Elvira Gonzalez de Mejia

University of Illinois, Urbana-Champaign | UIUC

Jatropha curcas has been used mainly to obtain biofuels from the oil of the seeds. During the oil extraction, a residual paste is generated with a high percentage of proteins, including curcin, related to its antimicrobial effect due to its rRNA N-glycosidase activity. The present investigation focuses on characterizing gold nanoparticles with curc...

The objective of this study was to evaluate germinated chickpea protein hydrolysate (GCPH) in vitro for its effect on markers of type 2 diabetes (T2D) and bitter taste receptor expression in intestinal epithelial cells. Protein hydrolysate was obtained using ficin, and the resulting peptides were sequenced using LC-ESI-MS/MS. Caco-2 cells were used...

Soybean compounds have been established to modulate inflammation, but less is known about how whole soybean compositions work together after digestion. The objective was to evaluate and compare the anti-inflammatory responses of different soybean varieties under simulated gastrointestinal digestion, with additional consideration of the glycinin:β-c...

As a protein source, chia contains high concentrations of bioactive peptides. Probiotics support a healthy digestive tract and immune system. Our study evaluated the effects of the intra-amniotic administration of the hydrolyzed chia protein and the probiotic Lacticaseibacillus paracasei on intestinal bacterial populations, the intestinal barrier,...

Oxidative stress and inflammation play a key role in diverse pathological conditions such as cancer and metabolic disorders. The objective of this study was to determine the antioxidant and anti-inflammatory potentials of crude extract (CE) and phenolic-enriched extract (PHE) obtained from the seed coats (SCs) of black bean (BB) and pinto bean (PB)...

This research evaluated the association between the different protein and peptide composition of seven soybean varieties and their radical scavenging and antioxidant properties throughout simulated gastrointestinal digestion. Glycinin and β-conglycinin were the primary and more varying proteins among soybean varieties. Protein composition determine...

The objective of this study was to assess the effectiveness of liposomes loaded with soybean lunasin and amaranth unsaponifiable matter (UM + LunLip) as a source of squalene in the prevention of melanoma skin cancer in an allograft mice model. Tumors were induced by transplanting melanoma B16-F10 cells into the mice. The most effective treatments w...

Inflammation is a normal response in defense to agents that may cause damage to the human body. When inflammation becomes chronic, reactive oxygen species (ROS) are produced; which could lead to diseases such as cancer. The aim was to assess liposomes’ antioxidant and anti-inflammatory capacity loaded with amaranth unsaponifiable matter and soybean...

Chronic inflammation refers to long-lasting inflammation that occurs over a period of several months to years, and it is associated with the progression of other chronic diseases. It may be induced by alcohol consumption and a high-fat diet. Soybean bioactive compounds prevent chronic inflammation by primarily targeting the nuclear factor kappa B (...

Objectives The objective was to assess the mechanism of action of liposomes loaded with amaranth unsaponifiable matter and lunasin (LpLnUM) in melanoma tumors developed in C57BL/6 mice. Methods Tumors were developed in C57BL/6 using the melanoma cell line B16-F10 and treated every other day for 22 days using LpLnUM with 15 (LpLnUM15) or 30 (LpLnUM...

Objectives To evaluate the effect of an optimized germinated chickpea protein hydrolysate (GCPH) on markers of type-2 diabetes such as dipeptidyl peptidase-IV (DPP-IV) inhibition, glucose uptake and expression of glucose transporters in enterocytes and their relationship to bitter taste receptor expression. Methods GCPH was characterized using LC-...

Objectives To evaluate the efficacy of a chickpea (Cicer arietinum) protein hydrolysate (CPH) on ameliorating markers of obesity in a mouse model of metabolic dysfunction when fed a high fat diet. Methods CPH was produced using an Alcalase® and Flavourzyme® dual-system. Fifty-two male C57BL/6J mice (6 weeks of age) were randomized into four groups...

Objectives This study aimed to investigate the effect of the main bioactive compounds from the coffee silverskin and coffee husk on hepatic lipid and glucose metabolism and mitochondrial bioenergetics using an in vitro model mimicking non-alcoholic fatty liver disease. Methods HepG2 cells were treated with 50 μmol L−1 caffeine, chlorogenic, caffei...

Background and Aim Overweight and obesity are associated with the development of several health complications, such as diabetes, cancer, and cardiovascular disease. Chia seed is a rich source of proteins and peptides with potential anti-inflammatory and antioxidant effects. This study aimed to evaluate the effects of digested proteins (DP) from chi...

The potential of pentapeptide IPQVS (RAP1) and octapeptide ELHQEEPL (RAP2) derived from rapeseed napin as natural dipeptidyl-peptidase IV (DPP-IV) inhibitors is promising. The objective was to develop a nanogel strategy to resist the hydrolysis of digestive and intestinal enzymes to enhance the DPP-IV inhibitory activity of RAP1 and RAP2, and stimu...

Common bean has the potential to improve gut microbiota function due to its chemical composition and content of dietary fiber. This study evaluated the effect of Cooked Common Bean (CCB) flour and its protein hydrolysate as part of a high-fat diet (HFD) added with 6-propyl-2-thiouracil (PTU) (10 mg/kg/day), an inhibitor of thyroid hormone synthesis...

Coffee by-products contain bioactive compounds that have been shown to have the capacity to modulate human metabolism. The goal of this study was to investigate the effects of the main bioactive compounds in coffee by-products and two aqueous extracts from the coffee husk and silverskin on the activation of fibroblast growth factor 21 (FGF21) signa...

Coffee consumption has been associated with the reduction of several chronic diseases, including type 2 diabetes mellitus (T2DM) and obesity. The aim of this review was to summarize the research conducted in the last five years (or older, when appropriate) on the relationship between the consumption of coffee bioactive compounds, obesity, and T2DM....

The cocoa shell is a by-product that may be revalorized as a source of bioactive compounds to prevent chronic cardiometabolic diseases. This study aimed to investigate the phytochemicals from the cocoa shell as targeted compounds for activating fibroblast growth factor 21 (FGF21) signaling and regulating non-alcoholic fatty liver disease (NAFLD)-re...

The objective was to optimize and compare the production of antidiabetic peptides from germinated chickpea isolated protein using either papain or ficin. Kabuli chickpeas were germinated for 2, 4 and 6 days. Proteins were isolated, and peptides were produced based on a central composite design selecting human dipeptidyl peptidase (DPP-IV) inhibitio...

Chickpea hydrolysates have shown bioactivity towards type 2 diabetes by inhibiting dipeptidyl peptidase (DPPIV) activity. The objective was to compare the effect of adding different levels of an optimized bromelain hydrolysate from chickpea isolated protein on DPPIV inhibition capacity and physicochemical properties of maize tortilla. White and blu...

Amaranthus hypochondriacus is a source of molecules with reported health benefits such as antioxidant activity and cancer prevention. The objective of this research was to optimize the conditions for preparing a liposome formulation using amaranth unsaponifiable matter as a source of squalene in order to minimize the particle size and to maximize t...

This research aimed to assess the impact of an aqueous extract from the cocoa shell and its major phytochemicals on preventing oxidative stress and mitochondrial dysfunction in hepatocytes using an in vitro model of nonalcoholic fatty liver disease (NAFLD). The phytochemicals from cocoa shell were extracted using water and characterized by UPLC-MS/...

The objective was to compare five varieties of chickpea (Cicer arietinum), sequence the peptides obtained with pepsin-pancreatin digestion, and evaluate their potential as modulators of biochemical markers for type-2 diabetes. In addition, to produce a functional ingredient, by the optimization in the production of hydrolysates using bromelain. Pro...

Objectives This research aimed to evaluate the effect of the consumption of a baked corn and bean snack in the intestinal oxidative stress and energetic metabolism in chronic colitis in vivo. Methods The polyphenolic composition of baked 70% nixtamalized corn (Zea mays L.) and 30% cooked common bean (Phaseolus vulgaris L.) snack (70/30) was charac...

Objectives To produce and evaluate the microstructure and rheological behavior of a nanogel of pentapeptide IPQVS (RAP1) and octopeptide ELHQEEPL (RAP2), derived from rapeseed napin, through RADA16-assisted molecular design. In addition, to determine in vitro the dipeptidyl-peptidase IV (DPP-IV) inhibitory properties, glucagon-like peptide-1 (GLP-1...

Objectives To evaluate the effects of whole flour and protein hydrolysate from common bean on gut health in mice fed a High-Fat Diet. Methods BALB/c adults mice (n = 48) where divided in 4 groups (n = 12 each): normal control (NC standard diet AIN-93M); High-Fat Diet (HFD) only; HFD plus bean flour (HFBF) and HFD plus bean protein hydrolysate (HFP...

Chickpea (Cicer arietinum) is one of the most consumed pulses worldwide (over 2.3 million tons enter the world market annually). Some chickpea components have shown, in preclinical and clinical studies, several health benefits, including antioxidant capacity, and antifungal, antibacterial, analgesic, anticancer, antiinflammatory, and hypocholestero...

Lunasin has demonstrated antioxidative, anti-inflammatory, and chemopreventive properties. The objectives were to evaluate the concentration of lunasin in different lunasin-based commercial dietary supplements, to produce a lunasin-enriched soy extract (LESE) using a two-step pilot-plant-based ultrafiltration process, and to evaluate their biologic...

The use of pulses, such as chickpea, has become more relevant in baking as they exhibit potential health benefits such as reduction of obesity, type 2 diabetes, and prevention of colon cancer. It is also a good source of highly bioavailable protein at a low cost. This allows companies to develop new innovative products that meet the demand for nutr...

The objective was to evaluate the mechanisms of digested total proteins (DTP), albumin, glutelin, and pure peptides from chia seed (Salvia hispanica L.) to prevent adipogenesis and its associated inflammation in 3T3-L1 adipocytes. Preadipocytes (3T3-L1) were treated during differentiation with either DTP or digested albumin or glutelin (1 mg/mL) or...

This chapter compiled references from the scientific literature to understand the role of anthocyanins in oxidative stress and the potential prevention of gastrointestinal cancer. The types of cancer searched were present in the esophagus, stomach, small intestine, colon, rectum, anus, liver, gallbladder, and pancreas. It is well known that chronic...

Animal studies can increase the accuracy in revealing the molecular mechanisms of bioactive peptides from food sources regarding specific disease prevention. However, so far, methods of animal models for assessing active food peptides are not well classified. For instance, the most effective route of administration, the dosage selection of active p...

The chickpea (Cicer arietinum L.) is one of the most important pulses worldwide. The objective was to identify, compare and evaluate peptides from chickpea hydrolysates produced by two enzymatic treatments. The antidiabetic potential and bitterness of the peptides and induction of bitter receptors were identified in silico. Proteins were isolated f...

Bioactive compounds from food products made from natural ingredients such as corn and common bean could target the NLRP3 inflammasome, protein scaffolds with a key role in the moderation of intestinal inflammation. This research aimed to evaluate the anti-inflammatory effect from the fermented non-digestible fraction of baked corn and common bean s...

This study aimed to assess the effect of the primary phytochemicals from coffee by-products and two aqueous extracts from the coffee husk and silverskin on lipid and glucose metabolism regulation in hepatocytes using an in vitro model of non-alcoholic fatty liver disease. Coffee husk and silverskin were used to prepare two aqueous extracts (CHE and...

The objective was to investigate the anti-adipogenesis potential of selected legume protein hydrolysates (LPH) and combinations using biochemical assays and in silico predictions. Black bean, green pea, chickpea, lentil and fava bean protein isolates were hydrolyzed using alcalase (A) or pepsin/pancreatin (PP). The degree of hydrolysis ranged from...

The objective was to characterize the phytochemical profile of Moringa oleifera leaves extracts (aqueous, AE; methanolic, ME; glucosinolates, GE; and glucosinolate-rich hydrolyzed, GEH) and comparatively evaluate their antiproliferative effect on HCT116 and HT-29 human colorectal cancer cells. Moringa extracts were composed of chlorogenic acid, que...

The skin is the largest organ in the human body, providing a barrier to the external environment. It is composed of three layers: epidermis, dermis and hypodermis. The most external epidermis is exposed to stress factors that may lead to skin conditions such as photo-aging and skin cancer. Some treatments for skin disease utilize the incorporation...

The objective of this study was to investigate the biochemical antioxidant potential of peptides derived from enzymatically hydrolyzed mung bean (Vigna radiata) albumins using an 2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging assay, a ferrous ion chelating assay and an oxygen radical absorbance capacity (ORAC) assa...

Ulcerative colitis (UC) is a condition that has been rising in the number of cases around the world. Food products made from natural ingredients such as corn and common bean might serve as alternatives for the treatment of UC. This study aimed to assess the anti-inflammatory effect of the consumption of a baked corn and bean snack (CBS) in an in vi...

Objectives To evaluate the mechanism of action of the effect of digested total proteins (DTP), albumin, and glutelin from chia seed to prevent and inhibit inflammation in 3T3-L1 adipocytes. Methods Preadipocytes (3T3-L1) were differentiated into mature adipocytes and received DTP, digested albumin, or glutelin (1 mg/ml) from chia seed together wit...

Objectives To evaluate the effect of selected bioactive compounds from cocoa shell and its aqueous extract on the activation of fibroblast growth factor 21 (FGF21) signaling in hepatocytes and the resulting modulation of lipid and glucose metabolism, oxidative stress, and mitochondrial function. Methods The bioactive compounds from cocoa shells we...

Objectives The objective was to compare the impact of black lentil water extract (BL) and delphinidin 3-O-(2-O-β-d-glucopyranosyl-α-l-arabinopyranoside) (D3G)-rich lentil extract on tumor development and inflammation in an azoxymethane (AOM)/dextran sodium sulfate (DSS) model. Methods C57BL/6 mice were randomly separated into four groups: healthy...

Objectives To compare the anti-obesity efficacy of different concentrations of phenolic-rich water extract from purple maize pericarp (PPE) in a murine model of obesity for 12 weeks. Methods Forty C57BL/6 mice (n = 10/group) were randomized: standard diet (SD), high-fat diet (HFD), HFD + 200 mg PPE/kg BW (200 PPE), and HFD + 500 mg PPE/kg BW (500...

Objectives To evaluate the effects of digested total proteins (DTP) from chia seed to prevent adipogenesis and adipose tissue inflammation in mice fed a high-fat diet. Methods C57Bl/6 black mice (n = 44; 8 weeks old) where divided in 4 groups (n = 12 each): negative control (NC; standard diet AIN-93M); positive control (PC; high fat diet- HFD- 60%...

Objectives To investigate the impact of cyanidin-3-O-glycoside (C3G) and its two major phenolic acid metabolites, protocatechuic acid (PCA) and ferulic acid (FA), on macrophage (MФ) factor-induced inflammation and mitochondrial dysfunction in 3T3-L1 adipocytes. Methods Secretory factors from lipopolysaccharide (LPS)-stimulated RAW 274.7 MФ were us...

Soy protein and corn meal are widely used as main ingredients in the cereal and snack industries. We studied the effect of extrusion processing on the immunoreactivity of proteins in soy protein isolate-corn meal (SPI-corn) blends and soy flour-corn meal (SF-corn) blends using a full-factorial design with two levels of temperature, moisture, and sc...

The aim was to compare the anti-obesity efficacy of different concentrations of phenolic-rich water extract from purple maize pericarp (PPE) in a murine model of obesity for 12 weeks. Forty C57BL/6 mice (n=10/group) were randomized: standard diet (SD), high-fat diet (HFD), HFD+200 mg PPE/kg (200 PPE), and HFD+500 mg PPE/kg (500 PPE). PPE contained...

The effects of anthocyanins and concomitant compounds on adipogenesis-related disorders in adipose tissue remain unclear. This study aimed to investigate the impacts of cyanidin-3-O-glycoside (C3G), protocatechuic acid (PCA) and ferulic acid (FA), on macrophage (MФ) factor-induced inflammation and mitochondrial dysfunction in 3T3-L1 adipocytes. In...

The objective was to compare the impact of black lentil water (BL) and delphinidin 3-O-(2-O-β-d-glucopyranosyl-α-l-arabinopyranoside) (D3G)-rich lentil extracts on tumor development, inflammation, and immune response in an azoxymethane (AOM)/ dextran sodium sulfate (DSS) model. C57BL/6 mice were randomly separated into four groups: healthy control...

The objective was to examine the effectiveness of mate tea (MT, Ilex paraguariensis St. Hilaire) and caffeine from mate tea (MC) on in vitro lipid accumulation and in vivo diet-driven-obesity. MC and decaffeinated mate (DM) were obtained using supercritical CO2 extraction and mainly composed of caffeine and caffeoylquinic acids, respectively. MC re...

There is an increasing consumer demand for natural colors in foods. However, there is a limited number of available natural food sources for use by the food industry because of technical and regulatory limitations. Natural colors are less stable and have less vibrant hues compared to their synthetic color counterparts. Natural pigments also have kn...

Chickpeas are inexpensive, protein rich (approximately 20% dry mass) pulses available worldwide whose consumption has been correlated with positive health outcomes. Dietary peptides are important molecules derived from dietary proteins, but a comprehensive analysis of the peptides that can be produced from chickpea proteins is missing in the litera...

The aim of this study was to evaluate the inhibitory potential of aqueous extracts from coffee silverskin (CSE) and husk (CHE) and their main phenolics on adipogenesis, obesity-related inflammation, mitochondrial dysfunction, and insulin resistance, in vitro. Coffee by-products extracts (31-500 μg mL-1) and pure phenolics (100 μmol L-1) reduced lip...

Blackcurrants (BC; Ribes nigrum) are relatively new to the U.S. market; however, they are well known and popular in Europe and Asia. The use of BC has been trending worldwide, particularly in the United States. We believe that demand for BC will grow as consumers become aware of the several potential health benefits these berries offer. The objecti...

The objective was to assess anti-progression and stimulatory immune response effects among anthocyanins (ANC) and their metabolites on human colorectal cancer cells in vitro and in silico. Pure phenolics including delphinidin-3-O-glucoside (D3G) and its metabolites, delphinidin (DC) and gallic acid (GA), were tested alone or in combination, on HCT-...

This study aimed to compare the phytochemicals from coffee and cocoa by-products and their relationship with the potential for reducing markers of inflammation, oxidative stress, adipogenesis, and insulin resistance in vitro. We characterized the phytochemical profile of extracts from coffee husk, coffee silverskin, and cocoa shell and evaluated th...

We aimed to compare the composition-bioactivity profile of coffee and cocoa by-products by comprehensively characterizing the composition of extracts from coffee husk, coffee silverskin, and cocoa shell and evaluating their in vitro biological activity in RAW264.7 macrophages and 3T3-L1 adipocytes. Coffee husk and coffee silverskin extracts were ma...

Scope: The objectives were to evaluate the anti-inflammatory and anti-atherosclerotic effects of digested total protein and digested protein fractions from chia seed in macrophages in vitro. Methods and results: Total protein and protein fractions (albumin, globulin, glutelin and prolamin) were isolated from chia seed and digested using simulate...

Objectives: Food-based products such as common bean peptides may contribute to reduce the risk of complications associated with obesity. The objective of this research was to produce and characterize peptides from common bean protein isolates and evaluate their potential to prevent adipogenesis. Methods: Protein hydrolysates of raw common black...

Objectives: The objective was to evaluate the effect of an aqueous phenolic extract from cocoa shell (CAE) and its main phenolic compounds to prevent the adipogenesis-induced loss of mitochondrial function and insulin sensitivity, targeting the inflammatory interplay macrophages-adipocytes in vitro. Methods: CAE and its main phenolic compounds (...

Objectives: The aim of this research was to evaluate the chemopreventive activity of Moringa oleifera(MO) leaves in a colitis-associated colon carcinogenesis model. Our hypothesis was that moringa leaves contain bioactive compounds capable of inducing antioxidant systems, reducing the incidence of colonic lesions, as well as modulating the express...

Objectives: The aim of this research was to evaluate the anti-inflammatory properties of an oven-baked nixtamalized corn (Zea mays L.)/cooked common bean (Phaseolus vulgaris L.) chip in a model of chronic colitis in vivo. The hypothesis was that the chip consumption would prevent colon barrier disruption and improve enzymatic biomarkers by the mod...

Objectives: The objective was to compare the efficacy of a black lentil aqueous extract (BL) and a semi-purified anthocyanin (ANC) extract to prevent tumor development and inflammatory processes and impact on immune response in an azoxymethane (AOM)/dextran sodium sulfate (DSS) mouse model. Methods: BL was obtained from an overnight soak of orga...

The incidence of these metabolic disorders is increasing worldwide primarily due to poor lifestyle habits. Obesity is caused by the imbalance between energy intake and expenditure and entails the storage of excess amounts of triglycerides, associated with the production of adipokines involved in inflammation. Likewise, the accumulation of macrophag...

Mol. Nutr. Food Res. 2019, 63, 1801413 DOI: 10.1002/mnfr.201801413 In article number 1801413 by Elvira Gonzalez de Mejia and co‐workers, an aqueous extract from cocoa shell and its main phenolics reduces inflammation derived from the interaction between macrophages and adipocytes. Adipocytes show a reduction in the accumulation of fat. Similarly, p...

Scope: The aim was to assess the action of an aqueous extract from cocoa shell (CAE) and its main phenolic compounds to prevent the loss of obesity‐induced mitochondrial function and insulin sensitivity, targeting inflammation between macrophages‐adipocytes in vitro. Methods and results: CAE (31‐500 μg mL−1) inhibited 3T3‐L1 adipocytes lipid accum...

Relationship of phenolic composition of selected purple maize (Zea mays L.) genotypes with their anti-inflammatory, anti-adipogenic and anti-diabetic potential, Food Chemistry (2019), doi: https://doi. Abstract This study aimed to investigate the associations between phenolic composition of selected purple maize genotypes and their anti-inflammator...

This study aimed to investigate the associations between phenolic composition of selected purple maize genotypes and their anti-inflammatory, anti-adipogenic and anti-diabetic properties in vitro. Anthocyanin-rich water extracts (PMWs) from 20 purple maize genotypes were evaluated in RAW 264.7 macrophages and 3T3-L1 adipocytes under different condi...

Background and objectives Purple corn can be an economical source of anthocyanins, a natural alternate to synthetic FD&C Red 40 dye in foods. The effects of steeping purple corn pericarp in wet milling chemicals, namely sodium metabisulfite and lactic acid, and milling on the extraction of anthocyanins, polyphenols, flavonoids and tannins were stud...

The aim was to characterize a phenolic-rich water extract from the pericarp of an improved genotype of Apache red maize (RPE), and evaluate its ability to activate the type-2 diabetes markers free fatty acid receptor 1 (GPR40) and glucokinase (GK) in vitro. The extract contained mainly phenolic acids, anthocyanins, and other flavonoids. RPE inhibit...

The prevention of obesity and its related inflammatory effects using natural compounds has gained attention. The aim was to compare the effect of an anthocyanin‐rich extract from purple corn pericarp (PCW) and pure anthocyanins on 3T3‐L1 adipocytes on basal and inflammatory conditions. PCW had mainly cyanidin‐3‐ O‐ glucoside (C3G) and in a lesser p...

Foods associated with lowering risk of colorectal cancer include fruits, vegetables, and whole cereals and legumes. Anthocyanins (ANC), pigments in the flavonoid class, have shown potential to provide various health benefits, including prevention and suppression of colon cancer. The objective of this study was to evaluate the anti‐proliferative eff...

Elevated circulating lipid levels are known risk factors to develop cardiovascular diseases (CVD). Diets rich in whole grains, fruits, vegetables and legumes have been linked to a reduced risk of CVD. The objective was to evaluate the effects of corn and bean snack consumption on lipid metabolism. C57BL/6J mice were randomized into 3 groups: 1) Two...

Celiac disease is an autoimmune disorder that affects the small intestine. When gluten, a protein found in wheat, rye and barley is consumed, the immune system responds by targeting the small intestine and inhibiting the absorption of nutrients into the body. The objective was to evaluate the potential of anthocyanin‐rich plant extracts to protect...

Black bean coats are a rich source of anthocyanins, natural pigments that could be used as food colorants with outstanding biological potential. The objective was to extract and characterize the black bean coat anthocyanins and evaluate their antidiabetes potential in an in vitro model. The anthocyanin‐rich extracts (ARE) were characterized using L...

The objective of this study was to evaluate the ability of anthocyanins (ANC) present in purple corn to enhance insulin secretion and hepatic glucose uptake in pancreatic cells and hepatocytes, through activation of the free fatty acid receptor-1 (FFAR1) and glucokinase (GK), respectively. Using a dual-layer cell culture with Caco-2 cells, INS-1E o...

This article reviews recent developments in methods of sample preparation and analytical methodologies for the quantification of anthocyanins and their extraction from food sources. Various methods for sample extraction and purification are highlighted and evaluated. The use of UV-diode array, along with improved liquid chromatography (LC) and mass...

The effect of amaranth peptides on the activity and expression of tissue factor (TF) on THP-1 activated cells was evaluated in vitro. An active anticoagulant peptide fraction (AF) was found to inhibit TF expression (IC50 = 0.39 mg/mL) and activity. Immunocytochemical fluorescence confocal microscopy analysis showed that treated monocytes decreased...

The objective was to evaluate the effect of black bean protein fraction (PFRA), and its derived peptides on glucose uptake, SGLT1 and GLUT2 expression and translocation on Caco-2 cells. The effect of treatments was evaluated on glucose uptake, protein expression and localization and gene expression on Caco-2 cells. PFRA (10 mg/mL) lowered glucose u...

Legume seeds of the Fabaceae family are known to contain bioactive compounds with anticancer potential. Gastrointestinal (GI) cancer is one of the most common types of cancer closely related to dietary habits. Molecular mechanisms by which peptides from legumes exert their anticancer effect have been only partially elucidated such as the interactio...

The objective was to compare the chemical stability and color of nine unique anthocyanin-rich colored corn varieties named/coded as V1, V2, V3… V9. Extracts were added to a beverage model and stored at 4 °C, 22 °C, or 32 °C for 12 weeks. After 12 weeks of storage at 32 °C, variety V6 [high condensed form (CF), high cyanidin-3-O-glucoside (C3G)] had...

The aim was to compare the distribution of ANCs in purple and blue corn coproducts from three conventional corn fractionation processes and linking ANC partitioning in different coproducts to corn kernel phenotype. Total monomeric anthocyanin (TA) from purple corn extract was 4933.1 ± 43.4 mg cyanidin-3-glucoside equivalent per kg dry corn, 10 time...

The objective was to compare the anti-proliferative effect of anthocyanin-rich plant extracts on human colon cancer cells and determine their mechanism of action. Eleven extracts were tested: red (RG) and purple grape, purple sweet potato, purple carrot, black and purple bean, black lentil (BL), black peanut, sorghum (SH), black rice, and blue whea...

The aim was to evaluate the antineoplastic potential of a previously characterized peptide extract from the non-digestible fraction of common bean cv. Azufrado Higuera (AH) and its most abundant pure peptide GLTSK, in an azoxymethane/dextran sodium sulfate (AOM/DSS)-induced colitis-associated colon carcinogenesis Balb/c mice model. The healthy cont...

The objective was to develop a 100 g lab scale dry milling protocol to determine effects of corn cultivars on coproduct fraction yields and composition. Six yellow dent, three colored and one white cultivar of corn were processed using 100 g samples to generate six coproducts, namely large grits, medium grits, small grits, fines, germ and pericarp....