Biochemical Profile and Potential Medicinal Use of Ipomea Biloba Present in the Western Coastal Line of Arabian Sea at South India

Abstract

Ipomea biloba is an aquatic perennial runner plant used as a medical herb for various diseases. The plant were collected and extracted for biochemical compound isolation. In this present study the medicinal uses and biochemical parameters such as enzymes, amino acids, antioxidants, chlorophyll content, calcium, iron and total phenol present in the plant by using different methods

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International Journal of Research in Engineering and Bioscience ISSN: 2321-743X
Special Issue: National Conference on “Drug discovery, Threatened medicinal plants
and strategies for sustainable use” EDITED BY, S. DOMINIC RAJKUMAR & J. K. LAL
St. Andrew’s college, Gorakhpur, U.P., India
BIOCHEMICAL PROFILE AND POTENTIAL MEDICINAL USE OF IPOMEA BILOBA PRESENT IN THE WESTERN
COASTAL LINE OF ARABIAN SEA AT SOUTH INDIA - S.T.Gopukumar, V.N.Ariharan, and S.T.Sharmila
2013 Vol. 1 (Special Issue1) Page 136
BIOCHEMICAL PROFILE AND POTENTIAL MEDICINAL USE OF
IPOMEA BILOBA PRESENT IN THE WESTERN COASTAL LINE OF
ARABIAN SEA AT SOUTH INDIA
S.T.Gopukumar1*, V.N.Ariharan2, and S.T.Sharmila3
1Department of Nanotechnology, Noorul Islam University, Kumaracoil, India
2Department of Biomedical Engineering, Noorul Islam University, Kumaracoil, India
3Department of Biochemistry, M.G.R Arts and Science College, Hosur, India
Corresponding author E-mail id: stgopukumar@rediffmail.com
ABSTRACT
Ipomea biloba is an aquatic perennial runner plant used as a medical herb
for various diseases. The plant were collected and extracted for biochemical
compound isolation. In this present study the medicinal uses and biochemical
parameters such as enzymes, amino acids, antioxidants, chlorophyll content,
calcium, iron and total phenol present in the plant by using different methods.
Keywords: Ipomea biloba, amino acids, sap, erosion, enzymes, minerals

International Journal of Research in Engineering and Bioscience ISSN: 2321-743X
Special Issue: National Conference on “Drug discovery, Threatened medicinal plants
and strategies for sustainable use” EDITED BY, S. DOMINIC RAJKUMAR & J. K. LAL
St. Andrew’s college, Gorakhpur, U.P., India
BIOCHEMICAL PROFILE AND POTENTIAL MEDICINAL USE OF IPOMEA BILOBA PRESENT IN THE WESTERN
COASTAL LINE OF ARABIAN SEA AT SOUTH INDIA - S.T.Gopukumar, V.N.Ariharan, and S.T.Sharmila
2013 Vol. 1 (Special Issue1) Page 137
INTRODUCTION
In India, large diversity of plant
species is available; of these species some
are either beneficial or harmful. Fruits and
vegetables generally form indispensable
constituents of human diets supplying the
body with minerals, vitamins and certain
hormone precursors, in addition to small
amounts of protein and energy. The plant
Ipomea biloba belongs to the plant family
Convoluvulacea, a moving glory family. It
is a semi-aquatic tropical plant. In Tamil it
was known as “Kadamba Valli”. I.biloba
was mainly located on the west coast of
India, bounded by Arabian Sea. It is
established worldwide on many tropical
beaches including those of Australia and
the Caribbean [1][2]. The predominant soils
in the district are laetrile soils. The plant
size may be up to 100 feet long.
It grows well on nutrient-poor,
moist, sandy, or calcareous soils [3]. The
growth of I.biloba was extensive on the
sand dunes near the shore “cyanodon
dactylon” a gramiane member was also
found growing luxuriously along I.biloba.
This type of plant species are normally
seen under the coastal areas of Arabian
Sea. The leaves of the plants are greenish
in color [4]. The flower of I.biloba is pink
in nature. The plant produces adventitious
roots at the nodes and run horizontally
rather than vertically [5]. It does not tolerate
prolonged frost conditions. It provides
habitat for many diverse animal species
including gopher tortoise, the endangered
beach mice, scrub jay and the threatened
kestrel.
MATERIALS AND METHODS
I.biloba was collected from west
costal line of Manavalakurichi village,

International Journal of Research in Engineering and Bioscience ISSN: 2321-743X
Special Issue: National Conference on “Drug discovery, Threatened medicinal plants
and strategies for sustainable use” EDITED BY, S. DOMINIC RAJKUMAR & J. K. LAL
St. Andrew’s college, Gorakhpur, U.P., India
BIOCHEMICAL PROFILE AND POTENTIAL MEDICINAL USE OF IPOMEA BILOBA PRESENT IN THE WESTERN
COASTAL LINE OF ARABIAN SEA AT SOUTH INDIA - S.T.Gopukumar, V.N.Ariharan, and S.T.Sharmila
2013 Vol. 1 (Special Issue1) Page 138
Kanyakumari district, Tamilnadu, India.
The leaves were separated and cleaned
well. Cleaned leaves were then dried under
shade. The drying process was continued
until all the water molecules evaporated
and leaves became well dried for grinding.
The leaves were finely powdered and
extracted with acetone solvent using
Soxhlet apparatus.
In this study the presence of
Carbohydrates and Pentose was analyzed
by using Molisch test and Bial’s test
method. The presence of Tryptophan and
Histidine were analyzed by using Hopkins
cole and Pauly’s method. The estimation
of Protein was done by Biuret method.
Total Phenol was estimated by using Bray
and Thorpe method. The minerals such as
iron and calcium were estimated using
Clark & Coltip method and Dipyridyl
method. The enzymes amylase and
catalase were estimated by using Caraway
and Sodium Perborate method. The
Estimation of Lipid was done by using
Bilgh’s & Dyes method.
RESULTS
I.biloba is a plant with an immense
medicinal property in it. The plant plays a
vital role for both human as well as
environment. The sap from the succulent
leaves has been used as a first-aid to treat
jelly fish stings. Both leaves and stems
exude a watery white sap that may be a
chemical protection against insect pests
and grazing animals. The plant also serves
nature by preventing soil erosion. The
plant also provides Food & Shelter for
some sea insects and animals. The
qualitative and quantitative screeing of the
I.biloba were carried out and the results
were tabulated in table 1 and table 2
respectively.
Table: 1 Qualitative biochemical
screening of I.biloba
S.No
Compounds
Inference
1
Carbohydrates
+
2
Pentose
+
3
Tryptophan
+
4
Histidine
+
Table: 2 Quantitative biochemical
screening of I.biloba
Compounds
Contents
1
Protein (0.2ml)
0.357g/dl
2
Protein (0.5ml)
0.4286g/dl
3
Total Phenol
920µg/dl
4
Iron
320µg/dl
5
Calcium
10mg/dl
6
Amylase
137 caraways
7
Catalase
4.329×10-4
µmol/min
8
Lipid
82.39g
REFERENCES
1. Barnett, Michael R., and David
Crewz, editors. 1997. Common
Coastal Plants in Florida: A Guide

International Journal of Research in Engineering and Bioscience ISSN: 2321-743X
Special Issue: National Conference on “Drug discovery, Threatened medicinal plants
and strategies for sustainable use” EDITED BY, S. DOMINIC RAJKUMAR & J. K. LAL
St. Andrew’s college, Gorakhpur, U.P., India
BIOCHEMICAL PROFILE AND POTENTIAL MEDICINAL USE OF IPOMEA BILOBA PRESENT IN THE WESTERN
COASTAL LINE OF ARABIAN SEA AT SOUTH INDIA - S.T.Gopukumar, V.N.Ariharan, and S.T.Sharmila
2013 Vol. 1 (Special Issue1) Page 139
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