Gina Stella Garcia-Romo

Universidad Nacional Autónoma de México FES-Iztcala

Background Tumor-infiltrating lymphocytes are an important component of the tumor microenvironment (TME) in breast cancer. They have been linked with tumor pathogenesis in advanced stages. However, little is known about their contribution in early phases. In this study, we analyzed the infiltration of leukocytes and cancer stem cells (CSC) in tumor...

Plasmacytoid dendritic cells (pDCs) are antigen presenting cells specialized in viral recognition through Toll-like receptor (TLR)7 and TLR9, and produce vast amounts of interferon alpha upon ligation of these TLRs. We had previously demonstrated a strong influx of pDCs in the tubulointerstitium of renal biopsies at the time of acute rejection. How...

Even after the improvements made in recent years in early diagnosis and treatments, breast cancer is still the most common cancer and the leading cause of cancer death in women around the world. Several attempts to design new alternative therapies like immunotherapy have been evaluated in clinical trials, but they have shown limited efficacy. The f...

Even after the improvements made in recent years in early diagnosis and treatments, breast cancer is still the most common cancer and the leading cause of cancer death in women around the world. Several attempts to design new alternative therapies like immunotherapy have been evaluated in clinical trials, but they have shown limited efficacy. The f...

Even after the improvements made in recent years in early diagnosis and treatments, breast cancer is still the most common cancer and the leading cause of cancer death in women around the world. Several attempts to design new alternative therapies like immunotherapy have been evaluated in clinical trials, but they have shown limited efficacy. The f...

Neutrophils are one the earliest, crucial innate defenses against innumerable pathogens. Their main microbicidal activities include phagocytosis and degranulation, with many pharmacologically active molecules contributing to inflammation. Recently, a novel antimicrobial mechanism was discovered; the Neutrophil Extracelullar Traps (NETs) formed by e...

CD4+ type 1 T regulatory (Tr1) cells have a crucial role in inducing tolerance. Immune regulation by these cells is mainly mediated through the secretion of high amounts of IL-10. Several studies have suggested that this regulatory population may be involved in tumor-mediated immune-suppression. However, direct evidence of a role for Tr1 cells in h...

Currently Staphylococcus aureus is the predominant pathogen isolated from the respiratory tract of patients with recurrent tonsillitis (RT). Because of the rise in multi-drug resistant strains of S. aureus, there is a pressing need for effective treatments and preventive approaches that can avoid the risk of invasive and life-threatening infections...

Analysis of mitochondrial membrane integrity from Mfs and DCs exposed either to PMA alone, or to NETs at two different ratios. Percentage of macrophages (A-D) or dendritic cells (E-H) incubated with medium alone (A,E), 100 nM PMA (B,F), APC 1:1 NETs (C,G) and APC 1:2 NETs (D,H) for 3 h. Horizontal bars in the histograms indicate the region with nor...

Neutrophil extracellular traps (NETs). Unstimulated (A-D) and PMA-stimulated (E-H) blood neutrophils were labeled for DNA (Blue), Elastase (Red) and Histone (Green). DNA staining (DAPI) is shown in (C,G). Immunostaining of neutrophil elastase is shown in (B,F), histone in (A,E), and the merge in (D,H). Stimulated PMNs were washed twice with culture...

Alveolar macrophages (AM) and dendritic cells (DCs) are the main antigen presenting cells (APCs) in the respiratory tract. Whereas macrophages have been extensively studied in tuberculosis, in situ interactions of DC with Mycobacterium tuberculosis (Mtb) are poorly explored. We aimed to characterize lung APCs during pulmonary tuberculosis in Balb/C...

Systemic lupus erythematosus (SLE) is a systemic autoimmune disease characterized by a breakdown of tolerance to nuclear antigens and the development of immune complexes. Genomic approaches have shown that human SLE leukocytes homogeneously express type I interferon (IFN)-induced and neutrophil-related transcripts. Increased production and/or bioav...

Despite tuberculosis resurgence and extensive dendritic cell (DC) research, there are no in vivo studies evaluating DC within regional lymphoid tissue during airways infection with virulent Mycobacterium tuberculosis (Mtb) H37Rv. Using DC-specific antibodies, immunocytochemistry, flow cytometry and Ziehl-Neelsen (ZN) for bacilli staining, we search...

Scarce information exists about the role of lung antigen‐presenting cells (APCs) in vivo during pulmonary tuberculosis. As APCs activate cellular immunity, following intratracheal inoculation with virulent Mycobacterium tuberculosis , we assessed in situ lung APC recruitment, distribution, granuloma involvement, morphology and mycobacterial burden...

Experimental protocols for cancer immunotherapy include the utilization of autologous monocyte-derived dendritic cells (moDC) pulsed with tumor antigens. However, disease can alter the characteristics of monocyte precursors and some patients have increased numbers (up to 40%) of the minor CD16(+) monocyte subpopulation, which in healthy individuals...