David Wibowo

Thermo Fisher Scientific | TFS · Operations - Downstream Processing

There is an unmet need for antigen delivery systems that elicit efficient T cell priming to prevent infectious diseases or for treatment of cancers. Here, we explored the immunogenic potential of biologically assembled biopolymer particles (BPs) that have been bioengineered to display the antigenic MHC I and MHC II epitopes of model antigen ovalbum...

Electrohydrodynamic atomization has been emerging as a powerful approach for respiratory treatment, including the generation and delivery of micro/nanoparticles as carriers for drugs and antigens. In this work, we present a new conceptual design in which two nozzles facilitate dual electrospray coexisting with ionic wind at chamfered tips by a dire...

Subunit vaccines are more advantageous than live attenuated vaccines in terms of safety and scale-up manufacture. However, this often comes as a trade-off to their efficacy. Over the years, polymeric nanoparticles have been developed to improve vaccine potency, by engineering their physicochemical properties to incorporate multiple immunological cu...

Pseudomonas aeruginosa is an opportunistic human pathogen causing devastating acute and chronic infections in individuals with compromised immune systems. Its highly notorious persistence in clinical settings is attributed to its ability to form antibiotic-resistant biofilms. Biofilm is an architecture built mostly by autogenic extracellular polyme...

Cadmium (Cd)-contaminated waterbodies are a worldwide concern for the environment, impacting human health. To address the need for efficient, sustainable and cost-effective remediation measures, we developed innovative Cd bioremediation agents by engineering Escherichia coli to assemble poly(3-hydroxybutyric acid) (PHB) beads densely coated with Cd...

Droplet microfluidics creates new opportunities for microbial engineering. Most microbial cultivation are carried out in bioreactors which are usually bulky and consume large amount of reagents and media. In this paper, we propose a microfluidic droplet-based microbioreactor for microbial cultivation. A microfluidic device was designed and fabricat...

Biomineralization of metal organic frameworks (MOFs) using biomolecules has recently attracted significant interest because of the benign process including room temperature, neutral pH and without the requirement of any other chemical reagents. Also, these biomolecule incorporated MOFs ([email protected]) have demonstrated their potential in biomol...

The ability of cells to sense external mechanical cues is essential for their adaptation to the surrounding microenvironment. However, how nanoparticle mechanical properties affect cell-nanoparticle interactions remains largely unknown. Here, we synthesized a library of silica nanocapsules (SNCs) with a wide range of elasticity (Young’s modulus ran...

Encapsulation of biomoleucles in metal organic frameworks (MOFs) has recently attracted significant interest because of the benign process including room temperature, neutral pH and without the requirement of any other chemical reagents. Also, these biomolecule incorporated MOFs (biomolecules@MOFs) have demonstrated their potential in biomolecule p...

Document recommendation systems for locating relevant literature have mostly relied on methods developed a decade ago. This is largely due to the lack of a large offline gold-standard benchmark of relevant documents that cover a variety of research fields such that newly developed literature search techniques can be compared, improved and translate...

Document recommendation systems for locating relevant literature have mostly relied on methods developed a decade ago. This is largely due to the lack of a large offline gold-standard benchmark of relevant documents that cover a variety of research fields such that newly developed literature search techniques can be compared, improved and translate...

Various pesticide nanocarriers have been developed. However, their pest-control applications remain limited in laboratories. Herein, we developed silica nanocapsules encapsulating fipronil (SNC) and their engineered form, poly(ethyleneimine)-coated SNC (SNC-PEI), based on recombinant catalytic modular protein D4S2 and used them against termite colo...

Kleintransporter auf der Nanoskala: Bifunktionale amphiphile Peptide wurden entwickelt, die sowohl hydrophobe Wirkstoffnanopartikel stabilisieren als auch Bio‐Verkieselung an der Partikeloberfläche verursachen können, wodurch die Langzeitstabilität erhalten bleibt. Die Wirkstoff‐Kern‐/Siliciumdioxid‐Schalen‐Nanokomposite zeigen eine außergewöhnlich...

The physicochemical properties of nanoparticles play critical roles in regulating nano-bio interactions. While the effects of the size, shape and surface charge of nanoparticles on their biological performances have been extensively investigated, roles of nanoparticle mechanical properties in drug delivery which has only been recognized recently re...

A large range of nanoparticles have been developed to encapsulate hydrophobic drugs. However, drug loading is usually less than 10 % or even 1 %. Now, core–shell nanoparticles are fabricated having exceptionally high drug loading up to 65 % (drug weight/the total weight of drug‐loaded nanoparticles) and high encapsulation efficiencies (>99 %) based...

Spherical polyhydroxyalkanoate (PHA) inclusions are naturally self-assembled inside bacteria. These PHA beads are shell-core structures composed of a hydrophobic PHA core surrounded by proteins, such as the PHA synthase (PhaC). PhaC is covalently attached and served as anchor protein for foreign protein such vaccine candidate antigens. PHA beads di...

Antibiotic resistance poses a growing threat to global public health. It is urgent to develop new alternative antibiotics. Antimicrobial peptide (AMP) is a diverse class of natural-occurring molecules that constitute immune systems of living organisms. More than 2500 AMPs have been identified and isolated from natural sources. Compared to conventio...

The adsorption behavior of activated carbons is determined not only by their porous structures but also by the chemical nature of its surface. The surface chemistry of activated carbons can be selectively modified in order to improve their adsorption capacity. In this study, a NORIT granular activated carbon was treated by oxidant (HNO3) and non-ox...

In recent years, antimicrobial peptides (AMPs) have attracted increasing attention. The microbial cells provide a simple, cost-effective platform to produce AMPs in industrial quantities. While AMP production as fusion proteins in microorganisms is commonly used, the recovery of AMPs necessitates the use of expensive proteases and extra purificatio...

Precise engineering of nanoparticles with systematically varied properties (size, charge surface properties, targeting ligands, etc.) remains a challenge, limiting the effective optimization of nanoparticles for particular applications. Herein we report a single-step microfluidic combinatorial approach for producing a library of single and dual-lig...

The concept of dual‐ligand targeting has been around for quite some time, but remains controversial due to the intricate interplay between so many different factors such as the choice of dual ligands, their densities, ratios and length matching, etc. Herein, the synthesis of a combinatorial library of single and dual‐ligand nanoparticles with syste...

The physicochemical properties of nanoparticles (size, charge and surface chemistry, etc.) influence their biological functions often in complex and poorly understood ways. This complexity is compounded when the nanostructures involved have variable mechanical properties. Here, we report the synthesis of liquid-filled silica nanocapsules (SNCs, ~ 1...

Antimicrobial peptides (AMPs) have significant potential as alternatives to classical antibiotics. However, AMPs are currently prepared using processes which are often laborious, expensive and of low-yield, thus hindering their research and application. Large-scale methods for production of AMPs using a cost-effective approach is urgently required....

Designed peptide surfactants offer a number of advanced properties over conventional petrochemical surfactants, including biocompatibility, sustainability, and tailorability of the chemical and physical properties through peptide design. Their biocompatibility and degradability make them attractive for various applications, particularly for food an...

This paper reports the dynamic interfacial behavior of a new interfacially-active peptide AM-S which was designed based on a peptide surfactant AM1 modularized with an additional silk-derived hydrophobic tail to enhance anchoring to air–water interfaces. AM-S peptide shows a random coil conformation in bulk solution similar to AM1 as determined by...

Silica nanocapsules have attracted tremendous interest for encapsulation, protection and controlled release of various cargoes due to their unique hierarchical core-shell structure. However, it remains challenging to synthesize silica nanocapsules having high cargo-loading capacity and cargo-protection capability without compromising process simpli...

Multiphase microfluidics has attracted significant interest in making micro- and nanostructures for various applications because of its capabilities in precisely controlling and manipulating a small volume of liquids. In this review, we introduce the recent advances in making micro- and nanostructures for pharmaceutical applications, including micr...

Anaplasma marginale is a devastating tick-borne pathogen causing anaplasmosis in cattle and results in significant economic loss to the cattle industry worldwide. Currently, there is no widely accepted vaccine against A. marginale. New generation subunit vaccines against A. marginale, which are much safer, more efficient and cost-effective, are in...

Silica nanocapsules have attracted significant interest due to their core-shell hierarchical structure. The core domain allows the encapsulation of various functional components such as drugs, fluorescent and magnetic nanoparticles for applications in drug delivery, imaging and sensing, and the silica shell with its unique properties including bioc...

Inspired by Nature, synthetic mineralizing proteins have been developed to synthesize various structures of silica-based nanomaterials under environmentally-friendly conditions. However, the development of bioprocesses able to assist in the translation of these new materials has lagged the development of the materials themselves. The development of...

We recently developed a novel approach for making oil-core silica-shell nanocapsules using designed bifunctional peptides (also called biomineralizing peptide surfactants) having both surface activity and biomineralization activity. Using the bifunctional peptides, oil-in-water nanoemulsion templates can be readily prepared, followed by the silicif...

Silica nanocapsules having core–shell architecture are of great interest in many current and emerging areas of technology. The core provides high-capacity loading of various actives, while the silica shell serves as protective envelope and diffusion barrier enabling controlled release of the active. Currently, production of silica nanocapsules is m...

Interfacially-driven synthesis of oil-core silica-shell nanocapsules using a rationally-designed Recombinant Catalytic Modular Protein (ReCaMoP), in lieu of a conventional chemical surfactant, is reported. A 116-residue protein, D4S2, was designed by modularizing a surface-active protein module having four-helix bundle structure in bulk and a biosi...

A pesticide delivery system made of biocompatible components and having sustained release properties is highly desirable for agricultural applications. In this study, we report a new biocompatible oil-core silica-shell nanocapsule for sustained release of fipronil insecticide. Silica nanocapsules were prepared by a recently-reported emulsion and bi...

A novel, bio-inspired templating platform technology is reported for the synthesis of biocompatible oil-core silica-shell nanocapsules with tunable shell thickness by utilizing a designed bifunctional peptide. Furthermore, facile encapsulation of an active molecule and its sustained release are demonstrated.

Quaternary ammonium containing compounds (QACs) such as cetylpyridinium chloride (CPC) is commonly employed in hyaluronic acid (HA) production process as an HA precipitating agent. 3-(Trimethoxysilyl)-propyldimethyloctadecyl ammonium chloride, a Si containing QAC (Si-QAC) generally used to modify the surface of cotton fibers for the preparation of...

The influence of surface chemistry and solution pH on the adsorption of benzene and toluene on activated carbon and its acid and heat treated forms were studied. A commercial coal-based activated carbon F-400 was chosen as carbon parent. The carbon samples were obtained by modification of F-400 by means of chemical treatment with HNO3 and thermal t...