Cristhian Aponte

Universidad Técnica de Machala · Facultad de Ingeniería

The Juvenile idiophatic arthritis in spite of his low frequency, is the most frequent rheumatic inflammatory chronic disease in the infancy. Now we have precise and simple criteria to diagnose her in Primary care since they are criteria fundamentally clinical and spread on the time. The diagnosis of monkey or polyarthritis, in absence of infectious...

Juvenile idiopathic arthritis in spite of his low frequency is the most frequent rheumatic inflammatory chronic disease in childhood. We have now precise and simple criteria to diagnose it in Primary care since they are fundamentally clinical criteria. The diagnosis of mono-or polyarthritis, in absence of general infectious symptoms and with normal...

The Savannah River National Laboratory (SRNL) will perform lab analyzes on samples from the June 2009 THOR Fluidized Bed Steam Reforming (FBSR) Engineering Scale Test Demonstration (ESTD) Phase 3 with Tank 48H simulant at the Hazen Research Inc. (HRI) in Golden, Colorado. The purpose of the 2009 ESTD Phase 3 testing at the Hazen facility is to demo...

The detailed quantitative characterization of soft-tissue in-growth into highly porous artificial implants is critical to understanding the biophysical processes that will lead to the best structural scaffolding construct. Previous studies have performed mechanical peel tests and mostly qualitative histological analyses of soft-tissue. The goal of...

Replication of the rotavirus genome involves two steps: (i) transcription and extrusion of transcripts and (ii) minus-strand RNA synthesis in viral complexes containing plus-strand RNA. In this study, we showed evidence for the importance of the viral nonstructural protein of rotavirus, NSP2, in the replication of viral RNAs. RNA-binding properties...

The genomes of viruses in the family Reoviridae consist of segmented double-stranded RNA. There are 10 to 12 segments depending on the genus. The 5′ ends and the 3′ ends of the RNAs present conserved motifs for each virus genus. These conserved motifs have been hypothesized to play a role in genomic segment assortment during virus morphogenesis. Us...

Interaction between viral proteins and RNAs has been studied in rotavirus-infected cells. The use of UV cross-linking followed by immunoprecipitation and labeling with T4 polynucleotide kinase allowed us to detect interactions between RNA and nonstructural viral proteins. The RNAs linked to the nonstructural protein NSP3 have been identified as rot...

Studies on rotavirus non-structural proteins have been hampered in the past by difficulties in obtaining monospecific reagents. To make such reagents available, we have expressed in the baculovirus system NSP2 and NSP3 (formerly called NS35 and NS34, respectively) of the bovine rotavirus RF and produced hybridomas against these proteins. Full-lengt...

Intermolecular interactions between polypeptide chains often play essential roles in such biological phenomena as replication, transcription, translation, transport, ligand binding, and assembly. We have initiated studies of the functions of the rotavirus SA114F gene 7 product by sequence analysis and expression in insect cells. This nonstructural...

The Savannah River Site (SRS) must empty the contents of Tank 48H, a 1.3 million gallon Type IIIA HLW storage tank, to return this tank to service. The tank contains organic compounds, mainly potassium tetraphenylborate that cannot be processed downstream until the organic components are destroyed. The THOR{reg_sign} Treatment Technologies (TTT) Fl...