Estefan Miranda-Miranda

INIFAP Instituto Nacional de Investigaciones Forestales Agricolas y Pecuarias | INIFAP · Departamento de Sanidad Animal

Ticks and tick-borne diseases have gained increasing attention in recent years due to their impact on public health and significant losses in livestock production. The use of synthetic compounds for tick control is becoming problematic, mainly due to the resistance to commercially available products as well as their toxicity. Therefore, new alterna...

Ticks and tick-borne diseases have gained increasing attention in recent years due to their impact on public health and significant losses in livestock production. The use of synthetic compounds for tick control is becoming problematic, mainly due to the resistance to commercially available products as well as their toxicity. Therefore, new alterna...

Rhipicephalus microplus is a persistent ectoparasite of cattle that causes bovine anaplasmosis and babesiosis, causing economic losses worldwide. Chemical treatment is the primary method for tick control, but the emergence of pesticide-resistant ticks is a major challenge. Alternative biocontrol strategies utilizing entomopathogenic microorganisms...

In this study, we aimed to develop a comprehensive methodology for identifying amino acid polymorphisms in acetylcholinesterase transcript 2 (AChE2) in acaricide-resistant Rhipicephalus microplus ticks. This included assessing AChE2 expression levels through qPCR and conducting 3D modeling to evaluate the interaction between acaricides and AChE2 us...

The search for targets to control ticks and tick-borne diseases has been an ongoing problem, and so far, we still need efficient, non-chemical alternatives for this purpose. This search must consider new alternatives. For example genomics analysis is a widely applied tool in veterinary health studies to control pathogens. On the other hand, we prop...

The expression of the Fasciola hepatica carboxylesterase type B (CestB) gene is known to be induced upon exposure to the anthelmintic triclabendazole (TCBZ), leading to a substantial rise in enzyme-specific activity. Furthermore, the nucleotide sequence of the CestB gene displays variations that can potentially result in radical amino acid substitu...

The search for targets to control ticks and tick-borne diseases has been an ongoing problem, and so far, we still need efficient, non-chemical alternatives for this purpose. This search must consider new alternatives. For example genomics analysis is a widely applied tool in veterinary health studies to control pathogens. On the other hand, we prop...

The expression of the Fasciola hepatica Carboxylesterase type B (CestB) gene is induced in response to the anthelmintic triclabendazole (TCBZ), resulting in a significant increase in enzymatic specific activity. Furthermore, the amino acid sequence of CestB exhibits variations that could lead to substantial amino acid substitutions at the ligand-bi...

Aspergillus flavus has been found to be an effective entomopathogenic fungus for various arthropods, including ticks. In particular, natural fungal infections in cattle ticks show promise for biocontrol of the Rhipicephalus (Boophilus) microplus tick, which is a major ectoparasite affecting cattle worldwide. Our study aimed to elucidate the specifi...

Introducción: Las moscas sierra (Hym. Diprionidae) afectan las coníferas y otras especies forestales en el hemisferio norte, reduciendo la productividad del bosque y causando la muerte de los rodales, lo que justifica acciones de control.Objetivo: Obtener cepas baculovíricas de poblaciones de moscas sierra mexicanas y explorar su potencial para ela...

Fasciola hepatica anthelmintic resistance may be associated with the catalytic activity of xenobiotic metabolizing enzymes. The gene expression of one of these enzymes, identified as carboxylesterase B (CestB), was previously described as inducible in adult parasites under anthelmintic treatment and exhibited a single nucleotide polymorphism at pos...

Ticks are hematophagous ectoparasites with importance to animal and human health. In recent years, the study of ticks has had significant development, including immune response, vector-host interactions, physiological and multi-omics approaches. However, one of the main impediments is obtaining a significant amount of high-quality hemolymph. For th...

Ticks are hematophagous ectoparasites that infest a diverse number of vertebrate hosts. The tick immunobiology plays a significant role in establishing and transmitting many pathogens to their hosts. To control tick infestations, the acaricide application is a commonly used method with severe environmental consequences and the selection of tick-res...

Bioinformatics analysis of the complete transcriptome of Fasciola hepatica, identified a total of ten putative carboxylesterase transcripts, including a 3146 bp mRNA transcript coding a 2205 bp open reading frame that translates into a protein of 735 amino acids, resulting in a predicted protein mass of 83.5 kDa and a putative carboxylesterase B en...

We describe the selection, culture, maintenance and rearing of the cattle tick Rhipicephalus (Boophilus) microplus at CENAPA-SENASICA-SADER, Mexico, where reference susceptible and acaricide-resistant tick strains are reared and toxicologically tested. The purpose of maintaining these tick strains is to evaluate the efficacy of diverse acaricides p...

Fasciola hepatica is a worldwide distributed zoonotic parasitic trematode, which causes a severe liver disease clinically known as fasciolasis in a large number of wild animals, several livestock species as well as humans, prevention and control of fasciolasis is made by massive use of anthelmintic compounds on livestock and inevitably this practic...

Background: Acaricide resistance is a central problem for the control of the cattle tick Rhipicephalus microplus. The physiological effects and phenotypes of the mutations that cause acaricide resistance are not always well understood or characterized. Single nucleotide polymorphisms (SNPs) that confer cypermethrin knockdown resistance (kdr) have...

Background: Acaricide resistance is a central problem for the control of the cattle tick Rhipicephalus microplus. Genetic changes that confer resistance get fixed in the population, however, the physiological effects and phenotypes of these mutations are not always well understood or characterized. SNPs in the para-sodium channel gene that confer c...

Background: Acaricide resistance is a central problem for the control of the cattle tick Rhipicephalus microplus . Genetic changes that confer resistance get fixed in the population, however, the physiological effects and phenotypes of these mutations are not always well understood or characterized. SNPs in the para-sodium channel gene that confer...

Background: Acaricide resistance is a central problem for the control of the cattle tick Rhipicephalus microplus. Genetic changes that confer resistance get fixed in the population, however, the physiological effects and phenotypes of these mutations are not always well understood or characterized. Single Nucleotide Polymorphisms (SNPs) in the para...

RESUMEN. El artrópodo Rhipicephalus microplus, es un ectoparásito hematófago que representa un serio problema en la industria ganadera mundial, por su gran capacidad expoliativa y vectorial para transmitir organismos patógenos al ganado bovino, como Babesia spp. y Anaplasma spp., En este sentido, R. microplus, posee una amplia variedad de mecanismo...

La garrapata R. microplus, es la especie de mayor incidencia económica y sanitaria debido a su capacidad expoliativa y vectorial de transmisión de agentes infecciosos como Babesia bigemina, Babesia bovis y Anaplasma marginale. En este sentido, R. microplus, posee una amplia variedad de mecanismos inmunológicos de protección, incluyendo la producció...

Although genome sequencing has become a very promising approach to conduct microbial taxonomy, few labs have the resources to afford this especially when dealing with data sets of hundreds to thousands of isolates. The goal of this study was to identify the most adequate loci for inferring the phylogeny of the species within the genus Staphylococcu...

Although genome sequencing has become a very promising approach to conduct microbial taxonomy, few labs have the resources to afford this especially when dealing with data sets of hundreds to thousands of isolates. The goal of this study was to identify the most adequate loci for inferring the phylogeny of the species within the genus Staphylococcu...

Publicaly available genomes used for this study The 265 publicaly available genomes, from 46 species, were included, 40 of them are type strains (marked in bold). Genome statics were taken from NCBI. The third column gives the strain code used for figures 1, 2 and 4.The red bold row shows Staphylococcus saprophyticus UBA1057, which was removed from...

Functional enrichment analysis GO enrichment analysis via Blast2GO PRO.

Newly sequenced genomes for this study Genome assembly statistics of thenewly sequenced Staphylococci strains.

List of the 177 SGF without signals of recombination Complete list of the 177 SGF without signals of recombination, which are our orthologous groups. Protein accession numbers were taken from the S. aureus reference genome TW20, the corresponding gene name was checked in Uniprot.

Robinson-Foulds distances between all the SGF trees Histogram of the Robinson-Foulds distances estimated between all the SGF trees. There were no two identical topologies and on average topologies are 47% similar to each other.

Intra-species genetic diversity for S. aureus and S. epidermidis Histograms of the intra-species nucleotide diversity for S. aureus and S. epidermidis.

Neighbour Nets and recombination test for rpoB and tuf Phylogenetic networks built with SplitsTree, for two (rpoB and tuf) of the usual taxonomic markers. These markers are part of the 208 single copy genes but failed the recombination test. Strain labels are not shown for sake of clarity.

The goal of the present study was to assess the gene expression of xenobiotic metabolizing enzymes (XMEs) Cytochrome P-450 (CYP) and carboxylesterase (CE) related to detoxification of synthetic pyrethroids, plus acetylcholinesterase (AChE), in field isolates of acaricide-resistant Rhipicephalus microplus. The XMEs expression levels were assessed by...

The cattle tick Rhipicephalus (Boophilus) microplus is one of the most harmful ectoparasites affecting bovines worldwide. It represents a major threat to livestock industry due to the economic losses caused and diseases associated with these ticks. The most important tick control strategy has been the use of ixodicides, resulting in chemically resi...

Background: Fasciola hepatica is the main agent of fasciolosis, a zoonotic disease affecting livestock worldwide, and an emerging food-borne disease in humans. Even when effective treatments are available, drugs are costly and can result in tolerance, liver damage and normally they do not prevent reinfection. Drug-resistant strains in livestock ha...

Enterococcus spp. are Gram-positive lactic acid-producing bacteria found in the intestinal tracts of animals, like mammals, birds, and arthropods. Enterococcus spp. may cause oportunistic infections in vertebrate and invertebrate hosts. We report here the draft genome sequence of Enterococcus casseliflavus PAVET15 containing 3,722,480 bp, with 80 c...

A zymogram assay for Glutathion S Transferase (GST) was assessed, as an alternative diagnostics procedure for acaricide resistance detection in the cattle tick Rhipicephalus microplus using tick extracts from different developmental stages. GST is involved in the metabolic neutralization of toxins in a broad range of arthropods and associated with...

RESUMEN. Se colectaron garrapatas de Rhipicephalus (Boophilus) microplus durante los años 2010 a 2015 de bovinos infestados experimentalmente. Estas garrapatas mostraron signos de infección natural, como la presencia de exudado en el hipostoma y poro genital, cambio de coloración de la cutícula y bloqueo en la oviposición de hembras ingurgitadas qu...

RESUMEN. Como un procedimiento alternativo al diagnóstico de la resistencia de Rhipicephalus microplus a los acaricidas, este trabajo hace la evaluación de un ensayo de zimogramas de la enzima glutatión S transferasa (GST) a partir de extractos obtenidos de diferentes estadios de desarrollo de la garrapata del ganado. Para este ensayo, se determina...

The trematode Fasciola hepatica is the causative agent of a parasitic zoonosis known as fasciolosis, disease that affects humans and most species of domestic animals. Triclabendazole (TCBZ) is the most widely used fasciolicide, however, its indiscriminate use has led to the expression of anthelmintic resistance in the liver fluke. In the present wo...

The tyraminergic/octopaminergic system is central for the control of arthropod oviposition. Previous works demonstrated that the pharmacological perturbation of this system inhibits oviposition in the cattle tick Rhipicephalus microplus. In this work, we describe a physiologically active whole-mount preparation of the contractile tick ovary that al...

Paenibacillus larvae strain MEX14 is a facultative anaerobic endospore-forming bacterium that infects Apis mellifera larvae. Strain MEX14 was isolated from domestic bee larvae collected in a backyard in Mexico City. The estimated genome size was determined to be 4.18 Mb, and it harbors 4,806 protein coding genes (CDSs).

The aim of the present study was to genetically characterize Fasciola hepatica strains from diverse ecogeographical regions (America and Europe), susceptible and resistant to Triclabendazole, using the random amplified polymorphic DNA fragments (RAPDs-PCR) technique to elucidate genetic variability between the different isolates. Ten different olig...

A moderate halophile and thermotolerant fungal strain was isolated from a sugarcane bagasse fermentation in the presence of 2 M NaCl that was set in the laboratory. This strain was identified by polyphasic criteria as Aspergillus caesiellus. The fungus showed an optimal growth rate in media containing 1 M NaCl at 28°C and could grow in media added...

Rhipicephalus (Boophilus) microplus females do not deposit eggs during symptoms of genital infection. In experimental conditions 5% of engorged female ticks showed symptoms of bacterial infection. The symptoms are evident females secreted a distinctive yellow exudate through the genital orifice and eventually died. We obtained 62 bacterial isolates...

The objective of this work was to study and characterize the Rhipicephalus (Boophilus) microplus embryogenesis from freshly laid eggs to hatching larvae. Eggs were fixated every 24 hr. and treated eggs were placed on a microscope glass slide and stained with 4',6-diamidino-2-phenylindole (DAPI).Specimens were imaged on an epifluorescence microscope...

Steroid hormone-like molecules exert control on developmental changes of helminthes into fully reproductive adults. All invertebrates exhibit a very delicate balance of circulating steroids and the ubiquitous enzymes carboxylesterases are necessary for hydrolyzing excess hormone levels or completely depleting it for the final transition into adulth...

BACKGROUND: Fasciolasis is a zoonotic parasitic disease caused by Fasciola hepatica. Closantel is a flukicide and has been shown to have an efficacy in vivo e in vitro against mature and immature stages, does not exhibit ovicidal activity in vitro and the ovicidal effects in vivo at present are undetermined. Egg hatch assay (EHA) is a laboratory to...

The cattle tick Rhipicephalus microplus, is one of the most damaging livestock ectoparasites. Tropical tick infestation limits the introduction of high-yield bovine varieties because they do not have immunity to the diseases transmitted by these ectoparasites. This tick is usually controlled with chemical acaricides but their indiscriminate use has...

In the present work, we evaluate in vivo the activity of carboxylesterase of Fasciola hepatica exposed to triclabendazole. We observed a statistically significant increase in enzyme activity at 24 and 48h post treatment (P<0.01 and P<0.001, respectively). The zymogram of cytosolic fractions identified a protein of 170kDa containing the carboxyleste...

The cattle tick Rhipicephalus microplus, is one of the most damaging livestock ectoparasites. Tropical tick infestation limits the introduction of high-yield bovine varieties because they do not have immunity to the diseases transmitted by these ectoparasites. This tick is usually controlled with chemical acaricides but their indiscriminate use has...

The purpose of this study was to describe an unreported entomopathogenic fungus that naturally infects the cattle tick Rhipicephalus (Boophilus) microplus (Acari: Ixodidae). Engorged female ticks, showed symptoms of fungal infection after controlled tick infestation of cattle. Infected ticks developed a distinctive dark colour, a pale mould grew ov...

The purpose of this book is to convey the available knowledge on cell cultures based on the expertise of the authors themselves, to those Spanish speaking persons that are interested in this subject. This book contains information on basic data, metrical units, proper nomenclature and appropriate definitions to simplify its understanding. Similarly...

Different laboratory cultures of the acarine tick Rhipicephalus (Boophilus) microplus (Canestrini, 1888) (Ixodida: Ixodidae) were infested by small Megaselia scalaris (Loew, 1866) (Diptera: Phoridae) flies. Larvae of this species exhibited opportunistic parasitism predominantly on engorged female ticks, causing severe damage to their cuticle throug...

During experimental Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) infestation on cattle, approximately 5% of engorged female ticks showed symptoms of bacterial infection. The affected ticks were unable to oviposit, secreted a distinctive yellow exudate through the genital orifice and eventually died. Microscopic analysis of tick exudate sho...

Abstract: An acaricide-resistant and an acaricide-susceptible strain of Rhipicephalus microplus ticks, were evaluated during a 40 day larval development period for carboxylesterase expression on SDS-PAGE, with the objective of establishing the optimal larval age for carboxylesterase specific activity measurement. Experimental results demonstrated t...

The present study reports the cloning and expression of a Caenorhabditis elegans Cathepsin B-like Protease (CBP), with the objective of obtaining a recombinant enzyme bearing biochemical properties similar to natural CBP reported in the literature for C. elegans and parasitic nematodes. The gene was isolated by PCR from C. elegans cDNA, resulting a...

A Haemonchus contortus recombinant Cysteine Protease (CP) was expressed in the baculovirus system. The CP gene was isolated by PCR from H. contortus cDNA, the PCR amplicon was cloned downstream to the polihedrin promoter within a bacterial expression vector, Sf9 insect cells were used for simultaneous co-transfection with the CP-vector and baculovi...

The aim of this research was to evaluate the efficacy of a combined method of parasitic control, with albendazol and Duddingtonia flagrans chlamydospores in Haemonchus contortus artificially infected sheep. Fourty H. contortus infected sheep, were used. This experiment was carried out in a 90 d period. Sheep were ramdomly divided into four groups o...

Abstract: This study was done with the objective of establishing a relationship between cytochrome P-450 gene expression and acaricide resistance levels in Mexican isolates of R. microplus, for that purpose the cytochrome P-450 expression levels were measured by real time PCR quantification on acaricide sensitive and resistant strains of ticks, as...

This study was made to assess SDS-PAGE monooxygenases zymograms in the presence of synthetic monooxygenase enzyme substrates, in order to establish a reliable methodology to measure monooxygenase activity levels on acaricide resistant ticks and finding a probable association between monooxygenase enzymatic levels and B. microplus acaricide resistan...

Anthelmintic resistance detection of ruminant gastroenteric parasitic nematode Heamonchus contortus, can be achieved by molecular probes and DNA amplification assays, this advanced methodologies are faster and cheaper to apply than conventional bioassays, however for better accuracy of a DNA-based test, it is necessary first to clarify the details...

Se evaluó un método antiparasitario combinado, mediante el uso de albendazol y del hongo Duddingtonia flagrans, en ovinos infectados con Haemonchus contortus. El experimento duró 90 días. Se formaron cuatro grupos de 10 corderos cada uno, infectados con H. contortus y recibieron los siguientes tratamientos: Grupo 1, albendazol (7.5 mg/kg), a los dí...

Rhipicephalus microplus in vitro cell culture have been useful in the study of acaricide resistance in ticks, a methodology for heterologous gene transference into these cultures may help to study several aspect of the cell biology and gene expression associated with acaricide resistance. In this study we assessed a pantropic retroviral gene transf...

We report a molecular cloning and expression in the bacu- lovirus system of an Haemonchus contortus recombinant cystein protease (CP) gene, with the objective of obtaining a recombinant CP with biochemical and immunochemical properties similar to the natural one obtained from the parasite, with applications to the diagnosis prevention and control o...

La identificación de resistencia a los antihelmínticos del nematodo parásito Haemonchus contortus, se hace mediante un complicado bioensayo que requiere larvas de nematodos de referencia, así como de campo, cultivadas en laboratorio y expuestas a diferentes productos. Con el fin de evaluar una posible alternativa diagnóstica, se hicieron ensayos en...

Phospholipid-hydroperoxide glutathione peroxidase (PHGPx) enzymes are associated with cellular protection by the role they play in reducing hydroperoxides of phospholipids, thereby preventing membrane lipoperoxidation. As part of their toxic effect, some pesticides stimulate peroxidation of cellular membranes. We isolated and sequenced a PHGPx gene...

La identificación de resistencia a los productos acaricidas en la garrapata B. microplus, se hace mediante un complicado bioensayo, que requiere garrapatas de referencia y de campo cultivadas sobre bovinos vivos y restringidos durante semanas. Con el fin de evaluar una posible alternativa diagnóstica, se hicieron ensayos enzimáticos en geles de pol...