Franck Merlier

Université de Technologie de Compiègne | UTC · Génie Enzymatique et Cellulaire (GEC) - UMR 7025

The liver is one of the main organs involved in the metabolism of xenobiotics and a key organ in toxicity studies. Prior to accessing the hepatocytes, xenobiotics pass through the hepatic sinusoid formed by liver sinusoidal endothelial cells (LSECs). The LSECs barrier regulates the kinetics and concentrations of the xenobiotics before their metabol...

Atrazine, the most commonly used herbicide, has been reported to pollute the water environment and do harm to human health. It is thus urgent to find an efficient way to degrade atrazine. Although various advanced oxidation processes including high voltage electrical discharge (HVED) have been applied to degrade atrazine, the formation kinetics of...

Animal models are considered prime study models for inhalation-like toxicity assessment. However, in light of animal experimentation reduction (3Rs), we developed and investigated an alternative in vitro method to study systemic-like responses to inhalation-like exposures. A coculture platform was established to emulate inter-organ crosstalks betwe...

Tumor Necrosis Factor‐α (TNF‐α) is a cytokine that is normally produced by immune cells when fighting an infection. But, when too much TNF‐α is produced as in autoimmune diseases, this leads to unwanted and persistent inflammation. Anti‐TNF‐α monoclonal antibodies have revolutionized the therapy of these disorders by blocking TNF‐α and preventing i...

Tumor Necrosis Factor‐α (TNF‐α) is a cytokine that is normally produced by immune cells when fighting an infection. But, when too much TNF‐α is produced as in autoimmune diseases, this leads to unwanted and persistent inflammation. Anti‐TNF‐α monoclonal antibodies have revolutionized the therapy of these disorders by blocking TNF‐α and preventing i...

Background and Aims Mitochondrial dysfunction and energy metabolism deficiency are part of the acute kidney injury (AKI) pathophysiology. In particular, recent studies highlighted the role of nicotinamide adenine dinucleotide (NAD) production in the pathogenesis of AKI. NAD is an electron carrier for mitochondria and a cofactor for cytoplasmic redo...

High-resolution mass spectrometry (HRMS) was coupled with ultra-high-performance liquid chromatography (UHPLC) to simultaneously quantify trehalose and trehalose 6-phosphate without derivatization or sample preparation. The use of full scan mode and exact mass analysis also makes it possible to carry out metabolomic analyses as well as semi-quantif...

Polyphosphate accumulating organisms (PAOs) are responsible for enhanced biological phosphate removal (EBPR) from wastewater, where they grow embedded in a matrix of extracellular polymeric substances (EPS). EPSs comprise a mixture of biopolymers like polysaccharides or (glyco)proteins. Despite previous studies, little is known about the dynamics o...

The liver is one of the main organs involved in the metabolism of xenobiotics and a key organ in toxicity studies. Prior to accessing the hepatocytes, xenobiotics pass through the hepatic sinusoid formed by liver sinusoidal endothelial cells (LSECs). The LSECs barrier regulates the kinetics and concentrations of the xenobiotics before their metabol...

High resolution mass spectrometry (HRMS) was coupled with ultra-high-performance liquid chromatography (uHPLC) to monitor atrazine (ATZ) degradation process of Fenton/ultra-sound (US) treatment in real time. Samples were automatically taken through a peristaltic pump, and then analysed by HPLC-HRMS. The injection in the mass spectrometer was perfor...

Polyphosphate accumulating organisms (PAOs) are responsible for enhanced biological phosphate removal (EBPR) from wastewater, where they grow embedded in a matrix of extracellular polymeric substances (EPS). EPS comprise a mixture of biopolymers like polysaccharides or (glyco)proteins. Despite previous studies, little is known about the dynamics of...

Atrazine, an herbicide used to control grassy and broadleaf weed, has become an essential part of agricultural crop protection tools. It is widely sprayed on corn, sorghum and sugar cane, with the attendant problems of its residues in agri-food and washing water. If ingested into humans, this residual atrazine can cause reproductive harm, developme...

Cadherins are cell-surface proteins that mediate cell-cell adhesion. By regulating their grip formation and strength, cadherins play a pivotal role during normal tissue morphogenesis and homeostasis of multicellular organisms. However, their dysfunction is associated with cell migration and proliferation, cancer progression and metastasis. The cons...

Introduction CKD is associated with alterations of tubular function. Renal gluconeogenesis is responsible for 40% of systemic gluconeogenesis during fasting, but how and why CKD affects this process and the repercussions of such regulation are unknown. Methods We used data on the renal gluconeogenic pathway from more than 200 renal biopsies perfor...

Molecularly imprinted polymer (MIP) nanoparticles-based differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS) chemosensors for antiplatelet drug substance, cilostazol (CIL), and its pharmacologically active primary metabolite, 3,4-dehydrocilostazol (dhCIL), selective determination in human plasma were devised, prepar...

MIPs for protein recognition are often synthesized by the epitope approach. The epitope is a short surface-exposed peptide of the protein. Solution STD and WaterLOGSY NMR spectroscopies were used to prove the evidence of specific binding sites created by the imprinting of the peptide epitope in the corresponding MIP nanogel. No binding was observed...

Molecularly imprinted polymers (MIPs) are tailor‐made synthetic antibodies possessing specific binding cavities designed for a target molecule. Currently, MIPs for protein targets are synthesized by imprinting a short surface‐exposed fragment of the protein, called epitope or antigenic determinant. However, finding the epitope par excellence that w...

A novel water-soluble polysaccharide named AGP1 was successfully isolated from seeds of Anethum graveolens by hot water extraction and further purified by DEAE-Sepharose chromatography. AGP1 has a relative molecular weight of 2.1 10⁴ Da determined by Ultra-high-performance liquid chromatography (UHPLC). The AGP1 characterization was investigated by...

New bio-based molecularly imprinted polymers (MIPs) were synthesized as biopesticide delivery systems by free radical polymerization using a functional monomer and a vegetable oil-derived cross-linker, epoxidized soybean oil acrylate (ESOA). Resveratrol, a polyphenol with antifungal activity against several phytopathogens, was employed in this stud...

The growing uses of functional polymers in different domains and the well-established remanence of synthetic polymers underline the necessity to study their microbial biodegradation. A phylloplane bacterial collection was screened for its potential to overcome documented factors limiting polymer biodegradation, i.e. low cleavable long chain linear...

RATIONALE While the GC-Orbitrap, marketed in 2015, represents a technological breakthrough in terms of sensitivity, resolution and mass stability, many studies have reported ion ratio modification in mass spectra using the standard 70 eV electron ionization. Methods: We studied herein the influence of the acquisition and sample parameters leading...

One of the most promising strategies to treat cancer is the use of therapeutic antibodies that disrupt cell–cell adhesion mediated by dysregulated cadherins. The principal site where cell–cell adhesion occurs encompasses Trp2 found at the N‐terminal region of the protein. Herein, we employed the naturally exposed highly conserved peptide Asp1‐Trp2‐...

One of the most promising strategies to treat cancer, entails the use of therapeutic antibodies which disrupt cell‐cell adhesion mediated by dysregulated cadherins. The principal site where cell‐cell adhesion occurs, encompasses a Trp2 found at the N‐terminal region of the protein. Herein, we employed the naturally exposed highly conserved peptide...

Molecularly imprinted polymers (MIPs) are synthetic materials with recognition properties comparable to those of antibodies, but with superior physico-chemical stability. Synthesized in presence of a template, MIPs contain binding sites with high specificity and selectivity for their target. In this work, we explored the radical-mediated thiol-yne...

Botrytis cinerea is a necrotrophic fungus that affects various plant species. Chemical control is a necessity and as much as possible, eco-friendly conditions and bioresources to obtain these chemicals should be used. In this context, a series of products was obtained from salicylaldehyde using zinc as a powerful reagent and tested for antifungal a...

We describe the preparation and characterization of synthetic antibodies based on molecularly imprinted polymer nanoparticles (MIP-NPs), for the recognition and binding of the highly conserved and specific peptide motif SWSNKS (3S), an epitope of the envelope glycoprotein 41 (gp41) of human immunodeficiency virus type 1 (HIV-1). This motif is impli...

Fatty acid methyl esters (FAMEs), which are commonly used to characterize lipids, have several limitations to conclude on many structures. 3-Pyridylcarbinol esters (3-PCE) are used to characterize fatty acid structures, in particular, to identify ring and double bond positions on the carbon chain. Chromatographic separation of these esters is compl...

In context of fluxomic studies, 13C labeling analysis of amino acids are very important for solving the carbon flow calculation, because they are synthesized in various biosynthesis pathways and cellular compartments in plant cells. Traditionally, 13C labeling analysis are performed using low resolution mass spectrometry detector by GC-MS. We compa...

We describe the synthesis of water-soluble molecularly imprinted polymer nanoparticles (MIP-NPs) as a new artificial host receptor for the recognition of adenosine monophosphate (AMP), used herein, as a model nucleotide. MIP-NPs were prepared by solid-phase synthesis on glass beads (GB) using, for the first time, immobilized Fe(III)-chelate as an a...

Xerophyta humilis is a poikilochlorophyllous monocot resurrection plant used as a model to study vegetative desiccation tolerance. Dehydration imposes tension and ultimate loss of integrity of membranes in desiccation sensitive species. We investigated the predominant molecular species of glycerolipids present in root and leaf tissues, using multip...

A microfluidic liver biochip was coupled with a mass spectrometer to detect in real time the drug metabolism of hepatocytes. The Hepatocytes were cultivated in the biochip for 35h hours. The biochip was located in a small-scale incubator in which temperature and CO2 were controlled. The biochip was connected serially to the mass spectrometer, a per...

Objective: In the present study, the essential oil of Athroisma proteiformis was characterized for the first time. Method: GC-HRMS (Gas Chromatography-High Resolution Mass Spectrometry) was performed for qualitative and quantitative analysis of the phytochemicals present in the extract. Chemical analyses were conducted to evaluate the total phenols...

The data presented in this article are related to the research article entitled “13C labeling analysis of sugars by high resolution-mass spectrometry for Metabolic Flux Analysis” (S. Acket, A. Degournay, F. Merlier, B. Thomasset, 2017) [1]. This article provides data concerning the comparison between the theoretically expected values of free sugars...

Suite à la demande croissante en huiles utilisées dans différents domaines (agroalimentaire, cosmétique, biocarburants…), la recherche de nouvelles sources d’acides gras semble incontournable. Bien que certaines plantes soient largement utilisées pour l’extraction d’huiles à intérêt industriel comme la palme et le colza, la diversité végétale reste...

Metabolic flux analysis is particularly complex in plant cells because of highly compartmented metabolism. Analysis of free sugars is interesting because it provides data to define fluxes around hexose, pentose, and triose phosphate pools in different compartment. In this work, we present a method to analyze the isotopomer distribution of free suga...

Objective In the present study, the essential oil of Athroisma proteiformis was characterized for the first time. Method GC-HRMS (gas chromatography-high resolution mass spectrometry) was performed for qualitative and quantitative analysis of the phytochemicals present in the extract. Chemical analyses were conducted to evaluate the total phenols,...

Betanin is a natural pigment with significant antioxidant and biological activities currently used as food colorant. The isolation of betanin is problematic due to its instability. In this work, we developed a fast and economic procedure based on molecularly imprinted solid-phase extraction (MISPE) for the selective clean-up of betanin and its ster...

Molecularly imprinted polymers (MIPs) are synthetic antibody mimics capable of specific molecular recognition. Advantageously, they are more stable, easy to tailor for a given application and less expensive than antibodies. These plastic antibodies are raising increasing interest and one relatively unexplored domain in which they could outplay thes...

We have cultivated, for 13 days, human primary hepatocytes in a microfluidic bioreactor and in Petri. mRNA kinetics have shown in biochips an increase of the levels of CYP2B6, CYP2C19, CYP2C8, CYP3A4, CYP1A2, CYP2D6, HNF4a, SULT1A1, UGT1A1 mRNA related genes when compared to post extraction levels. In addition, comparison with Petri have shown a hi...

Recent studies have pointed out the preventive role of beetroot extracts against cancers and their cytotoxic activity on cancer cells. Among many different natural compounds, these extracts contained betanin and its stereoisomer isobetanin, which belongs to the betalain group of highly bioavailable antioxidants. However, a precise identification of...

A new in vitro microfluidic platform(Integrated Insert Dynamic Microfluidic Platform, IIDMP) allowing the coculture of intestinal cells Caco-2 TC7 and of human primary hepatocytes was used to test the absorption and first pass metabolism of two drugs : phenacetin and omeprazole. The metabolism of these drugs by CYP1A2, CYP2C19 and CYP3A4 was evalua...

We developed a microfluidic platform to investigate paracetamol intestinal and liver first pass metabolism. This approach was coupled with a mathematical model to estimate intrinsic in vitro parameters and to predict in vivo processes. The kinetic modeling estimated the paracetamol and paracetamol sulfate permeabilities, the sulfate and glucuronide...

Carnitine is an essential quaternary ammonium amino acid that occurs in the microbial, plant and animal kingdoms. The role and synthesis of this compound are very well documented in bacteria, fungi and mammals. On the contrary, although the presence of carnitine in plant tissue has been reported four decades ago and information about its biological...

The development of an enzyme based biosensing method is described for evaluating the toxicity of solutions treated by titanium dioxide photocatalysis. The method is based on the potential of rat liver microsomal glutathione transferase ability (mGST) to get enhanced in the conditions of chemical and oxidative toxicity. Phenol is taken as a model po...

Experimental design and samples labelling of the proteomic analysis. Each biochip and Petri is the pool of three independent replicates. (DOC)

Differentially expressed proteins by the treatment effect in biochip and successfully identified by MS/MS. (DOC)

Differentially expressed genes by the APAP treatment in biochip when compared to the untreated biochip (fold change above 1.8 or below 0.55). (DOC)

Differentially expressed genes by the APAP treatment in Petri when compared to the untreated Petri (fold change above 1.8). (DOC)

Proteins differentially expressed by the biochip versus the Petri and successfully identified by MS/MS. (DOC)

Differentially expressed proteins by the treatment effect in Petri dishes and successfully identified by MS/MS. (DOC)

Microfluidic bioartificial organs allow the reproduction of in vivo-like properties such as cell culture in a 3D dynamical micro environment. In this work, we established a method and a protocol for performing a toxicogenomic analysis of HepG2/C3A cultivated in a microfluidic biochip. Transcriptomic and proteomic analyses have shown the induction o...

A molecularly imprinted polymer (MIP), templated with methyltestosterone, has been synthesized for the cleanup of hydrolyzed urine samples for subsequent testosterone (T) quantification by LC-MS/MS. A concentration of 2 ng/mL testosterone could be quantified after a single step extraction on the MIP. The limit of detection and quantification with t...

19th International Congress of Chemical and Process Engineering - CHISA 2010 & 7th European Congress of Chemical Engineering - ECCE-7, Prague, Czech Republic, 28 August - 1 September 2010.

The main aim of this paper is the evaluation of the electrochemical behavior of the WC-Co layers. In this paper, which lies within the scope of a general characterization of coatings WC-Co, a particular point is related to the electrochemical behavior in sea water of these deposits projected by APS and Jet-Kote in relation to their microstructure....