Cristina Garcia-De La Peña

Universidad Juárez del Estado de Durango · Facultad en Ciencias Biológicas

The blood bacterial microbiota of the Texas tortoise (Gopherus berlandieri) in Tamaulipas, Mexico, was characterized by next-generation sequencing. In 2019, blood was collected from 6 free-living tortoises. DNA was extracted, the V3-V4 region of the 16S rRNA gene was amplified, and Illumina sequencing was performed. The results showed 9 phyla, 20 c...

Objective: The objectives of this study were to determine the richness, abundance, and diversity of bacteria in stray dogs (Canis lupus familiaris) infested by ticks in Comarca Lagunera, northern Mexico, and to establish their pathogenic and or/zoonotic potential. Materials and Methods: Blood samples from 12 dogs were collected, and their deoxyribo...

One of the most important economic activities in northern Mexico is the bovine livestock (beef and milk), on which many families in suburban and rural areas depend. In recent years, the number of bacteriological studies in these ruminants has increased due to the accessibility of molecular technologies. In the present study, the blood bacterial mic...

Understanding the long-lasting associations among humans, neglected dogs, tick-borne diseases, and core blood bacteria-related pathogenic taxa using next-generation sequencing, Int. J. One Health, 10(1): 63-73. Abstract Background and/or Aim: Dogs are long-lasting companion animals, and ticks are the most common external parasites in dogs. An incre...

Background and Aim: Dogs are long-lasting companion animals, and ticks are the most common external parasites in dogs. An increase in the population of neglected domestic dogs has increased the risk of contact with ticks, especially in places where tick-borne diseases (TBDs) are endemic. We aimed to characterize the bacterial blood profiles of peop...

Maize is an important cereal in terms of human and animal nutrition worldwide. In our study, the bacterial microbiome composition of the soil rhizosphere was compared among three maize (Zea mays L.) races: forage (C1), dual-purpose (C2) and grain (C3). Additionally, the impact of the bacterial microbiome of the soil rhizosphere over yield was deter...

The study took as a purpose to determine the Total Carbon (TC) content in the biomass, the Starch Carbon fraction (SC) and its annual dynamics in the biomass of perennial organs (stem and root) in adult trees of two of walnut tree ( Carya illinoinensis ). Four adult Western and Wichita tree stem and root samples were carried out monthly for a whole...

Objective: To evaluate the impact of extensive grazing in the accumulation of soluble sugars in Prosopis laevigata trees, whose leaves and fruits are directly consumed by cattle. Design/Methodology/Approach: The ejido Emiliano Zapata in Durango was the study area. Stem and root samples were collected from a stand of extensive grazing and a stand wi...

p> Background. Soil management practices modify the microbial communities and the carbon stocks (organic, inorganic, and total). The increase in microbiological communities’ diversity improves the production of plants; thus, it is essential to understand the predominant bacterial taxa in the soil. Objective. The objective of the present study was t...

La biodiversidad de la región de Cuetzalan en Puebla, México, se pueden definir como prioritaria para la conservación por la presencia de bosque mesófilo de montaña presente en las cañadas y la selva alta perennifolia en las partes bajas. Sin embargo, dados los requerimientos ambientales de ambos tipos de vegetación hay un nivel de fragmentación mu...

Analysis of the Intestinal and Faecal Bacterial Microbiota of the Cervidae Family Using 16S Next-Generation Sequencing: A Review. Abstract: The Cervidae family has a wide distribution due to its adaptation to numerous ecological environments, which allows it to develop a diverse microbial community in its digestive tract. Recently, research has foc...

La tortuga de Texas (Gopherus berlandieri) es la más pequeña del género y se distribuye desde el sur de Texas (EUA) hasta el noreste de México y San Luis Potosí. En el presente estudio se llevó a cabo un análisis de morfometría geométrica en tortugas adultas mantenidas en cautiverio en los estados de Coahuila, Durango, Nuevo León y Zacatecas, Méxic...

La información sobre la microbiota bacteriana oral de las tortugas acuáticas del género Trachemys es muy escasa. El objetivo del presente estudio fue describir la composición y abundancia de la microbiota bacteriana oral de la tortuga hicotea (Trachemys venusta) en el municipio de Agua Dulce, Veracruz, México empleando secuenciación masiva de sigui...

Existe poca información de las malformaciones en crías de tortuga marina a nivel internacional. En ciertos casos, estas condiciones anormales entorpecen el desempeño de la cría y en ocasiones son fatales, lo cual es un impacto negativo para la conservación de estas especies en peligro de extinción. La tortuga verde (Chelonia mydas) es la especie má...

The taxonomic determination of fecal bacteria is an important starting point to improve the strategies of management and conservation of wildlife. Here we report an approach to the fecal bacteria of the American bison (Bison bison) in Chihuahua, Mexico (Janos Biosphere Reserve). We used 16S rRNA (V3-V4) next generation massive sequencing (NGS) in I...

Actualmente, existe una creciente preocupación ya que algunas especies de anfibios han mostrado un declive en sus poblaciones por causa de diversos factores, entre ellos los metales pesados; por esta razón, es importante realizar estudios sobre esta problemática ambiental. El objetivo de este estudio fue determinar la cantidad del plomo y arsénico...

Objective: To evaluate the impact of extensive grazing in the accumulation of soluble sugars in Prosopis laevigata trees, whose leaves and fruits are directly consumed by cattle. Design/Methodology/Approach: The ejido Emiliano Zapata in Durango was the study area. Stem and root samples were collected from a stand of extensive grazing and a stand wi...

Existe poco conocimiento sobre la microbiota intestinal de las liebres del género Lepus. El objetivo del presente estudio fue describir la composición y abundancia de la microbiota bacteriana fecal de la liebre cola negra (Lepus californicus) en la Reserva de la Biósfera Mapimí, Mexico, empleando secuenciación masiva de siguiente generación. Se col...

Resumen. El maíz, Zea mays L., es de gran importancia económica, social y cultural en México. Su agroecosistema posee insectos fitófagos y entomófagos. Se estudió la diversidad de éstos en la Comarca Lagunera en 2020 y 2021. Se identificaron 38 especies de insectos entomófagos, tanto depredadores como parasitoides, siendo las más comunes Bariscapus...

Resumen. El gusano cogollero, Spodoptera frugiperda (J.E. Smith), es el insecto plaga de mayor importancia económica debido a los daños que causa al maíz (Zea mays L.) alrededor del mundo. Se determinaron las especies de parasitoides de S. frugiperda y el grado de parasitismo natural en maíz de la Comarca Lagunera, ubicada en Coahuila y Durango, Mé...

Resumen. El maíz, Zea mays L., es de gran importancia económica, social y cultural en México. Su agroecosistema posee insectos fitófagos y entomófagos. Se estudió la diversidad de éstos en la Comarca Lagunera en 2020 y 2021. Se identificaron 38 especies de insectos entomófagos, tanto depredadores como parasitoides, siendo las más comunes Bariscapus...

The aim of the current study was to determine the monthly total soluble sugar concentration in the stem and root of the Wichita and Western walnut (Carya illinoinensis) varieties during a productive cycle in northern Mexico. Vegetable samples were obtained using a systematic sampling method, and processed by means of a spectrophotometric assay to d...

In Mexico, information on the bovine fecal microbiota (Bos taurus) is scarce. The present study describes the diversity and abundance of bacteria in fecal samples from rangeland bovines, collected in the Mapimi Biosphere Reserve in the central part of the Chihuahuan desert. Fecal samples were analysed using high-throughput next generation massive s...

In Mexico, information on the bovine fecal microbiota (Bos taurus) is scarce. The present study describes the diversity and abundance of bacteria in fecal samples from rangeland bovines, collected in the Mapimi Biosphere Reserve in the central part of the Chihuahuan desert. Fecal samples were analysed using high-throughput next generation massive s...

A comprehensive evaluation of the sustainability of the Small Ruminant Production Systems (SRPS) in Latin America and the Caribbean (LAC) is crucial to better understand their main distinctive characteristics and to propose a methodological analysis to quantify the ecological, economic and social footprint of small ruminants in the LAC region; this...

Currently, the sustainability of the planet is threatened mainly by the excessive extraction of resources from ecosystems to meet the needs of a growing and more demanding human population. Although products of animal origin are an essential source of protein for humans and have shown great growth due to the increasing demand worldwide, this sector...

The sustainability of the goat production systems (GPS) must consider the quantification not only of the ecological footprint (ECOLF) but the carbon (CF), the blue water (BWF) footprints, their economic & social interactions, weighed by their economic value (EV). Our working hypothesis states that the GPS-ECOLF in Latin America & the Caribbean (LAC...

Total Soluble Sugars (ATS) are essential carbohydrates in growth, metabolism and survival mechanisms in stress conditions in decidous trees. The objective was to quantify ATS in Carya illinoinensis in different organs and phenological stages, in this specie and to be able to have an approximation to the adaptive capacity of the specie. Samples were...

Degraded vegetation is the result of a process that affects structural and functional characteristics. Tree species from the Acacia genus are very important to the ecosystem in semi-arid lands due to their participation in the recovery of highly degraded areas. One of the most important species among this genus is A. schaffneri. The status of a for...

La interacción entre epífitas y sus plantas hospederas comúnmente implica el desarrollo de las epífitas sin que éstas dañen a las hospederas. Sin embargo, recientemente se ha encontrado que algunas epífitas tienen efectos perjudiciales directos sobre sus anfitriones. En el ecosistema desértico, el mezquite funciona como sombra y refugio para la fau...

Actualmente existe poca información sobre el estado de conservación de las poblaciones de anfibios que habitan las zonas áridas de México. La cuenca de los ríos Nazas y Aguanaval, ubicada en la región árida y semiárida del norte-centro, resulta en particular interesante debido a las alteraciones que ha sufrido por la construcción de obras de captac...

En la actualidad, gran parte de los esfuerzos de conservación de fauna silvestre se lleva a cabo en zoológicos. Debido a que el control parasitario es un aspecto importante para el buen manejo sanitario de estos lugares, se determinaron los parásitos intestinales en doce especies de la familia Felidae y siete especies de la familia Canidae en ocho...

Se describen los primeros datos sobre abundancia y diversidad de bacterias de un pool de muestras fecales de gato montés (Lynx rufus) en la Reserva de la Biosfera Mapimí. Se realizó secuenciación masiva de la región V3-V4 del gen 16S rRNA con Illumina. Los resultados fueron analizados en QIIME usando como referencia taxonómica la base de datos de E...

The objective of the present study was to estimate the prevalence of parasites and identify the ones with the zoonotic potential present in the feces of households dogs and stray dogs in Gomez Palacio, Durango, Mexico. Fifty fecal samples from household dogs and 50 fecal samples from stray dogs were collected and analyzed by sucrose flotation and M...

The Mexican wolf (Canis lupus baileyi) is an endangered canine. Both Mexico and the United States are currently collaborating to reproduce and reintroduce individuals to their original habitats. However, keeping these wolves in captivity represents a great commitment to meet their basic needs. Diet is a determining factor that is closely related to...

El nitrógeno es uno de los elementos nutritivos más importantes para el crecimiento y desarrollo de las plantas. Este se almacena en los órganos perennes en forma de proteínas durante el otoño e invierno y se moviliza hacia los brotes durante la primavera. El presente estudio pretendió determinar qué variables ambientales tienen un efecto en el con...

Pecan tree (Carya illinoinensis Koch) is a native specie in Northern Mexico and the Southeastern United States, both countries dominate the production of walnuts to worldwide. The objective was to determine starch balance in root and trunk monthly in two varieties (Western and Wichita), during a production cycle. Sampling was systematic. Results in...

El cambio climático puede influenciar la frecuencia, severidad y extensión de los incendios forestales. Los incendios son perturbaciones comunes en el medio ambiente que pueden tener repercusiones ecológicas y económicas graves e impredecibles; no obstante, también es un conductor importante del funcionamiento del ecosistema, la dinámica de la biot...

Se describe la abundancia y diversidad de bacterias fecales de un pool de muestras de caballo en libre pastoreo en la reserva de la Biosfera Mapimí. Se realizó secuenciación masiva V3-V4 del gen 16S rRNA con Illumina. Los resultados se analizaron con QIIME y la referencia taxonómica EzBioCloud. Se identificaron 10 phyla, 16 clases, 26 órdenes, 46...

Agronomic management modifies the soil bacterial communities and may alter the carbon fractions. Here, we identify differences in several chemical and biological soil variables, as well as bacterial composition between organic (Org) and conventional (Conv) agronomic management in pecan (Carya illinoinensis) orchards located in Coahuila, Mexico. The...

The general bacterial microbiota of the soft tick Ornithodoros turicata found on Bolson tortoises (Gopherus flavomarginatus) were analyzed using next generation sequencing. The main aims of the study were to establish the relative abundance of bacterial taxa in the tick, and to document the presence of potentially pathogenic species for this tortoi...

Resumen. En el 2002 se evaluaron los ciclos de actividad diaria y estacional de los saurios Uta stejnegeri, Uma exsul y Aspidoscelis marmorata en un hábitat de dunas al suroeste de Coahuila, México. Se recorrió un transecto de 500 m, cada hora (de 0700 a 1900 h) durante 7 días, en primavera, verano y otoño. Se registró el número de individuos activ...

Morphometric characteristics of blood cells were examined in 44 (16 males, 28 females) Bolson tortoises (Gopherus flavomarginatus) in Mapimí, Mexico. Blood samples were drawn from the subcarapacial vein, and smear stains were used to differentiate and measure the diameter and surface area of cells. Mature and polychromatophilic erythrocytes were id...

Introduction: Common variable immunodeficiency (CVID) is the main symptomatic primary immunodeficiency and is associated with complex immune disorders. Gut microbiota interacts closely with the immune system, and intestinal dysbiosis is related to multiple diseases. Objective: To describe for the first time the composition of gut microbiota in Mexi...

Este estudio presenta la expresión fenotípica en cuanto a coloración, el com- portamiento de la concentración de azúcares solubles en dos etapas fenológicas (desarrollo vegetativo y dormancia) en caladio (Caladium bicolor), así como la presencia/ausencia de polipéptidos de reserva en tubérculos bajo tratamientos de 4 y 8 mL•L-1 de productos orgánic...

The aim of this study was to determine the soil microbiome throughout mass sequencing in coffee plantations managed with either an organic (OAM; i.e., bio-fertilizers Azospirillum brasilense and Glomus intraradices) or a conventional (CAM; i.e., traditional NPK-fertilization) agronomic systems. Soil microbiome samples were collected in tropical eas...

The possible out-of-season effect of beta-carotene supplementation on ovulation rate (OR), antral follicles (AFN), and total ovarian activity (TOA = OR + AFN) as related to the LH release pattern in yearling anestrous goats was evaluated. In late April, Alpine-Saanen-Nubian x Criollo goats (n = 22, 26 N) were randomly allotted to: (1) Beta-carotene...

Introduction: Common variable immunodeficiency (CVID) is the main symptomatic primary immunodeficiency and is associated with complex immune disorders. Gut microbiota interacts closely with the immune system, and intestinal dysbiosis is related to multiple diseases. Objective: To describe for the first time the composition of gut microbiota in M...

Uma exsul is a micro-endemic species of the Chihuahuan desert, listed as an endangered species in NOM-059-SEMARNAT-2010. The objective was to determine the genetic population structure of U. exsul, assuming that the distribution allopatric populations constitute different lineages. Methodology: 14 field trips where carried out, collecting 24 biolog...

We present findings of our preliminary study to determine biometry and blood chemistry values of healthy wild individuals of the critically endangered Bolson tortoises (Gopherus flavomarginatus) in Mexico. Given the absence of previously published data regarding hematology parameters for this species, these results represent an important base for a...

Research analyzing fecal microbiota provides important insights into the biological mechanisms affecting the health of animals. The Bolson Tortoise (Gopherus flavomarginatus) is endemic to northern Mexico and is threatened mainly by habitat loss and collecting by humans. Here, we characterized the fecal bacterial communities of wild (10 samples) an...

El transporte de azúcares de tejidos de asimilación a tejidos de reserva determina el crecimiento o el almacenamiento reproductivo vegetal. El objetivo fue determinar las concentraciones de azúcares solubles en nogal pecanero crecidos en una huerta ubicada en Torreón, Coahuila, durante las etapas de producción y letargo. Se tomaron muestras de raíz...

Transport of sugars from assimilation tissues to reserve tissues determines growth or reproductive storage. The objective was to determine the concentrations of soluble sugars in pecan tree grown in an orchard located in Torreón, Coahuila, during the stages of production and dormancy. Samples of root, stem, branch, and bud emitted from the year (an...

Mosquitoes are the most important arthropods from the point of view of public health, due to the fact that they can transmit a large number of pathogens which can cause diseases to humans and animals. Aedes aegypti (L.) is one of the most important vector species in the world, since it can transmit numerous pathogens such as dengue, Zika, and chiku...

In this study, we present the first records for the occurrence of Batrachochytrium dendrobatidis (Bd), in anurans from the Nazas-Aguanaval basin in the north-central México, as well as the first records of this pathogen infecting Anaxyrus cognatus, A. debilis, A. punctatus, and Gastrophryne olivacea. We obtained 169 skin swab samples, in which Bd w...

Introduction: The pecan tree (Carya illinoinensis K. Koch) is a species native to northern Mexico and the Southeastern United States; the two countries dominate pecan production worldwide. Objective: To determine the influence of temperature and irradiation on the starch concentration in the root and trunk of C. illinoinensis varieties Wichita and...

We evaluated the possible effect of targeted supplementation of protein enriched Opuntia megacantha Salm-Dyck cladodes, the flat leaf-like stem of cactus species, upon corpus luteum number (CLN), corpus luteum diameter (CLD), serum progesterone concentrations (P4) and embryo implantation rate (EIMPL) in anestrous goats exposed to sexually active ma...

Introduction: Common variable immunodeficiency (CVID) is the main symptomatic primary immunodeficiency and is associated with complex immune disorders. Gut microbiota interacts closely with the immune system, and intestinal dysbiosis is related to multiple diseases. Objective: To describe for the first time the composition of gut microbiota in M...

En la Comarca Lagunera se estima que la superficie sembrada de nogal (Carya illinoensis) es de 9,208 ha-1, con un rendimiento aproximado de 1, 459 t de nuez ha-1. Las variedades que predominan en la Comarca Lagunera son Wichita y Western. Cuando la demanda de elementos nutritivos en las plantas no puede ser cubierta, estas dependen directamente de...

The community of oral bacteria of the Bolson tortoise, Gopherus flavomarginatus, was determined at the Reserva de la Biosfera Mapimí, México by massive sequencing. During 2015, samples of 5 males and 5 females apparently healthy were collected; DNA was extracted, V3-V4 16S rRNA regions were amplified, sequencing was carried out with Illumina, and E...

The community of oral bacteria of the Bolson tortoise, Gopherus flavomarginatus, was determined at the Reserva de la Biosfera Mapimí, México by massive sequencing. During 2015, samples of 5 males and 5 females apparently healthy were collected; DNA was extracted, V3-V4 16S rRNA regions were amplified, sequencing was carried out with Illumina, and E...

Animal Biodiversity and Conservation. 2019; 42(1):127-134.

Intestinal bacteria are an important indicator of the health of their host. Incorporating periodic assessment of the taxonomic composition of these microorganisms into management and conservation plans can be a valuable tool to detect changes that may jeopardize the survival of threatened populations. Here we describe the diversity and abundance of...

El objetivo de este trabajo fue determinar la composición taxonómica de la microbiota bacteriana fecal del ratón de bolsillo chihuahuense, Chaetodipus eremicus, en la Reserva de la Biósfera Mapimí . Resultados: Se registraron 10 phyla, 12 clases, 20 órdenes, 32 familias y 178 géneros. La abundancia taxonómica estuvo representada por el phylum Firmi...

Se generó un registro de 10 phyla, 13 clases, 21 órdenes, 35 familias y 236 géneros. La abundancia taxonómica estuvo representada por el phylum Firmicutes (89%). La clase Clostridia (89%) se encontró representado por el orden Clostridiales (89%). Dentro de este orden la familia Ruminococcaceae (44%) y Lachnospiraceae (42%) fueron las más abundantes...

Hidrocoras seeds usually have some type of structures for dispersion in water. The structures may have less density than water, low specific weight, chambers of air, spongy tissue and small cotyledons with spongy tissue. This study evaluated the ability of buoyancy of Astrophytum coahuilense seeds and the structures that give this feature. The dens...

Hidrocoras seeds usually have some type of structures for dispersion in water. The structures may have less density than water, low specific weight, chambers of air, spongy tissue and small cotyledons with spongy tissue. This study evaluated the ability of buoyancy of Astrophytum coahuilense seeds and the structures that give this feature. The dens...

The possible effects of betacarotene (BC) supplementation on the secretion pattern of growth hormone (GH) and insulin-like growth factor-1 (IGF-1), and their possible relationship with total ovarian activity (TOA), was evaluated in adult goats during the breeding season. In October, goats [n=22, 3.5y. old, 7/8 Sannen-Alpine] were randomly assigned...

Se realizó un análisis de proteínas totales solubles y de proteínas de reservas vegetativas (PRV), en tejidos de árboles de nogal (Carya illinonensis Koch var. Western) en letargo y producción. La determinación de proteínas totales solubles se realizó por el método de Bradford, por medio de electroforesis en gel de poliacrilamida al 15% para detect...

El estudio se llevó a cabo en Torreón, Coahuila. con la finalidad de analizar la dinámica del almidón (mg g-1 MS) y su contribución al carbono total de la biomasa en los órganos perennes del nogal (Carya illinoensis Koch) en dos etapas fenológicas. El muestreo fue sistemático. Los resultados mostraron diferencia significativa en la concentración de...

The study was carried out in Torreon, Coahuila, with the purpose of analyzing the starch dynamics (mg g-1 MS) and its contribution to the total carbon of the biomass in the perennial organs of the walnut tree (Carya illinoensis Koch) in two phenological stages. The sampling was systematic. The results showed a significant difference in the concentr...

The study was carried out in Torreon, Coahuila, with the purpose of analyzing the starch dynamics (mg g-1 MS) and its contribution to the total carbon of the biomass in the perennial organs of the walnut tree (Carya illinoensis Koch) in two phenological stages. The sampling was systematic. The results showed a significant difference in the concentr...

The study was carried out in Torreon, Coahuila, with the purpose of analyzing the starch dynamics (mg g-1 MS) and its contribution to the total carbon of the biomass in the perennial organs of the walnut tree (Carya illinoensis Koch) in two phenological stages. The sampling was systematic. The results showed a significant difference in the concentr...

An analysis of total soluble proteins and vegetative storage proteins (VSPs) in the tissues of pecan (Carya illinonensis Koch var. Western) trees in dormancy and production was carried out. Total soluble proteins were determined using the Bradford method, by means of electrophoresis in 15% polyacrylamide gel, to detect the VSPs present in the plant...