Ecir Ali Çakmak

Sakarya University

Objective: Canine mammary gland tumors (CMGTs) are the most common tumors in female dogs and the main treatment options used in CMGTs are surgery caused some complications. Therefore, new treatment options are needed for the CMGTs. Traf2 and Nck-interacting serine protein kinase (TNIK) as a transcriptional coregulator of Wnt targeted genes is highl...

LncRNA dysregulation is known to be taking part in majority of cancers, including osteosarcoma. In one of our previous study, we showed that lncRNA MEG3 is being regulated by miR‐664a and suppresses the migratory potential of osteosarcoma cells (U‐2OS). We now report a novel lncRNA called ERICD is linked to the transcription factor ARID3A in U‐2OS...

Thiosemicarbazones (TSCs) are interesting group of chemical compounds that received significant levels of attention due their wide range of pharmacological effects including antibacterial, antiviral, and especially anti-tumor activities. Several thiosemicarbazone derivatives have been extensively reported recently with their anti-tumor properties b...

Pancreatic cancer is characterized by rapid metastasis and resistant to medical treatments. As the other cancers, mutations of tumor suppressor genes that involved in suppression of cell growth are observed in pancreatic cancers. ING4 protein is one of the proteins involved in the regulation of p53 tumor suppressor gene functions. ING4 involved in...

Akut promyelositik lösemi (APL), akut miyeloid lösemi (AML)’nin bir alt grubudur ve AML vakalarının %5-10’unu oluşturur. Farklılaşmaları promyelosit aşamasında bloke edilmiş hematopoietik öncül hücrelerin kemik iliğinde birikimi ile karakterizedir. mikroRNA’lar yaklaşık 19-25 nükleotid uzunluğunda, tek zincirli kodlanmayan RNA’lar olup, hedef mRNA’...

NOS3

EP300

Cancer is a consequence of accumulation of genetic and epigenetic alterations in the cell which can lead to activation of oncogenes or inactivation of tumor suppressor genes (TSG). Since members of ING family were discovered as TSGs in different cancer types, it was aimed to analyze the chromosome 13q33-34 region, ING1 and p53 genes in bladder canc...

MicroRNAs can regulate many biological functions. miR-122-5p has a tumor suppressor function through different molecular pathways. Also, our second hit, ADAM10, targeted by miR-122-5p, is a major determinant of HER2 shedding causing that trastuzumab cannot bind to HER2 receptors. Therefore, our analysis upon ADAM10 expression and miR-122-5p was a g...

Meme kanseri dünyada en yaygın görülen ikinci kanser türüdür. Bayanlarda daha sıklıkla görülmekle birlikte, tüm dünyada yılda ortalama 400.000 kişi ölmektedir. Meme kanserinde sağ kalım için erken teşhis ve tedavinin önemi büyüktür. Bu yüzden yeni tanı ve tedavi yaklaşımlarının araştırılması, geliştirilmesi büyük önem arz etmektedir. MikroRNA’lar,...

Cathepsinler lizozomal proteazlardır. Cathepsin B çeşitli patolojik ve onkojenik süreçlerde önemli rollere sahiptir. Çoğu malignant tümörlerde aşırı ekpsrese edildiği gösterilmiştir. Ayrıca Cathepsin B nin otofaji için gerekli olduğu belirtilmiştir. Cathepsin S tümör hücrelerinde invasyon, metastaz ve angiogenezisde rol oynamaktadır. Bu çalışmanın...

SIRT

Aim: Experimental and clinical studies showed that bikunin, a Kunitz-type protease inhibitor, found in urine and amniotic fluid has a role in spread of tumor cells by providing a significant reduction in the levels of urokinase-type plasminogen activator (uPA) and its specific receptor urokinase-type plasminogen activator receptor (uPAR). The aim...

Multiple Sclerosis

NF-kB2

The tubers of Bongardia chrysogonum, which are used in the folk medicine in the form of 2-3% decoction in the province of Gaziantep, are supposed to treat urinary track, prost-hate disorders, and hemorrhoid. The objective of this study was to assess the effect of these tubers on some serum parameters and spleen, liver, and kidney tissues in rats. O...

Beta (β)-thalassemia is the most frequently observed hereditary blood disorder in the world. It is characterized by deficiency of hemoglobin β-globin gene and is also a profoundly heterogeneous both at the molecular and clinical level. In the case of b-thalassemia, there is reduced (β+ type) or absent (βo type) synthesis of the beta chains of hemog...

Colorectal cancer (CRC) develops as a multi-step process which results from gradual accumulation of mutations in proto-oncogenes, tumor suppressor, and DNA repair genes. Mortality rate of CRC is very high. Therefore, development of alternative diagnostic methods which can be used in the early diagnosis is crucial. ATP2B4 gene encodes one of the fou...

Cardiovascular disease (CVD) risk factors, such as arterial hypertension, obesity, dyslipidemia or diabetes mellitus, as well as CVDs, including myocardial infarction, coronary artery disease or stroke, are the most prevalent diseases and account for the major causes of death worldwide. In the present study, 4,709 unrelated patients subjected to CV...

Familial mediterranean fever (FMF) is an autosomal recessive autoinflammatory disorder (MIM# 249100), particularly common in populations of Mediterranean extraction. MEFV gene, responsible for FMF, encoding pyrin has recently been mapped to chromosome 16p13.3. In the present study, 3,341 unrelated patients with the suspicion of FMF in south-east pa...

Extracts of Anthemis hyalina (Ah), Nigella sativa (Ns) and peels of Citrus sinensis (Cs) have been used as folk medicine to fight antimicrobial diseases. To evaluate the effect of extracts of Ah, Ns and Cs on the replication of coronavirus (CoV) and on the expression of TRP genes during coronavirus infection, HeLa-CEACAM1a (HeLa-epithelial carcinoe...

EP300

NOS3

Multiple skleroz

Multiple skleroz

SIRT

Cytokine-induced expression of suppressors of cytokine signalling (SOCS) molecules is important for the negative feedback control of STAT-dependent cytokine signalling. The aim of this study was to investigate possible association between the promoter region polymorphisms of the SOCS3 gene and metastatic colorectal carcinoma in a Turkish population...

ING4

P53

Diabetes is the leading cause of chronic renal failure. Our purpose was to determine the effects of N-nitro-l-arginine (l-NNA) and an extract of Stevia rebaudiana (Bertoni) (SrB) leaves on renal function in streptozotocin-nicotinamide (STZ-NA)-induced diabetic rats. Rats were divided into seven groups. Three of these groups were controls. Diabetes...

Fine-needle aspiration biopsy (FNA) is currently the best initial diagnostic test for evaluation of a thyroid nodule. FNA cytology cannot discriminate between benign and malignant thyroid nodules in up to 30% of thyroid nodules. Therefore, an adjunct to FNA is needed to clarify these lesions as benign or malignant. Using differential display-polyme...

Meningitis is an inflammatory disease caused by bacteria, fungi, and viruses with various clinical symptoms. Interleukin-10 (IL-10) levels have been shown to be increased in blood or cerebrospinal fluid of patients with meningitis, but the association of IL-10 gene promoter polymorphisms or gene expression with meningitis has not been evaluated. IL...

Meningitis is an inflammation of the protective membranes covering the brain and spinal cord caused by bacteria, fungi, or viruses with various clinical symptoms. Although meningitis is not so prevalent, it remains the most serious contagious disease. The aim of our study was to investigate the effect of gene expressions of nitric oxide synthases (...

Meningitis is an inflammatory disease caused by microorganisms and viruses with various clinical symptoms, which damages the brain tissue and membranes, and which induces biochemical and cellular changes in cerebrospinal fluid (CSF). Meningitis is an infectious disease with higher rates of mortality and morbidity. The possibility of death or perman...

Differential Display (DD), also known as DD-PCR or DDRT-PCR, is one of the major tools in interpreting gene expression patterns. With its simplicity, DD methodology also offers reproducibility, comparison of all mRNA species in the cell populations of interest, and isolation of corresponding cDNA. A number of different protocols have been evolved f...

Bikunin is an inhibitor of kidney stone formation synthesized in the liver together with α(1)-microglobulin from the α(1)-microglobulin/bikunin precursor (AMBP) gene. The aim of this study was to investigate the possible association between bikunin/AMBP gene polymorphisms and urinary stone formation. To analyse the DNA, blood samples were taken fro...

C-Kit

ING1

13Q33

The effect of occupational lead exposure on the liver function and on the blood biochemical parameters among the battery workers and the muffler repair workers was studied. The study included 22 battery and 38 muffler repair workers. Whole blood lead levels were determined by atomic absorption spectrophotometers. Total protein, albumin, globulin, c...

In an attempt to understand the impact of inhaled lead on the pulmonary functions, we assessed the blood lead levels and pulmonary functions of the battery and exhaust workers who are potential candidates for lead inhalation. The hospital staff served as control group. The measurements of lead levels were performed using atomic absorption spectroph...

Thirty patients with endometrioid adenocarcinomas of the endometrium who had undergone total abdominal hysterectomy and bilateral salpingo-oophorectomy were reviewed histopathologically. Tissues were stained immunohistochemically with p53 and bcl-2, respectively. Ten cases were selected as grade 1, ten cases grade 2 and ten cases grade 3. We observ...

Mutations in Connexin 26 (Cx26) play an important role in autosomal non-syndromic hereditary hearing loss. In this study, our objective was to find out the significance of Cx26 mutations in Turkish families who had hereditary deafness. Fourteen families who had at least two prelingually deaf children per family were included in the study. One affec...

The molecular biological analysis of infectious agents requires the availability of a reliable source of microorganisms to be used to recover DNA. Clinical samples can be obtained directly from infected patients or can be propagated using in vitro or in vivo systems. However, repeated sampling from patients is not always possible as the procedure m...

Antiepileptic drugs may cause the changes in the oxidant/antioxidant status of the body. Reactive oxygen species have been suggested to be an important factor in the pathogenesis of various diseaases as well as drug interactions and/or adverse effects. The aim of this study, therefore, is to investigate the status of major antioxidant enzyme activi...

Thirty cases with squamous carcinomas of cervix who underwent total abdominal hysterectomy and bilateral salphingo-oopherectomy were reviewed histopathologically. The tissues were stained immunohistologically with p53 and bcl-2. Ten cases were selected as well differentiated (large cell keratinizing) carcinoma, ten cases moderately differentiated (...

This report describes the first Turkish family to be diagnosed with Bernard-Soulier syndrome. The family consists of nine members (two parents, three sons and four daughters). The parents were first cousins. The index case, a 22 year-old-man, had a history of haemorrhagic diathesis with thrombocytopenia, giant platelets in the peripheral blood smea...

Evidence is accumulating for a possible role of nitric oxide (NO) in schizophrenia. Adrenomedullin (AM) induces vasorelaxation by activating adenylate cyclase and also by stimulating the release of NO. AM immune reactivity is present in the brain consistent with a role as neurotransmitter. We aimed to examine plasma levels of nitrite (a metabolite...

Background: Dehydroepiandrosterone sulfate (DHEAS) is the circulating form of the adrenal steroid dehydroepiandrosterone (DHEA). Both steroids have been associated with a variety of physiological processes in humans. DHEAS is the most abundant circulating adrenal steroid and shows a large range of normal variation; yet the precise physiological fun...

This study was intended to elucidate the diagnostic values of dermatoglyphic features on the 45 cerebral palsy (CP) patients (28 boys and 17 girls). There were 50 healthy children in the control group. Dermatoglyphic samples were obtained from the both groups by using the paper and ink method and than analysed. The types of dermal patterns of finge...

Seventy-eight non-invasive prostate specimens collected from patients with chronic non-bacterial prostatitis were evaluated by in situ hybridization (IH) for evidence of Chlamydia trachomatis. Intracellular Chlamydia bodies were detected in 18 of 78 cases (20.6%). Homogeneous blue-black bodies in the cellular cytoplasm were accepted as in situ posi...

The effect of ketotifen on ethanol-induced gastric lesions was examined in mice. For this purpose 36 mice were deprived of food for 20 hours. The mice were divided into four groups. Saline was given to the first group that is the control group by oesophageal intubation tube at first and then saline was given again after half an hour. Similarly, sal...

The effects of different doses of cyclosporin A (2.5, 10, 25, 50 mg/kg/day) on chromosomes and serum enzymes in rats were investigated. Cyclosporin A (all doses) had no numerical effect on chromosomes. There was a dose-dependent increase of chromatid-type breakage, serum SGOT, SGPT, ALP and ASP levels according to the control. These increases were...

Cyclosporine A (Cy A) in doses of 2.5, 10, 25 and 50 mg/kg/day were given intraperitoneally (i.p.) to rats for seven days. At the end of our study, no significant change had occurred in creatinine clearance and urine calcium levels in the experiment and control groups. Urine creatinine, sodium, potassium and urea nitrogen levels decreased while ser...

In the present study, ciprofloxacin, a fluoroquinolone antibiotic, was evaluated in vivo. The toxic effects of ciprofloxacin (20 and 200 mg/kg/day) on kidney and liver functions, and on chromosomes were investigated in rats. 20 mg/kg/day ciprofloxacin did not significantly affect urine composition, serum enzyme levels and the haematocrit, affecting...