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G.R.Rout, S.Samantaray and P. Das (2000) In vitro manipulation and propagation of medicinal plants. Biotechnology Advances, 18:91-120. Canada
... In addition, the average number of shoots obtained (2 per plant or less), is low and the best results were obtained with sprouting treatment 4 and 2, with 0.5 and [16]. ...
... Clearly, at this stage of method development cell suspensions are not a good source of this main active compound. Rout et al. (2000) noted that the accumulation of secondary metabolites during in vitro culture depends on the composition of the culture medium, including type and amount of growth regulators, minerals and carbon sources as well as ambient conditions, temperature, light and gas composition during cultivation. A variety of physical and chemical conditions besides light could be changed to improve the in vitro production of betulinic acid in future work. ...
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