Guy D'hallewin

Italian National Research Council | CNR

The genus Pyrus has a long history in Sardinia (Italy), where two wild pear species (P. spinosa Forssk. and P. pyraster (L.) Burgsd.) and Pyrus communis L. cultivars are extensively distributed. Even if neglected, these taxa represent well-adapted key resources for redesigning sustainable farming systems. This report aims at shedding light on the p...

Plantago major L. and Plantago lagopus L. are cosmopolitan species, belonging to the Plantaginaceae family, used in traditional and modern medicine. In this study, a phytochemical evaluation of different aqueous and ethanolic extracts of leaves and roots of both species from the region of Beja in Tunisia was performed. Some biological activities, i...

The residue of chestnut processing generates a large amount of waste material, a resource not adequately exploited. The antioxidant and antitumoral properties of cold and hot water extracts from discarded pericarp of four chestnut Sardinian accessions and one marron variety were studied. The antioxidant capacity of the extracts was determined by sp...

Physiological dormancy of Vitis vinifera seeds jeopardises breeding programs and biodiversity evolution. To increase the knowledge on dormancy breaking, seeds of white and black Sardinian grape cultivars (cvs) were exposed to different pre-germination treatments. To shed light on the physiological and structural factors involved in seed dormancy, t...

According to the WHO, the overall age-standardized cancer rate keeps declining, and the number of cases diagnosed each year increases, remaining among the leading causes of death in 91 out of 172 recorded countries. In this context, novel cancer prediction and therapeutic protocols are compulsory. The effect of a Stachys circinata L’Hér dichloromet...

Ethnopharmacological relevance A rising resort to herbal therapies in Crohn's disease (CD) alternative treatments has been recently observed due to their remarkable natural efficiency. In this context, the weed plant Ambrosia maritima L., traditionally known as Hachich el Aouinet in Algeria and as Damsissa in Egypt and Sudan, is widely used in Nort...

Local fruit cultivars may improve the originality of specialty beers both directly, by conferring peculiar tastes and flavors, and indirectly, as a reservoir of new starter strains. Accordingly, the fungal and bacterial communities of Bisucciu fruit, a Sardinian apricot cultivar used to produce a local fruit beer, were here investigated by culture-...

In this study, carbon screen-printed sensors (C-SPEs) were functionalized with a high reactivity carbonaceous material (HRCM) to measure the ascorbic acid (AA) concentration in fresh-cut fruit (i.e., watermelon and apple) with a low content of vitamin C. HRCM and the functionalized working electrodes (WEs) were characterized by SEM and TEM. The inc...

This work provides companies in the fresh-cut produce sector with an Ascorbate Bluetooth© Analyzer (ABA), a screen-printed sensor-based device for ascorbic acid (AA) detection, for quality control all along the supply chain. The amperometric detection of AA on fresh and fresh-cut parsley, under correct and incorrect storage temperature, allowed us...

In order to shed light on contamination risks along the ready-to-eat chain of fresh commodities by emerging foodborne pathogens, we investigated the biofilm development in vitro of two Pseudomonas aeruginosa strains on fresh-cut lettuce (Lactuca sativa L. var. Iceberg). The experiment was performed employing a floating bioreactor system where modif...

Pompia is a Citrus species belonging to Sardinian endemic biodiversity. Health benefits were attributed to its flavedo rind extracts and essential oils while the juice qualities have never been investigated. In this paper, the antioxidant, antimicrobial, and other biological properties of Pompia juice were studied. A combined LCMS/electrochemical/b...

Background: Given the increasing request for natural pharmacological molecules, this study assessed the antimicrobial capacity of Pistacia lentiscus L. essential oil (PLL-EO) obtained from the leaves of wild plants growing in North Sardinia (Italy) toward a wide range of periodontal bacteria and Candida, including laboratory and clinical isolates...

Given the increasing request for natural pharmacological molecules, this study assessed the antimicrobial capacity of Pistacia lentiscus L. essential oil (PLL-EO) obtained from the leaves of wild plants growing in North Sardinia (Italy), toward a wide range of periodontal bacteria and Candida including laboratory and clinical isolates sp., together...

Background: Given the increasing request for natural pharmacological molecules, this study assessed the antimicrobial capacity of Pistacia lentiscus L. essential oil (PLL-EO) obtained from the leaves of wild plants growing in North Sardinia (Italy), toward a wide range of periodontal bacteria and Candida including laboratory and clinical isolates s...

The Sardinian pear germplasm is an important resource of genetic diversity that can be used for increasing data on European pear germplasm, to optimise the efficiency of the association studies within the genome and to identify genomic regions that control the main horticultural traits. A set of 108 Sardinian pear genotypes, composed of 81 Pyrus co...

Existing therapeutic strategies for breast cancer are limited by tumor recurrence and drug-resistance. Antioxidant plant-derived compounds such as flavonoids reduce adverse outcomes and have been identified as a potential source of antineoplastic agent with less undesirable side effects. Here, we describe the novel regulation of fatty-acid synthase...

Fruit beers are a special type of beers made with fruit addition in the production process. They are becoming popular especially in the field of craft breweries. Their production can be influenced by the seasonality of fruit harvest and by the risk of contamination due to the presence of microorganisms on the fruit skin. The purpose of the present...

In view of the increasing interest in natural antimicrobial molecules, this study screened the ability of Thymus capitatus (TC) essential oil and Citrus limon var. pompia (CLP) extract as raw extracts or incorporated in vesicular nanocarriers against Streptococcus mutans and Candida albicans. After fingerprint, TC or CLP were mixed with lecithin an...

Grape leaves influence several biological activities in the cardiovascular system, acting as antioxidants. In this study, we aimed at evaluating the effect of ethanolic and water extracts from grape leaves grown in Algeria, obtained by accelerator solvent extraction (ASE), on cell proliferation. The amount of total phenols was determined using the...

The effect of oxalic acid (OA) treatment on visual properties and bioactive compounds of two green and one purple cultivars of fresh asparagus was investigated during twelve storage days at 5 °C. Cold storage and OA treatment positively affected the overall appearance of the investigated cultivars. Cut-end dehydration increased, all along the stora...

This study was designed to detect the anti-oxidant and anti-inflammatory effects of phenolic compounds in grape leaves Algerian variety (GLAV) on endothelial damage. The phenolics were identified by using HPLC/DAD/ESI-MS analysis. The research of the anti-oxidant and anti-inflammatory effects was conducted on mice through 15 days. Results showed hi...

Background: The nanoincorporation of the extract of Citrus limon (L.) Osbeck var. pompia into liposomes was aimed at improving its antioxidant and antibacterial effects. Methods: The extract of the rind of Citrus limon (L.) Osbeck var. pompia was obtained by maceration in ethanol, evaporation, and freeze-drying. The extract phytochemical fingerprin...

Objectives Objectives: In view of the increasing interest in developing antimicrobials of natural origin, this in vitro study aimed to screen the potential antimicrobial capacity of Pistacia lentiscus L. (PLL) essential oil against different bacterial species and to evaluate a potential citotoxicity to human oral cells. Methods Methods: PLL oil obt...

Antibiotic resistance is an increasing serious issue to global health. As an alternative to chemicals, the use of natural molecules have been proposed to reduce the cariogenicity of Streptococcus mutans, which virulence is increased by the presence of Candida albicans in the oral biofilm. The aim was to assess the antimicro- bial capacity of Thymus...

The aim of the work was to extract, characterize, and formulate Thymus capitatus (Tymbra capitata) essential oil in phospholipid vesicles: liposomes, glycerosomes and Penetration Enhancer-containing Vesicles (PEVs). The steam-distilled essential oil was mainly composed of carvacrol. The oil was mixed with lecithin and water to produce liposomes, or...

BACKGROUND: The present study was performed to clarify the strategies of Penicillium digitatum during pathogenesis on citrus, assessing, on albedo plugs, the effects of treatment with NaHCO3, at two different pH (5 and 8.3), on cell wall28 degrading enzymes activity, over a period of 72 h. RESULTS: The treatment with NaHCO3, under alkaline pH, dela...

An integrated device, for real-time monitoring of glucose and phenols absorption, that consists of a sensors/biosensors system (SB) and a Caco-2TC7 human intestinal cell culture, is shown here. The SB was made of a glucose oxidase-based biosensor, a sentinel platinum sensor, a laccase/tyrosinase-based biosensor and a sentinel carbon sensor located...

Pistacia lentiscus berry oil (LBO) represents a typical vegetal product of the Mediterranean basin that has been formally used in traditional cuisine for 100s of years. In addition to its interesting alimentary properties, this product could represent an interesting candidate in the field of research on the study of new anti-infective agents. In fa...

During the archaeological excavations in the Phoenician and Punic settlement of Santa Giusta (Oristano, Sardinia, Italy), dating back to the 6th–2nd centuries bc, several Prunus fruitstones (endocarps) inside amphorae were recovered. The exceptional state of preservation of the waterlogged remains allowed morphometric measurements to be done by ima...

To assess the biocompatibility and anti-bacterial activity of phytocomplex of Citrus lemon var. pompia (CLP) and essential oil of Thyme herba- barona Loisel (THB) in innovative phospholipid vesicles. CLP was obtained by the rind of fresh fruits using an ethanol/water mixture. THB was obtained by the aerial part of the plant by hydro distillation. T...

To evaluate the extension of sound surfaces (ICDAS 0), enamel (ICDAS 1–3) and dentine (ICDAS 4–6) carious lesions, filled, missing tooth surfaces by ICDAS of participants of a randomized clinical trial (NCT02698371). Cross-sectional observation of 38 voluntary participants of the double blind RCT approved by UFP Ethics Committee, National Clinical...

The identification of plum varieties is generally done on the base of distinctive plant traits such as shape, size, and fruit drupe color identified during the variety registration, following official descriptors. In this paper, image analysis techniques were applied to study endocarps variability of 23 Prunus domestica cultivars from Sardinia. Dig...

The pomaces from red grapes were used as a source of phenolic antioxidants, which are known to have health-promoting effects. Environmentally-friendly extraction strategies were investigated to improve the rate and recovery of an extract with high phenolic content and antioxidant activity, which were evaluated by the Folin-Ciocalteu, DPPH, ABTS(+),...

The effect of cold storage under oxygen-enriched atmosphere on nutritional quality of 'Sanguinello Comune' blood oranges was investigated. The fruit were kept for 40 days at 10°C in hermetically closed chambers continuously ventilated with atmospheric air (control) or with oxygen-enriched air containing 76kPa O2 (EnrO2). Superatmospheric oxygen cau...

La pubblicazione del quinto quaderno di “Frutti dimenticati e biodiversità recuperata” si inserisce tra le attività che ISPRA conduce, insieme ad alti enti di ricerca e agli agricoltori, per il recupero e il mantenimento del patrimonio rappresentato dalle numerosissime cultivar di frutta, olivi, viti, castagni e noccioli che generazioni di contadin...

The publication of the fifth book of "Frutti dimenticati e biodiversità recuperata" forms part of the activities that ISPRA leads , together with other research institutes and with farmers, for the recovery and preservation of the heritage represented by the many varieties of fruits, olives, vines, chestnut and hazelnut trees that generations of It...

The publication of the fifth book of "Frutti dimenticati e biodiversità recuperata" forms part of the activities that ISPRA leads , together with other research institutes and with farmers, for the recovery and preservation of the heritage represented by the many varieties of fruits, olives, vines, chestnut and hazelnut trees that generations of It...

The behavior of a Sardinian plum, cv. Meloni, was investigated with regard to short-heat treatments at 20, 50, 55 or 60°C in water with 0 or 2% NaHCO3 (SBC) for 0, 15, 30, 45 or 60 seconds. Fruits were stored for one month at 5°C and 95% RH followed by a simulated marketing period (SMP) at 20°C and 80% RH for six days. Quality and decay percentage...

Purpose of review: This review summarizes the latest scientific and technological findings on the use of UV radiation to increase the health-promoting properties of fruits and vegetables and provides some suggestions for future research.Main findings: UV treatment is considered an emerging technology for enhancing the health-promoting compounds in...

The objective of this research was to evaluate the natural resistance of different Sardinian pear germplasm accessions to P. expansum, the causal agent of postharvest blue mold. Experiments were carried out in vivo and in vitro with seven accessions of pears ('Sarmentina', 'Vacchesa', 'De Puleu', 'De su Duca', 'Natalina', 'Oliena' and 'Laconi5') be...

One of the main etiological agents of postharvest citrus decay is blue mould caused by Penicillium italicum. This pathogen is currently controlled by the application of synthetic fungicides. The use of postharvest chemicals is essential if fruit is destined for storage, since postharvest life would be significantly reduced. However, concerns regard...

Up to date, limited data are available about electromagnetic phase signaling effects on host-pathogen interactions during the postharvest of horticultural commodities. Inspired by the last striking works on water physics, quantum signaling through phase transfer and its impact on biological and histological structures, we studied the effect of diff...

Imazalil (IMZ) plays a key role in the storage and marketing of Citrus fruits. It represents a very important tool in the fight against postharvest pathogens and is an efficient system for reducing economic losses due to the development of rots from Penicillium digitatum and Penicillium italicum. However, the current technologies employed to apply...

A novel device for the study of antimicrobial activity by vapour contact of volatile substances have been designed. This "big size" system, made up in inert acrylic material, is furnished with a fan and a hot plate with the aim to have a quick evaporation of volatile substances. It is able to contain fruits or other food products under controlled a...

The effectiveness of ultraviolet-C light (UV-C; 254 nm) alone at 0, 3, 6 and 12 kJm(-2), or combined with 0.0 or 0.5% (w7v) sodium bicarbonate (SBC), to control plum (Prunus domestica cv Stanley) postharvest decay caused by Penicillium expansum L. and Botrytis cinerea was investigated. First, fruit was sanitized and in one experiment plums were art...

Penicillium expansum causes blue mould rot, a serious post-harvest disease of pome fruits and is the main producer of the mycotoxin patulin. The occurrence of natural resistance against different hostpathogens, has been evidenced in some pear accessions of the Sardinian germoplasm. The aim of this research was to correlate P. expansum growth and pa...

The most important pathogen for apples is Penicillium expansum that is the causal organism of blue mould. Postharvest losses are controlled with chemical fungicides such as TBZ but a growing concern for human health and a greater awareness for environmental conservation have multiplied the studies on new ecological technologies. In the search of ne...

Natural resistance against Venturia pirina and Botrytis cinerea was investigated within the Sardinian pears germplasm. The natural occurrence of V. pirina was monitored at the orchard level for 5 years, while resistance against B. cinerea was evaluated by a bioassay using methanolic extracts of the fruit rind or by artificially inoculation. Methano...

The International trade has to ensure food security and preserve both human health and the diffusion of animal and plant diseases among different countries. While organic horticultural production and trade are regulated by global guidelines, no specific restrictions relay to conventional commodities and almost free import-export occurs among UE cou...

The most important pathogen for table grapes is Botrytis cinerea which causes a rapid deterioration of fruit. Postharvest losses are controlled with SO2 fumigations carried out every 7 or 10 days, but the use of this gas is becoming more difficult to justify because of undesirable effects on the fruit and the increasing concern for human health. Ac...

Ultrasonic technology is known for many years and is used for several purposes such as sonochemistry, extraction of natural compounds, degassing of solvent, inactivation of enzymes and microorganism. In postharvest ultrasound is applied in not destructive analysis of crop for the determination of the maturation stage. Until now, however, the potent...

In the latest years, investigation on postharvest treatments has been increasingly addressed to preserve human health and environment safeguard. Several preservative compounds, physical treatments and biological control agents to restrain postharvest pathogens on horticultural products have been widely studied. Among them potassium sorbate (KS) has...

To gain knowledge on the influence of postharvest treatments with ultraviolet-C light upon the keeping quality of table grapes, a trail was performed employing commercially mature 'Corina', 'Dawn Seedless', 'Centennial Seedless' and 'Gran Perlon' grape cultivars (cvs). After grading, bunches were subjected to 0.0, 0.5, 1.5 or 3 kJm(-2) and then sto...

Penicillium expansum and Botrytis cinerea are among the pathogens most frequently affecting apples and grapes after harvest, respectively. We studied the behaviour of these moulds when subjected to different concentrations of methanol (MeOH) and dimethyl sulfoxide (DMSO) as a alternative method to fungicides in controlling postharvest decay of hort...

The restricted number of postharvest fungicides used in packing houses is leading to the selection of resistant strains of Penicillium digitatum (citrus green mould), one of the most common and serious pathogens during storage and marketing of lemons. Furthermore a growing concern for human health and a greater awareness for environmental conservat...

The employment of biocontrol agents to restrain postharvest pathogens is an encouraging approach, although, efficacy and consistency are still below those of synthetic pesticides. Up to date, the 'integrated control strategy' seems to be the most promising way to overcome this gap. Here, we report the feasibility to control postharvest decay caused...

The enduring confrontation between fungal pathogens and hosts during storage and shelf-life, along with the restricted number of fungicides for postharvest use, have led to the selection of biotypes able to overcome the mode of action of several fungicides, in several citrus growing areas. In order to control R-biotypes and fulfill the restrictions...

As a non destructive analysis method, Nuclear Magnetic Resonance Imaging (MRI) may provide helpful information on water movement and physiological effects of transpiration in senescing tissues. In the past decade, the use of MRI has provided a better understanding of some physiological disorders in horticultural product (e.g., internal breakdown) a...

In order to avoid rind damage following postharvest UV-C light treatment on sensible Citrus cultivars the illumination was performed after heat conditioning (HT). Experiments were performed with lemons (Citrus limon 'di Massa') harvested twice (April and June), graded and divided into 6 sets (each of 180 fruit) according to HT duration (hours) as f...

The keeping quality of three early ripening pear (Pyrus communis) cultivars, two belonging to the local biodiversity ('San Domenico' and 'San Giovanni Migliorata') and a National one ('Coscia') was evaluated, during the postharvest life, as affected by two hydrocooling temperatures. Fruit of 'S. Domenico' and 'S. Giovanni Migliorata' were harvested...

Imazalil (IMZ) was quantified in the flavedo and albedo (Citrus fruits outer and inner tissue of the exocarp) of wounded and unwounded Valencia L. Olinda oranges following a 2 min immersion at 25 degrees C in 50, 100, or 250 microg mL(-1) of the fungicide mixture with or without 3% sodium bicarbonate (SBC). The addition of SBC significantly reduced...

The activity of 2-deoxy-D-gLucose (2-DG) alone or in combination with a biocontrol yeast (Candida saitoana, strain 8C) was evaluated in vitro and in vivo against citrus green mould (Penicillium digitatum Sacc.). The in vitro assays were performed on amended potato dextrose agar (PDA) containing 0, 1.5, 3.0, 6.0, 15.0, 30.0 or 60.0 mM of 2-DG. P. di...

The behaviour of Penicillium digitatum and P. italicum was investigated when subjected to different concentrations of methanol (MeOH) and dimethyl sulfoxide (DMSO). The experiments were performed in 9 cm Petri dishes containing PDA amended with 0, 5, 10, 20, 30, 40 or 50 microL/mL of each of the single or combined chemicals. Daily, the formed colon...

'Coscia' is an early ripening pear with a short postharvest Life (1 month) and chemical treatments to prevent decay are generally not undertaken. This, along with the fast deterioration under shelf-life conditions, makes it difficult to contain postharvest moulds. 1-methylcyclopropene (1-MCP) has been employed with success to delay ripening and, as...

'Miali' and 'Caddina' are apple varieties of Sardinian germplasm, mainly produced under sustainable conditions. Fruit is rarely subjected to cold storage and postharvest losses are generally high. In order to prolong the marketing period and contain postharvest decay of these local varieties, we investigated on their storage behaviour and on the ef...

Storage behaviour of 'Core', 'Core Columbu', 'Fradis' and 'Meloni' white prunes, and a black one ('Sighera') of Sardinian germplasm were evaluated following immersion for 0 (control), 15, 30, 45 or 60 sec in water at 20, 50, 55 or 60 degrees C with or without 2% (w/v) NaHCO3 (SBC). As international varieties, fruit from one white plum ('Shiro') and...

In recent years several alternatives to the chemical control of postharvest decay have been examined but satisfactory levels of control, with a single system, have not been achieved yet. In the present study the results of an integrated postharvest approach are reported. Early and late harvested hybrid mandarin fruit “Fremont” and “Fairchild”, inoc...

The influence of the immersion of lemon fruit in aqueous imazalil (IMZ) mixtures at 25 or 50 degrees C on the deposition and persistence of IMZ within the fruit rind and its effectiveness in controlling postharvest green mold, caused by Penicillium digitatum, was investigated. Concentrations of IMZ in the albedo tissue surrounding deep wounds in th...

The feasibility to restrain citrus green mould (Penicillium digitatum Sacc.) during storage, by means of induced natural resistance, was investigated following combined heat and ultravioLet light C (254 nm, UV-C) treatments. Heat treatment (HT) was performed by keeping Citrus fruits in a humid saturated room at 36 degrees C for 36 h while, the UV-C...

Among the alternatives to synthetic postharvest fungicides encouraging results have been reported with biocontrol agents, and on Citrus fruits, their efficacy was improved when co-applied with GRAS compounds or with physical means. Still, the reason for this increased efficacy has not been explained and therefore a study was performed using orange...

In order to improve the treatment efficiency of conventional synthetic fungicides we investigated the relationship between the decay control efficacy and the concentration of imazalil (IMZ) when the fungicide was co-applied with NaHCO3 (SBC) for 2 min. Experiments were carried out using lemon fruit (Citrus limon (L.) Burm .f. cv Verna), either woun...

Citrus fruit are susceptible to many postharvest diseases and disorders, but Penicillium digitatum and Penicillium italicum are the most common and serious pathogens during storage and marketing. The continuous employ in packing houses of synthetic fungicides such as imazalil (IMZ) or thiabendazote for the control of these pathogens is promoting th...

The most important postharvest pathogen for table grape is Botrytis cinerea (gray mold), which cause a rapid deterioration of fruit. An effective control of the disease during storage is difficult and remains an unsolved problem since no pesticide treatments are allowed by European legislation. GRAS compounds, employed with no restriction as preser...

A diffusion model based on Fick's second law was used to simulate water transport in pear fruit at various conditions (20 °C and 75% RH; 1 °C and 60% RH). The finite element method was used to discretise the governing differential equations over the actual 3D pear geometry. For the first time, water transport in Conference pear fruits was described...

To identify early 'sentinel' molecules involved in the host-pathogen interaction between apricot plants and European Stone Fruit Yellows (ESFY) phytoplasma, three experiments have been performed. Eighteen trees, of eight apricot varieties grafted on Myrobalan, and maintained under insect-proof environment were patch-grafted four years ago with tiss...

The biocontrol yeast Rhodotorula glutinis, isolate 21A, obtained from tomato fruit was used to control Penicillium digitatum, P. italicum and Botrytis cinerea on artificially wounded citrus fruit. Orange and satsuma mandarin fruit were treated with the biocontrol yeast, inoculated with the pathogens and stored for 7 days at 23 degrees C. On orange...

Treatments as hot water dips or high temperature conditioning have been proven to be effective to control postharvest decay on various horticultural crops. These treatments reduce chilling injury and rot losses without causing detrimental effects on fruit firmness, flavour, taste or peel appearance. These technologies, aimed to control postharvest...

The toxic activity of 2-deoxy-D-glucose (2-DG) alone or combined with the biocontrol yeast Candida saitoana strain 8C was evaluated in vitro and in vivo against the postharvest fungal pathogen Penicillium digitatum. In order to assess the effect of the 2-DG on both the biocontrol yeast and fungal pathogen, in vitro tests were performed in Petri dis...

The combination of the yeast Candida oleophila strain '13L' with ultraviolet-C irradiation evidenced a synergistic effect in reducing P. digitatum mould and only 11% of the artificially inoculated wounds were infected. Adversely, when the bacteria Bacillus subtilis strain 'B160' was combined with ultraviolet-C irradiation no synergistic effect was...

Combined heat and UV treatments reduced decay and maintained quality of kumquat and 'Washington Navel' orange better than either treatment alone. Heat treatment followed by UV-C illumination was the most effective combination in controlling postharvest decay with hot water dip or hot air curing reducing the damage induced by subsequent UV-C treatme...

Following sodium carbonate treatment, accumulation of scoparone (6,7-dimethoxycoumarin) but not scopoletin (6-methoxy-7-hydroxycoumarin) was found in the albedo of wounded fruit from different Citrus sp. and cultivars. Treating wounded mandarin fruit cv. Fairchild with 5% Na(2)CO(3) (SC) lead to a scoparone accumulation in the albedo of 310, 361, a...

The trade of fresh fig fruit is restricted by its high perishability and numerous attempts have been done to extend the postharvest life. The main difficulties can be found in the fast ripening and the easiness of pathogen spread. Although the ripening can be slowed by low storage temperatures (close to 0 degrees C) the control of pathogens remains...

Generally recognised as save compounds (G.R.A.S) are attractive substitutes to synthetic chemicals in postharvest control diseases. They meet safety requirements, are cheap and able to be integrated with other disease control technologies. Among G.R.A.S compounds, carbonic acid salts have been investigated on carrots, bell pepper, melons, sweet che...

In order to work out a quarantine treatment for cactus pear fruit a factorial experimental plan was carried combining postharvest water dips at 20, 50, 54, 58 and 60 degrees C and storage at 1 degrees C for 3, 6 and 10 days. Cactus pear fruit cv 'Rossa' were artificially infested with Med. fly eggs (at least 20 eggs per fruit) then left in the lab...

Polygalacturonase inhibiting protein (PGIP) was extracted from 'Oroblanco' grapefruit type (triploid pummelo-grapefruit) albedo tissue, purified and partially characterized. Extraction was carried out at 4 degrees C with a high ionic strength extraction buffer. After dialysis and concentration by ultrafiltration the extract was chromatographed on c...