D N Nimesh Madushanka

Sabaragamuwa University of Sri Lanka | SUSL · Department of Livestock Production

Chicken eggs are one of the most common constituents in the Sri Lankan diets. Even though the eggs are rich in many essential nutrients, contaminated eggs can pose severe threat to the public health. Moreover, the antibiotic resistance development by bacteria has become a greater threat to the human and animal health in the globe and this is due to...

Bacterial biofilms are matrix-enclosed microbial accretions that adhere to biological or non-biological surfaces which play an important role in public health and food safety. Multi-species biofilms are more common in natural settings, on medical devices, on biological surfaces and in food industry. However, the interspecies interactions underlying...

Biofilm formation by many foodborne pathogens remained a serious concern for public health and food safety. Compared to mono-species biofilms, multi-species biofilms are more common in natural settings and in places where food is produced. This study was conducted to assess the biofilm formation by E. coli, Proteus, and Salmonella as mono and dual-...

Free living bacteria produce biofilms on biotic or abiotic surfaces as complex communities residing within an extracellular polymeric matrix as a survival mechanism thriving the harsh conditions. These biofilm forming bacteria get more attention as they are more virulent and resistant to antibiotics and sanitizers. This study isolated E. coli, Prot...

Due to increased morbidity and mortality, foodborne diseases are leading to greater public health significance. Food can be contaminated from the point of raw materials, during the production process and in the consumer channel. Further, these microorganisms have led to the development of resistance against commonly used antibiotics due to the impr...

Culture-based fisheries of Giant Freshwater Prawn (GFP), Macrobrachium rosenbergii have been introduced in Sri Lankan reservoirs, making economically important component in the inland fishery. Hitherto, microbial studies associated with GFP in reservoirs have not been reported. Present study is of utmost importance since it is a novel study conduct...

Aims: Investigate the interaction of Salmonella spp. with E. coli and Proteus spp. in biofilm formation as mono and dual-species at different time durations Experimental Design: Salmonella, Proteus, and E. coli were isolated from Broiler chicken meat, and the biofilm-forming ability of these organisms were studied. Place and Duration of Study: The...

Biofilms are microbial communities encased with self-producing extracellular matrix, composed with polysaccharide, DNA and protein. Persistent survival capability of these biofilms in food contact surfaces cause contamination of food batches and pose health risk among the public. The biofilm formation is affected by several environmental factors su...

Foodborne diseases are mainly associated with the consumption of meat and meat products contaminated with pathogenic microorganisms such as bacteria, viruses and parasites. Among them bacteria play a vital role. Salmonella and Escherichia coli are considered as leading causes of food-associated bacterial illness globally. Antimicrobials are the dru...

Biofilms are microbial community adhered to biotic or abiotic surfaces and embedded within an extracellular Polymeric matrix. They have potential to resist antibiotics and disinfectants, causing health concerns in medical and food industries. Biofilm formation is affected by nutrient availability, temperature and other environmental factors. The pr...

Biofilms are microbial associations as communities on a biotic or abiotic surface by secretion of matrix where they thrive in stressful environments. These communities can be formed by single species or by multiple species that is differing from their planktonic state and are capable of surviving on food contact surfaces. This process creates huge...

Kisspeptin, a neuropeptide and the master controller of reproductive axis upstream to GnRH neurons, and its receptor are also expressed in extrahypothalamic tissues, such as ovaries. As systemic kisspeptin has been shown to modulate follicular dynamics in cattle, we hypothesized that kisspeptin has direct actions on the ovarian follicular developme...

A biofilm is a microbial community attached to a surface which embedded in a self-producing complex extra polymeric matrix of polysaccharide, protein and DNA. Biofilms gain special attention as it causes chronic infection, chronic source of contaminations due to its role in development of resistance to antibiotics, disinfectants and resisting phago...

Initiation of folliculogenesis through the activation of primordial follicle and their development in the female ovary plays a vital role in determining the fertility and reproductive strength of the mammals. Follicle formation and their development in the mammalian ovary is a complex process regulated by the action of the hypothalamic-pituitary-go...

Vascular endothelial growth factor (VEGF) is well known for its ability to regulate angiogenesis where it has been reported of playing a crucial role in metastasis of tumors. Stem cell factor (SCF) plays a crucial role in cell viability. Growth of a mammalian oocyte starts with an avascular structure called primordial follicle' and after the ovulat...

Understanding how oocyte morphogens regulate folliculogenesis and how their actions and interactions are integrated into the overall processes in physiology and pathophysiology of reproductive systems of mammals is an existing challenge. Growth of an oocyte in mammal starts with an avascular structure called a 'primordial follicle' and subsequent t...

Folliculogenesis is a complex process of ovarian follicle growth and development, which is yet to be completely understood. Vascular endothelial growth factor (VEGF) is known for its ability to promote angiogenesis. Among different candidates, VEGF165a is recognized as one of the major factors that determines the degree of vascularisation of the ta...

Aims: The study was carried out to investigate the effect of natural compounds and organic acids on Salmonella typhimurium in broiler chicken meat. Study Design: This is a laboratory-controlled experimental design. Place and Duration of Study: Department of Livestock Production, Sabaragamuwa University of Sri Lanka, between February 2014 and April...

Aims: Nitrite is used as the main curing agent in the process of sausage production, but Nitrite has limited usage in food processing due to its, carcinogenic effect. Thus, much attention has been given to find alternative compounds to replace the Nitrite over the past decades. Nisin, a natural polypeptide compound extracted from Lactococcus lactis...

Staining is an integral part of modern histopathology and without proper staining histology would be a mere concept. Haemotoxylin and Eosin (H&E) stain have been evolved during last several decades and considered as the golden standard for histological examinations for paraffin or resin embedded animal tissues which have been fixed, processed, embe...

Mammalian folliculogenesis is a complex process which determines the availability of viable oocyte for successful fertilization. Initiation of mammalian primordial follicle development also known as primordial follicle activation is poorly understood. Vascular endothelial growth factor 165a (VEGFA165a) is a well-known angiogenic factor which stimul...

The reproductive performance and health parameters of dairy herds have a significant effect on the farm economy. Body condition score (BCS) and locomotion score (LMS) were used as performance indicators to assess the performance of dairy animals. Objective of the current study was to evaluate the effect of BCS and LMS to predict the reproductive an...

In intensive cattle farming, cows spend between 50% and 65% of their time lying down and bacteria can be transferred to the lying surface and the teats. Managing and maintaining of healthy udder of the dairy cows has a significant impact on farm economy. This is considered as one of the key points in sustainability of dairy farming. This increases...

The present study was conducted to elucidate the applicability of body condition score (BCS) and locomotion score (LMS) to predict the reproductive and health performance of cows in intensive manage dairy herd in upcountry dairy farm in Sri Lanka. BCS and LMS were scored using 1 to 5 point score system. Forty three (n=43) pregnant cows and forty si...

Foodborne illness caused by consumption of contaminated foods with pathogenic bacteria or their toxins has been of great concern to public health. Salmonella enteritica serovar Typhimurium is a common food-borne pathogen that causes the deadly salmonellosis in human. Human acquires S. Typhimurium through consumption of contaminated animal products....

Herbal medicines form an important part of health care system in humans because they are easily available, cheaper and safer than synthetic drugs. Plants produce a diverse array of bioactive molecules, making them a rich source of diverse types of medicines. In recent years, drug resistance to human pathogenic bacteria has been commonly reported fr...