Daniel Noyola

Universidad Autónoma de San Luis Potosí | UASLP · Facultad de Medicina

Respiratory syncytial virus (RSV), a leading cause of lower respiratory tract infections, is classified in two major groups (A and B) with multiple genotypes within them. Continuous changes in spatiotemporal distribution of RSV genotypes have been recorded since the identification of this virus. However, there are no established criteria for genoty...

Background: Respiratory syncytial virus (RSV) is the leading cause of severe acute respiratory infections (ARI) in preterm infants. The incidence of RSV-associated hospitalizations has not been defined in Mexico. Objectives: To determine the incidence of ARI- and RSV-associated hospitalizations in preterm infants during the first year of life....

In 2022, several countries reported an increase in respiratory syncytial virus (RSV) infections. We assessed the clinical characteristics and outcomes of infants hospitalized with RSV and compared them with infants hospitalized between 2009 and 2015. No significant differences in underlying disorders, intensive care unit admission rates and mortali...

During the COVID-19 pandemic, nonpharmaceutical interventions (NPIs) were implemented in order to control the transmission of SARS-CoV-2, potentially affecting the prevalence of respiratory syncytial virus (RSV). This review evaluated the impact of NPIs on RSV-related hospitalizations in children during the lockdown (2020–2021) compared to the pre-...

Background Population-based information regarding the impact of respiratory syncytial virus (RSV) and influenza on hospital admissions and mortality is scant for many countries. Methods Prospective testing of RSV and influenza virus was undertaken in patients <5 years old admitted to hospital with acute respiratory infection (ARI) between July, 20...

Mumps is an acute, contagious viral illness. Parotid gland swelling is the typical clinical manifestation, but many organ systems may be affected. Hearing loss (HL) is rare but a significant complication of the disease. Since it is vaccine-preventable, childhood mumps has become an uncommon disease in countries where measles-mumps-rubella (MMR) vac...

Human respiratory syncytial virus (hRSV) is the leading cause of acute lower respiratory tract infections in children under five years of age and older adults worldwide. During hRSV infection, host cells undergo changes in endomembrane organelles, including mitochondria. This organelle is responsible for energy production in the cell and plays an i...

Background Human Rhinoviruses (HRV) are a common cause of influenza-like illness, with the ability to infect the upper and lower respiratory tracts. In this study we aim to describe the clinical and molecular features of HRV infection in Mexican children and adults. Methods We performed a hospital-based, 4-year multicenter prospective observationa...

Before the current pandemic, influenza and respiratory syncytial virus (RSV) were the leading etiological agents of seasonal acute respiratory infections (ARI) around the world. In this setting, medical doctors typically based the diagnosis of ARI on patients’ symptoms alone and did not routinely conduct virological tests necessary to identify indi...

Human metapneumovirus (HMPV) is an important respiratory pathogen and is divided in two main groups (A and B). HMPV strains with partial duplications (111-nt and 180-nt duplication) of the G gene have been reported in recent years. Since the initial reports, viruses with these characteristics have been reported in several countries. We analyzed all...

Cytomegalovirus infection occurs commonly during infancy. Postnatal infection in term infants is usually asymptomatic; however, infection in preterm infants can be associated with clinical manifestations during the neonatal period. Nevertheless, few studies to assess the frequency of cytomegalovirus infection in preterm infants have been performed...

Introduction In the paediatric population, coronavirus disease (COVID-19) is usually asymptomatic or mild, but there are also severe and fatal cases. Methods We analysed data on COVID-19 cases from the national and state-level databases of the Federal Ministry of Health of Mexico and the Department of Health of Mexico City to determine the clinica...

Respiratory syncytial virus (RSV) is the main cause of severe respiratory infections in young children. The need for global epidemiologic data regarding RSV has been increasingly recognized. RSV A infections are reported more frequently than RSV B. Nonetheless, the temporal distribution of infections caused by both RSV groups has not been investiga...

Objective: Influenza is a costly disease for the population. It is a cause of seasonal morbidity and mortality, epidemics and pandemics or syndemics. Given the variability of the virus, surveillance systems are implemented in order to update the strains and include them in the annual influenza vaccine. This vaccine is currently recommended in some...

Introduction: Citrobacter spp. is an opportunistic bacteria that have been recognized as significant pathogens in patients with underlying diseases or immunocompromised status. The aim of this study was to identify extended-spectrum β-lactamases in clinical isolates of Citrobacter spp. Methods: This cross-sectional study was conducted at Hospita...

Introduction: Mortality rates associated with COVID-19 vary widely between countries and, within countries, between regions. These differences might be explained by population susceptibility, environmental factors, transmission dynamics, containment strategies, and diagnostic approaches. We aimed to analyze if obesity and diabetes prevalence are a...

La influenza es una enfermedad costosa para la población. Es causa de morbimortalidad estacional, epidemias y pandemias o sindemias. Debido a la variabilidad del virus, se implementan sistemas de vigilancia para actualizar las cepas e incluirlas en la vacuna antiinfluenza anual. Actualmente se recomienda esta vacuna en algunos grupos de alto riesgo...

Background: There has been a global increase in the prevalence of obesity in pregnant women in recent years. Animal studies have shown that intrauterine environment associated with maternal obesity leads to epigenetic changes. However, the effects of epigenetic changes occurring before birth in response to maternal conditions have not been clearly...

Background Respiratory syncytial virus (RSV) is a leading cause of pediatric death, with >99% of mortality occurring in low- and lower middle-income countries. At least half of RSV-related deaths are estimated to occur in the community, but clinical characteristics of this group of children remain poorly characterized. Methods The RSV Global Onlin...

RESUMEN Introducción: En la población pediátrica, el COVID-19 suele ser asintomático o leve, pero puede haber casos graves y mortales. Métodos: Se analizaron datos de los casos de COVID-19 registrados en las bases de datos nacional y regional de la Secretaría de Salud federal de México y la Secretaría de Salud de Ciudad de México para establecer la...

Background Influenza is a major cause of mortality worldwide. Most influenza-associated deaths are associated with cardiovascular or respiratory disorders. However, a large proportion of influenza-associated deaths do not have respiratory or cardiovascular disorders declared as the underlying cause of death. Diabetic individuals are at increased ri...

Respiratory syncytial virus (RSV) is a major cause of respiratory infections and is classified in two main groups, RSV-A and RSV-B, with multiple genotypes within each of them. For RSV-B, more than 30 genotypes have been described, without consensus on their definition. The lack of genotype assignation criteria has a direct impact on viral evolutio...

Background: Staphylococcus aureus is a leading cause of broad-spectrum infections both in the community and within healthcare settings. Methicillin-resistant Staphylococcus aureus (MRSA) has become a global public health issue. The aim of this study was to examine the clinical and molecular characteristics of Staphylococcus aureus isolates and to...

The study of infectious disease behavior has been a scientific concern for many years as early identification of outbreaks provides great advantages including timely implementation of public health measures to limit the spread of an epidemic. We propose a methodology that merges the predictions of (i) a computational model with machine learning, (i...

The Human Immunodeficiency Virus (HIV) Viral Infectivity Factor (Vif) is a 192-amino acid accessory protein essential to viral replication which counteracts host APOBEC3 proteins. APOBEC3 proteins interfere with the replication of HIV, hepatitis C virus, hepatitis B virus and retrotransposons. Vif is a recent candidate target for therapeutic and pr...

Background Nosocomial infections are a leading cause of morbidity, costs and mortality in preterm newborns. Most reports regarding nosocomial infections in neonatal intensive care units (NICU) are focused on bacterial infections and there is limited information regarding the impact of nosocomial viruses. The objective of this study was to assess th...

Protease is one of three enzymes encoded within HIV's pol gene and a retroviral aspartyl-protease which unlike other aspartyl-proteases form a 22kDa homodimer to exert its activity. Protease is responsible for the cleavage of viral Gag-Pol polypeptide into mature viral proteins and a target of current anti-retroviral therapy (ART). Saquinavir was t...

Background Molecular detection methods allow for the simultaneous detection of several infectious agents. This study assesses whether co-infection with two viruses as compared to one is associated with increased hospitalization in those with acute respiratory infections. Methods We prospectively enrolled a cohort of pediatric and adult participant...

Protease is one of three enzymes encoded within HIV's pol gene, responsible for the cleavage of viral Gag-Pol polypeptide into mature viral proteins and a target of current anti-retroviral therapy. Protease diversity analysis in Latin America has been lacking in spite of extensive studies of protease-inhibitor resistance mutations. We studied the d...

BACKGROUND: Influenza virus, respiratory syncytial virus, parainfluenza virus, and metapneumovirus are the most common viruses associated with acute lower respiratory infections in young children (<5 years) and older people (≥65 years). A global report of the monthly activity of these viruses is needed to inform public health strategies and program...

Background: Lower respiratory tract infections (LRTI) are one of the most common causes of death worldwide. Respiratory syncytial virus (RSV) is a leading cause of LRTI in children. Despite of its epidemiological importance, there is limited information regarding the impact of this virus in Latin America. Aims of the study: We carried out a pros...

Human Parainfluenza viruses (HPIV) type 1 and 3 are important causes of respiratory tract infections in young children globally. HPIV infections do not confer complete protective immunity so reinfections occur throughout life. Since no effective vaccine is available for the two virus subtypes, comprehensive understanding of HPIV-1 and HPIV-3 geneti...

Influenza and respiratory syncytial virus (RSV) are the leading etiological agents of seasonal acute respiratory infections (ARI) around the world. Medical doctors typically base the diagnosis of ARI on patients' symptoms alone and do not always conduct virological tests necessary to identify individual viruses, which limits the ability to study th...

Influenza is a leading cause of respiratory tract infections worldwide and there is limited information on the impact of the influenza A(H1N1)pdm virus on mortality after the 2009 pandemic. Using national mortality register data through 1998–2015 in Mexico, influenza-associated mortality was estimated for respiratory, cardiovascular, and all-cause...

Leukocyte immunoglobulin like receptor B1 (LILRB1) is a receptor with a significant role in several infectious, autoimmune, cardiovascular, and oncologic disorders. LILRB1 expression varies between individuals and may be associated to polymorphisms on the regulatory region of the LILRB1 gene, as well as to previous cytomegalovirus infection. We ana...

Background Acute respiratory infections are a major cause of morbidity in children, and are often caused by viruses. However, the relative severity of illness associated with different viruses is unclear. The objective of this study was to evaluate the risk of hospitalization from different viruses in children presenting with an influenza like illn...

This study set out to determine the frequency of antiretroviral drug resistance mutations in treatment-naïve subjects of the north central Mexican state of San Luis Potosí. Mexican studies of antiretroviral drug resistance mutations have focused mainly on large metropolitan areas and border towns subjected to intense international migrations. This...

We found that respiratory syncytial virus (RSV) ON1 genotype circulates in Mexico at least since November of 2009. Amino acid sequence analysis of RSV strains from different countries suggests that ON1 strains resulted from at least three independent duplication events. ABSTRACT Background: Respiratory syncytial virus (RSV) is a leading cause of re...

Background: Respiratory syncytial virus (RSV) is a leading cause of respiratory infections. An RSV-A genotype (ON1) that contains a 72-nt duplication was reported in 2012 and has extended worldwide since. Methods: We analyzed 345 respiratory samples obtained between 2003 and 2014 to assess the relevance of ON1 infections. Nucleotidic and deduced...

Influenza and respiratory syncytial virus are the leading etiologic agents of seasonal acute respiratory infections around the world. Medical doctors usually base the diagnosis of acute respiratory infections on patients' symptoms and do not always conduct laboratory tests necessary to identify individual viruses due to cost constraints. This limit...

Background: The perinatal environment has a role in the establishment of altered metabolic and inflammatory responses, and could be modulated by microRNAs regulating immune and metabolic processes. Objective: To analyze the expression profile of four circulating microRNAs and cytokine serum concentrations in neonates born to overweight and obese...

Introduction: Acute respiratory infections are the leading cause of mortality in children worldwide, especially in developing countries. Pneumonia accounts for 16% of all deaths of children under 5 years old, killing 935,000 children in 2015. Despite its frequency and severity the information about its etiology is limited. The aim of the study was...

Background: Influenza is a leading cause of respiratory tract infections among children. In Mexico, influenza vaccination was included in the National Immunization Program since 2004. However, the population health effects of the vaccine on children have not been fully described. Thus, we estimated the impact of influenza immunization in terms of...

Human cytomegalovirus (HCMV) infection in children and young adults has been associated with changes in the innate immune system. We herein analyzed the possible effect of very long term HCMV infection on the expression of several NK cell receptors. Ninety HCMV-seropositive individuals were included and classified as young adults (n=30), elderly (n...

Background: Respiratory syncytial virus (RSV) is a leading etiological agent of acute respiratory tract infections and hospitalizations in children. However, little information is available regarding RSV infections in Latin American countries, particularly among adult patients. Objective: To describe the epidemiology of RSV infection and to analyze...

Background. We sought an alternative design to a typical ILI observational study design in order to determine if circulating respiratory viruses were causing increased disease severity. An observational study was conducted to provide systematic sampling and real-time information regarding the pathogens causing ILI and SARI among patients seeking ca...

We report the complete genome sequence of the first Mexican human coronavirus (HCoV) OC43, obtained by new-generation sequencing and a metagenomic approach, isolated from a child hospitalized with pneumonia. The genome is closely related to the other OC43 genome sequences available, ranging from 99.8% to 98.2% nucleotide sequence identity.

Human rhinoviruses (HRVs) frequently cause mild upper respiratory tract infections, although there may be more severe disease manifestations including bronchiolitis and asthma exacerbations. Even though HRVs have been widely studied, many aspects of their role in respiratory infections remain unclear. HRV is classified into 3 species (A,B,C) within...

Background: The global surveillance of influenza has yielded a substantial amount of sequence and antigenic information covering the evolution of the virus. From 2010 to date, The Mexican Emerging Infectious Diseases Clinical Research Network (LaRed) implemented a hospital-based program to study the clinical and epidemiologic characteristics of inf...

Human cytomegalovirus (CMV) represents an important public health concern as it is associated with severe morbidity and mortality in transplant recipients, HIV-infected individuals and pregnant women given the risk of congenital infection. Congenital CMV is a leading cause of neurological sequelae, developmental delay and birth defects worldwide. C...

Background: The global burden of pediatric severe respiratory illness is substantial, and influenza viruses contribute to this burden. Systematic surveillance and testing for influenza among hospitalized children has expanded globally over the past decade. However, only a fraction of the data has been used to estimate influenza burden. In this ana...

Summary of influenza-associated ALRI and total number of influenza-associated hospitalizations in children 0–5 mo and 6–11 mo, with reference list. (DOCX)

Forest plot of data sources with PCR testing for pooled estimate, children <5 y. (TIFF)

Forest plot of data sources with PCR testing for pooled estimate, children <18 y. (TIFF)

Forest plot of data sources with PCR testing for pooled estimate, children <6 mo. (TIFF)

Forest plot of data sources with PCR testing for pooled estimate, children <1 y. (TIFF)

(DOC)

Literature search methodology and results, by database. (DOCX)

Summary of published articles included in the analyses, with reference list. (DOCX)

Analysis dataset. (CSV)

Forest plot of data sources with PCR testing for pooled estimate, children <2 y. (TIFF)

Forest plot of data sources with PCR testing for pooled estimate, children 5–17 y. (TIFF)

We sought an alternative design to a typical ILI observational study design in order to determine if circulating respiratory viruses were causing increased disease severity. An observational study was conducted to provide systematic sampling and real time information regarding the pathogens causing ILI and SARI among patients seeking care at the La...

Main conclusion: A RhoA-derived peptide fused to carrier molecules from plants showed enhanced biological activity of in vitro assays against respiratory syncytial virus compared to the RhoA peptide alone or the synthetic RhoA peptide. A RhoA-derived peptide has been reported for over a decade as a potential inhibitor of respiratory syncytial viru...

Room: Poster Hall Background: Mexico was the epicenter of the novel innuenza A/H1N1 2009 pandemic. This study evaluates the evolution of innuenza A/H1N1 pdm09 virus strains and the characteristics of associated-illness during sequential innuenza seasons after the pandemic.

The viral infectivity factor (Vif) is an HIV accessory protein that counteracts host anti-viral proteins of the APOBEC3 family. Accumulating evidence highlights the pivotal role that accessory HIV proteins have on disease pathogenesis, a fact that has made them targets of interest for novel therapeutic and preventative strategies. Little is known a...

SDS-PAGE of LickM-fusion and CP-fusion proteins after purification. (A) Coomassie gel of LickM fusion protein (PR-RhoA2-FL-F-3-HK). M: Molecular marker (See blue); 1: Elution fraction 100 mM imidazole; 2: Elution fraction 300 mM imidazole; 3: Elution fraction 500 mM imidazole. (B) Coomassie gel of CP-fusion proteins; M: Molecular marker (See blue);...

Background: Mexico was the epicenter of the novel influenza A/H1N1 2009 pandemic. This study evaluates the evolution of influenza A/H1N1 pdm09 virus strains and the characteristics of associated-illness during sequential influenza seasons after the pandemic. Methods: From April 2010-April 2014, a cohort of 5662 children and adults who sought medic...

Background: Mexico was the epicenter of the novel influenza A/H1N1 2009 pandemic. This study evaluates the evolution of influenza A/H1N1 pdm09 virus strains and the characteristics of associated-illness during sequential influenza seasons after the pandemic. Methods: From April 2010-April 2014, a cohort of 5662 children and adults who sought medic...

Background: Human Rhinoviruses (HRV) are frequent pathogens among children, mostly associated with common cold; however, recently it has been classified in three different species (HRVA, HRVB and HRVC); it has been suggested that HRVC could be associated with more severe disease. Methods: The ILI002 Study was an observa%onal study performed by LaRe...

Certain genotypic combinations of killer-cell immunoglobulin-like receptors (KIR) and human leukocyte antigens (HLA) have been associated with favourable outcomes after exposure to human immunodeficiency virus in Caucasoid and African populations. Human immunodeficiency virus (HIV) infection is characterized by a rapid exhaustion of CD4 cells, whic...

Objective: The purpose of the study was to analyze the association between cytomegalovirus (CMV) infection in dental students with occupational risk factors and a genetic trait (NKG2C gene deletion). Study design: Case-control study. 176 students were included and divided in two groups according to CMV serological results: those with CMV infection...

Respiratory syncytial virus (RSV) is the leading cause of severe lower respiratory tract infection in infants. Reduced numbers of NK cells have been reported in infants with severe RSV infection; however, the precise role of NK cells during acute RSV infection is unclear. In this study we analyzed the NK and T cell phenotype as well as LILRB1 gene...

Cytomegalovirus (CMV) is widely distributed and constitutes the main cause of congenital infections worldwide. CMV transmission during pregnancy represents one of the major impacts of this virus on public health. This study aimed at assessing glycoprotein B (gB) CMV genotypes in Mexican children and pregnant women, since there is limited informatio...

Cytomegalovirus (CMV) is the most common cause of congenital infection in developed countries and a major cause of neurological disability in children. Although CMV can affect multiple organs, the most important sequelae of intrauterine infection are related to lesions of the central nervous system. However, little is known about the pathogenesis a...

Main conclusion The HRA2pl peptide expressed by transient transformation in N. tabacum plants is capable of inhibiting the binding of the human metapneumovirus to HEp-2 cells at the fusion stage. Human metapneumovirus (hMPV) is an agent responsible for acute respiratory infections that mainly affects children under 3 years, the elderly and immuno...

Background: Human respiratory syncytial virus (RSV) is the leading cause of respiratory tract infections in children globally, with nearly all children experiencing at least one infection by the age of two. Partial sequencing of the attachment glycoprotein gene is conducted routinely for genotyping, but relatively few whole genome sequences are av...

##Assembly-Data-START## Coverage :: 100% vif encoding region Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##

##Assembly-Data-START## Coverage :: 100% vif encoding region Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##

##Assembly-Data-START## Coverage :: 100% vif encoding region Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##

##Assembly-Data-START## Coverage :: 100% vif encoding region Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##

##Assembly-Data-START## Coverage :: 100% vif encoding region Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##

##Assembly-Data-START## Coverage :: 100% vif encoding region Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##

##Assembly-Data-START## Coverage :: 100% vif encoding region Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##

##Assembly-Data-START## Coverage :: 100% vif encoding region Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##

##Assembly-Data-START## Coverage :: 100% vif encoding region Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##