David Bauer

Tulane University | TU · Department of Microbiology and Immunology

Although most pathogens infect the human body via mucosal surfaces, very few injectable vaccines can specifically target immune cells to these tissues where their effector functions would be most desirable. We have previously shown that certain adjuvants can program vaccine-specific helper T cells to migrate to the gut, even when the vaccine is del...

Adjuvants are often essential additions to vaccines that enhance the activation of innate immune cells, leading to more potent and protective T and B cell responses. Only a few vaccine adjuvants are currently used in approved vaccine formulations in the United States. Combinations of one or more adjuvants have the potential to increase the efficacy...

COVID-19 has had an unprecedented global impact on human health. Understanding the Ab memory responses to infection is one tool needed to effectively control the pandemic. Among 173 outpatients who had virologically confirmed SARS-CoV-2 infection, we evaluated serum Ab concentrations, microneutralization activity, and enumerated SARS-CoV-2-specific...

Enterotoxigenic Escherichia coli (ETEC) remain a major cause of diarrheal mortality and morbidity in children in low-resource settings. Few studies have explored the consequences of simultaneous intoxication with heat-stable (ST) and heat-labile (LT) enterotoxins despite the increased prevalence of wild ETEC isolates expressing both toxins. We ther...

COVID-19 has had an unprecedented global impact on human health. Understanding the antibody memory responses to infection is one tool needed to effectively control the pandemic. Among 173 outpatients who had virologically confirmed SARS-CoV-2 infection, we evaluated serum antibody concentrations, microneutralization activity, and enumerated SARS-Co...

Non-typhoidal salmonellosis, caused by Salmonella enterica serovar Typhimurium is a common fecal-oral disease characterized by mild gastrointestinal distress resulting in diarrhea, chills, fever, abdominal cramps, head and body aches, nausea, and vomiting. Increasing incidences of antibiotic resistant invasive non-typhoidal Salmonella infections ma...

Mast cells are potent mediators of allergy and asthma, yet their role in regulating adaptive immunity remains ambiguous. On the surface of mast cells, the crosslinking of IgE bound to FcεRI by a specific antigen recognized by that IgE triggers the release of immune mediators such as histamine and cytokines capable of activating other immune cells;...

The initiation of innate immune responses is critical for the formation of a protective adaptive immune response following vaccination. Innate immune cells, such as dendritic cells (DCs) and macrophages, phagocytose and display antigen, produce immune modulators and migrate to lymphoid tissues, leading to the activation of naïve T cells. These prof...

Differences in the immune response between the sexes, particularly in the susceptibility to and severity of infectious diseases, are well established. Females commonly generate a more robust response that is beneficial when combatting infections but leads to an increased risk of developing autoimmune disorders. While the sex-related hormone, estrad...

Human tumors with exonuclease domain mutations in the gene encoding DNA polymerase ε (POLE) have incredibly high mutation burdens. These errors arise in four unique mutation signatures occurring in different relative amounts, the etiologies of which remain poorly understood. We used CRISPR-Cas9 to engineer human cell lines expressing POLE tumor var...

The intracellular bacterial pathogen Salmonella is able to evade the immune system and persist within the host. In some cases, these persistent infections are asymptomatic for long periods and represent a significant public health hazard as potential chronic carriers, yet the mechanisms that control persistence are incompletely understood. Using a...

Mast cells are important immune mediators located at host environment interfaces and are most well-known as the major mediators of allergy and asthma. When activated, they have the ability to release prestored granules that contain immune modulating compounds, such as histamine and inflammatory cytokines. In skin, mast cell activation happens via c...

Non-typhoidal salmonellosis, caused by Salmonella enterica serovar Typhimurium (S. Typhimurium) is a common disease worldwide that can be contracted from contact with contaminated food, water, or animal carriers. Salmonellosis is characterized by mild gastrointestinal distress resulting in diarrhea, chills, fever, abdominal cramps, head and body ac...

Adjuvants are important mediators of immune responses and enhance the activation of innate immune cells, leading to protective T and B cell responses. Only a few vaccine adjuvants are currently approved for use in the US and, along with the discovery of novel adjuvants, combination adjuvants are likely to have a major impact on future vaccine devel...

The existence of chronic typhoid carriers has been in the public eye for over 100 years in part because of the publicity around Typhoid Mary. Additionally, it has been known for decades that the gallbladder is the main site of persistence and recently that gallstones play a key role. Despite this, very little is known about the physiological condit...

Enterotoxigenic Escherichia coli (ETEC) is a major cause of infectious diarrhea in children, travelers and deployed military personnel. As such, development of a vaccine would be advantageous for public health. One strategy is to use subunits of colonization factors combined with antigen/adjuvant toxoids as an ETEC vaccine. Here, we investigated in...

One option for achieving global polio eradication is to replace the oral poliovirus vaccine (OPV), which has the risk of reversion to wild-type virulence, with the inactivated poliovirus vaccine (IPV) vaccine. Adjuvants and alternate routes of immunization are promising options that may reduce antigen dose in IPV vaccinations, potentially allowing...

Enterotoxigenic E. coli (ETEC) are a significant cause of diarrheal disease and death, especially in children in developing countries. ETEC cause disease by colonizing the small intestine and producing heat-labile toxin (LT), heat-stable toxin (ST), or both LT and ST (LT/ST). The majority of ETEC produce both ST and LT. Despite the prevalence of LT...