Guzel Burkhanova

Ufa Scientific Center of the Russian Academy of Science | USC RAS · Institute of Biochemistry and Genetics

Beneficial endophytic bacteria can suppress the development of insect pests through direct antagonism, with the help of metabolites, or indirectly by the induction of systemic resistance through the regulation of hormonal signaling pathways. Lipopeptides are bacterial metabolites that exhibit direct antagonistic activity against many organisms, inc...

Cytokinins (CKs) and abscisic acid (ABA) play an important role in the life of both plants and pathogenic fungi. However, the role of CKs and ABA in the regulation of fungal growth, development and virulence has not been sufficiently studied. We compared the ability of two virulent isolates (SnB and Sn9MN-3A) and one avirulent isolate (Sn4VD) of th...

The effect of chitosan conjugates with caffeic (ChCA) and ferulic (ChFA) acids in combination with Bacillus subtilis bacteria on the transcriptional activity of PR protein genes and proteome changes in potato plants during infection with Phytophthora infestans (Mont.) de Bary was studied. Plants grown from mini tubers of the Udacha variety were spr...

RNA interference is a gene silencing mechanism that plays an important role in genetic regulation in a number of eukaryotes. Argonaute (AGO) proteins are central to the complex RNA interference system. However, their role in this mechanism, both in the host plant organism and in the pathogen, has not yet been fully elucidated. In this work, we iden...

Bacillus subtilis 26D is a plant growth-promoting endophytic bacteria capable of inducing systemic resistance through the priming mechanism, which includes plant genome reprogramming and the phenomenon of RNA interference (RNAi) and microRNA (miRNAs). The phloem-feeding insect bird cherry-oat aphid Rhopalosiphum padi L. is a serious pest that cause...

Background: Currently, the role of microRNAs in plant immune responses is being actively studied. Thus, our aim was to research the effect of Stagonospora nodorum (Berk.) NEs SnToxA and SnTox3 on the expression of miRNAs involved in the wheat-S. nodorum interaction and to determine the role of phytohormones in this process. Methods: The expressi...

This review examines the properties of endophytic bacteria of the genus Bacillus as objects of biocontrol and ways to expand the spectrum of their protective action based on complexes with chitosan derivatives. The mechanisms of direct and indirect effects of bacteria on the protective potential of plants are described, the role of the pro-/antioxi...

The review examines the properties of endophytic bacteria of the genus Bacillus as objects of biocontrol, prospects to expand the spectrum of their protective action based on complexes with chitosan derivatives. The mechanisms of direct and indirect effects of bacteria on the protective potential of plants are described, the role of the pro-/antiox...

Phytophthora infestans is, worldwide, one of the main causal agents of epiphytotics in potato plantings. Prevention strategies demand integrated pest management, including modeling of beneficial microbiomes of agroecosystems combining microorganisms and natural products. Chitooligosaccharides and their derivatives have great potential to be used by...

Nitric oxide (NO) is a multifunctional, gaseous signaling molecule implicated in both physiological and protective responses to biotic and abiotic stresses, including salinity. In this work, we studied the effects of 200 µM exogenous sodium nitroprusside (SNP, a donor of NO) on the components of the phenylpropanoid pathway, such as lignin and salic...

Virulence factors of the pathogen Stagonospora nodorum Berk. are numerous necrotrophic effectors (NEs) (SnTox), which interact with the products of host susceptibility genes (Snn), causing the development of the disease. In this study, 55 accessions of bread spring and winter wheat were screened for sensitivity to NEs SnToxA, SnTox1, and SnTox3 usi...

28.07.2022 г. После доработки 02.09.2022 г. Принята к публикации 09.01.2023 г. Фитопатогенные грибы представляют угрозу продовольственной безопасности, ограничивая био-логический потенциал сельскохозяйственных культур и снижая качество получаемой продукции. На современном этапе разрабатываются методы защиты растений, основанные на естественном си-с...

Citation: Sorokan, A.; Burkhanova, G.; Gordeev, A.; Maksimov, I. Exploring the Role of Salicylic Acid in Regulating the Colonization Ability of Bacillus subtilis 26D in Potato Plants and Defense against Phytophthora infestans. Int. J. Plant Biol. 2023, 14, 242-253. https:// Abstract: Plant colonization by endophytic bacteria is mediated by differen...

The use of biocontrol agents based on endophytic bacteria against phloem-feeding insects is limited by a lack of knowledge and understanding of the mechanism of action of the endophyte community that makes up the plant microbiome. In this work, the mechanisms of the additive action of endophytic strains B. subtilis 26D and B. subtilis 11VM on the r...

Detection of specific microRNA (miRNA) is of great demand due to their essential role in genes regulation, stress response and development of diseases. However, mature miRNAs are small molecules that make it difficult to use routine amplification-based methods. Here, we report an approach for detection of miRNA based on a new type of isothermal amp...

Viral diseases cause the significant loss of crop yield and a marked deterioration of its quality annually. The main directions of plant protection against viral infections are: the rehabilitation of seeds by isolating and cultivating the apical meristem in vitro, the generation of transgenic plants resistant to viral infections using genes of spec...

Bacillus thuringiensis are the most common biological insect pest control agents. Some strains of B. thuringiensis are capable of endophytic existence, but the mechanisms of their interaction with plants and the role of phytohormones in this, for example, salicylic acid, which plays a key role in the development of plant immune responses against pa...

Endophytic plant-growth-promoting microorganisms can protect plants against pathogens, but they have rarely been investigated as potential biocontrol agents and triggers of induced systemic resistance (ISR), regulated by phytohormones, against viruses. We studied the role of endophytic strains Bacillus subtilis 26D and B. subtilis Ttl2, which secre...

In the last decade, works have appeared that propose the use of wheat susceptibility genes to the pathogen Stagonospora nodorum Berk. (Snn) as molecular markers for marker-based selection. Relationships in the pathosystem "wheat - S. nodorum" are carried out according to the "gene-for-gene" type in mirror image, i.e. the interaction of necrotrophic...

Cereal aphids (Aphididae) are considered one of the main pests of wheat. Recently, studies have shown that many members of the genus Bacillus can effectively reduce the pest number on plants. The lipopeptide production of bacteria may be involved this process. We have identified the surfactin-synthetase gene in strain B. subtilis 26D and isolates B...

We studied the effect of signaling molecules – stress phytohormones of salicylic (SA) and jasmonic (JA) acids, on the resistance of calluses and potato tubers to late blight pathogen – oomycete Phytophthora infestans (Mont.) De Bary and the pathogen’s ability to synthesize and secrete proteolytic enzymes that cause it properties. The introduction o...

Reactive oxygen species (ROS) play a central role in plant immune responses. The most important virulence factors of the Stagonospora nodorum Berk. are multiple fungal necrotrophic effectors (NEs) (SnTox) that affect the redox-status and cause necrosis and/or chlorosis in wheat lines possessing dominant susceptibility genes (Snn). However, the effe...

Plant pests and pathogens, including viruses, reduce the biological potential of crops and undermine food safety. The methods of crop protection based on natural systemic and cellular plant immunity are currently being developed and special attention is given to a unique mechanism known as RNA interference (RNAi). It creates one of the levels of ev...

Biocontrol agents based on strains of microorganisms that participate in mutualistic relationships with host plants, including those based on strains of endophytic bacteria, can be an alternative to chemical pesticides. There are endophytic B. thuringiensis strains that produce insectotoxic proteins and induce systemic resistance of plants to patho...

Ethylene, salicylic acid (SA), and jasmonic acid are the key phytohormones involved in plant immunity, and other plant hormones have been demonstrated to interact with them. The classic phytohormone cytokinins are important participants of plant defense signaling. Crosstalk between ethylene and cytokinins has not been sufficiently studied as an asp...

Вредители и патогены, в том числе и вирусы, ограничивают биологический потенциал сельскохозяйственных растений, что представляет огромную угрозу продовольственной безопасности. На современном этапе разрабатываются методы защиты растений, основанные на естественном системном и клеточном фитоиммунитете, где особое место занимает уникальный механизм,...

There was researched the effect of Bacillus subtilis bacteria in combination with salicylic (SA) and jasmonic (JA) acids on the state of the pro / antioxidant system (hydrogen peroxide content, catalase, peroxidase, superoxide dismutase activity) in connection with the development of potato resistance to late blight pathogen - oomycete Phytophthora...

In wheat, the level of expression of DCL4 and AGO1 genes correlated with resistance to the pathogen S. nodorum. The transcriptional activity of these genes increased when treated with strains of B. subtilis 26D and B. thuringiensis 11.

The effect of the Stagonospora nodorum effector SnTox3 on the biosynthesis and metabolism of cytokinins of host plant was studied. The SnTox3 effector influenced the biosynthesis of cytokinins along an ethylene-dependent and ethylene-independent pathway.

We studied the effect of the Bacillus bacteria on the content and activity of defensive compounds in potato plants upon infection with late blight pathogen. Bacterial treatment had a stimulating effect on the concentration of H2O2 and transcriptional activity of hydrolase inhibitor genes.

We have shown the direct and indirect effect of lipopeptide-producing endophytic bacteria of the genus Bacillus on the viability of greenbug aphid Schizaphis graminum Rond. and the induction of systemic resistance in wheat plants.

The effect of three concentrations of trans-zeatin on the indices of wheat plant resistance to S. nodorum was studied. Two concentrations of trans-zeatin showed a maximum increase in the resistance of wheat plants to S. nodorum.

It was shown that the interactions of SnToxA-Tsn1, SnTox1-Snn1 in the pathosystem wheat-Stagonospora nodorum play an important role in the development of Septoria nodorum blotch and are aimed at manipulating of host redox-status.

An important role of surfactin synthesis by endophytic bacteria in protecting wheat against cereal aphids has been shown to manifest itself in a direct insecticidal effect and an indirect effect through the induction of systemic resistance in plants.

Reactive oxygen species (ROS) play a central role in plant immune responses. The most important virulence factors of Stagonospora nodorum are multiple fungal necrotrophic effectors (NEs) (SnTox) that affect the redox status and cause necrosis and/or chlorosis in wheat lines possessing dominant susceptibility genes (Snn). However, the effect of NEs...

Viral diseases and their damage causing significant loss to economically important crops have increased by several folds during the last decade. All the conventional approaches are not able to eradicate the viral infection. Therefore, there is a need to look for efficient and eco-friendly viral disease-preventive measures. The genomic material of t...

Viral diseases and their damage causing significant loss to economically important crops have increased by several folds during the last decade. All the conventional approaches are not able to eradicate the viral infection. Therefore, there is a need to look for efficient and eco-friendly viral disease-preventive measures. The genomic material of t...

Novel properties of a previously obtained Bacillus subtilis 26DCryChS strain are described. The B. subtilis 26DCryChS strain is able to produce Cry1Ia δ-endotoxin from B. thuringiensis B-5351 and to exist in internal plant tissues of potato plants in the same manner as the endophytic B. subtilis 26D source strain (487 ± 53 and 420 ± 63 CFU*103/g, r...

One of the main insect potato pests is the Colorado potato beetle (Leptinotarsa decemlineata Say). It contains some microbial associates which can affect diverse interactions between host plant and the herbivorous insect. Previously, the most common L. decemlineata microsymbiont isolated from anterior and posterior parts of beetle gut was defined a...

The review provides current information on bacterial lipopeptides, mainly those produced by representatives of the genus Bacillus, which play an important role in plant protection against fungi, bacteria, and viruses exhibiting fungicidal, antibacterial, antiviral, and insecticidal properties, as well as phytoimmunomodulating activity. The present...

The fungus Stagonospora nodorum Berk. is the causal agent of Septoria nodorum blotch (SNB) of wheat. The most important factor of virulence of the Stagonospora nodorum are multiple fungal necrotrophic effectors (NEs) coded by SnTox genes that interact with the matching products of host susceptibility genes (Snn). SnTox-Snn interactions are mirror i...

В обзоре приводятся современные сведения о липопептидах бактерий, преимущественно продуцируемых представителями рода Bacillus, играющих важную роль в защите растений от болезней грибной, бактериальной и вирусной этиологии, проявляющих фунгицидные, антибиотические, антивирусные и инсектицидные свойства, а также фитоиммуно-модулирующую активность. Пр...

The authors studied the effect of treatment with bacteria Bacillus subtilis Cohn (strains 26D) and B. thuringiensis Berliner (strain B-6066) on the hydrogen peroxide (H 2 O 2 ) content, the activity of hydrolytic enzymes and their protein inhibitors in potato plants (Solanum tuberosum L.) in connection with development of resistance to the late bli...

Bacillus subtilis 26D stimulated transcriptional activity of jasmonic acid biosynthesis genes and jasmonate-dependent protective genes, including anionic peroxidase involved in lignification, which increased their resistance to late blight pathogen.

The virulence of the causal agent of Septoria nodorum blotch of wheat pathogenic fungus Stagonospora nodorum Berk. is caused by the presence of necrotrophic effectors (SnTox). Isolates infect wheat varieties that carry susceptibility genes (Snn) corresponding to toxins in the genome. SnTox-Snn interactions are mirror images of classical gene-for-ge...

Stagonospora nodorum Berk. is the causal agent of Septoria nodorum blotch (SNB) of wheat (Triticum aestivum L.). It synthesizes host-specific necrotrophic effectors (NEs), which facilitate infection process and ensure virulence of pathogen on host plant with a dominant susceptibility gene. The interaction of virulence genes products of the NEs path...

Plant viruses are globally responsible for the significant crop losses of economically important plants. All common approaches are not able to eradicate viral infection. Many non-conventional strategies are currently used to control viral infection, but unfortunately, they are not always effective. Therefore, it is necessary to search for efficient...

The fungus Stagonospora nodorum Berk. is the causative agent of Septoria nodorum blotch (SNB) of wheat. The most important factors of Stagonospora nodorum virulence include numerous fungal necrotrophic effectors (NEs) encoded by SnTox genes. They interact with the matching products of host susceptibility genes (Snn). SnTox-Snn interactions are mirr...

The greenbug aphid Schizaphis graminum Rond. causes a significant loss of the grain harvest. Therefore, to improve plant resistance to aphids is one of the topical tasks. The problem of creating varieties resistant to phloem-feeding insects is quite urgent, since the mechanisms procuring the resistance of plants to insects are not fully understood....

Viral diseases annually cause significant crop losses and significantly reduce the quality of products, including potatoes, some of the most important crops. Currently, viruses cannot be controlled with chemical pesticides, since known antiviral compounds are teratogenic and hazardous to people’s health. Biocontrol agents based on endophytic microo...

Plant viruses have a significant negative impact on major cultures of agronomic importance provoking a wide range of symptoms, representing a serious threat to global food security. Subsistence crops are often infected with viruses that cannot be controlled with pesticides. Biopreparations based on endophytic microorganisms may be an alternative. T...

In our work, we studied the expression of genes encoding PR proteins and redox enzymes of wheat species Triticum aestivum L., T. monococcum L. and T. timopheevii Zhuk., infected by Schizaphis graminum Rond. It was detected that hydrogen peroxide performs an important signaling function in triggering defense reaction in the short-term response of pl...

The most important virulence factors of the Stagonospora nodorum are multiple fungal necrotrophic effectors (NEs) that cause necrosis and/or chlorosis on wheat lines possessing dominant susceptibility genes (Snn). One such NE is SnTox3, which evokes programmed cell death and leads to disease when recognized by the wheat Snn3-B1 susceptibility gene....

Viruses cause epiphytotics on all major cultures of agronomic importance, representing a serious danger to global food security. As strictly intracellular pathogens, they cannot be controlled chemically and prophylactic measures consist mainly in the destruction of infected plants and excessive pesticide applications to limit the population of vect...

It has been found that the bacteria Bacillus subtilis Cohn. (strain 26 D) and Bacillus thuringiensis Berliner (strains V-6066 and V-5689) are able to suppress the vital activity of the greenbug aphid Schizaphis graminum Rond. The protective effects of the studied Bacillus spp. strains consisted of direct aphicidal activity, and they indirectly indu...

Выделение высокоэффективных эндофитных штаммов бактерий рода Bacillus, характеризующихся свойством индуцировать защитный ответ растений против патогенов является новым направлением в индустрии биологических препаратов. Проведен скрининг эндофитных бактерий на наличие генов, ответственных за синтез основных классов липопептидов. В геноме B. subtilis...

Известно, что растения реагируют на атаку патогенов интенсивной генерацией активных форм кислорода (АФК) и продукция АФК контролируется редоксферментами и фитогормонами. Имеются данные об участии в процессе генерации АФК в растительно-микробном взаимодействии ферментов НАДФН-оксидазы, пероксидазы и оксалатоксидазы. Нами был показан значительный вк...

Выявлено, что предобработка листьев пшеницы липопептидами, полученными из штаммов B. subtilis Ts 8-2, 26 Д и 11ВМ в 1,5-2 раза повышает концентрацию перекиси водорода в растениях и дополнительно увеличивает концентрацию перекиси при инфицировании. Инфицирование непредобработаных липопептидами листьев при этом снижает количество перекиси водорода в...

The ability of B. subtilis 26D strains producing surfactin and B. subtilis 26D sfp- deficient in surfactin synthesis was studied to influence the growth of the phytopathogenic fungus St. nodorum, the area of infection of wheat leaves pretreated by these strains and expression of genes of wheat protective proteins: PR-6, PR-9 and LOX. It is shown th...

Plant viruses are globally responsible for the significant crop losses of economically important plants. All common approaches are not able to eradicate viral infection. Many non-conventional strategies are currently used to control viral infection, but unfortunately, they are not always effective. Therefore, it is necessary to search for efficient...

We studied the effect of processing stability inductors-salicylic (SA) and jasmonic (JA) acids on the activity of proteolytic and amylolytic enzymes and their protein inhibitors in calli and in-vitro potato plants Solanumtuberosum L. upon infection of late blight pathogen Phytophthorainfestans Mont. de Bary. It has been shown that treatment of SA a...

The role of the components of the NADPH oxidases cascade in the generation of reactive oxygen species (ROS) by the causal agent of leaf blotch (Stagonospora nodorum Berk.) in leaves of common wheat (Triticum aestivum L.) of cultivars contrast in the resistance to the pathogen was studied. Two peaks of hydrogen peroxide generation - 6 and 24 hours a...

The effect of chitooligosaccharides (ChOSs) with an acetylation degree (AD) of 65% and 30% on the formation of defense reactions of potato plants (Solanum tuberosum L.) against Phytophthora infestans (Mont.) De Bary is studied. The content of hydrogen peroxide, the activities of peroxidases, allene oxide synthase, and hydrolase inhibitors, and the...