Prathima Institute of Medical Sciences
Question
Asked 6th Jul, 2012
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Can anyone identify the genus of this bacteria?
It can produce blueish-green pigment in nutrient broth. Can utilize gelatin. Can produce H2S in SIM medium, urease positive, citrate and catalase positive. And it is also a phosphate solubilising bacteria, can produce IAA.
Most recent answer
You have not mentioned about oxidase pseudomonas are positive
Popular answers (1)
University of Georgia
I am surprised that so many suggested elaborate test when you can get for much less effort and money / time the definite answer using 16S rRNA sequence (Cost of sequence is about US $ 5 -29 , depending on the service, If the sequence is between two species you can do --based on Bergey's-- very few differentiating tests to nail it down. Don't forget you also could have a novel taxa and you don't find that out by just doing the suggested test.. Don't think in a box. -
41 Recommendations
All Answers (169)
Illinois Institute of Technology
You can always send a plate to SeqWright in Houston. For $100 they will identify it via 16S rRNA sequence.
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Helix Biological Laboratory in Michigan can do identifications via PCR and DNA sequencing of bacterial 16S rRNA for $80 dollars a sample with a relatively quick turn around time. Check them out at www.helixbiolab.com.
Southern Illinois University Carbondale
I would vote for Pseudomonas as well, in particular, P. aeruginosa. Are cells rod-shaped and motile (I assume you've looked at the culture under the microscope?)
2 Recommendations
Technical University of Lisbon
Pseudomonas aeruginosa or Pseudomonas fluorescens, depending on biochemical profile. Knowing where it was isolated from would help. Burkholderia cepacia is a less likely hypothesis.
Meenakshi Medical College Hospital and Research Institute,Kanchipuram,India
Only pseudomonas spp produce blue green pigment that to in an basic media like nutrient broth
Universidade Federal da Bahia
Yes, it is most likely to be a Pseudomonas (but Proteus may also produce some pigment).
2 Recommendations
Sakha Agricultural Research Station, Kafr El-Sheikh 33717, Egypt
I recommend the following site for help, good luck.
Yale University
Pseudomonas for sure. Try to observe the preparation under a Wood's Lamp to see if the pigment is fluorescent
New York University
Where was it isolated from?Infection/ enviromental sample? That information might greatly contribute to the right identification. As aforementioned, I also strongly recommend you DNA extraction and further sequencing of 16S rDNA amplicons. Identification based on sequencing helps to avoid misleading results associated with variable expression of some phenotypic traits depending on strain or cullture condition e.g. carbohydrate fermentation or other metabolic capabilities. Good luck!
Qassim University
yes i agree the last opinions especially with Mike Mitzel because this the right way to classify any strain. also you have to do one PCR run using the specific primer. Hence you will be sure that you have pseudomonase strain or what.....good luck
Sea6 Energy
ya this may be Pseudomonas sp if you want to confirm check in pseudomonas agar if it grows it wll confirm as Pseudomonas while checking see the fluoresence if it shows fluorescense it is confirm Pseudomonas sp
1 Recommendation
Colorado State University
Pseudonomas are all oxidase positive and motile. They all can oxidize glucose but not ferment it.
I suggest you send your plates to a contract lab for microbial identification, you may need to know the genetic aspects of all microorganisms identify or you can do it yourself by using biomerieux API kits or Remel.
Contract labs: MIDI or accugenix
Regards,
Suze Philistin
Università di Pisa
partial sequence of the 16S rDNA for genus, then genotyping comparing with typre strains
1 Recommendation
Boston Children's Hospital
i am quite sure it is Pseudomonas, and that bluish compound is pyocyanine a redox active compounds, and serve as quorum sensing molecule
Milliya Arts Science & Management Science College
on first look seems like Pseudomonas but should be confirmed
Ricerche Sperimentali Montale Pistoia
Probably Pseudomonas to confirm 16S rDNA ; you can send the strain at DSMZ Germany for the identification.
Indian Institute of Technology Kharagpur
You can get the genus by doing 16srDNA sequencing, then if you submit the sequence in NCBI blastn databases, it will give you the possible matches of the genus. like this by biochemical characterization you can't be confirmed 100%.
Corvinus University of Budapest
I also agree that 16S rDNA sequencing can solve your problem.The Pseudomonas genus is one of the most diverse genera. It is a bit tricky to identify Pseudomonas species based on biochemical tests and cultivation on different media. If you have possibility try to do the 16S rDNA sequencing (at least the parital sequencing).
1 Recommendation
Chargen Life Sciences (Biomall.in)
One thing I can tell you that P. aeruginosa is urease negative...so your culture might be some other species of Pseudomonas..You should check the family pseudomonadaceae in Bergey's Manual of systematic bacteriology and match your results of biochemical tests..
10 Recommendations
Indian Council of Medical Research
Mr. Dipak Paul
if you could send a stab culture or culture pellet in ethanol then I may try to identify both genus and species
2 Recommendations
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It is most likely to be Genus Pseudomonas and you can further authenticate the identification of this bacterium by 16S rDNA sequence amplification and FAME analysis.
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is it Pseudomonas lemonnieri??? check this out.http://www.ncbi.nlm.nih.gov/pmc/articles/PMC289768/pdf/jbacter00520-0029.pdf
3 Recommendations
Presidency University, Kolkata
What are its morphological features, both on plate & under the microscope?
Laboratorio de Salud Pública del Ayuntamiento de Madrid
Probably it is a specie of Genus pseudomona. Why don´t you test oxidase? If it is oxidase negative, then it is most likely to be Proteus and you can test for swarming in nutrient agar.
Milliya Arts Science & Management Science College
@ Ruhi sounds quite correct but needs verification with the isolate
Tabriz University of Medical Sciences
Mr.Dipak Paul first send the details about colony morphology, source of bacterium, gram's feature, oxidase, if gram negative, whether you have checked or confirmed with 3% KOH, then all biochemical tests performed.
send details to guess the bacteria Thanks
University of Granada
Fam Pseudomonaceae: Gram - oxidasa + catalase +
Gen Pseudomona: gelatinasa + ureasa +
Pseudomona aeruginosa: (Bacilo piocianic) Can reduce NO3- to NO2-
High resistance. (Use polimixine- Gentamicine).
1 Recommendation
University of Rwanda
What is the Gram reaction and what is the shape of the bacteria when seen under the microscope? Not all green pigment producing bacteria are Pseudomonas sp
1 Recommendation
Canterbury Health Laboratories
One quick way of identfication would be to run teh bacterial colony though a MALDI-TOF MS.
University of Sciences, Techniques and Technology of Bamako
If it is possible to have a sample of this bacteria, we have a Biolog system to identify it to you. We can also identify it by the DNA sequencing.
Best regards
National Institute of Health Dr. Ricardo Jorge
16S rDNA sequencing should give a definitive identification. That would be my choice. Good luck!
2 Recommendations
Sea6 Energy
Dipak you just go for FAME analysis once you got the result you just compare with 16S rDNA sequencing data it probably will give the better result of your bacteria.........
Kuwait University
If it is gram negative rod polarly flagelated bacteria, it might be a Pseudomans species???
Prathima Institute of Medical Sciences
I am late but genus might be Peudomonas pls check in COWAN and STEEL identification of bacteria manual
1 Recommendation
University of Stirling
Is the pigment fluorescent? What is the source of the culture - I have seen similar organisms in marine samples.
1 Recommendation
Kemin Industries
grow this culture in argenine proline broth with ethanol if growth occur then it is Psedomonas aeruginosa................
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If Gram negative, its probably Pseudomonas as I have already replied earlier. If Gram positive, then you might be just dealing with an actinomycete. Since the sample is riverine, these are the two best options I can suggest. However, you cannot negate the possibility of serratia, but in general they are known to produce pigments of the red-orange-pink group.. all the best.
1 Recommendation
University of Oxford
16s korechis???tahole e to jante parbi ki genus...species ta ektu chaper bapar...otr jonno onek onek khatte hobe.. :-)
Pt. Jawahar Lal Nehru Memorial Medical College
It looks like Pseudomonas but if want to identify up-to species level better go for sequencing.
Anand Agricultural University
Dear you are talking about it's genus then 99% it might belong to Pseudomonas sp.
Sant Gadge Baba Amravati University
Properties so far suggest it is one of the Pseudomonas species. Sill 16S rRNA sequencing may clear the facts.
Roundglass Foundation
It coud also be Azospirillum.... once got similar thing when isolating Azospirillum lipoferm from paddy fields.....
Medical Research Council Unit, The Gambia Unit
What is the oxidase response? Does it ferment lactose? I'm also inclined to the family pseudomonadaceae; Oxidase should test positive to this.
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The genus might be Pseudomonas. Moreover, you need to perform biochemical tests and molecular characterization (16S rDNA sequence analysis) of your organism for authentification of aforesaid genus.
2 Recommendations
This bacterium is certainly Pseudomonas,Melt gelatin tests, the sugar trehalose, growth at temperatures of 41 and 4 ° C. is major indexes for Separation of the Pseudomonas species, But I think it is Pseudomonas aeruginosa.
1 Recommendation
Indian Council of Medical Research
A few points to note.Some strains of Pseudomonas aeruginosa can be urease positive.However, definitely NOT H2S producers..
Claude Bernard University Lyon 1
Rod ? cocci ? Gram +, Gram - . These microcscopic characteristics would be very useful here.
1 Recommendation
Universidad de Buenos Aires, Buenos Aires; Argentina
It's a rod G- Oxidase -? You need to check first this point!
University of Hertfordshire
you need to follow a systematic approach to the identification, colony morphology, microscopic morphology, gram stain, primary/secondary biochemical tests. Then followed by the specific genus/species tests. This would be the standard approach that all science student are shown in year one. Use a good book, eg Bergies Manual to perform the identification. Unless you do this your isolate could be anything.
1 Recommendation
European Molecular Biology Laboratory
I also think you should use a systemaytic approach for the identification, or if you can you could go for a 16S seqeunce whcih gives you really good informations. But I think that this bacterium is close related to some Pseudomonas species. The coloration could be due to phenazine production, check this paper http://www.nature.com/nchembio/journal/v2/n2/abs/nchembio764.html
Best
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What are you growing the organism in? You would get the same colour if indole was converted to idigo by a dioxygenase
SRM Institute of Science and Technology - Ramapuram
as per your biochemical test it may be Pseudomonas..better your try to sub culture it on Kings B agar which is selective medium for Pseudomonas. and try with Cetramide agar too..for better confirmation 16S rRNA sequencing is best option
The California Institute for Biomedical Research
I am not answering the question, but I wish to comment on the right use of the word bacteria. The pharse "this bacteria" should read "this bacterium".
5 Recommendations
National University of Ireland, Maynooth
I'd say just amplify the 16S rRNA genes and sequence them. Nadkarni et al 2002 describe the primers and conditions needed.
2 Recommendations
University of Florida
Hi Dipak, thanks for posting this question. Your bug appears to be a Pseudomonas to me also.... though a confirmatory test would be better. Depending on your time and available funds you can try to identify your bacterium. If you have funds for a 16S rRNA analysis, please go ahead and do that (you can reach the IISER in kalyani; IICB/ICGEB/IMTECH/CCMB/NCL/NIO/IITKGP/TIFR/ and many more institutes in India itself who can do it for you in less than 3k INR). If you have time but lack of funds, please grab the Bible for microbiology (Bergey's manual ), carry out some (may be lots too) biochemical tests, and you should be done with the identification unless you have a new bug that would be very difficult to funnel out from those tests and charts... Good luck...:-)
Built Water Solutions
The pigment looks like pyacyanin which would mean you have a urease positive strain of P.aeruginosa!
University of Georgia
I am surprised that so many suggested elaborate test when you can get for much less effort and money / time the definite answer using 16S rRNA sequence (Cost of sequence is about US $ 5 -29 , depending on the service, If the sequence is between two species you can do --based on Bergey's-- very few differentiating tests to nail it down. Don't forget you also could have a novel taxa and you don't find that out by just doing the suggested test.. Don't think in a box. -
41 Recommendations
Swami Ramanand Teerth Marathwada University
yes i also agree with Sir Wiegel, you have to go for 16S rRNA sequencing, then you can easily determine its phylogenetic related species.
Karpagam Academy of Higher Education
hi
It may be Pseudomonas.fluorescence .Because it got the ability to produce both pyoverdin and pyocyanin ,And this microbes got little effect on phosphorus soliubilization around (0.6-0.8 ) PPM. For more details go for inhibition tests as it is effective against rice sheath rot disease .....All the best
Feel free to contact me
Abiram
Zagazig University
p. aeruginosa confirm on cetrimide medium it is specific for it. always use Bergyes manual
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