Guopeng MiaoHuainan Normal University · Department of Bioengineering
Guopeng Miao
Doctor of Philosophy
About
24
Publications
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Introduction
Transportation of secondary metabolites is extremely important for industry production using live organism. Because the endurance of cell has limits.
Additional affiliations
July 2011 - December 2014
March 2010 - December 2014
Education
May 2011 - October 2014
September 2009 - May 2011
September 2006 - May 2009
Publications
Publications (24)
Diverse secondary metabolites in plants, with their rich biological activities, have long been important sources for human medicine, food additives, pesticides, etc. However, the large-scale cultivation of host plants consumes land resources and is susceptible to pest and disease problems. Additionally, the multi-step and demanding nature of chemic...
Plant transporters regulating the distribution of secondary metabolites play critical roles in defending against pathogens, insects, and interacting with beneficial microbes. The phosphorylation of these transporters can alter their activity, stability, and intracellular protein trafficking. However, the regulatory mechanism underlying this modific...
BACKGROUND
As a type of biological control agents (BCAs), Bacillus velezensis possesses the efficacy of inhibiting pathogenic microorganisms, promoting plant growth, and overcoming continuous cropping obstacles (CCOs). However, there is limited reporting on the optimization of the cultivation conditions for such biocontrol agents and their role as...
As a safe and natural “capsule,” plants have several advantages over mammals and microorganisms for the production of oral vaccines. In this study, we innovatively utilized the ransmembrane region of the pea Translocase of chloroplast 34 (TOC34) protein to display two subunit vaccines, capsid protein VP2 of Porcine
parvovirus (PPV) and the heat-la...
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As a host for recombinant protein expression, plants have their own advantages over microorganisms and animal cells. When plant cells are used to express recombinant proteins, the extracellular secretion of recombinant proteins can simplify the purification process thereby reducing production costs. In view of the low efficiency of secretion mediat...
Chaetomium globosum can inhibit the growth of fusarium by means of their extracellular proteins. Two novel β-glucanases, designated Cgglu17A and Cgglu16B, were separated from the supernatant of C. globosum W7 and verified to have the ability to hydrolyze cell walls of Fusarium sporotrichioides MLS-19. Cgglu17A (397 amino acids) was classified as gl...
Key Message
TwPDR1, a PDR transporter from Tripterygium wilfordii Hook.f., was proved to efflux triptolide and its stability could be enhanced by A¹⁰³³T mutation.
Abstract
Triptolide, an abietane-type diterpene in Tripterygium wilfordii Hook.f., possesses many pharmacological activities. However, triptolide is in short supply and very expensive be...
BACKGROUND
Nanoparticles can improve the bioavailability of bioactive compounds. Concomitant intake of food can affect pharmacokinetic profiles by altering dissolution, absorption, metabolism, and elimination behavior. Studies on the effects of food and its supplements on the bioavailability of bioactives in nanoformulations are few. In this study,...
Pachymic acid (PA), a triterpenoid from Poria cocos, has various pharmacological effects, including anti-inflammatory, anti-cancer, anti-aging, and insulin-like properties. PA has gained considerable research attention, but the mechanism of its anti-cancer effects remains unclear. In this study, pyruvate kinase M2 (PKM2) was discovered as a PA targ...
Post-harvest disease is a major factor in the limited shelf life of many fruits and vegetables, and it is often managed by using fungicidal spraying or soaking. In this study, we first tested the efficiency of six common fungicides on post-harvest head cabbage (Brassica oleracea var. capitata) against Botrytis cinerea. Afterward, the elimination ab...
Cadmium is a ubiquitous environmental toxicant. The use of Caenorhabditis elegans as a model for monitoring cadmium exposure has revealed several conserved signaling pathways. However, little is known about the killing process during lethality assay. In the present study, we investigated the effects serotonergic neuronal and reproductive damages on...
Biological control of plant disease with plant growth-promoting rhizobacteria (PGPR) has increasingly become noteworthy as they provide an alternative and supplement to synthetic chemicals without environmental contamination. Numerous Bacillus species have been used for seed treatment, induction of systemic resistance and suppression of both root a...
Colonization of plants by particular endophytic fungi can provide plants with improved defenses toward pests and pathogens. In this study, an endophytic fungi, strain HS-1, was isolated from the root of Cucumis melo L. and characterized as Penicillium brefeldianum. The crude metabolites showed great antifungal and antinematodal activities in vitro...
Key message:
TwMDR1 transports sesquiterpene pyridine alkaloids, wilforine and wilforgine, into the hairy roots of T. wilfordii Hook.f. resulting in low secretion ratio of alkaloids. Hairy roots (HRs) exhibit high growth rate and biochemical and genetic stability. However, varying secondary metabolites in HR liquid cultures mainly remain in root t...
A filamentous actinomycete, designated strain ZX01(T), was isolated from forest soil around Kanas Lake of China. A polyphasic taxonomic study was carried out to establish the status of strain ZX01(T). Chemical and morphological properties of the isolate were similar to those of species of the genus Streptomyces. Analysis of the almost complete 16S...
The endophytic actinomycete F4-20 was isolated from Tripterygium wilfordii Hook.f. and was confirmed to produce wilforgine, a secondary metabolite discovered in its host. F4-20 showed a close phylogenetic relationship to Streptomyces species. To seek elicitors that may enhance the production of wilforgine in F4-20, four plant stress molecules were...
Tripterygium wilfordii Hook.f. (Celastraceae) produces a vast array of natural products, mainly terpenes and sesquiterpene pyridine alkaloids, which have strong biological activities. The limited gene information on the biosynthesis pathways restricts the metabolic engineering of this valuable natural resource. In this study, a suppression subtract...
Online Resource 1 Schematic diagram of the modified bubble column bioreactor; a air compressor, b air reservoir, c airfilter system, d air flow meter, e sparger, f inoculation container, g pH sensor, h DO sensor, i medium feeding port, j antifoaming agents port, k pH adjust ports, l medium (with XAD-7) port, m agitator, n ARs transfer port. Part of...
In order to solve the shortage of natural Tripterygium wilfordii Hook. f. plant resource for the production of important secondary metabolites triptolide and wilforine, hairy roots were induced from its root calli by Agrobacterium rhizogenes. Induced hairy roots not only could be maintained and grown well in hormone free half-strength Murashige and...
The experiments of elicitation and in situ adsorption were conducted in shake flasks and then tested in a modified bubble column bioreactor for enhancing the productions of three active metabolites in Tripterygium wilfordii Hook. f., triptolide, wilforgine and wilforine. Methyl jasmonate was screened out as the elicitor and the non-ionic polymeric...
The relationships between aggregate cell types, cell growth, and the triptolide, wilforgine, and wilforine content in aggregate cell suspension cultures of Tripterygium wilfordii Hook. f. were examined. Aggregate cells larger than 2 mm grew quickly and constituted the majority of the white aggregates. The accumulation of triptolide was strongly cor...
Questions
Questions (8)
I am now doing EMSA with biotin linked probe, however, I can't see any unbonded free nucleotide probe. Is this normal? Should I shorten the time of electrophoresis？
I am cloning a transporter gene of about 4.5 kb long. PCR was successful, however, when I tried to clone it into traditianal cloning vectors, such as pUC series, the target cDNA always rearranged into about 3 kb. There are tandem domains in this gene. So I wonder this may be the problem. Now, what should I do? I already tried different E. coli strains, but the problem still exists.
Is there any other vectors suitable for this unique cDNA? Is pJAZZ will do? Or, are there any other cloning systems instead of E. coli, such as yeast? Please help me on this, thank you all!
After adding 50% saturation of ammonium sulphate into a spent potato-dextrose liquid medium (300 mL), about 300 mg white precipitate showed up. Initially, I thought they should be proteins or lipopeptides, however, they don't have any UV absorption. I tested the solubility and found that only strong base (1 M NaOH) could dissolve them. They could be partially dissolved in 1 M HCl and not in methanol, acetone, or chloroform and ethyl acetate. How should I detect what're they? And what technique should be used for purification?
I am intended to make a kind of solid lipid nanoparticles to trap a lipophilic drug. I used glycerol tristearate 500 mg, oleinic acid 500 mg as emulsion compounds. These compouds were disovled in about 3 mL methane:chloroform (2:1) with 5 mg drug. The solution was heated to 70℃ and combined with 2.5% Pluronic F68 which was also preheated to 70℃. The emulsion procesure was done only by sonication for 10 min and then dropwise adding into 200 mL cold water.
The resulting suspension was ultrafiltrated and the filtrate was examined for the contents of the drug. The result showed that 80% of the drug was in the filtrate!! What's the problem here? Please help me.
Would somebody answer me that if the market sold Agrobacterium rhizogenes strains contain helper plasmid or not? Thank you.
During our study, we found that the expression of genes participating in the biosynthesis of plant alkaloids (in our study, sesquiterpene pyridine alkaloids) decreased after short time of MeJA treatment (around 6 to 9 h) showing a increase-decrease-increase pattern. This phenomena was also reported by another group (see reference: Camptothecin biosynthetic genes in hairy roots of Ophiorrhiza pumila: cloning, characterization and differential expression in tissues and by stress compounds). Why? We speculated that the lower expression level was caused by feedback inhibition of alkaloids influx from the culture medium. The feedback inhibition was also observed in other secondary metabolite biosynthesis. But what's the evolutionary sense of this action by plants?
