of the sizes and shapes of the produced NPs. Dried (commerci...

The objectives of this study were the biosynthesis of silver nanoparticles (NPs) by biotransformations using Saccharomyces cerevisiae and analysis of the sizes and shapes of the NPs produced. Dried and freshly cultured S. cerevisiae were used as the biocatalyst. Dried yeast synthesized few NPs, but freshly cultured yeast produced a large amount of...

The objectives were to study the potential of Azolla pinnata whole plant in green synthesis of nanoparticles (NPs), and the effect of the extract ethanol concentration on the produced NPs. Azolla pinnata whole plants were freshly collected, air dried at room temperature, powdered, and sieved. Seventy and 96 percent hydroalcoholic extracts were prep...

The objectives were to study the potential of Althaea officinalis radix in production of silver NPs, and the effect of the extract ethanol concentration on the produced NPs. Seventy and ninety-six percent hydroalcoholic extracts were prepared by percolation of the plant powder. The extract was concentrated by rotary evaporator and then freeze-dried...

Silver nanoparticles (NPs) have been the subjects of researchers because of their unique properties (e.g., size and shape depending optical, antimicrobial, and electrical properties). A variety of preparation techniques have been reported for the synthesis of silver NPs; notable examples include, laser ablation, gamma irradiation, electron irradiat...

By the current study, we tried to find out the interactive mechanisms enrolled by Hsp70 and Hsp90 following the 6-hydroxydopamine (6-OHDA)-induced oxidative stress. Of heat shock protein (Hsp) family, we have previously evaluated the effects of Hsp90 gene silencing on in vitro model of Parkinson's disease and its influence on controlling the mechan...

For the past 30 years, bacterial L-asparaginases have been used as therapeutic agents in the treatment of acute childhood lymphoblastic leukemia. It is found in a variety of organisms such as microbes, plants and mammals. Their intrinsic low-rate glutaminase activity, however, causes serious side-effects, including neurotoxicity, hepatitis, coagulo...

This chapter reviews the green synthesis of nanoparticles using algae. Specifically, the chapter covers the following topics: important aspects in the biosynthesis of nanoparticles; biosynthesis of nanoparticles using algae; and extraction of biosynthesized nanoparticles.

Protein contact map is a simplified representation of a protein's spatial structure. The Committee Machine is a machine learning method that allots the learning task to a number of learners and divides the input space into subspaces. Learners' responses to an input are combined to produce the system's final response, which is more accurate than any...

Silver nanoparticles are increasingly used in various fields of biotechnology and applications in the medicine. Objectives of this study were optimization of production of silver nanoparticles using biotransformations by Fusarium oxysporum, and a further study on the location of nanoparticles synthesis in this microorganism. The reaction mixture co...

The production of nanoparticles using a biosystem is considered green chemistry. Application of plant extracts as a biological process has been proven to be suitable for synthesis of nanoparticles. This study designed in order to evaluate the production of silver nanoparticles using Juglans regia leaf extract and to compare the outcome of different...

BACKGROUND: The objectives of this study were optimization of silver nanoparticle synthesis using biotransformations by Lactobacillus casei subsp. casei, and studying the location of nanoparticles synthesis in this microorganism. RESULTS: The presence of AgNO3 (0.1 mmol L−1) in the culture as the enzyme inducer, and glucose (56 mmol L−1) as the el...

Entrapment of permeabilized whole cells within a matrix is a common method for immobilization. Chitosan possesses distinct chemical and biological properties, which make it a suitable matrix for entrapment and immobilization of penicillin G acylase (PGA). In the first step, Escherichia coli (ATCC 11105) cells were permeabilized using N-cetyl-N,N,N-...

The clavulanic acid regulatory gene (claR) is in the clavulanic acid biosynthetic gene cluster that encodes ClaR. This protein is a putative regulator of the late steps of clavulanic acid biosynthesis. The aim of this research is the molecular cloning of claR, isolated from the Iranian strain of Streptomyces clavuligerus (S. clavuligerus). In this...

Background: Colorectal cancer is the third cause of cancer death in western countries. Age, inadequate diet, obesity, inactivity and genetic changes are some of the risk factors of colorectal cancer. Corelation of genetic diversity in homologous recombination repair system with cancer was evaluated in many recent studies. This study was done to inv...

Nowadays, fermented milk products such as yoghurt, kefir, buttermilk, and acidophilus milk are gaining increasing attention. Fermented milks containing probiotics and prebiotics could be used to prevent and/or cure some important diseases (such as intestinal- and immune-associated diseases). It is a goal of much interest to produce the products wit...

Clavulanic acid is produced industrially by fermentation of Streptomyces clavuligerus and researches have increased its production by strain improvement, recombinant DNA technology, and media composition and growth condition optimization. The main objective of this study was to increase the level of clavulanic acid production from Streptomyces clav...

Recent developments in the biosynthesis of nanomaterials have demonstrated the important role of biological systems and microorganisms in nanoscience and nanotechnology. These organisms show a unique potential in environmentally friendly production and accumulation of nanoparticles with different shapes and sizes. Therefore, researchers in the fiel...

Skimmed milk was inoculated with the commercial starter and Lactobacillus casei ssp. casei. pH changes, viable counts, and organoleptic properties of the produced control and probiotic yogurts were analysed. The pH decrease during the fermentation period was faster in the milk inoculated with L. casei plus starter. The growth of both starters in pr...

Peptostreptococcus productus U-1 was grown in a fructose-limited chemostat with caffeate (D = 0.19 h−1), and washed, harvested cells were used to reduce (E)-2-nitro-1-phenyl-1-propene 1a and (E)-2-nitro-1-phenyl-1-butene 1b in a two-liquid phase reaction system. The aminoalkanes 3a and 3b were formed in 47% and 7.5% yield, respectively, by reductio...

We aimed to optimize esterase production of Candida rugosa lipase (CRL). Active culture of C. rugosa (DSM 2031) was revived and the culture medium containing the most frequently used ingredients was optimized using a fraction of factorial design method, Taguchi. Temperature and pH of the culture was also optimized using one factor at a time method....