Elhadi Ahmed

University of Gezira · Department of Medical Microbiology, Faculty of Medical Laboratory Sciences

Introduction: The level of immune response to hepatitis B virus vaccine is an indicator of whether the expected protection has been achieved in the target population. Objective: the study aimed to determine the immune response to recombinant hepatitis B virus rDNA among students in two medical laboratory colleges in Wad Madani city in Sudan. Method...

Background: Implementing safety and personnel management procedures in medical laboratories reduces the risks expected during work Objectives: The current study aimed to evaluate safety and personnel practices in medical laboratories in Wad Medani City, Sudan Methods: A cross-sectional analytical design based on the participation of medical laborat...

Background: Implementing safety and personnel management procedures in medical laboratories reduces the risks expected during work Objectives: The current study aimed to evaluate safety and personnel practices in medical laboratories in Wad Medani City, Sudan Methods: A cross-sectional analytical design based on the participation of medical laborat...

Background: Hepatitis E virus (HEV) infection is an increasing global problem and high endemicity is observed in many developing countries. Pregnancy predisposes women to chronic infection and various complications. Objective: This study aimed to investigate sero-positivity of HEV and existent risk factors among pregnant women in Gezira State, Suda...

Background: The quinolone group, a synthetic antimicrobial, is widely used worldwide to treat many diseases, including those caused by Gram-negative bacteria. Escherichia coli and others are among the bacteria that produce quinolone resistance genes (qnr) such as qnrA and aac(6′)-Ib-cr. Objective: The present study aimed to the isolate Escherichia...

Introduction: The variation in symptoms and severity of COVID-19 among patients could be linked to genetic issues, including defects in the renin-angiotensin system (RAS) pathway. The ACE1 Insertion/Deletion (I/D) polymorphism gene, which regulates the RAS pathway, may also explain the genetic susceptibility to COVID-19 and its association with oth...

Introduction: The diversity of symptoms, signs, and severity in COVID-19 patients has been attributed to various factors, including hereditary issues such as the renin-angiotensin system (RAS). The ACE1 Insertion/Deletion (I/D) polymorphism has been considered a genetic risk factor for COVID-19 and its associated disorders. This case-control hospit...

Background: Internal quality control (IQC) it is all the steps taken by the staff inside the laboratory included improve performance, equipment's, reagents, and operational processes to insure the credibility of results. Objective: To analyze the internal quality control practice, knowledge and altitudes of medical laboratory personal among partici...

Background: Early prostate cancer antigen (EPCA), a nuclear matrix protein, has recently been recommended as a hopeful biomarker for early prostate carcinogenesis. Objectives: To evaluate the validity of serum early prostate cancer antigen (EPCA) in the early detection of prostate cancer (PCa) and the characteristics of Sudanese patients diagnosed...

Background: Chlamydia trachomatis (CT) is a sexually transmitted pathogen that threatens reproductive health worldwide. This study aims to screen CT urogenital infection using cytology and molecular methods in women suffering infertility. Methods: In total, 415 women suffering infertility, attending Wad Madani Maternity Hospital were included in th...

Background: Leukemia frequently causes anemia; thus patients need products containing normal blood or RBCs for treatment. Anti-red blood cell alloantibody formation is still a critical challenge in transfusion. Objectives: The current study aimed to investigate RBC alloantibodies in Sudanese leukemia patients who receive multiple blood transfusi...

Background: Oral candidiasis is the most common fungal infection in immune compromised individuals such as AIDS patients. Candida albicans alone is responsible of more than 95% of candidiasis. The increase prevalence of oral candidiasis among African HIV infected people has resulted in increased in usage of antifungal agents for both prophylactic a...

Introduction: Transmission of hepatitis B virus (HBV) through the blood is a significant risk, especially in poor countries with high endemicity. Occult transmission of HBV (OBI) is an important acquisition scenario. Objective: A cross-sectional laboratory-based study followed to detect OBI in blood of accepted donors admitted to Mad Medani Blood B...

Background: Hepatitis E virus (HEV) is a hepatotropic pathogen that causes significant morbidity and mortality in humans. It is an important causative agent of viral hepatitis outbreaks. This study investigates the serological and molecular prevalence of HEV in blood donors attending the Central Blood Bank in Wad Medani City in Gezira State, Sudan....

Investment in SARS-CoV-2 sequencing in Africa over the past year has led to a major increase in the number of sequences generated, now exceeding 100,000 genomes, used to track the pandemic on the continent. Our results show an increase in the number of African countries able to sequence domestically, and highlight that local sequencing enables fast...

The human epidermal growth factor receptor-2 (HER2), which is expressed in large amount in 20%-30% of breast cancer patients, is associated with disease recurrences, shortened disease survival, and bad prognosis. The aim of this study is to evaluate the expression of (Her2/neu) receptor as a diagnostic and prognostic marker in Invasive Ductal Breas...

Background: Despite the great effort of the malaria control program in Sudan, Plasmodium vivax malaria has remained a major challenge recently, causing significant morbidity with a variety of haematological changes. Objective: This study aims to investigate the effect of Plasmodium vivax malaria and their density on some haematological parameters i...

Background: The characteristics of Staphylococcus aureus that made it the most important cause of wound infections are environmental spread antimicrobials resistance and virulence. Absence of molecular detection of drug resistance and virulence factors in many developing countries limits the epidemiological information. This study conducted to iden...

Investment in Africa over the past year with regards to SARS-CoV-2 genotyping has led to a massive increase in the number of sequences, exceeding 100,000 genomes generated to track the pandemic on the continent. Our results show an increase in the number of African countries able to sequence within their own borders, coupled with a decrease in sequ...

Background: Direct isolation of genomic DNA of mycetoma fungi from black-grains achieve rapid diagnosis and may overcome culture disadvantages. Objectives: This study aimed to isolate and assess the DNA of mycetoma fungi using black-grains and to apply amplification of ITS region and nucleotide sequences. Methods: CTAB method was followed by manual...

Background: Epstein-Barr virus (EBV) infects around 95% of the world's population. This infection has been linked with lymphoid and epithelial cancers; persistence of infection in lymphoid or epithelial cells may result in virus-associated B-cell tumors or nasopharyngeal carcinomas (NPC). Objective: The study aimed to detect Epstein-Barr virus anti...

Mycetoma is one of neglected tropical diseases that threat every individual live or work in mycetoma endemic areas. Black-grain mycetoma is the commonest type of mycetoma in Africa. In Sudan Madurella mycetomatis alone is responsible of more than 70% of all mycetoma cases. The cross-sectional hospital based study was carried out from 2015 to 2017 i...

Background: Most community and hospital acquired infections are due to Staphylococcus aureus, these infections involve both local suppurative and systemic conditions. There is an increase in the incidence rates of acquiring resistant strains of Staphylococcus aureus in hospitals and community in Sudan. This study aims to detect frequency of Staphyl...

In our previous study, it was shown that endogenous vitamin D3 and its metabolites are associated with diabetic microvascular complications and cardiovascular risk factors. The aim of the present study was to determine if the relationship between total vitamin D (vitamin D2 supplements plus endogenous vitamin D3) was a better predictor of complicat...

Background: Cardiac diseases are one of the major causes of death worldwide with increasing incidence rate per year, particularly in developing countries such as Sudan owing to urbanization and changing lifestyle. Myocardial infarction is a consequence of the imbalance between the heart blood supply and the required heart cell; this disorder leads...

Eumycetoma (mycotic mycetoma) is the fungal form of mycetoma, a subcutaneous infection occurring in individuals living in endemic areas of the disease. The Sudan is hyperendemic for mycetoma, with the highest incidence being reported from Gezira State, Central Sudan. The present study was conducted at the Gezira Mycetoma Center and aimed to determi...

Background:Chlamydia trachomatis (CT) is a sexually transmitted pathogen that threatens reproductive health worldwide. This study aims to screen CT urogenital infection using cytology and molecular methods in women suffering infertility. Methods: In total, 415 women suffering infertility, attending Wad Madani Maternity Hospital were included in thi...

Background: Human cytomegalovirus (HCMV) is a major cause of congenital infections. It is more widespread in developing countries and communities with low socioeconomic status. The infection can cause pregnancy loss or spontaneous abortion. Tests are available for the detection of HCMV IgG and IgM antibodies. Many pregnant women in Blue Nile State,...

Background: Toombak is known to be contain a high concentration of tobacco specific nitrose amine (TSNA), which is a potent carcinogen. Therefore, the high frequency of patients affected with oral cancer, who were mostly toombak dippers reflect the importance of the subject. In recent two decades, there are dramatic switch from histopathological to...

This paper reports a study of the LifeStraw in El-Masraf camp within Gezira State, Sudan. A total of 647 eligible subjects participated in the study. Two week incidence of diarrhoeal rates were estimated by a community survey some four months before and again four months after provision of the LifeStraw. In addition counts were kept of people atten...